RU2093150C1 - Anhelminthic agent - Google Patents

Abstract

FIELD: veterinary science, helminthology. SUBSTANCE: invention relates to the use of component composition consisting of anhelminthic agent 2-acetaminobenzimidazole and dextrin taken at mass ratio = (1.0-0.6):(1.0-1.4), respectively. Agent is used for treatment animal and birds with helminthosis. EFFECT: enhanced effectiveness of agent. 4 tbl

Description

 The present invention relates to the field of agriculture, in particular, veterinary medicine and is used to treat nematodoses, cestodoses and tremodoses of animals and birds.

 There is a method of treating 2-acetaminaminobenzimidazole, referred to in the literature as acetamisole, for ruminant helminthiases (at a dose of 50 mg / kg for nematodoses, 100 mg / kg-cestodoses, 150 mg / kg twice trematode) pigs (at a dose of 150 mg / kg once, in a group method with nematodoses) of birds (at a dose of 300 mg / kg, once, by a group method with nematodoses). (Temporary instruction on the use of acetamizole for deworming of animals and birds of May 29, 1990).

 The disadvantage of this method is the teratogenic and embryotoxic effect of acetamisole in therapeutic and elevated doses, a high therapeutic dose.

 The objective of the invention is to find an effective tool that does not have a teratogenic and embryotoxic effect and at the same time increases the anthelmintic effect.

 The problem is solved by using a composition of ingredients for the treatment of animal helminthiases, which consists of the anthelmintic of acetamisole (2-acetaminaminobenzimidazole) and dextrin, named by the authors "Akzars" in the following ratio of components (wt.): Acetamizole 1.0-0.6, dextrin 1, 0: 1.4.

 The product, conditionally called "Akzars", is a light brown powder, odorless, tasteless, mixes easily with food, does not cake, does not dissolve in water, but forms a stable suspension. It is soluble in dimethyl sulfoxide, dimethylformamide, ethanol. The shelf life of the drug is up to 5 years.

 The proposed anthelmintic that does not have embryotoxic and teratogenic properties, has a wide spectrum of anthelmintic action, is effective for strong digestive tract and lung pulmonary infections, cestodoses and tremadoses in animals at doses of 30–50 mg / kg of DV in a single or double-dose oral administration individually or in group form in the form of a powder or granules mixed with food.

 The drug is highly effective in mixed helminthiases of other animal species (pigs, horses, birds, dogs, cats, etc.).

 "Akzars" was tested on sheep, pigs, chickens, dogs spontaneously infested with different classes of helminths, and showed good results.

 Below are examples of specific implementation of the method.

 Example 1

 Efficiency of an akarsa at a different ratio of components.

 Used 18 lambs weighing 30 kg, spontaneously invaded by mixed helminths, of which 6 groups were formed. The preparations were administered individually to the lambs, once in a suspension. The ratios of the components, the doses taken and the results 10 days after treatment are presented in Table 1, from which it can be seen that a high anthelmintic effect was obtained with a ratio of components of 1: 1, 2: 1, 1: 0.6 and 1: 0.8, but on the embryotropic properties in rats, positive results were obtained only with a ratio of 1: 1 and with a high dextrin content in Acarxa.

 Example 2

 The effectiveness of acarx for sheep helminthiases.

 Of the 54 infected sheep, 6 different groups were formed and different doses of acarx were tested with a single oral administration in the form of a stable aqueous suspension of 10% (Table 2).

 In case of strong digestive tract infections (haemonchiasis, nematodirosis, marshallagias, ostertagias, trichostrongiasis, haberthiosis, bunostomiasis, esophagostomiasis), a dose of 60 mg / kg (30 mg / kg in terms of DV) and higher doses of dictyocauliasis and monieziosis of 50 mg were effective / kg on DV).

 Example 3

 The effectiveness of Acarxa with dicroceliosis and fascioliasis of sheep.

 Of the 9 sheep infested with dicrocelia and fascioli, 3 groups were formed. Sheeps of the first group were administered once, individually, Acarx at a dose of 200 mg / kg (100 mg / kg in terms of DV), the second acetamisole at a dose of 150 mg / kg, and the third served as a control.

 After 30 days, all the sheep were killed and helminthological studies of the liver were performed.

