RU2233668C1 - Method for preparing preparation of phenol nature from vegetable raw - Google Patents

Abstract

FIELD: pharmaceutical and food industry.

SUBSTANCE: invention relates to a method for preparing the preparation of phenolic nature from vegetable raw. Peach leaves or flowers, or leaves and flowers or eyebright stems and/or leaves, and/or flowers, and/or roots are extracted with purified water in the ratio = (1:10)-(1:20) at 95-100 C for 1 h, not less. The prepared extract is poured off and to the remained mass 20-60% of water of its initial volume is added and extracted again under the same conditions. Extracts are combined, concentrated at heating at 95-100 C up to reducing volume of extract by 2-fold, not less. The concentrate is settled at 0-10 C for 24-48 h, filtered, centrifuged, unfiltered residue is separated and concentrated again by evaporation on boiling water bath up to preparing the concentrate with the content of phenolic substances 10%, not less, to the volume unit. During the extraction process inert substances from surface of extract are removed, the concentrate is settled for 24 h, not less, at 0-10 C, precipitate is removed and the final product is preserved with ethyl alcohol. Invention provides enhancing quality of the preparation, reducing inert substances and toxicity, elevating concentration of substances of phenolic nature, and increasing yield of useful product.

EFFECT: improved preparing method.

4 tbl, 2 ex

Description

The invention relates to methods for producing a preparation from plant materials and can be used in the pharmaceutical and food industries.

There is a method of obtaining a preparation of phenolic nature from plant materials comprising water extraction (Encyclopedia of traditional medicine. - M., ANS, 1998, p.227 and 228).

Its disadvantage is an insufficient concentration of phenolic substances and the presence of ballast substances.

Closest to the proposed solution is a method of obtaining a phenolic preparation of nature from plant materials, including water extraction, concentration and conservation (Copyright certificate. USSR No. 577033).

Its disadvantage is the low concentration of phenolic substances and the presence of ballast substances, the toxicity of the drug.

The invention solves the problem: improving the quality of the drug, reducing the amount of ballast substances, reducing toxicity, increasing the concentration of phenolic substances, increasing the yield of useful product, expanding the spectrum of action.

To achieve the technical result in the proposed method, leaves or flowers or leaves and peach flowers or stem, root, leaves and flowers or stem, leaves and eyebright flowers are extracted with purified water in a ratio of 1: 10-1: 20 at 95-100С ° not less 1 hour, the resulting extract is drained, 20-60% of the original volume is added to the remaining mass, re-extracted under the same conditions, the extracts are combined, concentrated by heating to 95-100 ° C to reduce the extract volume by at least 2 times, concentrate defend at 0-10 ° C for 24-48 hours, filtered, centrifuged, the unfiltered precipitate is separated, re-concentrated by evaporation in a boiling water bath to obtain a concentrate of at least 10%, during the concentration process, ballast substances are removed from the surface of the extract, the concentrate is settled for at least 24 hours 0-10C ° precipitate is separated, and the final product is preserved with ethanol.

Distinctive features of the proposed method are the following features: leaves or flowers or leaves and peach flowers or stem, leaves, root, flowers or stem, leaves and eyebright flowers are extracted with purified water in a ratio of 1: 10-1: 20 at 95-100С ° not less 1 hour, the resulting extract is drained, 20-60% of the original volume is added to the remaining mass, re-extracted under the same conditions, the extracts are combined, concentrated by heating to 95-100 ° C to reduce the extract volume by at least 2 times, concentrate uphold at 0–10 ° C for 24–48 hours, filter, centrifuge, the unfiltered precipitate is separated, re-concentrate by evaporation in a boiling water bath to obtain a concentrate of at least 10%, in the process of concentration, ballast substances are removed from the surface of the extract, the concentrate is settled for at least 24 hours at 0-10 ° C, the precipitate is separated, and the final product is preserved with ethanol. The proposed method allows to obtain a harmless drug with a wide spectrum of action. Animal studies have shown that it does not show toxicity, mutagenicity, embryotoxicity, allergenicity, teratogenicity. Studies have shown that the drug is an agent that exhibits antitumor, antioxidant, antiallergenic, adaptogenic, immunomodulatory activity in a wide range of doses.

