CN113432957A

CN113432957A - Embedding box

Info

Publication number: CN113432957A
Application number: CN202110720779.4A
Authority: CN
Inventors: 王亮亮
Original assignee: First Affiliated Hospital of Guangzhou University of Chinese Medicine
Current assignee: First Affiliated Hospital of Guangzhou University of Chinese Medicine
Priority date: 2021-06-28
Filing date: 2021-06-28
Publication date: 2021-09-24

Abstract

The invention relates to the technical field of storage boxes, and provides an embedding box, which comprises: the storage box comprises a base with a first concave cavity, a cover body covering the first concave cavity, and a storage box with a second concave cavity; the storage box is arranged in the first concave cavity, and the opening of the second concave cavity faces the cover body; the second concave cavities are separated by partition plates to form a plurality of third concave cavities; the opening of each third cavity faces the cover body, and through holes communicated with the plurality of third cavities are formed in the cover body. Outside wax can enter into the third cavity through the through-hole after the liquefaction and carry out the wax dipping treatment to the tissue in the third cavity, and follow-up when slicing into slices to the wax stone, can slice the polylith tissue simultaneously, and is very convenient.

Description

Embedding box

Technical Field

The invention belongs to the technical field of storage boxes, and particularly relates to an embedding box.

Background

During modern experimental or medical testing, it is often necessary to section tissue (tissue: e.g., an organ or individual micro-organisms), and prior to sectioning, it is often necessary to enclose the tissue in a mesh-containing cassette (e.g., an embedding cassette). Generally, each cassette is placed inside one tissue block, and if there are many tissue blocks to be tested, many cassettes are needed. If a plurality of tissues need to be detected, each tissue needs to be sliced and detected separately, which is very troublesome.

Disclosure of Invention

The invention aims to provide an embedding box, which aims to solve the technical problem that if a plurality of tissues need to be detected, each tissue needs to be sliced and detected separately, which is very troublesome in the prior art.

In order to achieve the purpose, the invention adopts the technical scheme that: there is provided an embedding cassette comprising: the storage box comprises a base with a first concave cavity, a cover body covering the first concave cavity, and a storage box with a second concave cavity; the storage box is arranged in the first concave cavity, and the opening of the second concave cavity faces the cover body; the second concave cavities are separated by partition plates to form a plurality of third concave cavities; the opening of each third cavity faces the cover body, and through holes communicated with the plurality of third cavities are formed in the cover body.

Further, each of the third cavities is in a rectangular parallelepiped shape.

Further, a plurality of the third cavities are arranged in a matrix.

Further, the outer surface of the storage box is attached to the inner wall of the first concave cavity.

Further, the second cavity is rectangular.

Furthermore, at least one through hole is formed in the position, facing the cover body, of the opening of each third cavity.

Further, the thickness of the separator is 0.8mm to 1.2 mm.

Further, the thickness of the separator is 1 mm.

Further, the depth of the third cavity is 7mm to 10 mm.

Further, the depth of the third cavity is 9 mm.

The embedding box provided by the invention has the beneficial effects that: compared with the prior art, when tissues need to be detected, the embedding box provided by the invention can be used for respectively placing a plurality of tissues into different third concave cavities in the containing box, then placing the containing box into the first concave cavity on the base, and finally covering the cover body; external wax can be liquefied and then enters the third cavity through the through hole, the tissues in the third cavity are subjected to wax dipping treatment, and when wax blocks are subsequently sliced, a plurality of tissues can be sliced at the same time, so that the method is very convenient; and a plurality of tissues after slicing can be simultaneously treated or observed by other medicaments, so that the method is very convenient.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.

Fig. 1 is a schematic perspective view of a storage box according to an embodiment of the present invention;

fig. 2 is a schematic top view of a storage box according to an embodiment of the present invention;

FIG. 3 is a schematic perspective view of a storage case of the present invention being placed in a first cavity of a base;

fig. 4 is a schematic top view of a storage box according to an embodiment of the present invention, the storage box being placed in a first cavity of a base.

