RU2624845C1 - Disinfectant with washing effect - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: invention is intended for washing and disinfecting laboratory utensils, surgical instruments for veterinary and medical purposes. The disinfectant comprises lactic acid of 10.0-25.0 wt %; nonionic surfactant of 0.25-0.5 wt % and distilled water up to 100 ml.

EFFECT: invention use provides high disinfecting and washing activity, the possibility of combining disinfection and pre-sterilisation cleaning, ease of manufacturing and ease of applying.

3 ex

Description

The invention relates to the field of disinfection and can be used for disinfection and washing of laboratory glassware, surgical instruments of veterinary and medical purposes, as well as for pre-sterilization cleaning, including combined with the disinfection of products of veterinary and medical purposes.

Known detergents and disinfectants, which are mixtures of synthetic detergents, mainly anionic and bactericidal chlorine-containing substances. Among these tools is desmol [1] (ed. Certificate. No. 223979, class C11D 3/065, 1966). Desmole contains a surfactant - the substance sodium tripolyphosphate, sodium metasilicate, chloramine, soda ash, sodium sulfate and water.

However, this tool is unstable during storage, has the smell of chlorine and irritates the integumentary tissues of humans and animals, which limits its scope. The systematic use of the product is accompanied by the formation of deposits and films on the treated surfaces.

Known biocidal agent containing a peroxide compound (perhydrol or hydropyrite), a quaternary ammonium base (HOUR) and a hydrochloric salt of a high molecular weight tertiary amine [2] (SU No. 1587725, 1995).

A disadvantage of the known composition is the presence in the biocidal medium of salts of amines, as well as hydrogen peroxide, leading to increased toxicity of the proposed composition, which excludes its use in veterinary medicine, medicine and the food industry.

Known non-toxic detergent disinfectant containing mass. %: dodecyldimethylbenzylammonium chloride - 18-80, tetradecyldimethylbenzylammonium chloride - 18-80, tertiary amine oxide - 1-5 [3] (patent RU No. 2124557, 1999).

The disadvantage of this invention should be recognized as the harmful effects of a substance at concentrations above 0.2% on exposed skin surfaces, and this causes difficulties for personnel when working with this detergent and disinfectant.

Also known is a disinfectant containing alkyldimethylbenzylammonium chloride, an aqueous solution of acetic acid and sodium hydroxide [4] (RF patent No. 23335301, IPC A61L 2/16, 2008).

The disadvantage of this composition is the lack of effectiveness, causing the need for a long time of disinfection.

Known disinfectant containing an aqueous solution of a mixture of Quaternary ammonium salts (HOUR) [5] (RF patent No. 2330683, IPC A61L 2/16; C11 D 3/48, 2008).

The disadvantage of this composition is the insufficient washing effect.

Closest to the proposed is a disinfectant with a washing effect, including a disinfecting agent - salts of polyhexamethylene guanidine or polyhexamethylene biguanidine and additionally N, N-bis- (3-aminopropyl) -dedecylamine in a mass ratio of 1: 1-2, nonionic surfactant - water-soluble ethoxylated monoalkylphenols based on propylene trimmers or block copolymers of ethylene glycol and propylene glycol, dye, perfume and water [6] (patent RU No. 2172637, IPC A61L 2/16, published on 08.27.2001).

However, this disinfectant with a detergent effect has a complex composition, the presence of imported components, a high content of nonionic surfactant (nonionic surfactant) to 10, requiring long-term rinsing.

The present invention is the creation of a new simple, inexpensive, liquid disinfectant and detergent domestic products with high disinfecting and washing ability with a low content of nonionic surfactants.

The problem is solved in that a disinfectant with a detergent effect containing a disinfecting agent, nonionic surfactants and water, contains lactic acid as a disinfectant, and distilled water as water in the following ratio of components, wt. %: lactic acid - 10.0-25.0, nonionic surfactant - 0.25-0.5, distilled water - up to 100 ml.

Distinctive features of the proposed funds are the use of lactic acid as a disinfectant, and distilled water as the water in the following ratio, wt. %: lactic acid - 10.0-25.0, nonionic surfactant - 0.25-0.5, distilled water - up to 100 ml. This allows you to get a simple, inexpensive, liquid disinfectant and detergent with a high disinfecting and washing ability with a low content of nonionic surfactants to 0.5, as well as pre-sterilization cleaning of laboratory glassware, veterinary and medical instruments.

As a disinfecting agent used lactic acid - CH ₃ CH (OH) COOH GOST 490-79.

Lactic acid CH ₃ CH (OH) COOH GOST 490-79. For external use, it has a disinfectant (10-30% solution), keratolytic (10% solution), cauterizing (10-40% solution) effect. This weak acid has low toxicity and is practically harmless even in doses 8 times higher than recommended. Lactic acid is a natural product and can be considered as a biologically safe product. Lactic acid is characterized by high diffusion properties, moderate acidifying ability, strong antimicrobial action [7, 8]. It does not irritate the skin and mucous membranes; it is resistant to storage and to high temperatures. It does not fix organic impurities, it perfectly cleans both fresh and old, highly dried impurities from surfaces.

In veterinary medicine it is used as an anti-fermentation agent with an antiseptic effect to suppress conditionally pathogenic microflora of the gastrointestinal tract, as well as a disinfectant for aerosol disinfection in the presence of animals. They are used for air disinfection of poultry facilities in the presence of a bird with infectious laryngotracheitis, respiratory mycoplasmosis. As an antiseptic for trichomoniasis in cattle. As a cauterizing agent, it has been shown to remove neoplasms [8].

