RU2181595C2 - Method to treat acute endometritis in cows - Google Patents

Abstract

FIELD: veterinary science. SUBSTANCE: cows are subjected to intrauterine injection of ozonized fish oil at 25-50 ml dosage at 48-h-long interval. EFFECT: shortened period of infertility and increased coefficient of animal impregnation. 3 tbl

Description

 The invention relates to veterinary medicine, namely to veterinary gynecology, and can be used to treat cows with acute endometritis.

 Known methods, methods and schemes [1, 2] for the treatment of endometritis: etiotropic and novocaine therapies, physiotherapy, hormonal and vitamin preparations, disinfectant solutions, mud, autohemo-and tissue therapy and others. The basis of the complex therapy of animals with inflammatory processes in the genitals is antimicrobial drugs as a means of etiotropic therapy aimed at suppressing the pathogen. As antimicrobial agents, antibiotics, sulfonamides, nitrofurans and other chemotherapeutic and antiseptic drugs are used.

 The disadvantages of the etiotropic method of treating endometritis include the fact that it is not effective due to the high resistance of microorganisms, and the antibiotics and sulfonamide drugs, nitrofurans, which are part of the drugs, negatively affect the factors of non-specific resistance of the body and the morphofunctional state of the mucous membrane of the reproductive apparatus.

 A known method [3] of etiotropic therapy of cows with endometritis, which is based on the intrauterine administration of a protein of a chicken egg with 1.0 furacilin, 0.5 furazolidone and 3.5 streptocide dissolved in it. Additionally, 900-1000 ml of oxygen in the form of foam is injected into the uterine cavity. Fertilization of cows after clinical recovery is 85.2% with a fertilization index of 1.5.

 The disadvantage of this method of treatment is primarily its high cost, lack of therapeutic effectiveness, the need for rejection of milk during the course of treatment, and also within 2 days after the disappearance of the clinical signs of the disease.

 The essence of the proposed method lies in the fact that for the treatment of acute endometritis in cows, a common symptom similar to the prototype is proposed - etiotropic therapy. In relation to the prototype, the distinguishing features are: the use of ozonated fish oil (ORF), which is then used by intrauterine devices to treat cows with endometritis until their clinical recovery with an interval of 48 hours.

Preparation of ozonated fish oil consists in passing ozone through it at a concentration of 10 mg / l for 60 minutes. Previously, fish oil is poured into clean glass bottles with a capacity of 500 ml in an amount of 400 ml, closed with a black rubber stopper and two needles of various lengths are inserted. A needle 12 cm long and 1 mm in diameter is connected to the ozonator hose and ozone is passed through it. Through another needle (4 cm long and 1 mm in diameter), exhaust gas is discharged into the device’s destructor using a polyurethane hose. The resulting preparation is checked for sterility, irritation, toxicity and antimicrobial activity according to generally accepted methods. ORM is administered intrauterine at a dose of 25-50 ml (37-38 ^o C), starting with the onset of clinical signs of endometritis with an interval of 48 hours until the recovery of sick cows.

 To determine the optimal single dose, MRE was instilled into the uterine cavity with a rectocervical method in doses of 20, 25, 50, 75 ml. A positive therapeutic effect was obtained when a dose of 25-50 ml was used, and therefore the indicated dosage was taken as a basis.

 In terms of blood received from cows after their clinical recovery, the treatment of which was performed by ORM, an increase in the number of erythrocytes by 9.6%, stab neutrophils by 29.9%, total protein by 11.9% was observed compared with the initial level but there was an increase in alkaline reserve by 13.1%. bactericidal and lysozyme activity of blood serum by 2.0% and 25.4%, respectively. At the same time, there was a decrease in the total number of leukocytes by 9.6% and lymphocytes by 19.7%, as well as globulins by 19%.