Wherein, in the figures, the respective reference numerals:

1-a base; 11-a first cavity; 2-a storage box; 21-a second cavity; 211-a third cavity; 212-liquid passing hole; 22-partition plate.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.

It should be noted that in the description of the embodiments of the present application, "/" indicates an OR meaning unless otherwise stated, for example, A/B may indicate A or B; "and/or" herein is merely an association describing an associated object, and means that there may be three relationships, e.g., a and/or B, which may mean: a exists alone, A and B exist simultaneously, and B exists alone. Wherein, A and B can be singular or plural respectively.

It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings, which is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and is therefore not to be construed as limiting the invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

Referring to fig. 1 to 4, the cassette according to the present invention will now be described. An embedding cassette comprising: the base 1 with a first cavity 11, the cover body covering the first cavity 11, and the storage box 2 with a second cavity 21; the storage box 2 is arranged in the first concave cavity 11, and the opening of the second concave cavity 21 faces the cover body; the second cavity 21 is partitioned by the partition plate 22 to form a plurality of third cavities 211; the opening of each third cavity 211 faces the cover body, and through holes respectively communicated with the plurality of third cavities 211 are formed in the cover body.

Thus, when the tissues need to be detected, a plurality of tissues can be respectively placed into different third cavities 211 in the storage box 2, then the storage box 2 is placed into the first cavity 11 on the base 1, and finally the cover body is covered; external wax (or other reagents) can be liquefied and then enter the third cavity 211 through the through holes, the tissues in the third cavity 211 are subjected to wax dipping treatment, and when wax blocks (a plurality of tissues are bonded through the wax blocks) are subsequently sliced, a plurality of tissues can be sliced at the same time, so that the slicing is very convenient; in addition, a plurality of tissues after being sliced can be simultaneously treated or observed by other medicaments, so that the method is very convenient.

Wherein, embedding box: a cassette for placing and storing tissue.

In one embodiment, the bottom surface of the base 1 is flat for resting on a horizontal table top.

In one embodiment, the cover is placed on the top surface of the base 1.

In one embodiment, the top surface of the base 1 is recessed downward to form a first cavity 11.

In one embodiment, the top surface of the housing case 2 is recessed downward to form a second cavity 21.

In one embodiment, the top surface of the housing case 2 is recessed downward to form a third cavity 211.

In one embodiment, the partition 22 extends in a vertical direction.

In one embodiment, the manifold 2 and the baffle 22 are each made of a flexible material that can be cut along with the wax block.

In one embodiment, the storage case 2 and the partition 22 are made of the same material, respectively.

In one embodiment, the bottom wall of the third cavity 211 is opened with a liquid through hole 212. In this manner, it is facilitated that the liquid medicament or wax can permeate into the third cavity 211 through the liquid passing hole 212.

In one embodiment, tissue (in one embodiment, tissue: small pieces of tissue for immunohistochemical or pathological staining techniques) placed in third cavity 211 may be dehydrated with alcohol (e.g., graded alcohol), cleared with xylene, and embedded with wax. In one embodiment, following wax immersion embedding, subsequent tissue staining; the method comprises the following steps: the tissue specimen is sliced up, the slices are mounted with slides, dehydrated with alcohol (e.g., gradient alcohol), and the slide-up specimen is stained with an antibody or stain (in one embodiment, the incubation time of each slice is kept highly consistent to ensure the uniformity of the staining conditions). Thus, it is very convenient to detect or observe the tissue.

In one embodiment, separate arrays of cells (i.e., third cavity 211) in the cassette (e.g., a 5 x 5 unified cell array; optionally, the cells having a single-sided backing web) may each contain a predetermined individual tissue specimen, i.e., tens of tissue specimens may be dehydrated, transparentized, and waxed simultaneously in the cassette; a block of wax contains a batch of tissue specimens arranged in an array. In one embodiment, the individual cells of the array in the cassette (i.e., the third cavity 211) are made of a material with special characteristics (e.g., PLA material or ABS plastic, which has the characteristics of melting point > 100 ℃, being insoluble in xylene or alcohol, having low hardness and being easily sliced by a paraffin slicer).

