RU2093045C1 - Food addition - Google Patents

Abstract

FIELD: food and pharmaceutical industry. SUBSTANCE: invention relates to enzyme-base food additions obtained from vegetable raw. Addition has an enzyme complex isolated from biomass of cultured cells of ginseng and polyscias fern-leaved, special additions and a base - an aqueous ethanol or water. Enzyme complex content is 0.1-2.5 wt.-% of a base. EFFECT: improved quality and effectiveness of addition. 2 cl

Description

 The invention relates to the food and pharmaceutical industries and relates to food additives based on enzymes derived from plant materials.

 It is known to use dry food extract or powder from ginseng root [1, 2, 3, 4] or tinctures from it [5, 6, 7] as food additives. These additives give products additional tonic and nutritional properties. However, the use of preparations from ginseng has a number of contraindications, namely hypertension, neoplasms.

 It is known to use acylated derivatives of superoxide dismutase (SOD) as preservatives for blood products [8] Microbiological SOD is used to prevent the destruction of products if the cause of their destruction is peroxide [9] SOD (human) and SOD together with catalase are used in pharmaceutical compositions for the oral cavity [ 10] Immobilized SOD is used in cosmetics [11] It should be noted that human SOD is a very expensive product, microbiological SOD is of limited use due to the high Coy allergic activity and is used only in external formulations.

 Known tincture from biomass of ginseng root tissue "Ginseng" [12] It is made on 40% ethanol with a content of 10% (1 10) dry ginseng biomass. Used in medical practice as a stimulating and tonic for asthenia, overwork, etc. Contraindicated in hypertension, irritability, insomnia. This tincture is not recommended as an additive in therapeutic nutrition.

 For food products, especially those used for therapeutic purposes, it is important to increase their biological value and improve taste. This is usually achieved by introducing various food additives into the products. It is known that for people prone or suffering from coronary heart disease, food products are enriched not only with vitamins, but also with antioxidants [13]. It is very important that food additives are obtained from environmentally friendly sources.

 The objective of the invention is the creation of food additives from natural environmentally friendly raw materials with tonic and antioxidant effects, the expansion of the arsenal of food additives for therapeutic and preventive nutrition.

The objective of the invention is realized by the proposed food supplement, which includes an enzyme complex isolated from the biomass of cultivated plant cells of aralia ginseng and polyscias, a base of aqueous ethanol or water and target additives in the following ratio of components (wt.):
Enzyme complex 0.1 2.5
Targeted supplements 0.01 2.5
Water ethanol or water else
The enzyme complex is isolated from the biomass of cultured aralia cells: ginseng (IFRJ-1, IFRZh-5, VSCKKVR N 26 strains) and fern leaf polysias (strain VSCKKVR N 24) [14] The enzyme complex includes SOD, catalase, peroxidase, its activity
superoxide dismutase: 20.0 ± 1.8 conv. opt. units D ₄₆₀ / mg protein
catalase: 0.14 ± 0.02 mm H ₂ O ₂ / mg protein,
peroxidase: 0.60 ± 0.03 standard opt.e. D ₄₃₅ / mg protein.

 The enzyme complex (FC) is stable, the activity lasts for more than a year, has low toxicity, it lacks immunotoxicity, and has high anti-inflammatory activity.

The temperature of the decomposition of the drug 85 90 ^o C
Optimum temperature 30 40 ^o C
Optimum pH 5.0 8.0
The use of such a complex in the composition of food additives is not described.

 FC can be introduced into food products in the form of a lyophilized powder, alcohol, water-alcohol or aqueous solution. The content of FC in the food additive 0.1 to 2.5 wt. To create a presentation, the necessary target additives may be added to the composition. For example, food flavors, fragrances, food colors, citric acid (if necessary to create a favorable pH).

The invention is illustrated by the following examples:
Example 1. wt.

Ethyl alcohol 96 ^o 95
FC (from pcs. IFRZh-1) 2.5
Water dist. rest
Example 2

Ethyl alcohol 96 ^o 40
FC (from pieces of VSKKKVR N 26) 1,0
Food flavoring 0.5 (e.g. lemon)
Sorbitol 2.0
Water rest
Example 3

Ethyl alcohol 96 ^o 20,0
FC (from pcs. IFRZh-5) 0.1
Food flavoring 0.5 (e.g. peppermint)
Citric acid 0.1
Xylitol 1.0
Food Coloring 0.001
Water dist. rest
Example 4

FC (from pieces of VSKKKV N 24) 1,5
Sodium Benzoate 0.01
Water dist. rest
The food supplement is prepared by a simple technology: the corresponding amount of the component is dissolved in alcohol (FC) and in water (sorbitol, citric acid, dye), i.e. in a suitable solvent, at room temperature, are combined, aged 10 12 hours, filtered.

