RU2031114C1 - Morphological compact-container - Google Patents

Abstract

FIELD: biotechnology. SUBSTANCE: morphological compact-container consists of frame, clamp, body and retainer. Lower working surface of frame of frame has one hole corresponding to sizes and shape of cell and its upper wall has two holes for adequate contact in the process of treatment of morphological material. EFFECT: provision of reliable contact between morphological material and chemical reagents in course of treatment. 5 dwg

Description

 The invention relates to medicine and can be used in morphology. The morphological compact container is intended for the processing and storage of morphological material obtained by endoscopic and surgical procedures.

 Currently, the urgent problem is the development of a standardized methodology for processing biopsy material. According to the generally accepted methodology, biopsies obtained from various organs by endoscopic and surgical procedures are placed in a gauze bag for further processing. It should be noted that the biopsy samples are not marked, and in the accompanying registration form for sending the biopsy material to the morphological laboratory, only the name of the patient and the organ that underwent biopsy are indicated. In the laboratory, a bag with biopsy material, according to the generally accepted processing methodology, is sequentially placed in special chemical reagents, after which an individual paraffin block is placed for each biopsy. Blocks are formed by pouring molten paraffin into two L-shaped metal staples folded in a certain way, which are placed on a metal plate. Before pouring paraffin, only one biopsy specimen is placed on this plate. The resulting block is microtominated, while several sections are prepared from this biopsy specimen, each of which is fixed on a separate glass slide. The resulting preparations are stained and sent to a histologist for morphological examination.

 On average, 5 slices are prepared from one biopsy sample, for which 5 slides are needed. For example, if 5 biopsy specimens were collected from one patient, then 25 microscopic slides are required for them. Assuming that biopsies were performed on 10 patients in one day, it is easy to calculate that 250 slides are needed to process all the material obtained.

The negative sides of this technique are:
- routine of the technique;
- lack of biopsy labeling;
- high consumption of paraffin in connection with the need to prepare an individual paraffin block for each biopsy sample;
- the complexity of the process, due to the need for microtomination of each biopsy separately;
- high consumption of slides in connection with the need to fix each slice on a separate slide;
- high consumption of dyes when staining the resulting preparations.

 Naturally, such a routine and far from economical methodology for processing morphological material cannot satisfy the increasing demands of modern morphology. Such a situation dictates the need for a comprehensive search in the direction of creating special designs for the development of new, more advanced techniques, devoid of these shortcomings.

 The closest in technical essence to the claimed device is selected as a prototype gastrobiopsy cassette-container.

 The proposed methodology for processing morphological material provides immediately after biopsy to lay in the cassette-container all biopsy samples obtained. The morphological material inside the container for processing, sequentially placed in special chemical reagents (according to generally accepted methods).

 An intermediate result of this process is the formation of a paraffin polybioptate block, where biopsy samples are initially located on its surface. Subsequently, the block is subjected to microtomation. The resulting polybiopsy histological preparations after staining are sent for morphological examination.

 This design, despite a number of positive aspects, has not found application in practical healthcare, which is associated with structural flaws of the device, the most important of which is the limited access of chemical reagents to the morphological material inside the structure at all stages of chemical processing.

 The aim of the invention is to eliminate the disadvantages of the prototype, i.e. creating a design that provides full contact of the morphological material with chemical reagents during its processing.

 The goal is that the morphological compact container contains: a frame, a bracket, a housing, a retainer and is characterized in that the lower working surface of the frame has one hole corresponding to the size and shape of the cell, and its upper wall has two holes for free penetration of chemical reagents to morphological material during processing.

 Figure 1 presents the morphological compact container; figure 2 - frame; figure 3 - bracket; figure 4 - body; figure 5 - latch.

 The frame (figure 2) is a hollow rectangular structure having four walls: the lower 1, upper 2 and two side 3. The bottom wall 1 of the frame (work surface) is a rectangular plate, on the surface of which there are 5 cells 1b, recesses in two rows square shape and one hole 1A, corresponding to the size and shape of the cells 1b. Cells 1b and hole 1a are separated by horizontal and vertical jumpers towering above the surface of the lower wall 1.

 The upper wall 2 of the frame has a rectangular shape, the dimensions of which slightly exceed the dimensions of the lower wall 1. On the upper wall 2 of the frame there are two rectangular holes 2a, which are located at different angles of the plate diagonally.

 The side walls 3 of the frame are plates with a smooth surface, connecting the lower 1 and upper 2 walls of the frame, approaching the rectangles in shape.

