JP4245291B2 - Bioactive composition and method for producing the same - Google Patents

Description

Ganoderma lipsienseの細胞増殖抑制率
【表２】

Tramates hirsutusの細胞増殖抑制率
【表３】

Panax japanicus C.A.Meyer）の細胞増殖抑制率
【表４】

Ganoderma lucidumの細胞増殖抑制率
【表５】

Tramates versiclolorの細胞増殖抑制率
【表６】

【００５９】
これらの結果に示すように、実施例試料のマンネンタケ（表１及び２）は、いずれも対照試料のマンネンタケ(表５)とおおよそ同等あるいはそれ以上の細胞増殖抑制率を示していた。特に、７２時間及び９６時間の培養時間において安定しかつ高い細胞増殖率を示していた。
また、実施例試料のカワラタケ（表３）は、対照試料のカワラタケ（表６）とほぼ同等の細胞増殖抑制率を示していた。
これらのことから、実施例試料のマンネンタケ及びカワラタケの熱水抽出液には、単独で抗腫瘍活性があることが明確であった。
また、チクセツニンジンについても、実施例のマンネンタケ、カワラタケ、対照試料の担子菌と同等かそれ以上の細胞増殖抑制率を有していた。すなわち、チクセツニンジンは、単独で坑腫瘍活性があることが明かであった。
したがって、これらの特定種のマンネンタケおよび／またはカワラタケおよび／またはチクセツニンジンを有効成分として含有する組成物を抗腫瘍活性のある治療あるいは予防剤として使用できることがわかった。また、これらの担子菌類の他、ウコギ科植物の根（典型的にはチクセツニンジンなどの薬用ニンジン）を有効成分として含有する組成物を、含む組成物などを、抗腫瘍活性のある治療あるいは予防剤として使用できることがわかった。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a composition having physiological activity such as antitumor activity. In particular, fungi belonging to the family Basidiomycete Sarnocosidaceae, in particular, fruit bodies and / or mycelial cultures (including other cultures of mycelium) of root genus of the genus Amanita and Kawaratake, and the roots of Argiaceae as active ingredients Relates to the composition.
[0002]
[Prior art]
Conventionally, the fruiting body of the mantis bamboo (commonly known as Reishi) belonging to the family Basidiomycete moss, and mycelium culture (including mycelium and culture solution; hereinafter simply referred to as culture) are herbal medicines for a long time. It is known as having various medicinal effects. Similarly, various physiologically active substances have also been extracted from the fruiting bodies of Agaricus belonging to the family Basidiomycete Sarnocosidaceae.
[0003]
In addition, the roots of plants belonging to the family Uglyceae including carrots and their extracted components have long been used as herbal medicines, and many of these are known to have a medicinal effect.
In general, various therapies including treatment and prevention with herbal medicines have a merit that side effects are small, but the effect may not be remarkable.
Although basidiomycetes and carrots are empirically and experimentally known to have excellent medicinal properties, their physiological activities are synergistic by combining these specific components and combining these components. It was not known to improve.
[0004]
[Means for Solving the Problems]
The present invention is provided by finding that a particular basidiomycete has a significantly high antitumor activity.
[0005]
Based on the above findings, the present invention provides the following compositions.
(1) A physiologically active composition having an antitumor activity containing, as an active ingredient, one or more selected from the group consisting of Ganoderuma pheiferi, Ganoderuma lipsense, Trametes hirstus, and Japan Panax ginseng.
(2) containing at least one kind of basidiomycete belonging to the family Basidiomycetes, and roots of plants belonging to the family Arcoceae, as active ingredients,
The basidiomycete is a physiologically active composition having antitumor activity, wherein the basidiomycete is one or more selected from the group consisting of Ganoderuma pheiferi, Ganoderuma lipsense, and Trametes hirsutus.
(3) The composition according to (2), wherein the root of the family Araceae is one or more selected from the group consisting of ginseng, chixenjinjin, and densithinjin.
(4) A physiologically active composition in which a raw material containing one or more selected from the group consisting of Ganoderuma pheiferi, Ganoderuma lipsense, Trametes hirstus and Japan Panax ginseng is extracted with water or a mixture of water and alcohol. Production method.
[0006]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described in detail.
The composition of the present invention is obtained from any one or more of the fruiting bodies and / or mycelium cultures of a specific species belonging to the genus Mushroom and Agaricaceae belonging to the family Basidiomycetes and the roots of the specific species of the family Araceae. The extracted component obtained is contained as an active ingredient. In addition to any fruiting body and / or mycelium culture of any of the specific species belonging to the family Basidiomycete Sarnocosidaceae and the specific species belonging to Kawaratake, it also contains the roots of Argiaceae plants or extract components obtained therefrom as active ingredients.
[0007]
(Basidiomycetes-derived active ingredient)
Ganoderuma pheiferi and Ganoderuma lipsense can be used as the basidiomycete mannentake. Either one or both can be used. Although the use of other mushrooms is not excluded, it is preferable to mainly contain these mushrooms. In the present specification, the taxonomic identification of basidiomycetes used is based on “Primary Color Japanese Fungi Encyclopedia” (Nuryosha Co., Ltd.) co-authored by Rokuya Imazeki and Tsuguo Hongo.
[0008]
Another example of the mushroom is Reishi Fungus (Ganoderma Lucidum). Although this fungus naturally thrives on trees, it is rare. Artificial cultivation is also possible. This bacterium has a glossy, wax-like bulk portion and a shaft portion, and the length of the shaft reaches about 15 cm. The fruit body colors are red, blue, yellow, white, purple, and black. This fungus grows on stumps and near the base of trees that are weakened by disease, forming white filaments.
[0009]
Trametes hirsutus can be used as the basidiomycete Kawaratake. Although it does not exclude the use of other oyster mushrooms, it is preferable to mainly contain this moth mushroom. In addition, Corriolus Versicolor can be illustrated as another mushroom. This fungus grows naturally in the western part of Japan, especially in the Shinshu region (especially Nagano Prefecture), Shikoku region, and Kyushu region. This fungus is naturally a good fungus, and particularly prefers hardwood.
[0010]
