JP3241610B2 - Pharmaceuticals containing bean tea extract as an active ingredient, and foods and cosmetics containing the same - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a medicament comprising a tea extract as an active ingredient, and more particularly to a medicament having anti-inflammatory, anti-allergic and hyaluronic acid decomposition inhibitory activities, and food and cosmetics containing the same. About.

[0002]

2. Description of the Related Art With the development of science and technology, many compounds have been synthesized, and drugs having various actions have been found.
For example, a large number of drugs have been developed mainly as chemically synthesized products as anti-inflammatory agents. The point of action of aspirin, which has been used clinically since about 100 years ago, lies in the inhibition of cyclooxygenase, the first enzyme in the process from arachidonic acid to the synthesis of prostaglandin (hereinafter abbreviated as "PG"). As a result, e.g., indomethacin, which has a strong pharmacological effect by a similar mechanism of action, has been developed.

[0003] Further, as an antiallergic agent, a chemically synthesized product such as disodium chromoglycate (hereinafter abbreviated as "DSCG") and caffeic acid as an inhibitor of 5-lipoxygenase which generates leukotriene from arachidonic acid are used. A mugwort extract containing a large amount is used.

[0004] The above all relate to the components of pharmaceuticals. On the other hand, food materials which are thought to contribute to anti-inflammatory or anti-allergy are also required. That is the current situation. In addition, various cosmetic raw materials having anti-inflammatory or anti-allergic activity have been searched for, but safe and promising ones have not yet been obtained.

On the other hand, hyaluronic acid derived from microorganisms or the like is mainly used in cosmetics as a humectant, but recently, the inhibitory activity against hyaluronidase, a hyaluronic acid-degrading enzyme, is used as an index to evaluate the antioxidant properties of tea extract. Attempts have been made to evaluate allergic activity (Yumie Maeda et al .: Food and Essays, Vol. 31, pp. 233-237, 199).
0), a correlation between hyaluronidase inhibitory activity and antiallergic activity has been suggested.

[0006]

For the 21st century, the necessity of prevention rather than treatment of disease has been called for. For example, inflammation and allergies are considered to be an overreaction of the human body's resistance, but some precautionary measures are needed when suffering from onset. The recent surge in allergic diseases, such as hay fever, is only remarkable. Originally, IgE, which is present in the human body for the purpose of eliminating parasites, has recently been working on those who cause allergies along with a decrease in the number of parasites in the human body, but fats, especially linoleic acid, are used as food. We cannot overlook the fact that the intake of those that contain a lot is increasing. Linoleic acid is easily desaturated in vivo into arachidonic acid, and the resulting leukotriene causes allergy. As society becomes richer, allergies tend to increase due to a decrease in the body's possession of parasites and an increase in fat intake, but no effective antiallergic drugs have been found. Development is an important issue.

On the other hand, the necessity of maintaining and increasing the hyaluronic acid content of a living body is not limited to the problem of skin alone. In the aorta and joint cavity fluid, the water retention structure by hyaluronic acid plays an important role. As aging is accompanied by a decrease in the hyaluronic acid content of the human body,
For an aging society, the need to maintain the moisture content, and thus the flexibility, retained by hyaluronic acid, such as skin and blood vessels, is expected to increase more and more. At present, attention is focused only on hyaluronic acid used externally as a humectant for cosmetics, and there are almost no attempts to maintain the hyaluronic acid content and, consequently, the moisture content in the human body.

[0008] The above-mentioned problem can be solved by administration of a drug, but it is more preferable that the problem is solved by a substance which is taken or used daily, such as food or cosmetics. However, up to now, promising food materials and cosmetic raw materials having anti-inflammatory, anti-allergic and anti-hyaluronidase activities have not been obtained, and provision of such raw materials and raw materials remains as an issue.

