RU2018145047A - Обнаружение антигенов трихинеллы - Google Patents
Обнаружение антигенов трихинеллы Download PDFInfo
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- RU2018145047A RU2018145047A RU2018145047A RU2018145047A RU2018145047A RU 2018145047 A RU2018145047 A RU 2018145047A RU 2018145047 A RU2018145047 A RU 2018145047A RU 2018145047 A RU2018145047 A RU 2018145047A RU 2018145047 A RU2018145047 A RU 2018145047A
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- RU
- Russia
- Prior art keywords
- trichinella
- antigens
- tissue
- samples
- antibody
- Prior art date
Links
- 239000000427 antigen Substances 0.000 title claims 12
- 102000036639 antigens Human genes 0.000 title claims 12
- 108091007433 antigens Proteins 0.000 title claims 12
- 241000243774 Trichinella Species 0.000 title claims 10
- 238000001514 detection method Methods 0.000 title claims 7
- 238000000034 method Methods 0.000 claims 11
- 210000001519 tissue Anatomy 0.000 claims 10
- 239000000284 extract Substances 0.000 claims 7
- 238000004458 analytical method Methods 0.000 claims 4
- 230000002860 competitive effect Effects 0.000 claims 2
- 238000006073 displacement reaction Methods 0.000 claims 2
- 238000007689 inspection Methods 0.000 claims 2
- 235000013372 meat Nutrition 0.000 claims 2
- 239000002245 particle Substances 0.000 claims 2
- 238000002965 ELISA Methods 0.000 claims 1
- 241000243777 Trichinella spiralis Species 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000008187 granular material Substances 0.000 claims 1
- 238000003018 immunoassay Methods 0.000 claims 1
- 210000003205 muscle Anatomy 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
- 230000011664 signaling Effects 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 229940096911 trichinella spiralis Drugs 0.000 claims 1
- 238000001262 western blot Methods 0.000 claims 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43536—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms
- C07K14/4354—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms from nematodes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/12—Meat; Fish
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48735—Investigating suspensions of cells, e.g. measuring microbe concentration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
- G01N2333/43526—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from worms
- G01N2333/4353—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from worms from nematodes
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Virology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Claims (22)
1. Способ обнаружения Trichinella в образцах экстрактов тканей, при этом в образцах экстрактов тканей 90% частиц имеет диаметр 300 мкм или менее (D90≤300 мкм).
2. Способ по п. 1, при этом образцы экстрактов тканей представляют собой образцы тканей млекопитающего, предпочтительно образцы тканей свиньи.
3. Способ по п. 1 или 2, при этом в образцах экстрактов тканей 90% частиц имеют диаметр 250 мкм или менее (D90≤250 мкм), предпочтительно 200 мкм или менее (D90≤200 мкм).
4. Способ по пп. 1-3, при этом образцы экстрактов тканей происходят из мышечных тканей.
5. Способ по пп. 1-4, при этом при получении образцов экстрактов тканей
(a) температура не превышает 45°C, предпочтительно 40°C; и/или
(b) не происходит никакого ферментативного и/или химического расщепления тканей.
6. Способ по пп. 1-5, при этом способ
(a) не включает стадий микроскопии;
(b) применяется в рамках санитарного осмотра мяса; и/или
(c) имеет предел обнаружения≤7 нг антигена на мл экстракта тканей.
7. Способ по пп. 1-6, при этом способ проводят с помощью иммунного анализа, предпочтительно с помощью анализа ELISA, анализа в боковом потоке, лайн-блоттинга, вестерн-блоттинга, анализа гранул, анализа на вертикальном фильтре или 3D-иммунофильтрационного анализа.
8. Способ по пп. 1-7, при этом Trichinella представляет собой Trichinella spiralis.
9. Применение системы обнаружения для обнаружения Trichinella, предпочтительно в санитарном осмотре мяса, при этом система обнаружения включает
(a) носитель обнаружения, первое антитело к одному и/или нескольким антигенам Trichinella, и
(b)(i) второе антитело к одному и/или нескольким антигенам Trichinella, при этом второе антитело связано с сигнальной молекулой, или
(b)(ii) включает один или несколько антигенов Trichinella, которые связаны с сигнальной молекулой, при этом антигены из (b)(ii) образованы таким образом, что они вызывают связывание антигенов (а) с первыми антителами путем конкурентного вытеснения.
10. Комплект, включающий
(a) носитель обнаружения, который содержит первое антитело к одному и/или нескольким антигенам Trichinella, и
(b)(i) второе антитело к одному и/или нескольким антигенам Trichinella, при этом второе антитело связано с сигнальной молекулой, или
(b)(ii) один или несколько антигенов Trichinella, которые связаны с сигнальными молекулами, при этом антигены из (b)(ii) образованы таким образом, что они вызывают связывание антигенов (а) с первыми антителами путем конкурентного вытеснения.