The proposed method is implemented in the following sequence.

Plant raw materials are laid in a steam boiler, pour purified water in a ratio of 1: 10-1: 20 and extracted at a temperature of 95-100С ° for at least an hour, the resulting extract is poured, 20-60% of the initial volume of water is added to the boiler to the remaining mass and extraction is continued using the same technology. The resulting extract is mixed with the primary extract for subsequent concentration. The resulting extract is concentrated by heating to 95-100 ° C until the extract volume is reduced by at least 2 times, the concentrate is defended at a temperature of 0-10 ° C for at least 24 hours, filtered, an unfiltered precipitate containing ballast substances is separated by centrifuge, and then carried out secondary concentration by evaporation of a primary concentrate in a boiling water bath to obtain a concentrate with a content of phenolic substances in the unit volume of at least 10%. The determination of the amount of phenolic substances is carried out by a known method (see the State Pharmacopoeia of the USSR. - M .: Medicine, 1987, p. 286, 287).

Moreover, in the process of concentration, ballast substances (xanthophylls and chlorophylls) are removed from the surface of the extract, and the final product is further defended for at least a day at a temperature of 0-10 ° C to form a ballast substance precipitate, and then the precipitate is separated.

Preservation is carried out with a solution of ethyl alcohol, achieving a content of ethyl alcohol in the final product of at least 7%, and phenolic substances of at least 5%.

Example 1

Plant material leaves and (or) peach flowers are laid in a steam boiler, pour purified water at a ratio of 1:10 and extracted at 95 ° C for 3.5 hours, the resulting extract is poured, 60% of water is added to the remaining mass in the boiler initial volume and extraction are continued using the same technology. The resulting extract is mixed with the primary extract for subsequent concentration. The resulting extract is concentrated by heating to 100 ° C to reduce the volume of the extract by 3 times, the concentrate is left standing at 0 ° C for 48 hours, filtered and the unfiltered precipitate containing ballast substances is separated by centrifuge, and then secondary concentration is carried out by evaporation in boiling water bath of primary concentrate until a concentrate is obtained with a content of phenolic substances (coumarins, tannins, flavonoids, saponins, aromatic phenolic acid, chlorogenic, phenol glyco zides) at least 10%.

Moreover, in the process of concentration, ballast substances (xanthophylls and chlorophylls) are removed from the surface of the extract, and the final product is further defended for at least a day at a temperature of 0-10 ° C to form a ballast sediment, and then the precipitate is separated.

Preservation is carried out with a solution of ethyl alcohol, achieving a content of ethyl alcohol in the final product of at least 7%, and phenolic substances of at least 5%.

Example 2

Vegetable raw materials - eye-plant (stem, leaves, flowers and roots) are laid in a steam boiler, pour purified water at a ratio of 1:20 and extracted at 100 ° C for 1 hour, the resulting extract is poured, water is added to the boiler to the remaining mass 20 % of the initial volume and extraction is continued according to the same technology, the extract obtained after evaporation is mixed with the primary extract for subsequent concentration. The extract obtained is concentrated by heating the extract to 95 ° C until the volume is reduced by 2 times, the concentrate is left standing at 10 ° C for 24 hours and filtered, and the unfiltered precipitate is separated by centrifuge, and then the secondary concentration is carried out by evaporation of the primary concentrate in a boiling water bath to obtaining a concentrate with a content of phenolic substances per unit volume of 10% (flavonoids - 13 substances, including cinaroside, luteolin, apigenin; phenol carboxylic acids - 14 acids, including chlorogenic and coffee; iridoids - 7 substances, including compounds of the catalpol subgroup, aucubin, odontoside, tannins, coumarins, carotenoids).