[0009]

Means for Solving the Problems In order to solve the above-mentioned problems, the present inventors have proposed the use of both enzymes, cyclooxygenase, which is the first enzyme in the process from arachidonic acid to PG synthesis, and hyaluronidase, which is a hydrolase of hyaluronic acid. Intensive search was conducted to find a natural product that inhibits and also has antihistamine activity. Here, the reason why the inhibition of hyaluronidase was aimed at is that inhibition of this enzyme strongly correlates with not only maintenance of hyaluronic acid levels in the human body but also anti-inflammatory and anti-allergic activities. This enzyme is activated during inflammation, destroying the matrix of connective tissue,
It is thought that this enzyme plays a role in increasing the permeability of cells and blood vessels of the inflammatory system, so that inhibition of this enzyme is considered to lead to anti-inflammatory. Hyaluronidase is also likely to be involved in a series of processes in mast cells, starting from binding of the IgE-antigen complex to the receptor to release of histamine granules (degranulation). Large morphological changes, such as degranulation, cannot occur without the temporary disruption of the water retention structure by hyaluronic acid. In fact, many antiallergic agents have a hyaluronidase inhibitory activity.

As a result of searching for a natural product having the above-mentioned properties, a substance having the above three activities in the extract was found in the tea extract-based solvent extract, and the present invention was completed.

[0011] That is, a first object of the present invention is to provide a medicament selected from an anti-inflammatory agent, an anti-allergic agent and a hyaluronic acid decomposition inhibitor, which comprises a tea extract as an active ingredient. Another object of the present invention is to provide foods and cosmetics containing the medicine.

The tea tea extract used in the present invention can be obtained, for example, by extracting tea tea with an aqueous solvent. The raw material of tea (Rubaceae perennial shrub, Rubus suavissims
S.Lee) has been used as sweet tea in China since ancient times. This tea can be subjected to extraction using leaves or stems, particularly leaves obtained by drying the leaves in the sun, as raw materials. In addition, this tea is a sweet tea of the family Saxifragaceae [ Hydrangea macrophylla Seringe var.
thunbergii Makino ( Saxifragaceae : Saxifragaceae)] is a completely different plant.

The aqueous solvent used for the extraction may be water alone or any mixture of water and one or two polar solvents such as lower alcohols such as methanol and ethanol, and acetone. However, since the active ingredient of the present invention cannot be efficiently extracted with only a polar solvent, it is desirable to always use a mixed solution with water and the mixing ratio of the solvent is 90% or less. Among these solvents, in view of the fact that the extract is finally incorporated into foods and the like, it is preferable to use water, ethanol, or a mixture thereof from the viewpoint of safety.

The ratio of bean tea to the solvent at the time of extraction is not particularly limited either.
000 times by weight, especially 5-100 times by weight in terms of extraction operation and efficiency. The extraction temperature is conveniently in the range of room temperature to the boiling point of the solvent under normal pressure, and the extraction time is preferably in the range of 10 minutes to 24 hours.

[0015] Any of the thus obtained bean tea-based solvent extract as it is, a concentrate thereof, and a dried product obtained by removing the solvent from the eluate can be used. It is preferable that the organic solvent is in a dry state in terms of safety.

The medicament of the present invention is prepared by preparing a tea extract as it is or with a known pharmaceutical carrier. The medicament of the present invention includes oral preparations such as tablets, granules, powders and syrups; parenteral preparations such as suppositories and external preparations; candy, troches, gums, yogurt, ice cream, puddings, jellies, mizuyokan , Coffee beverages, juices, carbonated beverages, soft drinks, milk, whey beverages, lactic acid bacteria beverages and other dosage forms, lotions, cosmetic creams, emulsions, foundations, lipsticks, hair styling, hair tonics, It can be in the form of a formulation to be incorporated into cosmetics such as hair restorations, toothpastes, mouthwashes, shampoos, rinses, bath additives and the like.

The pharmaceutical carrier that can be used in the preparation of the medicament of the present invention is not particularly limited, and those usually used can be used.
Solid carriers such as starch, lactose, sucrose, mannitol, carboxymethylcellulose, corn starch and inorganic salts; distilled water, physiological saline, aqueous glucose solutions, alcohols such as ethanol, liquid carriers such as propylene glycol and polyethylene glycol; various animal and vegetable oils And oily carriers such as white petrolatum, paraffin and waxes.