The basidiomycetes may be naturally occurring, may be artificially cultivated, or may be cell culture, and are not particularly limited. Preferably, it is naturally occurring.
[0011]
The form of these fungi used in the present invention may be fruit bodies and / or cell cultures, but is preferably fruit bodies. The fruit body is preferably air-dried by avoiding light at room temperature.
In particular, it is preferable to use a natural and mature black fruit body in the garlic mushroom. In the case of Kawaratake, it is preferably a native fruit body collected in summer, and more preferably air-dried avoiding light at room temperature.
In addition, the fruiting body can be processed into a dried solid substance, an alcohol immersion liquid, a paste or an extract, in addition to those not particularly processed after collection, and can be obtained in these forms.
[0012]
In the composition of the present invention, one kind or two or more kinds of the above-mentioned specific types of banyan mushrooms and kawatake mushrooms can be used. Preferably, both mannentake mushrooms and kawaratake mushrooms are used. It is particularly preferable to use only specific types of garlic mushrooms and kawaratake mushrooms.
[0013]
The composition of the present invention only needs to contain basidiomycetes as an active ingredient. Therefore, in the case of a fruit body raw material, the fruit body may be contained as it is as a solid active ingredient such as powder or strip.
In the case of a culture, it may be contained as a liquid such as a culture solution containing mycelial cells, or as a solid such as a dried product of mycelial cells.
[0014]
Moreover, the composition of this invention can contain the extract component obtained by extracting the fruit body of a basidiomycete or its culture using water and / or alcohol as an active ingredient.
[0015]
(Method for preparing basidiomycete extract components)
At the time of extraction, it is preferable to pulverize the fruiting bodies of basidiomycetes to form powders or strips. More preferably, it is in the form of fragments of about 7 mm square or less, and more preferably in the form of strips of about 4 mm to about 6 mm square. Most preferably, it is in the form of a strip of about 5 mm. A strip shape of 0.5 mm to 2 mm square may be preferable. When using mycelium cells as a raw material, it is preferable to use a dry powder.
[0016]
The basidiomycete active ingredient is preferably extracted from the above basidiomycete-derived raw material by a water: alcohol mixture in addition to water.
When extracting with water, the water to be used is not particularly limited, but it is preferable to heat the extraction. Preferably, it is about 80-100 degreeC, More preferably, it is about 90-95 degreeC.
The amount of water relative to the extracted raw material is not particularly limited, but is about 5 to about 25 parts by weight (more preferably about 10 to about 20 parts by weight, most preferably about 20 parts by weight) of the dried raw material. Thus, it is preferable to set the amount of water for extraction so that the amount of water after extraction is about 400 to 600 parts by weight. This is because when the final extract is prepared in this concentration range, it is a concentration preferable for oral administration as it is, and effective extraction is performed. More preferably, it is about 500 parts by weight.
[0017]
When the heat extraction operation is performed in a state that allows a reduction due to the evaporation of moisture, the initial amount of extraction water is set assuming a reduction in moisture during that time. For example, the amount of water is extracted by increasing it by about 50 to 60% with respect to the final amount of water. On the other hand, when the heat extraction operation is performed in a state where a reduction due to evaporation of moisture is not allowed, the extraction water amount to be finally obtained may be added to the raw material from the beginning and heated. Preferably, a reflux condenser is used.
[0018]
For the heating extraction operation, an open-air container or a reflux condenser can be used. In particular, in order to inhibit the extraction of the active ingredient and maintain the effectiveness of the extracted ingredient, it is preferable to use a material obtained by painting or processing a metal part with a corrosion-resistant coating, in addition to glass, enamel, or ceramic.
[0019]
Although the extraction time is not particularly limited, it is preferably at least 1 hour, more preferably 2 hours or more, and further preferably 2.5 hours or more. The upper limit is 3 to 4 hours.
[0020]
The following operation can be mentioned as a typical example in the case of extracting a basidiomycete raw material with water.
About 800 g of hot water is added to about 20 g of the dried basidiomycetous raw material (preferably fruit bodies, preferably in the form of strips), and the remaining amount of the extract remains while maintaining at 90 to 95 ° C. Roast to 500ml. Preferably, heating adjustment is performed so that the amount becomes 500 ml in about 3 hours. Alternatively, 500 ml of water is added to about 20 g and boiled for about 2 hours using a reflux condenser. The extract obtained by such an operation becomes a liquid having a concentration suitable for oral administration as it is.
[0021]
In the case of extracting with alcohol, it is preferable that the alcohol to be used is a drinkable alcohol in consideration of the case where it may be drunk as it is. That is, it is preferable to use ethanol.
When extracting using alcohol, you may extract only with alcohol, However, It is preferable to mix with water and to extract with a liquid mixture. The alcohol concentration is preferably 50 v / v%, more preferably about 35 v / v%.
The alcohol-containing extract is not particularly limited, but is preferably heated during extraction. Preferably, it is 50-80 degreeC, More preferably, it is 75 degreeC or less, More preferably, it is 70 degreeC or less.
