RU2054292C1 - Method of preparing biologically active substances from velvet antlers - Google Patents

Abstract

FIELD: food and medicinal industries. SUBSTANCE: crude velvet antlers were peeled of skin, frozen, milled, dried under lyophilic conditions and ground up to powder-like state. Then bioactive substances were extracted with acidified water (pH 2.0-6.5), then ethanol is added up to concentration 30-60% and prepared mixture is kept at temperature from 4 to -20 C. Then mixture is separated for liquid fraction - spirituous extract of bioactive substances and precipitate. Alcohol is removed from the precipitate and dried, and food addition is obtained. EFFECT: improved method of bioactive material preparing. 2 tbl

Description

 The invention relates to the processing of reindeer herding products, in particular to a method for producing biologically active antler powder, extract and sediment from raw antlers, which can be used in the microbiological industry as stimulants for the growth of microorganisms, in the biochemical industry as a feedstock for various hormones and biologically active peptides in the food industry as flavoring, mineral and vitamin supplements, and the medical industry as Uhlir preparations.

 There is a method of producing an antler extract, which involves fusing the antlers of a heat-dried oven, cleansing them of the skin, crushing, extracting them three times with a water-alcohol solution, acidified with acetic acid, filtering, precipitating proteins by freezing in the cold, followed by separation of the precipitate formed.

The disadvantages of the known method for producing antler biologically active extract are:
the use of dried antlers of heat drying, losing during this process a number of thermolabile biologically active substances, such as hormones and enzymes;
the need for the process of cheating, which also leads to a decrease in the level of thermolabile biologically active substances;
the duration of the process of obtaining a biologically active extract.

 The aim of the invention is to eliminate the above disadvantages by using raw antlers as raw materials.

The essence of the method lies in the fact that after cutting, the antlers are cleaned from the skin, frozen, crushed, lyophilized, triturated to a powder state, the biologically active substances are extracted from the powder (BAS-1) with water acidified to pH 2.0-6.5 was added to the extract to a concentration of ethanol of 30-60% the resulting mixture was kept at a temperature of from 4 to -20 ^{° C,} and then the mixture is fractionated to yield a manner alcoholic extract BAS-1A and precipitate.

EXAMPLE EXAMPLE 1 1 kg of peeled skin antlers raw frozen to a temperature of minus 3 ° ^C, crushed into pieces measuring 0.5 x 1 cm and then freeze-dried. Lyophilized crushed antlers are ground to a powder state.

 The characteristics of the obtained antler powder BAV-1 are presented in table. 1.

EXAMPLE Example 2 1 kg of peeled skin antlers raw frozen to a temperature of minus 10 ^{° C,} milled to a stuffing state with 0.25 L of cold water, and then freeze vysushivayut.Liofilno dried minced beef comminuted ground to a fine powder .

 The characteristics of the obtained antler powder BAV-1 are presented in table. 1.

EXAMPLE Example 3. All the operations for obtaining antler powder is carried out in accordance with Example 1 and then dry antler powder homogenized in 100 ml chilled to a temperature of 4 ^{° C} distilled water, acidified with acetic acid to pH 2. The homogenate at constant stirring introduced 2 liters of chilled acidified water, stirring was continued for 15 min, after which the extract is introduced, cooled to minus 3 ° ^C ethanol. Ethanol is introduced to a concentration of 30% at a rate of 10 ml / h. After the introduction of ethanol, stirring was continued for 8 hours, and then allowed to extract for 16 hours to sediment at 4 ^C. The supernatant extract was fractionated alcoholic extract BAS-1A and the precipitate, which after removal of the alcohol it is dried, thus obtaining feed additive.

 The characteristics of the obtained aqueous-alcoholic extract and the dried precipitate are presented in table. 1 and 2.

EXAMPLE EXAMPLE 4 All the operations for obtaining antler powder is carried out in accordance with Example 2, after which dry antler powder homogenized in 250 ml chilled to a temperature of 10 ^{° C} distilled water, acidified with hydrochloric acid to pH 6.5. 4 l of chilled acidified water is introduced into the homogenate with constant stirring, stirring is continued for 15 minutes, and then ethanol cooled to minus 15 ^° C is introduced into the extract at a rate of 600 ml / h. The final ethanol concentration was 60% ethanol After conducting the stirring was continued for 36 hours and then allowed to extract for 28 hours to sediment at minus 20 ^C. The pooled extract was fractionated alcoholic extract BAS-1A and the precipitate, which after removal of alcohol is dried, thus obtaining a feed additive.

 The characteristics of the obtained aqueous-alcoholic extract and the dried precipitate are presented in table. 1 and 2.

Claims (1)

A METHOD FOR PRODUCING BIOLOGICALLY ACTIVE SUBSTANCES FROM PANTS, comprising grinding antlers, extracting biologically active substances with an extractant, removing insoluble substances from an extract, characterized in that the antlers are frozen before grinding, and dried after grinding, they are ground to a powdery state, they are used as an extractant 2.0 - 6.5 water, ethanol is introduced into the extract to a 30-60% concentration, the resulting mixture is kept at (+ 4) - (- 20) ^o С, then it is separated into a liquid fraction - alcohol e extract of biologically active substances and sediment, alcohol is removed from the latter and dried, thereby obtaining a feed additive.

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