In order to produce the above-mentioned foods, cosmetics, toothpastes, shampoos and the like using the medicament of the present invention, appropriate components usually used depending on the type of the product can be blended. For example, when preparing food,
Glucose, fructose, sucrose, maltose, sorbitol,
Stevioside, rubusoside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L
-Ascorbic acid, dl-α-tocopherol, sodium erysorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, carrageenan, casein, gelatin , Pectin, agar, vitamin B, nicotinamide, calcium pantothenate, amino acids, calcium salts, pigment,
It can be produced by appropriately blending those used as ordinary food ingredients such as flavors and preservatives.

Further, when preparing cosmetics, oils and fats such as vegetable oils, waxes such as lanolin and beeswax, hydrocarbons, fatty acids, higher alcohols, esters, various surfactants, pigments, fragrances, It can be produced by appropriately blending those commonly used as raw materials for cosmetics, such as vitamins, plant / animal extract components, ultraviolet absorbers, antioxidants, preservatives and bactericides.

In the case of preparing cosmetics and the like using the tea tea extract of the present invention, other anti-inflammatory and anti-allergic cosmetic raw materials, for example, licorice extract component (particularly glycyrrhizic acid),
Diphenhydramine hydrochloride, azulene, dl-alpha-tocopherol and its derivatives, the use of a vitamin B ₂ and B _6, it is possible to enhance the effect. Since the bean tea extract strongly inhibits hyaluronidase, a hyaluronic acid-degrading enzyme, its use in combination with hyaluronic acid can further enhance its moisturizing effect. The tea tea extract alone has a moisturizing / beautifying skin effect indirectly by inhibiting the decomposition of hyaluronic acid in the skin, but other moisturizing / beautifying cosmetic ingredients such as elastin, collagen, lecithin, squalene and placenta. Liquid (placental extract), glycerin, glycols, fermentation metabolite, lactic acid bacteria culture, vitamin A and C, sodium chondroitin sulfate, sodium 2-pyrrolidone-5-carboxylate (PCA-Na), Bacmondou mucus polysaccharide, etc. It is desirable to further enhance the effect when used together with a plant polysaccharide or the like.

Sweet tea has been used as sweet tea in China since ancient times, and the extract used in the present invention has no problem in terms of safety. However, it is desirable that the amount of the extract of bean tea in the medicament of the present invention be in the concentration range of 0.01 to 5.0% in terms of dry weight in terms of the effect, the scent when added, and the color tone.

[0022]

The cyclooxygenase inhibitory activity of the extract of bean tea, which is the active ingredient of the medicament of the present invention, ie, the anti-inflammatory activity by inhibiting PG synthesis is about 1/5 of that of aspirin, and the antihistamine activity is anti-allergic. It was comparable to that of DSCG, the active ingredient of the drug Intal.
Therefore, the medicament of the present invention is useful as a medicament having an anti-inflammatory action and an anti-allergic action. Also,
By ingesting foods containing bean tea extract on a daily basis,
It can prevent and improve inflammation, sore throat, hay fever, cough, etc. associated with a cold. Similarly, it is possible to improve skin itchiness and the like by using a cosmetic containing the tea extract.

The bean tea extract according to the present invention is a powerful inhibitor of the degradation of hyaluronic acid and suppresses a decrease in the content of hyaluronic acid contained in skin, arterial walls, joint cavities and the like. Although this is an indirect effect, it enhances the moisturizing property and flexibility of the skin, prevents arteriosclerosis associated with aging, and contributes to improvement of arthritis.

The bean tea extract is a plant extract whose safety has been established, and has various effects as a hyaluronic acid degradation inhibitor in addition to its action as an anti-inflammatory and anti-allergic agent. , Its versatility is unparalleled.