Although the extraction time is not particularly limited, it is preferably 10 hours or longer, more preferably 20 hours or longer, and most preferably about 30 hours or longer.
It should be noted that the alcohol concentration is preferably about 20 v / v% at the final oral administration. Therefore, when extraction is performed with an alcohol solution having a concentration exceeding 20 v / v%, it is preferable to dilute with water after extraction so as to be 20 v / v%.
Further, the amount of the extraction liquid relative to the extraction raw material is not particularly limited, but the final extraction liquid is about 50 to 250 parts by weight, more preferably 100 to 200 parts by weight, and still more preferably about 200 parts by weight. The amount is preferably 1000 parts by weight. The extract obtained by such an operation becomes a liquid having a concentration suitable for oral administration as it is. It is preferable to use the same container as that used for extraction with water alone. The extraction operation is preferably performed using a reflux condenser.
[0022]
In addition, when extracting using alcohol, the component which is not extracted only with water will be extracted. The basidiomycetous ethanol-containing extract contains triterpenoids, nucleosides (adenosine, guanosine), and polysaccharides include hetero-β-glucan, xylo-β-glucan, and manno-β-glucan.
[0023]
The following operation can be mentioned as a typical example in the case of extracting a basidiomycetous raw material with a water / alcohol mixture.
To about 200 g of the basidiomycetous material (preferably fruit body, preferably in the form of strips) crushed into pieces, add 35 v / v% ethanol solution to 1000 ml, and maintain this solution at about 70 ° C. Cook for about 30 hours. After 30 hours, hot water (or water) is added so that the total amount becomes 1000 ml. It is preferable that the alcohol concentration finally becomes about 20 v / v%.
[0024]
The obtained various extracts are filtered or the like as necessary. The extract may be used as an active ingredient as it is. Moreover, it can also concentrate and concentrate as needed, and this can also be made into an active ingredient. Further, by evaporating the water, an extraction component as a solid content can be obtained, and further, a desired dry extraction component can be obtained by drying or the like as necessary. In addition, when using 2 or more types of extraction components as a basidiomycete, it can also extract separately, respectively, and can also extract 2 or more types simultaneously.
Furthermore, in consideration of the dosage form and dosage form of the present composition, an additive for stabilizing the formulation or the extraction component can be added when the extract is concentrated or dried.
[0025]
(Active ingredient derived from roots of Argiaceae)
The composition of the present invention can contain only the roots of a specific species of Araceae as an active ingredient. Alternatively, in addition to using the specific species as an active ingredient, the roots of other Araceae plants can be contained as active ingredients.
The specific species of the Araceae plant in the present invention is bamboo ginseng (Ginseng, P. japonicus CA Meyer or Japan Panax ginseng). This plant root alone has high antitumor activity. Accordingly, an anti-neoplastic composition containing only this type of root is also provided.
Other medicinal carrots of the family Araceae include Panax ginseng CA Meyer, American ginseng (P. quinquefolium L.), Radix Notoginseng (P. notogingseng) and others. In the present invention, one kind or a combination of two or more kinds of these can be used, but plant roots selected from ginseng and related species thereof (hereinafter referred to as roots of ginseng) and chikusetu. It is preferable to use plant roots selected from carrots and related species (hereinafter referred to as roots of chixetsu carrots, etc.). These carrot roots are usually available in a dry state, including ginseng and chixet carrots and the roots of these closely related plants. Alternatively, it can also be obtained as an alcohol immersion liquid, paste, or extract.
[0026]
The roots of the Argiaceae plant may be so-called plant roots, or may be cultured cells. In the case of cultured cells, root-derived cultured cells are preferred. In addition, if the raw material derived from the root of a plant body is included, the raw material containing other parts other than the root of a plant body can also be used.
[0027]
The composition of the present invention only needs to contain the root of the family Araceae as an active ingredient. Therefore, in the case of plant raw materials, the roots of Araceae plants may be contained as they are as active ingredients in solid form such as powder or strips.
Further, when derived from cultured cells, it may be contained as a liquid such as a culture solution containing cultured cells, or as a solid such as a dried cell product.
[0028]
(Preparation method of root extract components of Araceae plants)
Moreover, the composition of this invention can contain the active ingredient obtained by extracting only the specific seed | species of the root of Araceae plant. Alternatively, it is possible to contain, as an active ingredient, an extract component obtained by extracting the fruiting bodies of the above-mentioned basidiomycetes and the roots of the family Araceae using water and / or alcohol.
In the extraction, it is preferable to pulverize the carrot roots of the plant body to obtain powder or strips. More preferably, it is a piece of about 7 mm square or less, more preferably about 4 mm to about 6 mm, and most preferably about 5 mm. A strip shape of 0.5 mm to 2 mm square may be preferable. When using cultured cells as a raw material, it is preferable to use a dry powder.
[0029]
