RU2123332C1 - Anthelminthic agent - Google Patents

Abstract

FIELD: agriculture, veterinary helminthology. SUBSTANCE: anthelminthic agent is 5-chloro-4′-- -nitrosalicylanilide. This is an amorphous powder, grayish-yellow color, water-insoluble, good soluble in acetone, benzene, alcohol and acetic acid. Proposed anthelminthic agent shows high (100%) effectiveness against cestodes in sheep, goats, dogs and other animals and can be used for animals treatment. EFFECT: high effectiveness of an agent. 5 tbl

Description

 The invention relates to the field of agriculture, namely to veterinary helminthology, and can be used to treat animal cestodoses.

 A known tool for the treatment of cestodoses of animal copper sulfate, which is used in the form of a 1% solution in doses of 15-100 ml per sheep and 2 ml / kg of body weight of cattle [1].

 The disadvantages of this drug are low efficiency and relatively high toxicity.

 Other means for treating animal cestodoses are also known - the dosage forms of phenasal: phenalidone and fensulfan, which are respectively a mixture of phenasal with polyvinylpyrrolidone in a ratio of 4: 1 and a mixture of phenasal with sodium dodecylbenzene sulfonate - 3: 1, which are used in doses of 60-75 mg / kg [2, 3].

 The disadvantages of these drugs include the use of deficient carriers as excipients.

 The closest technical solution to the claimed is the drug phenasal (I), which with cestodoses of animals is used in doses of 0.1-0.25 g / kg [1].

 The disadvantages of the drug include the complex and expensive synthesis of phenasal from inaccessible raw materials (2-chloro-4-nitroaniline), a high therapeutic dose and deficiency.

 The proposed anthelmintic agent is 5-chloro-4'-nitrosalicylanilide (II), which is used to control mollusks (4).

 This compound is much simpler and cheaper to synthesize than phenasal.

Применение известного препарата-моллюскоцида-5-хлор-4' нитросалициланилида в качестве антгельминтного средства соответствует критериям "новизна" и "существенные отличия".

The use of the known preparation molluscocide-5-chloro-4 'nitrosalicylanilide as an anthelmintic agent meets the criteria of "novelty" and "significant differences".

 The proposed anthelmintic agent 5-chloro-4'-nitrosalicylanilide (cesal) is prescribed to animals with cestodoses by oral administration in doses of 0.05 - 0.1 g / kg body weight.

 Example 1. The study of toxicity cesala.

 The drug was tested for tolerance in 4 lambs at doses of 0.5 and 0.75 g / kg, 2 kids - 0.5 g / kg and 2 outbred dogs at a dose of 0.5 g / kg body weight. The animals were observed for three days. Signs of toxicosis were not noted.

 Example 2. The test cesala with experimental hymenolepidosis of white mice.

 The experiment was carried out on mice. Cesal was orally administered to 5 animals in suspension in a 1% starch paste at a dose of 0.1 g / kg. Phenasal was tested in the same dose as a basic preparation. There were also 5 mice in the control group.

 The results of the experiment are shown in table 1. The drug cesal and phenasal in the tested doses showed 100% effectiveness.

 Example 3. The test cesala with moniesiosis of sheep.

 An experience. 1 The drug was tested on 9 lambs naturally invaded by moniesiae (species M.expansa). 4 lambs (experimental group) were injected orally at a dose of 0.05 g / kg body weight in the form of a 1% starch suspension, and 5 animals were left as a control.

 5 days after the administration of the drug, animals of the experimental and control groups were slaughtered and helminthological dissection of the intestines was performed. It was found that in the test dose, Cesal completely cured all the lambs from moniesiosis. In animals of the control group, an average of 2.8 individuals were found per head. cestode.

 The results of the experiment are shown in table. 2.

 Experiment 2. The test of Cesala was carried out on 12 lambs spontaneously infected with moniesia, which were divided into 4 groups of 3 lamb each equivalent in weight and infection. Lambs of groups I, III, and IV were invaded by M.expansa, II - M.benedeni.

 Lambs of the first experimental group were injected with cesar orally in a 1% starch paste at a dose of 0.05 g / kg body weight, the second and third at a dose of 0.75 g / kg.

 The animals of the fourth group (treated control) were administered the basic drug phenasal at a dose of 0.1 g / kg.

 The effectiveness of deworming was determined by the method of "critical test", determining the number of eggs of cestodes in 1 g of feces before treatment and 10 days after the use of drugs.

 The drug cesala at a dose of 0.05 g / kg from three animals completely cured two lambs and one partially: the number of secreted eggs decreased by 86.7%; at a dose of 0.075 g / kg, he completely cured 6 lambs of the second and third experimental groups, infested with cestodes M.benedini and M.expansa, respectively (EE = 100%).

 From the use of the basic drug phenasal in the recommended dose for individual use of 0.1 g / kg body weight, 100% efficiency against M.expansa was also obtained.

 The results of the experiment are presented in table.3.

 Example 4. The test cesala with moniesiosis of goats.

 Cesal was tested at a dose of 0.075 g / kg on three goats spontaneously infected with M.expansa. The drug was administered to animals as in the previous experiment. Efficiency was evaluated by the "critical test" method.

 Cesal at a dose of 0.075 g / kg completely cured all the kids (EE = 100%).

 The results of the experiment are given in table.4.

 Example 5. The test cesala with cestodosis of dogs.

 Cesal was tested on puppies of 3 months of age weighing 3-5 kg spontaneously invaded by cucumber tapeworm (Dipilidium caninum). Invasive animals were selected in the experiment on the presence of segments of cestodes in feces. A total of 6 puppies were used, which were divided into equivalent three groups of 2 animals each.

 Puppies of the first experimental group were asked to be guided at a dose of 0.05 g / kg body weight twice after 8 hours by individual free feeding with minced meat, the second group - at a dose of 0.01 g / kg once with minced meat.

 The animals of the third group (treated control) were given the basic drug phenasal at a dose of 0.1 g / kg once with minced meat.

The effectiveness of deworming was determined by the method of "critical test", determining the presence of segments of cestodes in the feces before treatment and 10 days after the use of drugs,
As a result, it was found that he combed at doses of 0.05 g / kg twice and at a dose of 0.1 g / kg of body weight once completely cured puppies of dipyldiosis - the efficiency is 100%.

 Similar efficacy was obtained from the use of the basic drug phenasal at a dose of 0.1 g / kg.

 The results of the experiment are presented in table.5.

 Sources of information.

 1. Instruction "Measures for the prevention and elimination of animal diseases with helminth infections." - M .: "Agropromizdat", 1989.

 2. USSR copyright certificate N 1157727, cl. A 31 K 31/00.

 3. Copyright certificate of the USSR N 1592984, cl. A 31 K 31/63.

 4. US patent N 3079297 (CL 514-166), 1963.

Claims (1)

 The use of 5-chloro-4'-nitrosalicylanilide (cesala) as an anthelmintic in animal cestodoses.

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