[0025]

Examples Next, examples relating to a method for producing a tea tea extract, a cyclooxygenase inhibition test, a hyaluronidase inhibition test, a histamine release inhibition test, and production of an anti-inflammatory agent, an anti-allergic agent and a hyaluronic acid degradation inhibitor will be described.
The present invention will be described in more detail. Example 1 Production of a tea extract: 100 g of tea was placed in a 3000 ml Erlenmeyer flask, added with 1000 ml of hot water, and extracted in a boiling water bath for 3 hours. This was filtered, and the obtained filtrate was freeze-dried to obtain 32.3 g of an extract (Tengcha extract 1).

Example 2 Production of a tea extract: 100 g of tea is placed in a 3000 ml Erlenmeyer flask, and 50% by volume of ethanol 1000 m
l, and the mixture was gently stirred every hour at room temperature and extracted for 24 hours. This was filtered, the obtained filtrate was concentrated under reduced pressure to remove ethanol, water was added, and the mixture was freeze-dried to obtain 31.1 g of an extract (Tengcha extract 2).

Example 3 Assay of cyclooxygenase inhibitory activity: The extract obtained in Example 1 was measured for its cyclooxygenase inhibitory activity by the following method. Table 1 shows the results.

(Measurement Method) The inhibitory effect of the microsomal of sheep seminal vesicle gland (Funakoshi Pharmaceutical Co., manufacturer code A3) on the cyclooxygenase activity was measured by an oxygen absorption method. That is, in a 4 ml reactor equipped with an oxygen electrode,
3.835 ml of 0.2 M Tris-HCl buffer (pH 8.0) containing 10 mM L-tryptophan, 25 μl of 40 μM hemoglobin solution, 30 μl of the above buffer containing 100 μl of test sample solution and 0.6 mg of microsome were added, and 5
After preincubation for 10 minutes, the reaction was started by adding 10 μl of a 10 mM arachidonic acid solution in ethanol. The reaction temperature is 30 ° C. The inhibition rate I (%) was determined by the following formula, where C was the initial enzyme absorption rate of the control to which purified water was added instead of the test sample solution, and S was that at the time of addition of the test sample. I (%) = 100 × (C−S) / C In both the tea tea and the extract of Arhat fruit, the dose-dependent curve of the inhibitory activity did not show linearity, and became S-shaped with an inflection point around I = 50%. Was.

[0029]

[Table 1]

Example 4 Assay of hyaluronidase inhibitory activity: The extract obtained in Example 1 was measured for its hyaluronidase inhibitory activity by the following method. Table 2 shows the results.

(Measurement Method) Using hyaluronidase (Sigma, Type IV) derived from bovine testes, the inhibitory effect of compound 48/80 on the activation stage of the inactive enzyme was measured mainly. Enzyme activity, an increase in the reducing power of tetrasaccharide to the N- acetylhexosamine produced by hydrolysis of hyaluronic acid and the reducing end by colorimetry at A _585, was determined (Yumi Maeda Megumira: ShokuMamorushi ,
31, 233-237, 1990). That is, an appropriate amount of a test sample was added to 100 μl of a 0.1 M acetate buffer (pH 4.0).
Was added to the test tube, 0.10 mg (100 NF units) of the enzyme dissolved in 50 μl of the same buffer was added, and the mixture was incubated at 37 ° C. for 20 minutes. Then, the compound 48/80 (5
0 μg) and further incubate at 37 ° C. for 20 minutes. Finally, sodium hyaluronate (200 µg, derived from a microorganism) dissolved in 250 µl of the same buffer was added, and the mixture was incubated at 37 ° C for 40 minutes.
After adding 100 μl of NaOH and cooling with ice, add 100 μl of borate buffer (pH 9.1) and boil for 3 minutes. After cooling on ice, p-dimethylaminobenzaldehyde TS 3m
After incubating at 37 ° C. for 20 minutes,
₅₈₅ was measured. As a control, the above acetate buffer was used instead of the sample solution. Further, as each blank, the above acetate buffer was used instead of the enzyme solution. The inhibitory activity was represented by the inhibition rate determined from the following equation. A: A ₅₈₅ B control solution: A ₅₈₅ of control solution blanks C: A ₅₈₅ D of the sample solution: the blank of the sample solution A ₅₈₅

[0032]

[Table 2] * Disodium chromoglycate (product name: Intal)

Example 5 Test for Inhibiting Histamine Release from Mast Cells: The extract obtained in Example 1 was assayed for its histamine release inhibitory activity by the following method (Yuko Hirai et al .: Pharmaceutical Pharmaceutical Magazine, 37).
374-380, 1983). Table 3 shows the results.

