RU2129003C1 - Agent showing antitumor activity - Google Patents

Abstract

FIELD: medicine, oncology. SUBSTANCE: invention relates to agents of plant origin showing antitumor activity. An antitumor agent has fungus Reishi, fungus kavaratake and ginseng roots taken at ratio 10:5:3, respectively. EFFECT: high antitumor activity, low toxicity of an agent. 1 cl, 2 ex

Description

The invention relates to medicine, namely to Oncology, and relates to means of plant origin with antitumor activity
Among the herbal preparations used in oncological practice, it should be noted first of all the alkaloids of the genus Colchium — colchicine and colchamine isolated from magnificent colchicum [4]. However, their toxicity is quite high and, when used, serious complications may develop - leukopenia, thrombocytopenia [1,2]. The alkaloid of the periwinkle plant - rosewin (vinblastine) is used for hematosarcoma and lymphogranulomatosis. It significantly inhibits leukemia and thrombopoiesis, causes weakness, loss of appetite, vomiting, jaundice [3].

However, less toxic drugs have less pronounced antitumor activity. So it is known to use an aqueous extract of mistletoe white as an antitumor drug in breast cancer. At the same time, the effectiveness of the drug is low (5)
The essence of the invention lies in the fact that for the treatment of tumors, a composition is proposed consisting of Reishi, Kavaratake and ginseng in a ratio of 10: 5: 3.

 To obtain funds, the material is ground to particles of 1-2 cubic meters. mm The mixture is poured with distilled water in an amount of 500 ml. It is refluxed for 3 hours, filtered off and thermostated for one day.

 Below is a brief description of the components that make up the composition.

 Reishi mushroom (Ganoderma lucidum) is a representative of the genus fungi basidiomycetes (Basidiomycetes). By nature - a typical xylotroph living on wood. The mushroom has a shiny, waxy, as if varnished hat and leg. The length of the stalk reaches 15 cm. The color of the fruit body of the fungus in the process of growth varies from orange-yellow to brown-red and wine-red with aging. The mushroom grows on stumps and at the bases of sick, weakened trees, forms white rot (6). The studies were carried out with a brown-red colored mushroom (collected in the forests of northern China during the summer period, mature, but not old). Fruit bodies are dried at room temperature, in the shade, when aired.

 Mushroom Kavaratake (Corios Verficolor) is a representative of the class of basidiomycetes. Grows in Western Japan (Nagano, Shikoku, Kuyushu). By its nature, it is a xylotroph living on deciduous trees. The experiments used fruiting bodies collected in the summer, dried at room temperature, in the shade, and when aired.

 Ginseng (Angerica Shikokiana Makino) Mountain carrots. It grows in the mountainous regions of western Japan (Nagano, Shikoku, Kuyushu). In the experiments, the roots of an adult plant collected in the summer period, dried at room temperature, in the shade and during ventilation were used.

 To disclose the invention below are the results of experimental studies of the claimed composition.

1. The effect of the claimed funds on the virus - induced erythroblastic leukemia Rausher mice males of the BALB / c line
In the experiment, 40 males were used - mice weighing 22-24 g. On day 0, 0.2 ml of virus-containing plasma at a dilution of 1:50 was introduced to all mice. Therapy started on day 11. At the time of initiation of therapy, splenomegaly 1+.

 The therapy was carried out by daily oral administration of the claimed drug in doses of 63, 39, and 12 ml / kg per day for 37 days, i.e. up to 47 days of experience. Splenomegaly developed at the same rate in all groups and reached 4+ by the 23rd day of the experiment, i.e. after 13 days of therapy.

 The table shows the effect of the claimed funds on the survival of mice with advanced erythroblastic leukemia.

 Thus, from the presented data it can be seen that the claimed agent exhibits antitumor activity in doses below 40 ml / kg. It should be noted that mice appear in these groups, which, despite the pronounced splenomegaly, significantly survived the growth control. The maximum life expectancy in the control is 57 days, in the experimental groups - 75-120 days (Fig. 1 and 2).

 It is known that one day of a mouse’s life is equal to 35 days of a person’s life [7].

