RU2017144616A - Растение с повышенным поглощением кремния - Google Patents

Растение с повышенным поглощением кремния Download PDF

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RU2017144616A
RU2017144616A RU2017144616A RU2017144616A RU2017144616A RU 2017144616 A RU2017144616 A RU 2017144616A RU 2017144616 A RU2017144616 A RU 2017144616A RU 2017144616 A RU2017144616 A RU 2017144616A RU 2017144616 A RU2017144616 A RU 2017144616A
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nucleic acid
seq
marker
soybean plant
hikmok
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Ришар БЕЛАНЖЕ
Рупеш ДЕШМУКХ
Франсуа БЕЛЬЗИЛЬ
Каролина ЛАББЕ
Ажагувел ПЕРУМАЛ
С. Маттью ЭДВАРДС
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Юниверсите Лаваль
Зингента Партисипейшнс Аг
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Claims (17)

1. Способ идентификации или отбора первого растения сои, характеризующегося повышенным поглощением Si, при этом способ включает стадии:
a. выделения нуклеиновой кислоты из первого растения сои;
b. выявления наличия в нуклеиновой кислоте молекулярного маркера, связанного с повышенным поглощением Si, где молекулярный маркер связан с гаплотипом H1; или расположен в пределах 20 сМ, 10 сМ, 5 сМ, 1 сМ или 0,5 сМ от хромосомного интервала, соответствующего области генома из хромосомы 16 Hikmok sorip на расстоянии от приблизительно 92,6 сМ до приблизительно 132 сМ; или расположен в физических положениях от 33,15 млн пар оснований до 36,72 млн пар оснований, как указано на генетической карте сцепления Hikmok sorip (PI372415A); и
c. идентификации или отбора указанного растения сои на основании наличия молекулярного маркера из b);
с идентификацией или отбором таким образом первого растения сои, характеризующегося повышенным поглощением Si.
2. Способ по п. 1, где молекулярный маркер представляет собой однонуклеотидный полиморфизм (SNP), локус количественного признака (QTL), полиморфизм длин амплифицированных фрагментов (AFLP), случайно амплифицированную полиморфную ДНК (RAPD), полиморфизм длин рестрикционных фрагментов (RFLP) или микросателлит.
3. Способ по п. 1 или 2, где хромосомный интервал содержит любую нуклеиновую кислоту или ее часть, содержащие пары нуклеотидных оснований, соответствующие положениям 1-2658341 в SEQ ID NO: 1; 567613-569933 в SEQ ID NO: 1; 564321-567612 в SEQ ID NO: 1; 577172-579696 в SEQ ID NO: 1 или 573723-577171 в SEQ ID NO: 1.
4. Способ по любому из пп. 1-3, где идентифицированное или отобранное растение содержит по меньшей мере один маркер, соответствующий
а. области генома из хромосомы 16 Hikmok sorip на расстоянии от приблизительно 92,6 сМ до приблизительно 132 сМ; или области генома в физических положениях от 33,15 млн пар оснований до 36,72 млн пар оснований или ее части, как указано на генетической карте сцепления Hikmok sorip (PI372415A); или
b. SNP, выбранному из группы, состоящей из А(33673022), G(33673483), С(33681630), Т(33682500), G(33683047) и С(33683049) генов glyma30000 или glyma30020.
5. Способ по любому из пп. 1-4, где хромосомный интервал содержит нуклеиновую кислоту, кодирующую полипептид с аминокислотной последовательностью, содержащей SEQ ID NO: 15, и где дополнительно полипептид содержит по меньшей мере одну аминокислоту, соответствующую пролину в положении 5, изолейцину в положении 295 или валину в положении 439.
6. Способ по любому из пп. 1-5, где хромосомный интервал содержит нуклеиновую кислоту, кодирующую полипептид с аминокислотной последовательностью, содержащей SEQ ID NO: 17, где дополнительно полипептид содержит по меньшей мере одну аминокислоту, соответствующую гистидину в положении 322 или глицину в положении 431.
7. Способ по любому из пп. 1-6, где способ применяют в коммерческой селекционной программе по растениям сои.
8. Способ по любому из пп. 1-7, где выявление предусматривает амплификацию маркерного локуса или части маркерного локуса и выявление полученного в результате ампликона амплифицированного маркера.
9. Способ по п. 8, где амплификация включает: а) смешивание праймера для амплификации или пары праймеров для амплификации с нуклеиновой кислотой, выделенной из первых растения или идиоплазмы сои, где праймер или пара праймеров являются комплементарными или частично комплементарными по меньшей мере части маркерного локуса и способны инициировать полимеризацию ДНК с помощью ДНК-полимеразы при применении нуклеиновой кислоты сои в качестве матрицы; и b) удлинение праймера или пары праймеров в ходе реакции полимеризации ДНК, в которую включены ДНК-полимераза и нуклеиновая кислота-матрица, с образованием по меньшей мере одного ампликона.
10. Способ по п. 8 или п. 9, где амплификация включает использование полимеразной цепной реакции (ПЦР) или лигазной цепной реакции (ЛЦР) с применением нуклеиновой кислоты, выделенной из первых растения или идиоплазмы сои, качестве матрицы в ПЦР или ЛЦР.
11. Растение сои, отобранное или идентифицированное с помощью любого способов, описанных в предыдущих пунктах.
RU2017144616A 2015-05-20 2016-05-19 Растение с повышенным поглощением кремния RU2017144616A (ru)

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