When extracting with water, the water to be used is not particularly limited, but it is preferable to heat the extraction. Preferably, it is about 80-about 100 degreeC, More preferably, it is about 90-about 95 degreeC.
Further, the amount of water relative to the extraction raw material is not particularly limited, but is about 5 to about 25 parts by weight, preferably about 10 to about 20 parts by weight, more preferably about 20 parts by weight after extraction. It is preferable to set the amount of water for extraction so that the amount of water is about 400 to about 600 parts by weight. This is because when the final extract is prepared in this concentration range, it is a concentration preferable for oral administration as it is, and effective extraction is performed. More preferably, it is about 500 parts by weight.
[0030]
When the heat extraction operation is performed in a state that allows a reduction due to the evaporation of moisture, the initial amount of extraction water is set assuming a reduction in moisture during that time. For example, the amount of water is extracted by increasing it by about 50 to 60% with respect to the final amount of water. On the other hand, when the heat extraction operation is performed in a state where a reduction due to evaporation of moisture is not allowed, the extraction water amount to be finally obtained may be added to the raw material from the beginning and heated. Preferably, a reflux condenser is used.
[0031]
For the heating extraction operation, an open-air container or a reflux condenser can be used. In particular, in order to inhibit the extraction of the active ingredient and maintain the effectiveness of the extracted ingredient, it is preferable to use a material obtained by painting or processing a metal part with a corrosion-resistant coating, in addition to glass, enamel, or ceramic.
[0032]
The following operation can be mentioned as a typical example in the case of extracting the roots of the Argiaceae plant which is a plant body with water.
800 ml of hot water is added to about 20 g of dried carrot roots crushed into small pieces, and decocted so that the remaining amount of the extract becomes 500 ml within the heating range described above. Preferably, the temperature of the extract is maintained at 90 to 95 ° C., and the heating is adjusted to 500 ml in about 3 hours. In particular, it is preferable to boil and extract the same amount of carrot raw material with 500 ml of water using a reflux condenser for about 2 hours. The extract obtained by such an operation becomes a liquid having a concentration suitable for oral administration as it is.
[0033]
In the case of extracting with alcohol, it is preferable that the alcohol to be used is a drinkable alcohol in consideration of the case where it may be drunk as it is. That is, it is preferable to use ethanol.
When extracting using alcohol, you may extract only with alcohol, However, It is preferable to mix with water and to extract with a liquid mixture. The alcohol concentration is preferably 50 v / v%, more preferably about 35 v / v%.
The alcohol-containing extract is not particularly limited, but is preferably heated during extraction. Preferably, it is 50-80 degreeC, More preferably, it is 75 degreeC or less, More preferably, it is 70 degreeC or less.
Although the extraction time is not particularly limited, it is preferably 10 hours or longer, more preferably 20 hours or longer, and most preferably about 30 hours or longer.
It should be noted that the alcohol concentration is preferably about 20 v / v% at the final oral administration. For example, in the case of extraction with an alcohol solution having a concentration exceeding 20 v / v%, it is preferable to dilute with water after extraction so as to be 20 v / v%.
Further, the amount of the extraction liquid with respect to the extraction raw material (carrot root) is not particularly limited, but it is preferable that the extraction liquid is 1000 ml with respect to 200 g of the raw material. It is preferable to use the same container as that used for extraction with water alone. The extraction operation is preferably performed using a reflux condenser.
[0034]
The following operation can be mentioned as a typical example in the case of extracting the roots of Araceae plants with a water / alcohol mixture.
To about 200 g of dried roots of Araceae plant crushed into small pieces, a 35 v / v% ethanol solution is added to make 1000 ml, and this solution is maintained at about 70 ° C. and decocted for about 30 hours. After 30 hours, hot water (or water) is added so that the total amount becomes 1000 ml. It is preferable that the alcohol concentration finally becomes about 20 v / v%.
The extract obtained by such an operation becomes a liquid having a concentration suitable for oral administration as it is.
[0035]
The obtained extract is filtered or the like as necessary. The extract may be used as an active ingredient as it is. Moreover, if necessary, it can be concentrated to give a concentrate, which can be used as an active ingredient. Further, by evaporating the water, an extraction component as a solid content can be obtained, and further, a desired dry extraction component can be obtained by drying or the like as necessary.
In addition, the root of Araceae plant can use not only one type but two or more types. In that case, an extract may be prepared for each type, or an extract in any combination of two or more types. May be prepared.
Furthermore, in consideration of the dosage form and dosage form of the present composition, an additive for stabilizing the formulation or the extraction component can be added when the extract is concentrated or dried.
[0036]