Lactic acid has a pronounced anti-fermentative effect for douching the vagina with trichomoniasis in cattle (1% solution), as a cauterizing agent for ulcerative lesions of the skin and mucous membranes (10-75% solutions). Lactic acid also has keratolytic properties and is included in the composition (up to 10-30%) of anti-corn agents.

In the external environment, lactic acid has a detrimental effect on microorganisms. It has antimicrobial activity against various gram-negative and gram-positive microorganisms, tuberculosis pathogens, respiratory viruses, Candida and Trichophyton fungi, and mold fungi.

As nonionic surfactants, the product contains water-soluble surfactants, in particular, ethoxylated monoalkylphenols based on propylene trimmers - neonol AF 9-10, neonol AF 9-12 (TU 2483-077-05766801-98), which are transparent liquids containing the main substances 97.5-98.0%, which have increased biodegradability. Nonionic surfactants have the greatest foaming ability [9, 10].

And as a solvent used distilled water.

To obtain the proposed composition, lactic acid is poured into a measuring cup, a nonionic surfactant and distilled water are added, mixed, poured into a glass container and used or hermetically sealed and stored for three years.

The resulting product has good washing and disinfecting properties, has bactericidal activity and is intended for the disinfection and presterilization of laboratory glassware, veterinary and medical instruments, without causing corrosion and destruction of materials. The tool is non-combustible, fire and explosion proof, environmentally friendly. The proposed tool does not irritate the skin and mucous membranes of the eyes, does not have sensitizing activity.

The following are examples illustrating the invention.

Example 1. Prepared a disinfectant with a detergent effect. They took a measuring cup, poured 10 ml of lactic acid, added 0.25 ml of neonol AF 9-10 or neonol AF 9-12, added to 100 ml of distilled water and used with wetting for degreasing, disinfection and washing of laboratory glassware and veterinary instruments. Laboratory glassware and instruments (test tubes, Petri dishes, slides, pipettes) are clean, free of fat-soluble contaminants and without microorganisms.

Example 2. A disinfectant and detergent were prepared, 15 ml of lactic acid were taken, 0.35 ml of neonol AF 9-10 or neonol AF 9-12 was added, topped up to 100 ml of distilled water and used as in example 1. The dishes were clean, without greasy contamination and without microorganisms.

Example 3. Prepared a disinfectant and detergent, took 25 ml of lactic acid, 0.5 ml of neonol AF 9-10 or neonol AF 9-12, topped up to 10 ml of distilled water, used similarly to example 1. The dishes are clean, without grease and without microorganisms.

Wetting, degreasing and disinfection was carried out for 25-30 minutes in a working solution. Then it was washed in two containers with distilled water and placed in an oven to 30 ° C.

The washing results were evaluated visually - the dishes and tools are clean, without smudges, shiny.

For laboratory studies, liquid pure cultures of Esherichia coli, Stafilococcus aureus, Salmonella cospora lactis, Pseudomonas aeruginosa, which are the most dangerous representatives of seeding microflora, were prepared. Samples of a disinfectant prepared in accordance with Examples 1-3 were added to test tubes with pure cultures. The prepared mixtures were mixed and left at room temperature for 50 minutes, after which crops were sown on solid nutrient media. After 12-48 hours of incubation at 37 ° C, none of the solid nutrient media showed an increase in the cultures sown on them treated with a disinfectant solution.

A solution containing less than 10 ml of lactic acid and less than 0.25 ml of nonionic surfactant weakly disinfects and does not completely clean the surface, and a solution containing more than 25 ml of lactic acid and more than 0.5 ml of nonionic surfactant is impractical from the point of view of economy.

Thus, the proposed disinfectant with a detergent effect exhibits high activity of both disinfectant and detergent, is simple and cheap to manufacture, convenient to use, affordable, has wetting, degreasing, disinfecting properties and a low surfactant content.

The proposed disinfectant with a washing effect is used in the laboratories of the Federal State Budgetary Institution “FTsTRB-VNIVI” in Kazan.

Information sources

1. USSR author's certificate No. 223979, cl. C11D 3/065, 1966.

2. Patent SU No. 1587725, 1995.

3. Patent RU No. 2144557, 1999.

4. RF patent №2335301, 2008.

5. RF patent No. 2330683, 2008.

6. Patent RU No. 2172637, 2001.

7. Chervyakov D.K., Evdokimov P.D., Vishker A.S. Medicines in veterinary medicine. M., Kolos, 1977, - S. 493.

8. Sokolov V.D., Rabinovich M.I., Gorshkov G.I. et al. Ed. V.D. Sokolova. - M: Kolos, 1997 .-- 543 p. (Textbooks and teaching aids for students of higher educational institutions).

9. Voloshchenko O.I., Wise I.V. Hygienic value of surfactants. K .: Health, 1991, - 176 p.

10. Novikov Yu.V. Ecology, environment and man: Textbook. pos. for universities, secondary, schools and colleges. - M .: Fair-press, 2002 .-- 560 p.

Claims (2)

A disinfectant with a washing effect, including a disinfectant, a nonionic surfactant and water, characterized in that it contains lactic acid as a disinfectant, and distilled water as water in the following ratio, wt. %: Lactic acid 10.0-25.0 Nonionic Surfactant 0.25-0.5 Distilled water up to 100 ml