RU2018136932A - Микрожидкостный чип для фокусировки потока содержащей частички жидкости - Google Patents

Микрожидкостный чип для фокусировки потока содержащей частички жидкости Download PDF

Info

Publication number
RU2018136932A
RU2018136932A RU2018136932A RU2018136932A RU2018136932A RU 2018136932 A RU2018136932 A RU 2018136932A RU 2018136932 A RU2018136932 A RU 2018136932A RU 2018136932 A RU2018136932 A RU 2018136932A RU 2018136932 A RU2018136932 A RU 2018136932A
Authority
RU
Russia
Prior art keywords
microfluidic
microfluidic channel
channel
particles
sample
Prior art date
Application number
RU2018136932A
Other languages
English (en)
Russian (ru)
Other versions
RU2018136932A3 (enExample
Inventor
Дмитрий КАШАНИН
Игорь ШВЕЦ
Франческо ДИКОРАТО
Original Assignee
Селликс Лимитед
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Селликс Лимитед filed Critical Селликс Лимитед
Publication of RU2018136932A publication Critical patent/RU2018136932A/ru
Publication of RU2018136932A3 publication Critical patent/RU2018136932A3/ru

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0463—Hydrodynamic forces, venturi nozzles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0469—Buoyancy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0042—Investigating dispersion of solids
    • G01N2015/0053—Investigating dispersion of solids in liquids, e.g. trouble
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N2015/1413—Hydrodynamic focussing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Fluid Mechanics (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Micromachines (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
RU2018136932A 2016-04-20 2017-04-20 Микрожидкостный чип для фокусировки потока содержащей частички жидкости RU2018136932A (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16166177.2 2016-04-20
EP16166177 2016-04-20
PCT/EP2017/059453 WO2017182599A1 (en) 2016-04-20 2017-04-20 A microfluidic chip for focussing a stream of particulate containing fluid

Publications (2)

Publication Number Publication Date
RU2018136932A true RU2018136932A (ru) 2020-05-20
RU2018136932A3 RU2018136932A3 (enExample) 2020-09-07

Family

ID=55794906

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2018136932A RU2018136932A (ru) 2016-04-20 2017-04-20 Микрожидкостный чип для фокусировки потока содержащей частички жидкости

Country Status (10)

Country Link
US (1) US11633737B2 (enExample)
EP (1) EP3445492B1 (enExample)
JP (1) JP2019518971A (enExample)
CN (1) CN109689212A (enExample)
AU (1) AU2017251990A1 (enExample)
BR (1) BR112018071562A2 (enExample)
CA (1) CA3021609A1 (enExample)
MX (1) MX2018012862A (enExample)
RU (1) RU2018136932A (enExample)
WO (1) WO2017182599A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3556845A1 (en) 2018-04-20 2019-10-23 Cellix Limited A method and device for transfecting cells
EP3946733B1 (en) 2019-03-29 2024-05-08 Cellix Limited A method and device for transfecting cells
CA3097579A1 (en) 2019-10-28 2021-04-28 Op-Hygiene Ip Gmbh Method of identifying biologic particles
CN111135882B (zh) * 2019-12-30 2021-07-09 南方科技大学 一种二维流动聚焦装置
EP3871773B1 (en) 2020-02-27 2025-12-10 Cellix Limited A method of determining the transfection status of a plurality of cells
US20230408395A1 (en) * 2020-11-20 2023-12-21 The Trustees Of Princeton University System and method for pneumatic-free electronically driven microfluidics to allow massive scalability with integrated cellular and biomolecular detection
CN112698024B (zh) * 2020-12-08 2022-04-26 华中农业大学 一种基于差分阻抗颗粒计数的免疫分析方法
EP4308902A4 (en) * 2021-03-18 2025-01-22 Nanyang Technological University Method for label-free cell activation profiling using microfluidic impedance cytometry
CN114112826B (zh) * 2021-11-25 2024-04-19 天津大学 用于荧光微粒的声光互联微流控检测系统和检测方法
EP4465016A4 (en) * 2022-03-14 2025-11-26 Orange Biomed Co Ltd METHOD FOR MEASURING PARTICLES IN SOLUTION AND DEVICE FOR ITS IMPLEMENTATION
CN120082423B (zh) * 2025-05-06 2025-09-12 杭州谱康医学科技有限公司 微粒分选装置和方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5716852A (en) * 1996-03-29 1998-02-10 University Of Washington Microfabricated diffusion-based chemical sensor
US6437551B1 (en) * 1999-11-02 2002-08-20 The Regents Of The University Of California Microfabricated AC impedance sensor
JP4093740B2 (ja) 2001-09-27 2008-06-04 独立行政法人科学技術振興機構 微粒子分別マイクロチップと微粒子分別装置
US6703819B2 (en) * 2001-12-03 2004-03-09 Board Of Regents, The University Of Texas System Particle impedance sensor
GB2383127B (en) * 2001-12-12 2004-10-20 Proimmune Ltd Device and method for investigating analytes in liquid suspension or solution
AU2003216175A1 (en) * 2002-02-04 2003-09-02 Colorado School Of Mines Laminar flow-based separations of colloidal and cellular particles
DE102007017318B4 (de) 2007-04-11 2014-07-31 Bundesrepublik Deutschland, vertreten durch das Bundesministerium für Wirtschaft und Technologie, dieses vertreten durch den Präsidenten der Physikalisch-Technischen Bundesanstalt Verfahren zum hydrodynamischen Fokussieren eines Fluidstroms und Anordnung
JP4661942B2 (ja) 2008-05-13 2011-03-30 ソニー株式会社 マイクロチップとその流路構造
US8693762B2 (en) 2010-09-14 2014-04-08 The Regents Of The University Of California Inertial particle focusing flow cytometer
US9170138B2 (en) * 2010-10-01 2015-10-27 The Board Of Trustees Of The Leland Stanford Junior University Enhanced microfluidic electromagnetic measurements