In addition, when using 2 or more types of basidiomycetes as a basidiomycete raw material, an extract can be prepared separately for each type, but can also be extracted simultaneously. The same is true for Argiaceae plants. Furthermore, the basidiomycete raw material and the root raw material of the family Argiaceae can be extracted independently, but the raw material composition containing both can be extracted with the same extraction medium and extracted simultaneously. Preferably, simultaneous extraction is performed. Even in the case of simultaneous extraction, the extraction conditions for the above-mentioned basidiomycete raw materials or root raw materials of Argiaceae plants can be employed. In addition, the active ingredient by extraction of the basidiomycete raw material and the root raw material of the family Argiaceae with water and the active ingredient by extraction with a water / alcohol (ethanol) mixture may be mixed to form the present composition. In this case, it does not matter whether simultaneous extraction is performed, but simultaneous extraction is preferable for each of water extraction and water / alcohol extraction.
[0037]
A typical example of simultaneous extraction is shown below.
About 6 g of the fruit body of a specific species of Amanita bamboo crushed into a strip of 7 mm square or less, preferably about 5 mm, about 6 g of a fruit body of a specific species of agaric bamboo crushed into a strip shape, Preferably, 800 ml of hot water is added to about 6 g of root of chixet carrot, and decocted so that the remaining amount of the extract becomes 500 ml within the heating range described above. Preferably, heating adjustment is performed so that the amount becomes 500 ml in about 3 hours. The extract obtained by such an operation is a liquid having a concentration suitable for oral administration as it is.
In addition, a 35 v / v% ethanol solution was added to a raw material mixture having the same form and composition (however, 60 g fruit body of Mannentake, 60 g fruit body of Kawaratake, 60 g of Argiaceae) to make 1000 ml of this solution. Is decocted for about 30 hours. After 30 hours, hot water (or water) is added so that the total amount becomes 1000 ml. It is preferable that the alcohol concentration finally becomes about 20 v / v%.
The extract obtained by such an operation is a liquid having a concentration suitable for oral administration as it is.
Since these two kinds of extracts each contain the raw material-derived component of the present invention, they can be provided alone as the present composition, but preferably only the water extract or the water extract Is used relatively much.
On the other hand, two kinds of extracts can be mixed in advance to make this composition as one preparation, or two kinds of preparations to be mixed at the time of administration, or they can be administered separately but simultaneously. Provided as a set of planned formulations.
[0038]
In addition, the composition of the present invention comprises an extractive component of basidiomycetes with water and / or alcohol and an extractive component of roots of sugiaceae plants with water and / or alcohol. It can combine suitably so that an origin active ingredient may be included. For example, a water extract component of basidiomycete and an alcohol extract component of the root of the family Araceae can be combined.
As described above, in the final administration stage, if the basidiomycete-derived active ingredient and the root-derived effective ingredient are contained, the composition is composed only of one type of preparation. Alternatively, the present composition can be constituted by a combination of two or more kinds of preparations.
[0039]
In the present invention, it is sufficient to have an effective ingredient derived from basidiomycetes, or in addition to this, it is sufficient to contain both an effective ingredient derived from the roots of the family Araceae, and as long as these effective ingredients are contained, the effective ingredients are Even in the form of an extract, it may be in the form of raw materials such as fruiting bodies and roots, or a pulverized product thereof.
In addition, in the case of a composition containing an active ingredient by containing the raw material itself instead of the extract, it can be administered for food and the like itself, but it can be used for obtaining an antitumor extract. It can also be provided as a composition for extraction.
In the case of a composition containing an active ingredient in the form of an extract, it is expected that the composition can be more effectively absorbed and the substantial dose (in terms of raw material) can be reduced. Accordingly, the composition of the present invention is preferably a composition containing an active ingredient as an extract fraction.
In addition, an active ingredient is contained as an extraction fraction and / or raw material itself, Comprising: It does not contain only in any one form, It selects as needed.
[0040]
The composition of the present invention is included in the composition of the present invention as long as it is composed at the following weight ratio of raw material compositions (the ratio in terms of dry weight).
A preferred first ratio of the active ingredients corresponds to the weight (dry weight) ratio of the extraction raw material.
The raw material composition ratio of the basidiomycete and the root of the family Argiaceae is preferably 4 to 6: 8 to 12: 2.4 to 3.6 in terms of the weight ratio of the roots of Mannentake: Kawaratake: Araceae. When the weight ratio is within this range, preferable antitumor activity is exhibited. More preferably, it is 4.5 to 5.5: 9 to 11: 2.7 to 3.3, and more preferably about 5: about 10: about 3.
[0041]
Also, the preferred second ratio is the weight ratio (dried) of the roots of Mannentake: Kawaratake: Argiaceae 4.8-7.2: 4.8-7.2: 4.8-7.2. Preferably there is. When the weight ratio is within this range, good antitumor activity is exhibited. More preferably, it is 5.4 to 6.6: 5.4 to 6.6: 5.4 to 6.6, and more preferably about 6: about 6: about 6.
[0042]