In one embodiment, the waxed independent array containing a plurality of specimens is taken out from the storage box 2, and is integrally manufactured into a paraffin embedding block, then paraffin slicing is carried out, at this time, one slice already contains dozens of tissue specimens, the slice is attached to a glass slide, subsequent dehydration and incubation with alcohol (such as gradient alcohol) or staining agent are carried out, dozens of tissue specimens on the same glass slide are completely consistent in the processing duration of each reagent, so that the high homogenization of various processing parameters of a batch of specimens is achieved, the background staining of different specimens is completely consistent, the positive staining of different specimens is clear at this time, the photographing of a plurality of specimens on one glass slide is also carried out by the microscope photographing, or the scanning technology of the whole glass slide with ultra-high definition (such as 4K images) is adopted, there is no error of inconsistent photographing light intensity caused by repeatedly adjusting the exposure. In conclusion: in one embodiment, (1) by designing the array mesh grid in one embedding frame, a plurality of tissue samples of different individuals are placed in one embedding frame, the samples are embedded in the same paraffin block, the subsequent slicing, antibody or stain adding incubation time and dosage are completely synchronized, and the operation time error and the reagent adding dosage error among different samples do not exist, so that the analysis of a staining result is more reliable; (2) the same slide contains a plurality of individual tissues, and the scanning and photographing technology of the ultra-high-definition whole slide can avoid artificially selecting a specimen with a desired staining result (reduce the possibility of picture counterfeiting); (3) the single slice that contains a plurality of tissue samples, subsequent dyeing process compares with traditional single sample single slice, will show and save antibody and staining reagent, is showing the number of times that reduces repetitive operation simultaneously, improves detection efficiency.

In one embodiment, the embedding box is made of a polymer material Polylactic acid (PLA) through printing by a 3D printer. In one embodiment, the melting point of the PLA material is 173-178 ℃, the Rockwell hardness is 70-90, the heat-resistant temperature is 110 ℃, the PLA material is insoluble in water and insoluble in organic solvents such as ethanol, xylene and paraffin.

In one embodiment, the plurality of third cavities 211 employs a 5 x 4 array grid of 20 cells. In one embodiment, the size of the cell is 6mm x 6mm, the thickness of the cell side wall is 1mm, and the depth of the cell is 9mm), 20 different samples can be placed.

In one embodiment, methods are used, for example: in the same batch of animal experiments, 4 different drug treatment groups are divided into 5 rats (20 rats in total) in each group, after the rats are killed, cutting 1 small tissue block from liver tissue of each rat, placing into small cell (i.e. third cavity 211) one by one according to the marked sequence, covering with cover body, placing into alcohol (such as gradient alcohol) for dehydration, making xylene transparent, soaking in wax, the cover is opened on the operating table at 62 ℃, the grid (i.e. the storage box 2 with the plurality of third cavities 211) is taken out, the grid as a whole is subjected to paraffin embedding, then paraffin sectioning is carried out, the grid is attached to a glass slide (the glass slide comprises liver tissues of 20 different animals) to be subjected to subsequent pathological staining or immunohistochemical staining, then microscopic photographing is carried out (or a high-resolution scanner is used for scanning and acquiring images of the whole glass slide), and the staining analysis software is used for carrying out staining analysis.

In one embodiment, the top surface of the base 1 has a slope that connects with the edge of the first cavity 11. So, be convenient for the user finger through the slope press base 1, and be difficult to can touch the finger when opening and shutting the lid.

In one embodiment, one end of the cover is pivotally connected to the base 1 through a pivot, and the other end of the cover is fastened to the base 1 through a fastening structure. Thus, the cover body is very convenient to open and close. In one embodiment, the fastening structure includes a hook disposed on the cover and a recess disposed on the base 1, and the hook is detachably hooked in the recess. In one embodiment, the depression is located at the edge of the first cavity 11; the partition 22 meets the edge of the second cavity 21 to form a meeting portion, and the meeting portion is adjacent to the edge of the recess. In one embodiment, the predetermined edge of the depression that is critical to the intersection extends in a straight line, and the partition 22 is disposed perpendicular to the direction of extension of the predetermined edge.

