RU2151427C1 - Method for modeling acute peritonitis - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: method involves introducing catheters through puncture holes in the anterior abdominal cavity wall. The catheters are set in the right and left subdiaphragmatic spaces, right and left lateral abdominal canals, small pelvis and the central part of the abdominal cavity. Autoblood is introduced through the catheters into the abdominal cavity at a dose of 7-10 ml per 1 kg of body mass. Microbe suspension composed of staphylococci, Escherichia coli, blue pus bacilli and peptococci is introduced in three stages in equal proportions 24 h later. Microbe bodies suspension is introduced at the first stage in the amount of 50-55 billions of microbe bodies per 1 kg of animal body mass, and 20-25 billions of microbe bodies per 1 kg of animal body mass are introduced at the second and the third stage. The suspension is administered in holding 6 h pauses. EFFECT: enhanced accuracy in approximating real clinical picture of pathological process dynamics.

Description

 The invention relates to medicine, namely to experimental surgery.

 A prototype should be considered a method of modeling acute peritonitis, consisting in a puncture simultaneous intraperitoneal injection of fecal suspension / C.A. Shalimov, A.P. Radzikhovsky, L.V. Keisevich "Guide to experimental surgery." -M.: Medicine, p. 125 /.

 The disadvantages of this method include the following - the possibility of unexpected injury to the abdominal cavity, the difficulty of obtaining the same type of dynamics of the pathological process, the death of some laboratory animals from toxic-septic shock.

 The aim of the present invention was to develop a new method for modeling acute peritonitis, which allows to obtain the dynamics of the development of the inflammatory process in the abdominal cavity, as close as possible to clinical manifestations, as well as making it possible to obtain pathological reactions of the same type and nature, which increases the reliability of the data obtained.

 This goal is achieved by introducing into the abdominal cavity a combined suspension of microbial bodies represented by staphylococci, Escherichia coli, blue-green pus bacillus and peptococci in equal proportions, which is distributed over all parts of the abdominal cavity using catheters previously drawn through puncture holes in the anterior abdominal wall, established in the right and left subphrenic spaces, the right and left side channels of the abdomen, the pelvis and the central part of the abdominal cavity, while A suspension of microbial bodies is injected 24 hours after preliminary introduction into the abdominal cavity through the above catheters of autologous blood in a total volume of 7-10 ml / kg body weight of an experimental animal in three stages, while at the first stage, a suspension of microbial bodies is introduced in the amount of 50-55 billion microbial bodies / kg of animal body weight, in the second and third - 20-25 billion microbial bodies / kg at six-hour intervals.

 The proposed method can be used to simulate a purulent-inflammatory process in the abdominal cavity in various laboratory animals / dogs, rabbits, etc. /.

 An example of modeling acute widespread peritonitis in a mongrel dog / dog No. 3, weight 19 kg, male /. At the first stage, the animal was sampled blood, which was subsequently introduced into the abdominal cavity in the amount of 7-10 ml / kg of body weight. The next stage of peritonitis modeling was puncture of the abdominal wall (under anesthesia) and the introduction of 6 thin silicone catheters into the abdominal cavity. Each catheter was placed in a strictly defined place in the abdominal cavity - in the right lateral canal, left lateral canal, right subphrenic space, left subphrenic space, small pelvis, center of the abdominal cavity, through separate punctures. After installation in the abdominal cavity, the ends of the catheters were fixed to the aponeurosis and removed either under the skin or under the bandage, which excludes spontaneous removal of the catheters. An equal amount of autoblood was injected into each catheter in a total volume of 160 ml. After this, after 24 hours, a polymicrobial suspension was introduced into the abdominal cavity, consisting of staphylococci, Escherichia coli, blue-green pus bacillus and peptococcus in equal proportions. In order to prevent the development of toxic-septic shock and premature death of the animal, the microbial suspension was introduced fractionally. The volume of polymicrobial suspension introduced at the first stage amounted to 55 billion microbial bodies / kg of animal body weight. The second and third injections of polymicrobial suspension were performed at six-hour intervals in the amount of 25 billion microbial bodies / kg of animal body weight. The same amount of solution with polymicrobial suspension was injected into each catheter. After the last stage of bacterial contamination of the abdominal cavity with a suspension of microbial bodies was completed, the catheters were left in the abdominal cavity until the initial operation was completed for peritonitis. This made it possible to confirm, after laparotomy and revision of the abdominal organs, the correct placement of catheters and the compliance of bacterial contamination of the corresponding parts of the abdominal cavity with the requirements.

 The development and study of the proposed method for modeling acute peritonitis was carried out on 40 outbred dogs. When using the proposed model, 36 hours after the bacterial contamination of the abdominal cavity in experimental animals, a clear clinic of the toxic stage of acute widespread peritonitis develops. If untreated, mortality is 90-95%.

 The use of this model of acute widespread peritonitis makes it possible to significantly approximate the dynamics of the pathological process to real clinical conditions, to simulate a purulent-inflammatory process with the same localization, which together increases the reliability of experimental studies.

Claims (1)

 A method for modeling acute peritonitis, which consists in puncture introduction of pathogenic microflora into the abdominal cavity, characterized in that bacterial contamination of the abdominal cavity is carried out by a combined suspension of microbial bodies represented by staphylococci, E. coli, blue-green pus bacillus and peptococcus in equal proportions, which is distributed over all sections of the abdominal cavity using previously conducted through the puncture holes in the anterior abdominal wall of catheters installed in the right and the left subphrenic spaces, the right and left lateral channels - the abdomen, pelvis, and the central part of the abdominal cavity, while the indicated suspension of microbial bodies is introduced 24 hours after preliminary introduction of autologous blood into the abdominal cavity through these catheters in a total volume of 7 - 10 ml / kg the body weight of the experimental animal in three stages, while in the first stage, a suspension of microbial bodies is introduced in the amount of 50 - 55 billion microbial bodies / kg of body weight of the animal, in the second and third - 20 - 25 billion microbial bodies / kg after 6- hourly interval .