A third preferred ratio in the composition of the present invention is that the raw material composition of the roots of the garlic mushrooms: Kawaratake mushrooms is about 28 to about 33: about 56 to about 33: about 17 to about 33 by weight. A range is preferable. In this range, the antitumor activity is higher than the composition of the raw material composition in a weight ratio of about 56: about 28: about 17.
[0043]
In addition, the composition of the present invention may contain basidiomycetes or, in addition to this, the roots of the family Argiaceae, but does not preclude containing other active ingredients. Other active ingredients can be included as long as they do not interfere with the synergistic action of the two components in the composition of the present invention.
[0044]
In any of the above-described formulations, preferably ginseng or chixetsuninjin is used as the araceae plant. In particular, chixet carrot is preferably used. In these examples, a preferable composition can be obtained even if chixet carrot is changed to ginseng.
[0045]
The composition of the present invention (which may be a single component-containing composition or two or more component-containing compositions) has antitumor activity, particularly leukemia, lymphoma, cervical cancer, lung cancer, ovarian cancer. Antitumor activity against breast cancer (metastasis), skin cancer (metastasis), breast cancer, skin cancer. Examples of leukemia include erythroblastic leukosis. Therefore, the composition of the present invention can be used as a composition for tumor treatment (tumor treatment agent) in mammals such as cattle, horses, dogs, cats and the like including humans. In particular, the composition is preferably used as a tumor treatment composition for leukemia or lymphoma.
The composition of the present invention is an immunostimulatory composition or a composition effective for obesity, myocardial infarction, arteriosclerosis, hyperlipidemia, hypercholesterolemia, and hypertension.
Since the composition of the present invention contains a specific basidiomycete as an active ingredient, good antitumor activity has been found. Therefore, the present invention which contains the basidiomycetes identified in the present specification as active ingredients even in compositions containing the basidiomycetes and roots of the family Arcoceae as active ingredients that have already been found by the present inventors. This composition provides a highly stable antitumor activity as compared with the antitumor activity of each individual.
[0046]
Moreover, since the composition of this invention has antitumor activity, it can also be used preventively by administering to the patient who is at risk of tumor onset, or the patient at risk of recurrence. Therefore, according to the present invention, there is also provided a preventive pharmaceutical composition (tumor preventive agent) for the purpose of preventing recurrence or onset of tumor.
[0047]
In addition, the composition of the present invention has no side effects and is characterized by reducing and eliminating the side effects of other drugs.
In particular, when used as an antitumor agent, there are various effects such as reduction of pain, improvement of appetite, and good sleep in addition to tumor healing or degeneration. In addition, the composition of the present invention has a hypoglycemic action, and when used as a hypoglycemic agent, in addition to lowering the blood glucose level, alleviation of body pain, in particular, headache and numbness of the limbs are greatly reduced. Effects, such as improvement, appetite enhancement, vision recovery, stress reduction, comfortable sleep.
[0048]
As an active ingredient in the present invention, a basidiomycete or an extract thereof and a root of the family Araceae or an extract thereof are mixed with a pharmaceutically acceptable carrier or additive as a pharmaceutical composition in a form suitable for administration. used. The form of the composition is not particularly limited, but liquids, syrups, suspensions, powders, granules, tablets, pills, capsules, emulsions, troches, chewables, suppositories, eye drops, injections, aerosols It can be formulated into elixirs and the like. Moreover, it is also preferable to use a solid (powder) agent that can recover the liquid material by adding water during use.
[0049]
Moreover, the composition of this invention can be administered orally or parenterally. Preferably, it is administered orally. The general dosage for oral administration is shown below. The dose is appropriately set according to symptoms, individual physical strength, and the like.
That is, as a general dose (ordinary dose), for example, basidiomycetes (total weight of raw materials of Mannentake and Kawaratake) 200 mg to 2 g or root of Argiaceae plant 200 mg to 2 g per kg body weight, or In addition to the basidiomycete content, extract components from 100 mg to 1 g of the root family of Araceae plants are taken as one day, and each is administered once to 3 times a day.
In particular, when used as an antitumor agent containing either the basidiomycete or the root of the family Argiaceae, it is preferable to contain an extract component from 0.1 to 1 g of the root of the basidiomycete or the family Argiaceae. In addition to the basidiomycete roots in addition to basidiomycetes, in addition to the basidiomycete content, extract components / kg body weight / day from 0.05 to 0.5 g of the root family of urchinaceae It is preferable that More preferably, an extract component of 0.3 to 0.5 g of roots of basidiomycetes or araliaceae plants / kg body weight / day, or 0.15 to 0.25 g of roots of araceae plants in addition to the content of basidiomycetes Extracted component / kg body weight / day.
[0050]
Since the composition of the present invention has extremely low toxicity and does not cause serious side effects, it can be safely administered even at high doses depending on the symptoms.
A preferred dosage form for oral administration is a solution or syrup. The medium is preferably water.