In one embodiment, the number of the partition boards 22 is multiple, a part of the partition boards 22 are respectively arranged vertically in the transverse direction, a part of the partition boards 22 are respectively arranged vertically in the longitudinal direction, and each partition board 22 arranged vertically in the transverse direction meets each partition board 22 arranged vertically in the longitudinal direction.

In one embodiment, each third cavity 211 is provided with a matrix of liquid passing holes 212 therein. In one embodiment, the number of through-liquid holes 212 in each third cavity 211 is 4 x 4 (i.e., 16) in the array.

In one embodiment, the bottom surfaces of the third cavities 211 are located in the same plane. In this way, the height of the object in third cavity 211 is kept uniform.

In one embodiment, the bottom surface of third cavity 211 is perpendicular to the direction in which third cavity 211 is oriented. In this manner, the object placed in third cavity 211 can be uniformly supported on the bottom surface of third cavity 211.

Further, referring to fig. 1 to 4, as an embodiment of the embedding box provided by the present invention, each of the third cavities 211 has a rectangular parallelepiped shape. Thus, the formation is very convenient.

Further, referring to fig. 1 to 4, as an embodiment of the embedding cassette provided by the present invention, a plurality of third cavities 211 are arranged in a matrix. This facilitates the placement of tissue into each of the third plurality of cavities 211.

Further, referring to fig. 1 to 4, as an embodiment of the embedding box provided by the present invention, an outer surface of the storage box 2 is attached to an inner wall of the first cavity 11. Thus, the contact between the housing case 2 and the inner wall of the first cavity 11 is made tighter.

Further, referring to fig. 1 to 4, as an embodiment of the embedding box provided by the present invention, the second cavity 21 is rectangular. In this way, it is very convenient to form the second cavity 21.

Further, referring to fig. 1 to 4, as an embodiment of the embedding box provided by the present invention, at least one through hole is disposed at a position where an opening of each third cavity 211 faces the cover body. In this way, the wax or other agent in liquid form can be easily introduced into the different third cavities 211 through the different through holes.

Further, referring to fig. 1 to 4, as an embodiment of the embedding cassette provided by the present invention, the thickness of the partition plate 22 is 0.8mm to 1.2 mm. Therefore, the processing is convenient, and the volume is reduced.

Further, referring to fig. 1 to 4, as an embodiment of the embedding cassette provided by the present invention, the thickness of the partition plate 22 is 1 mm. Therefore, the processing is convenient, and the volume is reduced.

Further, referring to fig. 1 to 4, as an embodiment of the embedding cassette of the present invention, the depth of the third cavity is 7mm to 10 mm. Thus, the tissue is convenient to put in.

Further, referring to fig. 1 to 4, as an embodiment of the embedding cassette provided by the present invention, the depth of the third cavity is 9 mm. Thus, the tissue is convenient to put in.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims

1. An embedding cassette, comprising: the storage box comprises a base with a first concave cavity, a cover body covering the first concave cavity, and a storage box with a second concave cavity; the storage box is arranged in the first concave cavity, and the opening of the second concave cavity faces the cover body; the second concave cavities are separated by partition plates to form a plurality of third concave cavities; the opening of each third cavity faces the cover body, and through holes communicated with the plurality of third cavities are formed in the cover body.

2. The cassette of claim 1, wherein each of the third cavities is rectangular.

3. The cassette of claim 1, wherein a plurality of the third cavities are arranged in a matrix.

4. The cassette of claim 1, wherein an outer surface of the storage cassette is positioned against an inner wall of the first cavity.

5. The cassette of claim 4, wherein the second cavity is rectangular.

6. The embedding cassette of claim 1, wherein the opening of each third cavity is provided with at least one through hole at a position facing the cover.

7. The embedding cassette of any one of claims 1 to 6, wherein the spacer has a thickness of 0.8mm to 1.2 mm.

8. The embedding cassette of claim 7, wherein the spacer has a thickness of 1 mm.

9. The cassette of any one of claims 1 to 6, wherein the third cavity has a depth of 7mm to 10 mm.

10. The cassette of claim 9, wherein the third cavity has a depth of 9 mm.