JPS6185324A - Production of carcino static agent - Google Patents

Abstract

PURPOSE:To obtain a carcinostatic agent from a water extracted solution of a dried substance of it, by using Sophorae subprostratae radix and Crocosmia aurea Plach. as bases, and blending them with fruit of Opuntia Ficus-indica Mill. var. Cacti to give the water extracted solution. CONSTITUTION:Sophorae subprostratae radix(plant belonging to the genus Sophora of Leguminosae) and Crocosmia aurea Plach(perennial root native to South Africa having about 1m height) are used as bases, and blended with Opuntia Ficus-indica Mill. var. Cacti(perennial rot native to Mexico, having stalk reaching 2m, elliptic, flat, and thick stem node) fruit, or Pseudocydonia sinensis Schneid(deciduous tall tree native to China) fruit, Trapa natans L. var. bispinosa(annual root growing in pond or swamp) fruit, and Panax Schinseng Nees(perennial root native to China and Korea) to give a water extracted solution of the blend or a dried substance of it. A carcinostatic agent containing the water aqueous solution or the dried substance of it as an active ingredient. Combination of each component provides synergistic effect.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention provides: (1) A method for producing an anticancer agent consisting of an aqueous extract or a dried product of a product obtained by adding cactus fruits to wild bean root and narcissus as base materials (2) ) This invention is an invention of a method for producing an anticancer agent comprising an aqueous extract of the above base material to which cauliflower seeds, white nuts, and bitter ginseng (medicinal ginseng) are added or a dried product thereof.

The wild bean root used in the present invention is a plant belonging to the Fabaceae family, genus Ophora, and is currently mainly imported from China, and its seedlings have a bean-like shape.

Crocosmia aurea
Plach is a perennial herb that grows around 1 meter in height and is native to South Africa.

Cactus (0puntia Ficus-indica)
Milivar Saboten) is a perennial herb native to Mexico, and its stems reach a height of 2 meters, and are characterized by having oval, oblate, and thickened stem nodes.

Pseudocydonia 5inensi (Pseudocydonia 5inensi)
Schneid) is a deciduous tree native to China that reaches a height of 8 meters, and its fruit is a large f! ``■The fruit is l-shaped and has hard flesh.

Trapa natans 1. ,var,B1
5pinosa) is a book that grows in ponds and swamps, and its fruit has thorns.

Panax Schinsen (Medicinal ginseng)
Neesl is a perennial herb and medicinal plant with a height of about 60 cm that is native to China, Korea, and Korea.

Example 1 10g of 111 bean root (whole plant placed in 121 places) 40g of Daffodil 30B-1 Naboten was extracted with water (
) to obtain 500 ml of extraction 11.

100 ml of this was freeze-dried to obtain 0.8550 g of dried extract.

Example 2 Yama bean root (1 dried whole plant) 10g Daffodil 25g Karin 10g Elbow fruit 10g Carrot
Extract 8g with water,
600 ml of extract was obtained.

Of the total, 75III was freeze-dried to obtain 1.0870 g of dried extract.

The anticancer effect of the invention product according to the method of the present application was tested as follows, and the effect was confirmed.

The extract obtained by J in claim 1 below is VR.
-3, the extract obtained according to the second paragraph is abbreviated as V R-H.

Experimental animals include ICR-3l) [5-week-old mice (purchased from Hamamatsu Experimental Animal Cooperative), IT! I used ¥5 each.

After incubation in each macus, 0.2ml (5x 105 cells) of a cell suspension prepared from 7 days old Sarcoma 180 ascites at 2.5 After 1 hour, pour 01 ml or 02 ml of the drug solution into the 11M cavity once, insert a step between 711 and 11, and observe whether the patient is alive or dead.
child.

The feed used was solid feed (manufactured by A Riental Yeast Co., Ltd.)17, and water was provided ad libitum.

All of the comparative experiment control 11Y (Mudan'J I!Y) died within 19 days, and the average number of survivors was 128±44 days.

