RU2019114407A - Криозащитный агент, содержащий экзополисахарид из pseudoalteromonas sp. сy01 - Google Patents

Криозащитный агент, содержащий экзополисахарид из pseudoalteromonas sp. сy01 Download PDF

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RU2019114407A
RU2019114407A RU2019114407A RU2019114407A RU2019114407A RU 2019114407 A RU2019114407 A RU 2019114407A RU 2019114407 A RU2019114407 A RU 2019114407A RU 2019114407 A RU2019114407 A RU 2019114407A RU 2019114407 A RU2019114407 A RU 2019114407A
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Prior art keywords
exopolysaccharide
pseudoalteromonas
agent containing
kctc
cryoprotective agent
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RU2019114407A
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English (en)
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RU2779448C2 (ru
RU2019114407A3 (ru
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Ён Ан ИМ
Ил-Чхан КИМ
Се Чон АН
Уй Ён ЮН
Хон Гым ЛИ
Сун Чин КИМ
Бил-Сун КАН
Чун Ын КИМ
Тхэ Гён КИМ
Ха Чу ПАК
Чин Хён СОН
Мин Чу КИМ
Чу Ми ХОН
Дон-Кё ЧО
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Альтеробиотек Инк.
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Polymers & Plastics (AREA)
  • Materials Engineering (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Claims (11)

1. Экзополисахарид, который продуцирован штаммом Pseudoalteromonas sp. CY01 (KCTC 12867ВР) и состоит из глюкозы и галактозы.
2. Экзополисахарид по п. 1, отличающийся тем, что молярное соотношение глюкозы и галактозы составляет приблизительно 3,4:1.
3. Экзополисахарид, имеющий молекулярную массу от 1,0×105 до 4,3×105 Да, который получен посредством гидролиза экзополисахарида по п. 1.
4. Композиция для криозащиты клеток, которая содержит экзополисахарид по п. 1 или 3.
5. Композиция по п. 4, дополнительно содержащая глицерин или ДМСО.
6. Способ получения экзополисахарида по п. 1, включающий следующие стадии:
(a) культивирование штамма Pseudoalteromonas sp. CY01 (KCTC 12867ВР) с получением указанного экзополисахарида; и
(b) выделение полученного экзополисахарида.
7. Способ получения экзополисахарида, имеющего молекулярную массу от 1,0×105 до 4,3×105 Да по п. 3, включающий стадию гидролиза экзополисахарида по п. 1.
8. Способ криоконсервации клеток с применением экзополисахарида по п. 1 или 3.
9. Штамм Pseudoalteromonas sp. CY01 (KCTC 12867ВР), который обладает способностью продуцировать экзополисахарид слизи.
RU2019114407A 2017-05-11 2017-12-18 Криозащитный агент, содержащий экзополисахарид из pseudoalteromonas sp. сy01 RU2779448C2 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR10-2017-0058701 2017-05-11
KR1020170058701A KR101816802B1 (ko) 2017-05-11 2017-05-11 Pseudoalteromonas sp. Strain CY01 균주 유래 세포외다당을 함유하는 동결보호제
US15/836,138 US10993434B2 (en) 2017-05-11 2017-12-08 Cryoprotective agent containing exopolysaccharide from Pseudoalteromonas sp. CY01
US15/836,138 2017-12-08
PCT/KR2017/014909 WO2018207988A1 (en) 2017-05-11 2017-12-18 Cryoprotective agent containing exopolysaccharide from pseudoalteromonas sp. cy01

Publications (3)

Publication Number Publication Date
RU2019114407A true RU2019114407A (ru) 2020-11-13
RU2019114407A3 RU2019114407A3 (ru) 2021-02-26
RU2779448C2 RU2779448C2 (ru) 2022-09-08

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Also Published As

Publication number Publication date
EP3401389A1 (en) 2018-11-14
CA3043814A1 (en) 2018-11-15
KR101816802B1 (ko) 2018-01-11
JP2019530471A (ja) 2019-10-24
WO2018207988A1 (en) 2018-11-15
CN108864315B (zh) 2020-09-11
AU2017413156A1 (en) 2019-06-06
CA3043814C (en) 2022-02-15
RU2019114407A3 (ru) 2021-02-26
US10993434B2 (en) 2021-05-04
US20180325101A1 (en) 2018-11-15
JP6768959B2 (ja) 2020-10-14
CN108864315A (zh) 2018-11-23
AU2017413156B2 (en) 2021-09-09

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