2. The effect of the claimed funds on the growth of ascites tumor T-lymphoma P388 in male mice of line C
We studied 5 groups of mice, 10-12 animals per group (54 mice). Control - 20 mice.

In the control group, from 8 to 19 days of the experiment, 45% of the animals fell (Fig. 3). In the experimental groups, the studied drug was administered in the following mode:
1) 0.75 ml / kg / d (2% 0.3 ml per administration)
2) 1.5 ml / kg / d (4%, 0.3 ml per administration)
3) 7.5 ml / kg / d (20% - 0.3 ml per administration)
4) 37.5 ml / kg / d (100% - 0.3 ml per administration)
5) 87.5 ml / kg / d (100% -0.7 ml per administration).

 The extract was administered from 4 to 27 days (24-day course). Resorption of ascites was observed in all 54 mice. No deaths were observed, up to 90 days of the experiment. Thus, in this series of experiments, it was shown that the use of the claimed agent led to the complete cure of mice with ascites P388 lymphoma.

 The invention is illustrated by the following examples.

 Example 1

 In the experiment, 10 males were used - mice weighing 22-24 g. On day 0, 0.2 ml of virus-containing plasma at a dilution of 1:50 was introduced to all mice. Therapy started on day 11. At the time of initiation of therapy, splenomegaly 1+.

 The therapy was carried out by daily oral administration of the claimed drug in doses of 39 ml / kg per day for 37 days, i.e. up to 47 days of experience.

 To obtain an antitumor agent, take 10 g of Reishi mushroom, 5 g of Kavaratake mushroom and 3 g of ginseng. The material is crushed to particles of 1-2 cubic mm. The mixture is poured with distilled water in an amount of 500 ml. It is refluxed for 3 hours, filtered off and thermostated for one day.

 Splenomegaly developed at the same rate in all groups and reached 4+ by the 23rd day of the experiment, i.e. after 13 days of therapy.

 With the introduction of the drug at a dose of 39 ml / kg, the average life expectancy was 45.4 days, an increase in average life expectancy compared with the control of 22%.

 Example 2

 12 mice were examined. Control - 5 mice.

 In the control group, from 8 to 19 days of the experiment, 45% of the animals fell. In the experimental group, the studied drug was administered at a dose of 37.5 ml / kg / d (100% -0.3 ml per administration).

 To obtain an antitumor agent, take 10 g of Reishi mushroom, 5 g of Kavaratake mushroom and 3 g of ginseng. The material is crushed to particles of 1-2 cubic mm. The mixture is poured with distilled water in an amount of 500 ml. It is refluxed for 3 hours, filtered off and thermostated for one day.

 The extract was administered from 4 to 27 days (24-day course). Resorption of ascites was observed in all mice. No deaths were observed, up to 90 days of the experiment.

 Thus, in the presented materials it was shown that the claimed substance has reliable antitumor activity at the same time, in experiments it was shown that the toxicity of the drug is low. When using the claimed agent in doses above 60 ml / kg, a significant decrease in animal body weight was noted (more than 20%), the appearance of alopecia by the third month of the experiment, the claimed means of animal death used in all studied doses did not cause in any case.

List of references
1. Belitsky K.P. Medicinal plants and cancer. Kiev, 1982

 2. Vermel EM. Comparative evaluation of close-focus radiography in the treatment of skin cancer with Omain. Chemistry and Medicine 1956, pp. 73-80.

 Z. Mashkovsky M.D. Medicines, 1985, t 2, S. 464-466.

 4. Mid P.M. Medicinal plants and their use. Stavropol, 1973

5. Kraft D. Munh Med Wchshr. 1980, 87. P 1305-1307
6. Kohda H. Chem / Pharm Bull, 1985.33, N 4, p. 1367
7. Th Bohm at all. Nature 1997, 27 Now, p. 404-407z

Claims (1)

 An agent with antitumor activity, including herbal components, characterized in that it contains Reishi mushroom (Ganoderma Lucidum), Kavaratake mushroom (Corios Vevficdov) and Ginseng root (Angerica Shikokiana Hakino) in a ratio of 10: 5: 3.

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