RU218151U1 - Chamber for histological studies in a humid environment - Google Patents

Abstract

The utility model relates to laboratory equipment, in particular to equipment for the incubation of biological samples in a humid environment. The chamber consists of a body, in the inner space of which there are cells with cylinders at the bottom for placing slides. On the narrow sides of the cells there are slots for gripping the glass slide with fingers or tweezers. At the level of the bottom, the cells are interconnected by holes. In the upper part of the chamber body, there are grooves for inserting the wet chamber cover. The lid covering the wet chamber has a slit on its top side for finger grip and movement. The utility model is needed to expand the arsenal of laboratory technical tools used to reduce the evaporation of liquid reagents from the surface of slides during the incubation of biological samples, which allows saving liquid reagents, avoiding drying and damage to samples.

Description

The utility model relates to laboratory equipment, in particular to equipment for the incubation of biological samples in a humid environment.

The closest utility model is the slide chamber, a multifunctional staining chamber (https://www.pathnsitu.com/productDetails.php?id=1175), which consists of a base with two built-in slide holders and a lid. The camera is made of opaque plastic material. The disadvantages of this technical solution are:

1) the inability to hold the base of the camera with your hand when opening the lid, which can lead to the movement of the camera following the lid and the movement of the reagents applied to the slides, as a result of which the reagents can be displaced from the surface of the slide;

2) chamber dimensions (39.7(length) × 25.5(width) × 4(height) cm) do not allow its use in small thermostats or incubator shakers;

3) fixation of glasses is carried out by two pairs of protrusions-clamps only on two sides of the glass slide. When the camera is tilted, longitudinal movement of slides on the bracket is possible, which can also affect the quality of the experiment.

The task to be solved by the claimed utility model is the creation of a full-fledged, specialized compact laboratory wet chamber.

This problem is solved using the claimed camera for histological studies in a humid environment, includes a body, in the internal space of which there are cells that serve as a place for placing slides and restrict their movement, also on the narrow sides of the cells there are slots for gripping the slide with fingers or tweezers, on at the bottom of the cells there are cylinders that serve as a support for the placed glass slide and hold it above the bottom of the cell, also at the level of the bottom of the cell are interconnected by holes, in the upper part of the chamber body there are grooves for inserting and moving the cover of the wet chamber, which has a slot on its upper side to grab it with your fingers and move it.

The distinguishing features of the proposed device are that

chamber for histological studies in a humid environment has a retractable roof, equipped with a slot for gripping with fingers and further movement;

the chamber for histological studies in a humid environment is small in size, allowing it to be used even in small thermostats and incubator shakers;

the cells securely fix the slides, excluding their movement when the wet chamber is tilted;

the dimensions of the chamber ready for use are 160×105×28 mm.

The essence of the utility model is illustrated by drawings (Fig. 1-4).

The principle of operation of the chamber for histological studies in a humid environment: body 1 is filled with water to level 2, a glass slide with the test sample is placed in cell 3 on cylinders 4, a cover 6 with a slot 7 is inserted from above into the grooves 5 of the body of the wet chamber 1.

Thus, the proposed utility model can be used as a full-fledged, specialized laboratory chamber for histological studies in a humid environment, used to reduce the evaporation of liquid reagents from the surface of slides during histological studies.

Device Description

Example 1. The device works as follows.

Cover 6 must be removed from body 1. Distilled water is poured into body 1 to level 2. In cell 3, on cylinders 4, a glass slide with the test sample and liquid reagent applied to it is placed. Then, cover 6 is inserted into the grooves 5 of the body of the chamber for histological studies in a humid environment 1, by hooking fingers into the slot 7, the cover 6 completely closes the body 1. Due to the tight fit of the surfaces of the cover 6 and the body of the camera for histological studies in a humid environment 1, as well as the volume of distilled water in the body of the chamber for histological studies in a humid environment 1, a zone with saturated water vapor is created in the internal volume of the chamber for histological studies in a humid environment, which does not allow liquid reagents to evaporate from the surface of the slides until the end of the experiment. Thanks to the device, it is possible to use liquid reagents on the surface of glass slides with biological samples for histological, molecular genetic, cytogenetic experiments.

Claims (1)

A chamber for histological studies in a humid environment, containing a chamber body, a cover, cells for placing glasses and limiting their movement, characterized in that it is made with dimensions of 160 × 105 × 28 mm, slots are located on the narrow sides of the cells, cylinders are located at the bottom of the cells, serving as a support for the placed slide and holding it above the bottom of the cell, at the level of the bottom of the cell are interconnected by holes, and in the upper part of the body there are slots for inserting and moving the cover, made with a longitudinal slot on its upper side.

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