The composition (example 1) can be used as an additive to lipid-containing products (meat, fish, butter, dough, etc.) and various drinks in the amount of 10 20 drops or 10 15 mg / kg of product. Composition 2 and 4 can be used as a tonic to any beverage: tea, coffee, juice, compote, milk, etc. Dosage 20 30 drops per 200 250 ml of the drink, which corresponds to 5 10 mg / l of the drink. The same compositions can be added to the dough at the rate of 40 50 drops per 200 g of baked product. Composition 3 can be used as an alcoholic beverage or as compositions 2 and 4. When using additives, an increase in the shelf life of products by 15 - 30% was noted
A PK-based food supplement was tested on 30 adult taster volunteers who took these supplements 3 times a day with drinks and rolls for 30 days. It was noted that supplements improved the taste of products (97%), 93% of the subjects noted after 10 14 days they felt an increase in physical and mental performance, a decrease in drowsiness and fatigue, an increase in a sense of confidence. In addition, there was an improvement in the function of the mucous membrane of the oral cavity, gums and tonsils.

 Included in the composition of the nutritional supplement FC obtained from the biomass of the Aralia plant cell culture (ginseng and polysacias) is an environmentally friendly product, since the raw materials for biomass production are completely controlled (the nutrient medium for biomass production includes micro amounts of mineral salts, sucrose, casein hydrolyzate, agar-agar), in contrast, for example, from products from ginseng root, which is grown under uncontrolled conditions. At the same time, FC contains the optimal “natural” ratio of SOD, catalase and peroxidase. Unlike products from ginseng root, it has no contraindications for use, since it does not contain glycosides, since it is released from biomass at its minimum content. FC, entering the body with food, contributes to the normalization of the content of the toxic oxygen radical, thereby ensuring the normal course of metabolic processes and the structural and functional integrity of all types of biomembranes. It exhibits the properties of a natural antioxidant that inhibits oxidative processes, therefore, a food supplement based on it, when introduced into products containing lipids, prevents their deterioration.

Thus, a dietary supplement based on an enzyme complex from biomass of cultivated aralia plant cells
will improve the biological value of products, giving them tonic and antioxidant properties,
will allow to produce therapeutic and prophylactic products, for example, for specialists whose work requires special attention and stress, working in harmful conditions,
will expand the range of organic food additives.

Literature
1. Application of France N 2671488 from 01/10/91, cl. A 61 K 35/76. Dietary or pharmaceutical preparation with anti-fatigue and anti-stress properties.

 2. The application of Canada N 1298203 from 03/31/92, cl. A 61 K 35/78. Product additives containing antioxidant extracted from plants.

 3. A.S. USSR N 1833166 from 12.13.91, cl. A 23 N 2/00. Soft drink tonic.

 4. RF patent N 2003670 dated 11/13/92, cl. C 12 C 3/06. Alcoholic cream "Ginseng".

 5. RF patent N 2003261 dated 11/30/93, cl. A 23 C 3/00. Composition for confectionery type dragees "Lesovichok".

 6. A.S. USSR N 1833167 from 12.13.91, cl. A 23 I 2/00. Non-alcoholic tonic drink.

 7. USSR N 1833166 from 12.13.91, cl. A 23 I 2/00. Non-alcoholic tonic drink.

 8. EPO N 292321 of 11.23.88 (87/122567 of 05.21.87 Japan), cl. C 12 N 9/92. Acylated derivatives of SOD and their use.

 9. Japan, application N 61-185184 from 9.01.87 (59-274299 from 08/18/86), cl. C 12 N 9/02. New SOD, microbiological production method.

 10. Japan, application N 63-132820 from 4.06.88 (61-279497), cl. A 61 K 7/28. Composition for the oral cavity.

 11. Japan, application N 61-250304 from 5.10.89 (63-80612 from 03.31.88), cl. A 61 K 7/00. Cosmetic materials for the skin.

 12.M.D. Mashkovsky. Medicines M. Medicine. Part 1, p. 162.

 13. Vitamins antioxidants, free radicals and coronary heart disease. Dythic Garry G. // Brit. Food J. 1990. -92, N 8 C.32-3.

 14. RF patent N 2046140 from 07/28/93, cl. A 61 K 35/78. A method for isolating an enzyme complex from cultured plant cells.

Claims (1)

 1. A food supplement comprising an active product from the biomass of cultivated plant cells of Aralia and a base, characterized in that the active product contains an enzyme complex isolated from the biomass of cultivated plant cells of ginseng and polystyrosis fernica, and as a base contains aqueous ethanol or water at the following ratio of components, wt. Enzyme complex 0.1 2.5
Water ethanol or water Else
2. The nutritional supplement according to claim 1, characterized in that it further comprises target additives, for example, food flavorings, fragrances, food colors, citric acid and sweeteners.