Found, respectively, in the groups: diccelia-18; 37; 1120, fasciol-0; 2 and 9. At the same time, the intensification of the treatment of dicroceliosis was: Actars 98.4% acetamizole 96.7% fascioliasis, respectively, 100 and 77.8%
Example 4

 The effectiveness of acarx for horse helminthiases.

Used 15 horses infected with parascorides and strongilates, of which 5 groups were formed. The horses of the first group were fed Aktsars with food at a dose of 80 mg / kg (40 mg / kg in terms of DV), the second 90 mg / kg (45 mg / kg in terms of DV), third
100 mg / kg (50 mg / kg DV). The horses of the fourth group received acetamizole at a dose of 50 mg / kg, and the fifth served as a control. As a result (Table 3), a high anthelmintic effect was obtained with parascaridosis and strongilatoses from all doses of the claimed complex. The effectiveness of the base drug was lower.

 Example 5

 The effectiveness of Acarxa in pig nematodes.

 On 30 infected pigs, the anthelmintic efficacy of Acarx was studied for DV from 75 to 100 mg / kg in comparison with acetamizole at a dose of 100 and 150 mg / kg (Table 4). It was found that the efficacy of acarxa in a dose of 100 mg / kg in DV is higher than the effectiveness of acetamizole in a dose of 150 mg / kg in case of ascariasis, trichocephalosis and porcine azophagostomiasis.

 Example 6

 The effectiveness of Acarx for carnivorous helminthiases.

 Three puppies infected with toxocaras were fed akars at a dose of 50 mg / kg, three 40 mg / kg at DV, three acetamizole at a dose of 50 mg / kg. After 10 days, the dogs were killed, a helminthological study of the small intestine was performed and no helminths from all doses were found. At the same time, Acars is effective at a dose of 80 mg / kg (40 mg / kg in terms of DV), i.e. at a dose lower than acetamizole recommended.

 Acarz was tested on one cat infected with dipyllidia at a dose of 80 mg / kg (40 mg / kg in terms of DV) when administered twice with an interval of 24 hours and obtained 100% effect according to coproscopy.

 Example 7

 The study of acute toxicity of Acarx.

 Akzars at a dose of 800 mg / kg DV (20-fold therapeutic dose) was tested on two sheep, two pigs and two dogs and did not reveal any side effects. The drug relates to low toxicity compounds.

 Example 8

 Evaluation of the embryotropic effect of Acarx.

Akzars was administered to 200 white female rats (165 experimental and 35 control) once, individually through the mouth in a therapeutic 80 mg / kg (40 mg-kg DV) and 3 times higher doses from 1 to 19 days of pregnancy. On day 20, all experimental rats were killed, the abdominal cavity and uterus were opened, the number of yellow bodies of pregnancy, implantation sites, and the number of live, dead, and resorbed fetuses were calculated. The embryos were carefully examined, the mass and craniocaudal dimensions were determined, the placenta was measured and weighed, the placental coefficient was determined by the formula:
placental mass, mg / fetal weight x 100
The study of the state of internal organs was carried out according to the Wilson method, and the bone system was studied according to the Dawson method.

 As a result of a study in animals that received a therapeutic and increased dose of Acarx, embryotropic properties were not detected; in those receiving a therapeutic dose of acetamizole, a 1.5% case of embryotoxic action was revealed, and from an increased dose, 7% of cases of embryotoxic and 11% of teratogenic effects, which indicates the proposed form.

 The technical and economic essence of the proposed drug and the method of treating animals with him for nematodoses, cestodoses and trematodoses in the following.

 1. The process of making Acqars is simple and technological. The drug will be manufactured in the factory.

 The components that make up the drug are manufactured by domestic industry.

 2. The combination of 2-acetiaminobenzimidazole with dextrin promotes synergism, expressed in the absence of embryotoxic and teratogenic effects of the former, which allows the drug to be used in animals without restriction, as well as a significant expansion of the complex's action on helminths at a lower therapeutic dose.

 3. The use of Acarx makes it possible to increase the number of animals processed in the country in the presence of the same amount of starting ingredients.

Claims (1)

 Anthelmintic agent based on 2-acetaminobenzimidazole, characterized in that it further contains dextrin in the following ratio of components, parts by weight 2 acetaminobenzimidazole dextrin 1.0 0.6 1.0 - 1.4.

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