 The bracket (Fig. 3) is a hollow rectangular structure open at the top, consisting of three walls: the bottom 1 and two side walls 2, 3. The bottom surface 1 of the bracket is represented by a smooth rectangular plate, the dimensions of which correspond to the outer dimensions of the bottom wall 1 of the frame (Fig. 1 )

 The side walls 2, 3 of the bracket (figure 3) are L-shaped. The lower vertical segments 2 are slightly smaller than the side walls 3 of the frame (figure 2). The upper horizontal segments of the side walls 3 of the bracket (Fig. 3) are turned outward and are rectangular plates with a smooth surface of small size.

 The housing (figure 4) is a rectangular bath, the dimensions of the base of which correspond to the outer dimensions of the bottom wall 1 of the bracket (figure 3).

 Vertical front 2, rear 3 and two lateral 4 walls of the housing (Fig. 4) in height correspond to the lower vertical segments of the side walls of the bracket (Fig.3).

 The latch (figure 5) is a hollow structure consisting of four walls: lower 1, upper 2, front 3 and rear 4. The lower 1 and upper 2 walls of the latch are rectangular plates along the length corresponding to the outer dimension of the diameter of the base 1 of the body (FIG. .4), and the front and rear walls 3, 4 - the height of the side walls 3 of the frame (figure 2). The width of the walls of the retainer (figure 5) is approximately 1/5 of their length.

 The frame (Fig. 2) is intended for the location of biopsy specimens in cells 1b. One hole 1a on the bottom wall 1 and two holes 2a on the upper wall 2 of the frame serve to fill the compact container with chemical reagents during the processing of morphological material.

 The bracket (Fig. 3) is intended for fixing biopsy samples in cells 1b of the frame (Fig. 2) and forming a block of preparations. The upper horizontal segments of the side walls 3 of the bracket (figure 3) are designed to remove the bracket with a block of drugs from the body (figure 4).

 The housing (figure 4) is a reservoir for reagents, serves as a matrix for the formation of a block of drugs, and also connects the frame (figure 2) and the bracket (figure 3) into a single design.

 The latch (Fig. 5) is designed to hold all the components of the morphological compact container (Fig. 1) in working condition, namely the frame (Fig. 2), the bracket (Fig. 3) and the body (Fig. 4).

 An example of a specific use of a morphological compact container.

Biopsy material is obtained endoscopically or surgically. Biopsy samples in the amount of 1-5 pieces are placed in order in the cells 1b of the bottom wall 1 of the frame (Fig. 2), covered with a bracket (Fig. 3), a body (Fig. 4) and fastened with a latch (Fig. 5). The compact container (Fig. 1) is turned 180 ^° around its horizontal axis and placed in a vessel with a formalin solution. On the registration form for sending biopsy material to the morphological laboratory, there is a cliché of the biopsy organ, where the arrows indicate the place from which this biopsy was obtained. Each arrow is sequentially numbered and each number will correspond to the serial number of the cell 1b of the frame (figure 2), in which this biopsy was placed. In a paraffin block, all biopsy specimens retain their original spatial orientation with respect to “their” cells. The number of this biopsy specimen will correspond to the number of the cell in which it was located, and the place from which it was obtained is shown in the registration form. This technique allows you to clearly label each biopsy.

 The resulting paraffin polybioptate block with a given location of biopsy specimens microtome. At the same time, the entire series of biopsy samples is simultaneously cut, which makes it possible to obtain histological preparations with the presence of the simultaneously targeted series of slices on one slide. This achieves significant savings in glass slides and subsequently in the dyeing of dye preparations.

 The part of the block with morphological material remaining after microtomation is placed in the cavity of the frame (Fig. 2), the frame is inserted into the bracket (Fig. 3) and the body (Fig. 4), fastened with a latch (Fig. 5). The block with morphological material can be stored in a compact container for a long time and, if necessary, it can be reused.

Efficiency of using a morphological compact container:
- the location of the holes on the lower and upper walls of the frame provides sufficient contact of the morphological material inside the compact container with chemical reagents at all stages of processing:
- morphological compact container allows you to standardize the processing of morphological material and makes the methodology efficient, economical, simple;
- the device of the compact container allows for clear labeling of biopsy specimens;
- design features of the morphological compact container make it possible to obtain a multibioptate block that can be easily microtomized;
- the obtained multibioptate histological preparations prepared by this method have increased information content, which in general significantly improves the diagnosis and contributes to the effective treatment of patients.

Claims (1)

 MORPHOLOGICAL COMPACT CONTAINER containing a frame with cells for biopsy specimens, a bracket for their fixation, a housing serving as a reservoir for reagents and a matrix for the preparation unit, and a latch for holding all structural elements in working condition, characterized in that one is made in the bottom wall of the frame a hole corresponding to the size and shape of the cells, and two holes are made in the upper wall of the frame for free penetration of chemicals in the processing of morphological material.

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