VR-11 freeze-dried extract 2 mg/0, 2 m
The average survival time for the administration group was 3 days.
The survival time was 94±231 days, and an extremely excellent life-prolonging effect was obtained with a T/C% of 307.8. Moreover, the results of the examination of the two mice that survived for 60 days showed no accumulation of ascites or the presence of solid tumors, and internal organs i! #No macroscopic changes were observed in the organs.

VR-3 freeze-dried extract l, 5 u mouth 10.2++
+1 saline/mouse/day, average survival days 2 in the 7-day treatment group
Although a statistically significant survival effect of 8.6±9 [1 and T/C% of 223.4 days was observed, no mice survived for 60 days.

As mentioned above, animal experiments have confirmed that the product of the present invention has shown remarkable results as an anticancer agent.

In addition, the methanol-soluble part of wild bean root (alkaloid fraction)
In the 1 mg 10.2 ml saline mouse/l'l, 7-day administration group, the average number of surviving mice was 14.2 26 FI, T/'C% 11
0.9, and no significant survival effect was observed for gunshot wounds.

Mountain bean root methanol soluble part (both alkaloid parts) 0.
In the 5 mg/0.1 ml saline/mouse/1.7-day administration group, the average survival time was 29.4 ± 22.5 days, and T
/C%229,8.60 Although the wound findings of one mouse surviving during F1 were normal, no statistically significant survival effect was observed.

Mountain bean root methanol insoluble part (non-alkaloid fraction) 2
mg/0, 2ml saline/mouse/day, 7 days administration group, mean survival days 31.0±17.1E+, 17C%
242.2, which showed a very significant survival effect, but one mouse that survived for 60 days had ascites mixed with a significant amount of blood and fava beans in the aperture. A large solid tumor was detected.

Bittersweet XAr)-2 adsorbed ethanol elution fraction (alka[I
1mg/0.2ml saline/mouse/1
．． 7] -1 stage group, mean survival days 23.2 ± 20
．． On day 6, T/C% was as high as 18] and 3, but no significant difference was observed in the gunshot wound, and 1 mouse survived for 60[1].
14.0±1.6 days T/C except for (normal finding results)
%109゜4, which is completely invalid.

Bitter Ginseng
In the group administered with Il saline/mouse/day for 7 E1, the effect was completely ineffective with an average survival time of 14.0±1.411.176%107.4.

In the 7-day treatment group, the average survival time was 46.2 ± 18.5 days, T
/C%, which was an extremely high value of 360.9, and was recognized as having a significant survival effect according to syntax 1, but giant solid tumors were observed in two mice that survived for 60 days.

Panax ginseng
A statistically significant survival effect of 1.4 days and 170% 298.4 days was observed, and no abnormality was found in the wounds of mice that survived for 60 days.

Might Mine So C (MMC) 0,02 mg/
When mice were administered for 7 days per day, the average survival time was 50.
3-1=11.8[3,'r/C% showed the highest value of 392.2, indicating an extremely excellent life prolonging effect. However, one of the two mice that survived for 60 days had a large amount of ascites containing blood and a large solid tumor. The other one was normal.

The results of the following two comparative experiments show that both VR-H and VR-3 complex herbal medicine aqueous extracts obtained by the production method of the present invention have an excellent survival effect on ascites cancer in mice, which is due to the present invention. It had a remarkable effect compared to extracts of wild bean root and bitter ginseng, which are the constituent components of ginseng.

Claims (2)

[Claims] (1) The base material is wild bean root (Sophora genus) and daffodil (Crocosmia aurea Plach.), and cactus (Opuntia ficu) is used as the base material.
s-indica Mill. var. Saboten
) fruit added to the aqueous extract or its dried product. (2) The base materials are wild bean root (Sophora genus) and Crocosmia aurea Plach.
sinensis Schneid) fruits, caltrops (T.
rapa natans L. var. bispino
sa) fruit, bitter ginseng (Panax Schi)
A method for producing an anticancer agent comprising an aqueous extract or a dried product thereof to which nseng nees) has been added.