[0051]
While the composition of the present invention has the above-described antitumor activity, it has little or no side effects. Furthermore, it also has a health promotion effect. Therefore, the present composition is not only a therapeutic or preventive pharmaceutical composition for the purpose of antitumor or tumor prevention, but also an oral or parenteral health promoting composition, nutritional supplement composition or It can be used as a food composition. That is, by taking the composition of the present invention by eating and drinking or tube feeding, it is possible to provide a healthy life and improvement of the quality of life.
From the above, the composition of the present invention can also improve QOL during diseases such as tumors, hypertension and diabetes. Therefore, the composition of the present invention can be used as an oral or parenteral health promoting composition, nutritional supplement composition, or food composition composition for improving QOL in various diseases.
The composition of the present invention has a growth-inhibiting action or killing action against bacteria that cause food poisoning, such as Escherichia coli, Salmonella, Clostridium botulinum, Helicobacter pylori, and O-157.
The amount of intake is not particularly limited, but is preferably lower than the above-mentioned dose for treatment or prevention. It can be set to about one-several to one-fifth.
[0052]
When the composition of the present invention is used for health promotion, nutritional supplement or food, it may be liquid or solid. Oral tablets, capsules, chewables and the like can be formulated as appropriate. Moreover, it is also preferable to use a solid (powder) agent that can recover the liquid material by adding water during use. Furthermore, it can also be set as a form which can be ingested tube feeding or via an intestinal tract. Selection of such forms and formulation according to the selection are well-known matters for those skilled in the art.
[0053]
【The invention's effect】
According to the present invention, a composition having excellent physiological activity, in particular, antitumor activity is provided. This composition is not only effective for the treatment of tumors (cancer), but can also be used prophylactically by administration to patients at risk of developing tumors because it has very low or no side effects. Moreover, it is effective not only for treatment or prevention but also as a health promotion composition or a nutritional supplement composition.
[0054]
【Example】
Example 1
Preparation of various composition stock solutions and dilutions
Hereinafter, the present invention will be described with specific examples. However, the present invention is not construed as being limited to these examples.
(Test 1: Antitumor activity)
(Preparation of various composition stock solutions)
Examples The fruit bodies of Ganoderma pheifferi and Ganoderuma lipsense, Kawaratake (Trametes hirsuus), and Chikutsuninjin (Japan Panaxginseng) were used as raw materials for the examples. Mannentake used was a natural, mature black fruit body that was air-dried at room temperature avoiding light. Kawaratake was a natural fruiting body that was air dried at room temperature avoiding light. Chixetsu carrots were taken from Japanese adults sleeping in summer and air dried at room temperature avoiding light. In addition, as a control raw material, a fruit body of Ganoderma lusidum and a fruit body of Kawaratake (Trametes versicolor, Coriolous Versicolor) were used. Mannentake used was a natural, mature black fruit body that was air-dried at room temperature avoiding light. Kawaratake was a natural fruit body collected in Japan in the summer and air-dried at room temperature avoiding light.
[0055]
In either case, 500 ml of water was added to 20 g of each raw material crushed into small pieces of about 5 mm square, and boiled for 2 hours while refluxing using a reflux condenser. Then, it filtered and obtained 4 types of extraction stock solutions.
In addition, per 1 ml of each extraction stock solution contains an extraction component corresponding to 0.04 g of each raw material weight.
[0056]
Further, each of these extraction stock solutions was prepared in dilutions of 1/4, 1/8, 1/16, 1/32, 1/64, 1/128, and 1/256 with water.
[0057]
(Test Example 2)
Confirmation of antitumor activity
Confirmation of Cell Proliferation Inhibitory Effect on K562 Cell Line which is Human Leukocyte Cancer Cell 150 μl of K562 cell line culture solution (20 × 10 cells) ^Three ) Was added to the recesses of the microtiter plate. 50 μl of various dilutions obtained from the extraction stock solutions of 4 types of Example samples and 2 types of control samples are added to the recesses to which K562 cells have been added, and at 37 ° C., 24 hours, 48 hours, 72 hours, and 96 hours. The cells were cultured, and after each culture period, the cell growth state was measured by the SRB method.
The SRB method was performed as follows. 50 μl of 80% trichloroacetic acid was added to each recess of the microtiter plate to immobilize the cells for 1 hour. Thereafter, it was washed 4 times and sufficiently dried. 200 μl of 4% SRB (sulfodamine B) was added to each recess and the cells were stained for 30 minutes. Thereafter, it was washed 4 times and dried. 10 mM unbuffered Tris base was added to each recess and stirred for 5 minutes. Then, the light absorbency in wavelength 490nm was measured about each liquid in a recessed part. The ratio of the initial cell number to the cell number after the lapse of the predetermined culture time obtained from the measurement result was defined as the growth inhibition rate (%).
Tables 1 to 4 show the results for each of the dilutions obtained from the four types of sample samples. Tables 5 and 6 show the results for each of the dilutions obtained from the two types of control samples. Shown in
[0058]
Cell growth inhibition rate of Ganoderma pheifferi
[Table 1]