(Measurement method) Bovine serum albumin (BS) was collected from peritoneal cells collected by injecting Tyrode solution into the abdominal cavity of Wistar rats that had been bled to death.
A)-Mast cells were isolated by a double-layer centrifugation method using physiological saline (specific gravity: 1.068). The cells obtained were 2 × 10
^The cells were suspended in Tyrode's solution containing 0.1% BSA at a concentration of ⁶ cells / ml to prepare a cell suspension. Sample solution 10μl
After adding 10 μl of the cell suspension to the mixture and allowing it to stand at 37 ° C. for 10 minutes, Compound 48/80 was used as a degranulation-inducing agent.
20 μl (5 μg / ml) was added and reacted at 37 ° C. for 10 minutes. Thereafter, the mixture was cooled on ice once and centrifuged (15
(0 × g, 5 minutes), the amount of histamine released in the supernatant was measured by high performance liquid chromatography with a fluorescence detector. The histamine release inhibitory activity was calculated by the following equation. Histamine release inhibition rate (%) = 100 × [1- (SR−
C) / (RC)] C: The amount of histamine released from control cells R: The amount of histamine released from cells when an inducer is added SR: When the inducer is added in the presence of a sample Histamine release from cells

[0035]

[Table 3] * Disodium chromoglycate (product name: Intal)

Example 6 Tablets: 150 g of bean tea extract 1 was mixed with the same amounts of lactose and magnesium stearate (5 g), and the mixture was tableted with a single-shot tableting machine to a diameter of 10 mm and a weight of 300 mg.
Tablets were produced.

Example 7 Condyle Granules: The tablets obtained in Example 6 were pulverized, sized, and sieved to obtain 20-50 mesh granules.

Example 8 Candy: (composition) (weight part) Powdered sorbitol 99.7 Perfume 0.2 Teng tea extract 0.05 Sorbitol seed 0.05 Total amount 100

Example 9 Candy: (composition) (weight) sugar 47.0 water syrup 49.76 fragrance 1.0 water 2.0 sweet tea extract 0.24 total amount 100

Example 10 Lozenges (composition) (weight) Gum arabic 6 Glucose 73 Tengcha extract 0.05 Potassium potassium phosphate 0.2 Potassium potassium phosphate 0.1 Lactose 17 Fragrance 0.1 Magnesium stearate Remaining amount 100

EXAMPLE 11 GAM: (Composition) (weight part) Gum base 20 Calcium carbonate 2 Stevioside 0.1 Teng tea extract 0.05 Lactose 76.85 Flavor 1 Total amount 100

Example 12 Chewing Gum: (Composition) (Weight) Gum Base 20.0 Sugar 75.62 Flavor 1.0 Citric Acid 1.0 Water 2.0 Tian Tea Extract 0.38 Total 100

Example 13 Caramel: (composition) (weight part) Granulated sugar 32.0 Water candy 20.0 Powdered milk 40.0 Hardened oil 4.0 Food salt 0.6 Flavor 0.02 Water 3.22 Tengcha extract 0.16 Total amount 100

Example 14 Coffee Jelly: (Composition) (weight part) Granulated sugar 15.0 Gelatin 1.0 Coffee extract 5.0 Water 78.93 Teng tea extract 0.07 Total amount 100

Example 15 Ice cream: (composition) (weight) fresh cream (45% fat) 33.8 skim milk powder 11.0 granulated sugar 14.8 sweetened egg yolk 0.3 vanilla extract 0.1 water 39.93 Tengcha extract 0.07 Total amount 100