Also Published As

Publication number Publication date
WO2017182599A1 (en) 2017-10-26
AU2017251990A1 (en) 2018-11-15
CA3021609A1 (en) 2017-10-26
US11633737B2 (en) 2023-04-25
US20190232290A1 (en) 2019-08-01
EP3445492A1 (en) 2019-02-27
RU2018136932A3 (enExample) 2020-09-07
BR112018071562A2 (pt) 2019-02-12
JP2019518971A (ja) 2019-07-04
MX2018012862A (es) 2019-07-04
EP3445492B1 (en) 2021-09-15
CN109689212A (zh) 2019-04-26

Similar Documents

Publication Publication Date Title
RU2018136932A (ru) Микрожидкостный чип для фокусировки потока содержащей частички жидкости
JP7354368B2 (ja) マイクロ流体チャネルを使用してマイクロ粒子のバルク選別を行う方法及び装置
Gong et al. New advances in microfluidic flow cytometry
EP3187854B1 (en) Particle image-capturing device and particle image-capturing method
JP4047336B2 (ja) ゲル電極付セルソーターチップ
JP6661198B2 (ja) 粒子分析装置
Chen et al. Microfluidic chip for plasma separation from undiluted human whole blood samples using low voltage contactless dielectrophoresis and capillary force
JP5691195B2 (ja) マイクロチップ及び微小粒子分析装置
US8941081B2 (en) Microparticle measurement apparatus and microparticle analysis method
US20050072677A1 (en) Dielectric particle focusing
US8736837B2 (en) Method and flow cell for characterizing particles by means of non-Gaussian temporal signals
Barat et al. Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer
US20210333194A1 (en) Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics
CN102087197A (zh) 全功能血液分析仪器中库尔特微孔的共轴照明方法及其分析仪器
JP2019518971A5 (enExample)
JP2013210287A (ja) 微小粒子分取装置におけるキャリブレーション方法、該装置及びキャリブレーション粒子
CN111133291B (zh) 用于落射荧光测量的光学流式细胞仪
JP4779261B2 (ja) 微粒子分離方法、微粒子分離装置、およびセンサ
Schiro et al. Continuous‐flow single‐molecule CE with high detection efficiency
JP2023027157A (ja) マイクロチップ及び微小粒子分取装置
KR20120041870A (ko) 다중 형광 정도 이용 세포 분리 장치 및 전극구조와 다중 형광 정도 이용 세포 분리 방법
JP5897681B2 (ja) マイクロチップ及び微小粒子分析装置
JP2016145834A (ja) 微小粒子分取装置及びキャリブレーション用粒子
Lundqvist et al. Electrophoretic separation and confocal laser‐induced fluorescence detection at ultralow concentrations in constricted fused‐silica capillaries
JP6782971B2 (ja) 磁場生成装置、測定セル、分析装置、及び粒子分離装置

Legal Events

Date Code Title Description
FA92 Acknowledgement of application withdrawn (lack of supplementary materials submitted)

Effective date: 20210701