11. Комплект по п. 10, при этом данный комплект включает описание способа по пп. 1-8.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17208994.8A EP3502685A1 (de) | 2017-12-20 | 2017-12-20 | Antigennachweis von trichinella |
| EP17208994.8 | 2017-12-20 | ||
| EP18199404 | 2018-10-09 | ||
| EP18199404.7 | 2018-10-09 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2018145047A true RU2018145047A (ru) | 2020-06-19 |
| RU2018145047A3 RU2018145047A3 (ru) | 2020-12-11 |
| RU2746448C2 RU2746448C2 (ru) | 2021-04-14 |
Family
ID=64453433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2018145047A RU2746448C2 (ru) | 2017-12-20 | 2018-12-19 | Способ обнаружения Trichinella |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10663460B2 (ru) |
| EP (1) | EP3502686B1 (ru) |
| CN (1) | CN109946448B (ru) |
| BR (1) | BR102018076467A2 (ru) |
| CA (1) | CA3027972C (ru) |
| ES (1) | ES2966980T3 (ru) |
| PL (1) | PL3502686T3 (ru) |
| RU (1) | RU2746448C2 (ru) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI793451B (zh) * | 2019-09-20 | 2023-02-21 | 納維再生科技股份有限公司 | 個人化癌症免疫治療 |
| RU2768162C1 (ru) * | 2021-06-09 | 2022-03-23 | Мария Владимировна Видманова | Способ подготовки половозрелых особей нематод для идентификации методом maldi tof масс-спектрометрии |
| WO2023230624A2 (en) * | 2022-05-27 | 2023-11-30 | Taipei Medical University | Epha2-targeting antibodies and their applications in cancer treatment |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3314937A1 (de) * | 1983-04-25 | 1984-10-31 | Gottfried Prof. Dr. 8057 Eching Pfeiffer | Vorrichtung und verfahren zur erzeugung bzw. freisetzung und abtrennung von substanzen oder partikulaeren gebilden aus fluessiger, plastischer oder fester materie und verwendung der vorrichtung |
| DE4004537C1 (en) * | 1990-02-14 | 1991-02-28 | Haag, Heinz, 8533 Scheinfeld, De | Meat sample examination for parasites - by mixing meat e.g. pork with pepsin and dilute hydrochloric acid in container etc. |
| WO2006034716A1 (en) * | 2004-09-30 | 2006-04-06 | Paratest Aps | A method and a system for detection of trichinella larvae in meat samples |
| FR2897063B1 (fr) * | 2006-02-07 | 2011-04-01 | Agronomique Inst Nat Rech | Polypeptides reconnus par des anticorps anti-trichinella, et leurs applications. |
| RU2339038C2 (ru) * | 2006-08-15 | 2008-11-20 | Всероссийский научно-исследовательский институт гельминтологии им. К.И. Скрябина (ВИГИС) | Способ прижизненной диагностики трихинеллеза плотоядных и всеядных животных |
| DE102008030129A1 (de) * | 2008-06-27 | 2010-01-21 | Prionics Ag | Verfahren zur Bestimmung von Trichinella-Infektionen und diagnostische Zusammensetzung für solche Verfahren |
| CA2765893C (en) * | 2009-06-19 | 2017-08-01 | Philipp Bauer | New method for isolating trichinella or other parasites from organic tissue |
| CN102331501A (zh) * | 2011-06-21 | 2012-01-25 | 郑州大学 | IgY－McAb夹心ELISA检测旋毛虫循环抗原的方法 |
-
2018
- 2018-12-03 EP EP18209802.0A patent/EP3502686B1/de active Active
- 2018-12-03 ES ES18209802T patent/ES2966980T3/es active Active
- 2018-12-03 PL PL18209802.0T patent/PL3502686T3/pl unknown
- 2018-12-18 CA CA3027972A patent/CA3027972C/en active Active
- 2018-12-19 US US16/225,007 patent/US10663460B2/en active Active
- 2018-12-19 RU RU2018145047A patent/RU2746448C2/ru active
- 2018-12-19 BR BR102018076467-5A patent/BR102018076467A2/pt active Search and Examination
- 2018-12-20 CN CN201811559017.5A patent/CN109946448B/zh active Active
-
2020
- 2020-04-07 US US16/841,728 patent/US10782286B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN109946448A (zh) | 2019-06-28 |
| US20190187132A1 (en) | 2019-06-20 |
| CA3027972C (en) | 2023-08-29 |
| EP3502686A1 (de) | 2019-06-26 |
| RU2018145047A3 (ru) | 2020-12-11 |
| EP3502686B1 (de) | 2023-11-22 |
| CN109946448B (zh) | 2023-10-03 |
| EP3502686C0 (de) | 2023-11-22 |
| US10663460B2 (en) | 2020-05-26 |
| ES2966980T3 (es) | 2024-04-25 |
| US20200232976A1 (en) | 2020-07-23 |
| RU2746448C2 (ru) | 2021-04-14 |
| BR102018076467A2 (pt) | 2020-06-23 |
| US10782286B2 (en) | 2020-09-22 |
| PL3502686T3 (pl) | 2024-04-15 |
| CA3027972A1 (en) | 2019-06-20 |
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