Example 16 Custard Pudding: (Composition) (Weight) Milk 47.51 Whole Egg 31.9 Upper Sugar 17.1 Water 3.4 Teng Tea Extract 0.09 Total 100

Example 17 Mizuyokan: (Composition) (Weight) Akasei bean 24.8 Powder agar 0.3 Dietary salt 0.1 Kamikasu 24.9 Teng tea extract 0.1 Water 49. 8 Total amount 100

Example 18 Juice: (composition) (weight part) Frozen-concentrated unshu mandarin orange juice 5 fructose-glucose liquid sugar 11 citric acid 0.2 L-ascorbic acid 0.02 sweet tea extract 0.05 flavoring 0 .2 Color element 0.1 Total amount of water remaining 100

Example 19 Carbonated Drink: (Composition) (Weight) Granulated Sugar 8.0 Concentrated Lemon Juice 1.0 L-Ascorbic Acid 0.10 Citric Acid 0.06 Sodium Citrate 0.05 Coloring Ingredients 0.05 Perfume 0.15 Carbonated water 90.55 Tengcha extract 0.04 Total amount 100

EXAMPLE 20 Lactic Acid Bacteria Beverage: (Composition) (Weight) 21% Milk Solids Fermented Milk 14.76 Fructose Dextrose Liquid Sugar 13.31 Peptin 0.5 Cuenoic Acid 0.08 Flavor 0.15 Water 71.14 Tengcha extract 0.06 Total 100

EXAMPLE 21 Coffee Beverage: (Composition) (Weight) Granulated Sugar 8.0 Skim Powdered Powder 5.0 Caramel 0.2 Coffee Extract 2.0 Flavor 0.1 Poly Glycerin 0.05 Fatty acid ester Dietary salt 0.05 Water 84.56 Tea extract 0.04 Total amount 100

Example 22 Dentifrice: (Composition) (Weight) Calcium Diphosphate 42 Glycerin 18 Carrageenan 0.9 Sodium Lauryl Sulfate 1.2 Sodium Saccharin 0.09 Butyl Paraoxybenzoate 0.005 Tea Tea Extract 0 .05 Perfume 1 Water balance 100

Example 23 Mouthwash: (composition) (weight part) Sodium lauryl sulfate 0.8 glycerin 7 sorbitol 5 ethyl alcohol 15 Tengcha extract 0.05 1-menthol 0.05 05 Fragrance 0.04 Saccharin sodium 0.1 Water remaining amount Total amount 100

Example 24 Softening lotion (weakly acidic): (Composition) (weight part) Glycerin 5.0 Propylene glycol 4.0 Sodium hyaluronate 0.1 Teng tea extract 0.05 Polyoxyethylene sorbitan monolaurin Acid ester 1.5 (20 EO) Polyoxyethylene lauryl 0.5 ether (20 EO) Ethanol 10.0 Flavor 0.1 Dyeing dye proper amount Preservative proper amount UV absorber proper amount Refining Water 78.75

Example 25 Emollient Cream: (Composition) (Weight) Beeswax 2.0 Stearyl Alcohol 5.0 Stearic Acid 8.0 Squalane 10.0 Self-Emulsifying Propylene Glycol 3.0 Monostearate Polyoxy Ethylene cetyl ether 1.0 (20EO) Fragrance 0.5 Preservatives Qs Antioxidant Qs Propylene glycol 7.8 Glycerin 4.0 Sodium hyaluronate 0.1 Teng tea extract 0.1 Triethanolamine 1.0 Refined water 57.5

Example 26 Emollient lotion: (composition) (weight) stearic acid 2.0 cetanol 1.5 petrolatum 3.0 lanolin alcohol 2.0 liquid paraffin 10.0 polyoxyethylene monooleic acid 2. 0 Ester (10EO) Fragrance 0.5 Antioxidant Appropriate amount Preservative Appropriate amount Propylene glycol 4.8 Glycerin 3.0 Sodium hyaluronate 0.1 Teng tea extract 0.1 Triethanolamine 1.0 Refined water 70.0