Moreover, in the process of concentration, ballast substances are removed from the surface of the extract, and the final product is further defended for at least a day at a temperature of 0-10 ° C, and then the precipitate is separated.

Preservation is carried out with a solution of ethyl alcohol, achieving a content of ethyl alcohol in the final product of at least 7%, and phenolic substances of at least 5%.

The proposed method allows to obtain a harmless drug with a wide spectrum of action. Animal studies have shown that it does not show toxicity, mutagenicity, embryotoxicity, allergenicity, teratogenicity.

Studies have shown that the drug is an agent that exhibits antitumor, antioxidant, anti-allergenic, anti-inflammatory adaptogenic, immunomodulatory activity in a wide range of doses.

Studies have shown that a herbal preparation of a polyphenolic nature has a pronounced ability to have a stimulating effect on all parts of the immune system: phagocytosis, cellular and humoral immune responses (Table 1-3).

From table 1 it is seen that the drug significantly increases the titer of antibodies in a wide range of doses.

From tables 2 and 3 it is seen that the drug in a wide range of doses stimulates the processes of phagocytosis of pathogenic agents. The drug has the ability to protect the immune system from the damaging effects of chemical agents (table. 4).

From table 4 it is seen that CF almost 3 times reduces the number of antibody-forming cells compared to the control. The introduction of the drug before exposure to CF almost 100% protects the cells of the immune system.

Clinical trials of the drug with the participation of a group of cancer patients showed that taking the drug reduces cancer intoxication (weakness, fatigue, nausea, vomiting, fever, pain), increases the working capacity and non-specific resistance of the body, helps to normalize sleep, improves the quality of life of patients.

“A clinical evaluation of the drug with the participation of gynecological patients showed that this drug is an effective tool.

The use of the drug for 3 months contributes to:

- regression to normal or “slightly larger than normal” size of the uterus with a diagnosis of uterine fibroids and adenomyosis;

- reduction or disappearance of signs of fibrocystic mastopathy;

- the disappearance or reduction of pain in the pelvic area, spotting discharge before and after menstruation;

- normalization of blood loss during menstruation and an increase in hemoglobin to normal with its initially reduced level.

The use of the drug in children led to a significant increase in the parameters of the immune system, in particular phagocytic activity and indicators of red blood, especially hemoglobin. It can be recommended for children under 12 years of age as a general strengthening agent in the prevention and treatment of diseases accompanied by a decrease in the activity of the immune system and with allergies.

The drug was tested in the treatment of pulmonary tuberculosis, eye diseases and chemotherapy in cancer patients, which showed its effectiveness.

Allergic and other adverse reactions during the tests were not identified.

Thus, the proposed method of obtaining a phenolic preparation of a nature from plant materials provides a reduction in the amount of ballast substances, a decrease in the toxicity of an increase in the concentration of phenolic substances, an increase in the yield of a useful product, and an extension of the spectrum of action.

Claims (1)

A method of obtaining a phenolic preparation of nature from plant materials, characterized in that the leaves or flowers or leaves and peach flowers, or the stem, and / or leaves, and / or flowers, and / or eyebright roots are extracted with purified water in a ratio of 1: 10-1 : 20 at 95-100 ° C for at least 1 h, the resulting extract is drained, 20-60% of the original volume is added to the remaining mass, re-extracted under the same conditions, the extracts are combined, concentrated by heating to 95-100 ° C to reducing the volume of the extract by at least 2 times, I uphold the concentrate t at 0-10 ° C for 24-48 hours, filtered, centrifuged, the unfiltered precipitate is separated, re-concentrated by evaporation in a boiling water bath to obtain a concentrate with a content of phenolic substances in the volume unit of at least 10%; during the concentration, ballast substances are removed from the surface of the extract, the concentrate is defended for at least a day at 0-10 ° C, the precipitate is separated, and the final product is preserved with ethyl alcohol.