RU2253466C1 - Method for preventing and treating endometritis in cows and sows - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: it is suggested to apply a preparation based upon hydrophilic fraction of porcine placenta and isotonic solution of sodium chloride at the following ratio of components, weight%: hydrophilic fraction of porcine placenta 2-3, isotonic solution of sodium chloride - the rest. The suggested ratio of components enables to optimize complex immunostimulating, antiphlogistic and adaptogenic action of the suggested preparation.

EFFECT: higher efficiency of prophylaxis and therapy.

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Description

The invention relates to the field of veterinary medicine and can be used for the prevention and treatment of endometritis in cows and sows.

A known method for the prevention and treatment of gynecological diseases in cows and sows using a biogenic stimulator in injection lekform based on placental tissues of the same animals after chemical treatment of the placenta with oxygen compounds of chlorine in a known manner. The drug by the effectiveness of the pharmacological action does not differ from known analogues based on human placenta. / Patent RU 2036651, A 61 K 35/50, publication date of the formula 1995.06.09 /.

Known anti-inflammatory drug for the prevention and treatment of postpartum complications in cows and other farm animals based on placental tissues. The anti-inflammatory drug is prepared from human placental tissue - the umbilical cord - with the addition of sterile distilled water in the ratio (1: 0.8) - (1: 1.2). / Patent RU 2035910, IPC 6 A 61 K 35/50, publication date of the formula 1995.05.27 /.

The closest analogue, as well as the most frequently used, is a method for the treatment and prevention of gynecological diseases in cows and sows using a drug - denatured emulsified placenta (PDE), which is made from human placenta. The drug is a sterile emulsion of a white or yellowish-white color, a specific smell. / Veterinary preparations in Russia: reference book / I.F.Klenova, N.A. Yaremenko - M .: Selkhozizdat, 2000. - p.446 /.

The disadvantages of the known methods is the use of raw materials - human placenta, resulting in low satisfaction of the needs of veterinary medicine, as well as species incompatibility of protein and polysaccharide components of the drug. The disadvantages of the PDE drug are the low effectiveness of the action, the reason for which is the use of outdated technologies for its production. The methods for obtaining this drug are based on processes:

- denaturation of placental tissues with oxidizing agents;

- hydrogen peroxide

These processes are aimed at the destruction of valuable biologically active substances contained in placental tissue.

The technical result of the invention is improving efficiency.

The technical result is achieved using a specially selected hydrophilic fraction of porcine placenta.

The drug is made as follows.

Pork placenta is washed with running water, fragmented and subjected to rapid freezing in pairs of liquid nitrogen to a temperature of (-80 ° C) - (-120 ° C) at a speed of 30 degrees per minute.

Cryopreservation of pork placenta is carried out in a cryo-mill during cooling with liquid nitrogen vapor to temperatures (-80 ° C) - (-120 ° C). The degree of tissue grinding is 20-30 microns. The resulting powder from placental tissues is subjected to lyophilization at temperatures in the range (-10 ° C) - (-20 ° C) and a residual pressure in the chamber of not higher than 0.1 mm Hg. within 16-20 hours. The result is a powder with a moisture content of 3-5%.

Extraction of lyophilized powder of porcine placenta is carried out on a low-temperature extraction unit using freon, which is absolutely inert to the extracted fraction. Liquid freon is supplied from pressure vessels at a pressure of 6-8 atm and a temperature of 30-35 ° C to the extractor, where the previously dried powder is located. The solvent passes through a layer of freeze-dried powder of porcine placenta and extracts fat-soluble substances from it, then it is poured through a fine filter into an evaporator. In the evaporator, due to a decrease in pressure to atmospheric, given the low boiling point of freons (-20) - (-40 ° C) and their volatility, it is possible to completely remove the solvent (it is sent for regeneration and reuse).

The isolation of the hydrophilic complex of porcine placenta is carried out by extraction of the skimmed powder of the residue after lipophilic extraction in isotonic sodium chloride solution at a temperature of 25-30 ° C for 3-4 hours with constant stirring. The extraction is repeated twice. The extract obtained is filtered and a hydrophilic fraction of porcine placenta is obtained. The solution is stored in a freezer at a temperature of -18 ° C.

The result is a preparation with the following ratio of components, wt.%:

Hydrophilic fraction of tissue of the placental pork 2-3

Isotonic sodium chloride solution

Optimum is the following ratio of components, wt.%:

Hydrophilic fraction of porcine placental tissue 2.5

Isotonic sodium chloride solution rest.

A decrease in the content of the hydrophilic fraction of porcine placenta in the preparation reduces the effect of the application, and an increase does not lead to an increase in the therapeutic effect.

The preparation contains a complex of biologically active substances: amino acids, peptides, nucleic acids, hexuronic acids, polysaccharides, vitamins, microelements. The drug has an immunostimulating effect, normalizes indicators of cellular and humoral immunity, has an anti-inflammatory effect, stimulates the enzyme system and normalizes the microbial composition of the digestive tract, has an adaptogenic and stress-protective effect, and increases the nonspecific resistance of the body.

The method is as follows.

Pre-heated to a temperature of + 36-37 ° C, the drug is administered intramuscularly at a dose of 0.01 ml / kg body weight or orally drunk with water at a dose of 0.1 ml / kg body weight according to the following scheme:

Table 1
The application scheme of the proposed method Type of creature Indications for use Application chart Cows endometritis 5-7 times with an interval of 48 hours Pigs endometritis prevention after farrowing twice with an interval of 48 hours Piglets endometritis treatment 4-5 times with an interval of 48 hours

Positive results obtained by testing the proposed method, indicate the prospects of its further research and implementation in production. The experience of studying the therapeutic effectiveness of the proposed method for endometritis in cows was carried out under the conditions of the Slobozhansky agro-industrial complex from January 17, 2003 to February 22, 2003. The test results were recorded in a special journal during the experimental period as patients were identified and treated animals.

The inventive method was tested in the treatment of cows and heifers with acute catarrhal endometritis. After breeding, as signs of disease were detected, one group of cows used the claimed method according to the following scheme: intramuscularly at a dose of 0.01 ml / kg body weight 7 times with an interval of 48 hours. The second group of experimental animals, the method was used orally at a dose of 0.1 ml / kg according to a similar pattern.

The data obtained were compared with the control group, the treatment of which was carried out with a tissue preparation PDE, which was administered subcutaneously at a dose of 20 ml / animal 7 times with an interval of 48 hours. The results of comparative effectiveness are presented in table 2.

table 2
The comparative effectiveness of the proposed method for catarrhal endometritis in cows Way Number of cows Recovered and fertilized within 90 days HEAD % The inventive method, orally 42 28 67.5 The inventive method, injection 38 27 70.0 PDE 31 19 61.3

From the data presented in table 2, it can be seen that the claimed method is effective for endometritis in cows, and its clinical effectiveness is comparable and even slightly exceeds the preparation of PDE.

For all experiments and production tests of the method, positive results and good reviews were obtained. The proposed method can be used both in large livestock complexes, and in small farms.

Claims (1)

A method for the prevention and treatment of endometritis in cows and sows, including the use of a preparation based on placental tissues, characterized in that a preparation based on a hydrophilic fraction of pig placenta and isotonic sodium chloride solution is used as such in the following ratio of components, wt.%: Hydrophilic tissue fraction pork placenta - 2-3, isotonic sodium chloride solution - the rest.