Example 27 Emulsion foundation: (composition) (weight) stearic acid 2.4 propylene monostearate 2.0 glycol setosteryl alcohol 0.2 liquid lanolin 2.0 liquid paraffin 3.0 myristin Isopropyl acid 8.5 8.5 Propyl parahydroxybenzoate qs Refined water 64.1 sodium carboxymethylcellulose 0.2 bentonite 0.5 propylene glycol 3.8 sodium hyaluronate 0.1 bean tea extract 0.1 triethanolamine 1.1 Methyl parahydroxybenzoate qs Titanium oxide 8.0 talc 4.0 color pigment qs perfume qs

Example 28 Hair Restoration Hair Tonic: (Composition) (Weight) Ethanol 70.0 Assembly Extract 0.2 Vitamin E 0.2 L-Menthol 0.4 Glycyrrhizin 0.1 Teng Tea Extract 0.1 Salicylic acid 0.5 Glycerin 0.5 Perfume Appropriate amount Purified water 28.0

EXAMPLE 29 Shampoo: (Composition) (Weight) Alkyl ether sulfate 16.0 Sodium (AES-Na) Lauric acid diethanolamide 4.0 Propylene glycol 1.9 Teng tea extract 0.1 Preservative , Pigment, fragrance Appropriate amount Purified water 78.0

Example 30 Rinse: (composition) (weight part) Stearyl dimethyl chloride 1.4 Benzyl ammonium stearyl alcohol 0.6 Glycerin monostearate 1.5 Food salt 0.1 Teng tea extract 0.1 Purified Water production 96.3 or more

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI A23G 9/00 A23G 9/00 A23L 1/06 A23L 1/06 1/187 1/187 1/30 1/30 B 2/38 2/38 C 2/52 A61K 7/00 K A61K 7/00 W 7/02 P 7/02 7/06 7/06 7/075 7/075 7/08 7/08 7/26 7/26 7 / 48 7/48 7/50 7/50 9/20 9/20 A61P 17/00 A61P 17/00 37/08 37/08 43/00 113 43/00 113 A23L 2/00 F Int.Cl. ⁷ , DB name) A61K 35/78 A23C 9/13 A23G 3/00 A23G 9/00 A23L 1/06 A23L 2/38 A23L 2/52 A61K 7/00 A61K 9/20 BIOSIS (DIALOG) CA (STN) MEDLINE (STN)

Claims (8)

(57) [Claims] 1. A hyaluronic acid decomposition inhibitor comprising a tea extract as an active ingredient. 2. The hyaluronic acid decomposition inhibitor according to claim 1, wherein the bean tea extract is an extract using a mixed solvent of water and a polar solvent. 3. The extract of bean tea from 0.01 to 0.01% by dry weight.
The hyaluronic acid decomposition inhibitor according to claim 1 or 2, which contains 5%. 4. The hyaluronic acid decomposition inhibitor according to any one of claims 1 to 3, which is a lozenge. 5. The food additive according to claim 1, which is a food additive.
Item 4. The hyaluronic acid degradation inhibitor according to any one of Items 3 to 3. 6. The food to be added is a group consisting of candy, troche, gum, yogurt, ice cream, pudding, jelly, mizuyokan, coffee drink, juice, carbonated drink, soft drink, milk, whey drink and lactic acid drink. The hyaluronic acid degradation according to claim 5, which is selected from the group consisting of:
Inhibitor . 7. A cosmetic comprising the hyaluronic acid degradation inhibitor according to any one of claims 1 to 3. 8. The method according to claim 1, which is selected from the group consisting of a lotion, a cosmetic cream, a milky lotion, a foundation, a lipstick, a hairdressing agent, a hair tonic, a hair growth agent, a toothpaste, a mouthwash, a shampoo, a rinse and a bath salt. Item 8. The cosmetic according to item 7.