Cell growth inhibition rate of Ganoderma lipsiense
[Table 2]

Cell growth inhibition rate of Tramates hirsutus
[Table 3]

Panax japanicus CAMeyer) cell growth inhibition rate
[Table 4]

Cell growth inhibition rate of Ganoderma lucidum
[Table 5]

Cell growth inhibition rate of tramates versiclolor
[Table 6]

[0059]
As can be seen from these results, all of the sample samples (Tables 1 and 2) exhibited cell growth inhibition rates that were approximately the same as or higher than those of the control sample (Table 5). In particular, it was stable and showed a high cell growth rate at the culture time of 72 hours and 96 hours.
In addition, Kawaratake (Table 3) of the example sample showed almost the same cell growth inhibition rate as Kawaratake (Table 6) of the control sample.
From these facts, it was clear that the hot water extract of Mannentake and Kawaratake of the sample samples had antitumor activity alone.
In addition, Chixetsu carrot also had a cell growth inhibitory rate equal to or higher than that of Mannentake, Kawaratake and control basidiomycetes in Examples. That is, it was clear that chixetsu carrot alone has antitumor activity.
Therefore, it has been found that a composition containing these specific types of garlic mushroom and / or kawatake mushroom and / or chikutsuninjin as active ingredients can be used as a therapeutic or prophylactic agent having antitumor activity. In addition to these basidiomycetes, a composition containing a composition containing aspergillus roots (typically medicinal carrots such as chixet carrots) as an active ingredient can be used for treatment with antitumor activity or It was found that it can be used as a preventive agent.

Claims (5)

An antitumor composition having antitumor activity, comprising Ganoderma p f eifferi as an active ingredient. In addition, Ganoderma lipsense and / or Traets containing hirsutus as an active ingredient,The antitumor composition according to claim 1. Furthermore, the anti-tumor composition of Claim 1 or 2 which contains the root of the plant which belongs to the urchinaceae family as an active ingredient . 4. The antitumor composition according to claim 3, wherein the roots of the araliaceae are one or more selected from the group consisting of ginseng, chixetine carrot, and densit carrot. A method for producing an antitumor composition, wherein a raw material containing Ganoderuma p f eifferi is extracted with water or a mixture of water and alcohol.