To determine the antibacterial activity of ORM, uterine contents were obtained from 10 cows with acute endometritis. A bacteriological study included the seeding of microbes from exudate samples by the method of serial dilutions on BCH. IPA and Wednesday Kitt-Tarozzi. Crops were cultivated at t 37 ± 1 ^o C for 20 hours. As a result of the study, it was found that the exudate samples contained a microbial population represented by cultures of Staphylococcus aureus (72 ± 18%) and gram-negative bacilli (28 ± 18%). The number of microbial cells ranged from (5.6 ± 0.2) x10 ⁴ to (3.2 ± 0.6) x10 ⁶ .

Evaluation of the antimicrobial activity of PCM in relation to the culture of the microbial population isolated from exudate samples was carried out by adding 0.1 ml of the microbial suspension containing 1 • 10 ⁶ mt / ml according to the optical concentration (OK) of the daily agar culture in 1 ml tubes ozonated fish oil. Samples with unzoned fish oil were used as a control.

The experimental and control samples were kept at t - 37 ± 1 ^o C for 1 h, after which the appropriate ten-fold dilutions were plated on Petri dishes with MPA. Crops were incubated for 20 hours at a temperature of 37 ± 1 ^o C.

Subsequently, the maximum microbial load was determined, in relation to which the PCF had antibacterial activity. For this, 0.1 ml of a microbial suspension containing 10 ¹⁰ to 10 ⁶ bwt / ml OK was added to the tubes with 1 ml of ORF (OK) (dilution step 10) (Table 1).

 As a result of the experiment, it was found that MRE has a pronounced antimicrobial activity against microbial cultures isolated from the exudate of the uterus of cows with endometritis.

Subsequently, the dynamics of inactivation of the microbial culture isolated from the uterine exudate was assessed. To do this, 0.1 ml of a microbial suspension containing 1x10 ⁷ bw / ml in OK was added to the tubes with 1 ml of ORF. Samples were incubated at t -37 ± 1 ^o C for 0, 10, 20, 30, 40, 50 and 60 min, respectively. At the indicated time intervals, seeding was performed from samples on MPA to determine CFU (Table 2).

 Based on the studies, we can conclude that the complete inactivation of microbial cells in the PCM occurs 30 minutes after their introduction.

 In order to determine the effectiveness of the use of PCM in the treatment of cows with acute postpartum purulent-catarrhal endometritis, the experimental and control groups of animals were selected according to the principle of paired analogues. The cows of the experimental group (n = 20) were treated with ORM at a dose of 25-50 ml for one intrauterine injection with an interval of 46 hours before clinical recovery. The animals of the control group (n = 20) were treated with a 10% suspension of tricillin in fish oil at a dose of 100 ml for a single injection with the same interval. The effectiveness of the treatment was determined by the number of recovered cows, drug injections, days of infertility and fertilization rate (table. 3).

 It was found that the average number of treatment procedures required for the clinical recovery of the cows of the experimental group averaged 4.2, which is 0.8 times more than in the control group. However, the fertilization rate in the experimental group of cows averaged 1.56, while for the fertilization of animals in the control group an average of 2.06 inseminations were required (P <0.2). The number of days of infertility in the experimental group of cows averaged 46.9 days, which is 26.8 days less than the identical indicator for cows in the control group.

 Based on the above data, we can conclude that the therapeutic efficacy of PCM is higher in comparison with the generally accepted method for the treatment of cows with endometritis, involving the use of a 10% suspension of tricillin.

Literature
1. Goncharov V.P., Karpov V.A. Prevention and treatment of gynecological diseases of cows. M .: Rosagropromizdat, 1991 .-- S. 92.

 2. Grigoryeva T. E. Treatment and prevention of endometritis in cows. M .: Rosagropromizdat, 1988 .-- S. 22.

 3. Khilkevich N.M. Prevention and treatment of mastitis // Veterinary medicine. -1987. - 4. - S. 53. - prototype.

Claims (1)

 A method of treating acute endometritis in cows, including the intrauterine administration of an antimicrobial preparation, characterized in that ozonated fish oil is administered as an antimicrobial preparation in a dose of 25-50 ml with an interval of 48 hours.

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