RU2015139154A - Способы выделения вирусоподобных частиц - Google Patents
Способы выделения вирусоподобных частиц Download PDFInfo
- Publication number
- RU2015139154A RU2015139154A RU2015139154A RU2015139154A RU2015139154A RU 2015139154 A RU2015139154 A RU 2015139154A RU 2015139154 A RU2015139154 A RU 2015139154A RU 2015139154 A RU2015139154 A RU 2015139154A RU 2015139154 A RU2015139154 A RU 2015139154A
- Authority
- RU
- Russia
- Prior art keywords
- salt
- insect cells
- vlp
- cells
- group
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 15
- 239000002245 particle Substances 0.000 title claims 3
- 241000238631 Hexapoda Species 0.000 claims 8
- 150000003839 salts Chemical class 0.000 claims 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 241000700605 Viruses Species 0.000 claims 2
- 150000001768 cations Chemical class 0.000 claims 2
- 230000009089 cytolysis Effects 0.000 claims 2
- 230000006378 damage Effects 0.000 claims 2
- 238000002955 isolation Methods 0.000 claims 2
- 239000012266 salt solution Substances 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 2
- 241000701447 unidentified baculovirus Species 0.000 claims 2
- 241001533399 Circoviridae Species 0.000 claims 1
- 241000701945 Parvoviridae Species 0.000 claims 1
- 229910052783 alkali metal Inorganic materials 0.000 claims 1
- 150000001340 alkali metals Chemical class 0.000 claims 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 claims 1
- 150000001342 alkaline earth metals Chemical class 0.000 claims 1
- 150000001450 anions Chemical class 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 244000309711 non-enveloped viruses Species 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5258—Virus-like particles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14041—Use of virus, viral particle or viral elements as a vector
- C12N2710/14043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vectore
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/10011—Circoviridae
- C12N2750/10023—Virus like particles [VLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/10011—Circoviridae
- C12N2750/10051—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14023—Virus like particles [VLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14051—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14311—Parvovirus, e.g. minute virus of mice
- C12N2750/14323—Virus like particles [VLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14311—Parvovirus, e.g. minute virus of mice
- C12N2750/14351—Methods of production or purification of viral material
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Communicable Diseases (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Oncology (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Epidemiology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Gastroenterology & Hepatology (AREA)
Claims (15)
1. Способ выделения экспрессированных бакуловирусом вирусоподобных частиц (VLP) безоболочечных вирусов из клеток насекомых, отличающийся тем, что способ не включает стадий лизиса или разрушения клеток и включает стадию смешивания клеток насекомых с солью, где раствор соли в воде, полученный в результате смешивания, содержит по меньшей мере 300 мосмоль/л соли, катион которой выбран из группы, состоящей из щелочных металлов и щелочно-земельных металлов, и анион которой выбран из группы, состоящей из CI-, Br- и I-.
2. Способ по п. 1, отличающийся тем, что стадии смешивания клеток насекомых с солью предшествует стадия концентрации, во время которой клетки насекомых концентрируют.
3. Способ по п. 1, отличающийся тем, что катион выбран из группы, состоящей из Na+, Mg2+, Ca2+ и K+.
4. Способ по п. 3, отличающийся тем, что соль выбрана из группы, состоящей из NaCl, KC1, CaCl2 и MgCl2.
5. Способ по п. 1, отличающийся тем, что раствор соли в воде, полученный в результате смешивания, состоит, по существу, из соли согласно любому из п.п. 1-4 и воды.
6. Способ по п. 1, отличающийся тем, что безоболочечный вирус является членом семейства Parvoviridae или Circoviridae.
7. Способ по п. 1, отличающийся тем, что раствор соли в воде хранят при температуре 35-50ºC.
8. Способ по п. 7, отличающийся тем, что раствор соли в воде хранят при температуре 40-45ºC.
9. Способ по п. 1, отличающийся тем, что клетки насекомых представляют собой клетки SF9 или SF21.
10. Способ очистки экспрессированных бакуловирусами вирусоподобных частиц (VLP) безоболочечных вирусов, способ включает стадии
a) инфицирования клеток насекомых рекомбинантным бакуловирусом, кодирующим VLP-белки,
b) культивирования клеток насекомых,
c) выделения VLP из клеток насекомых,
d) отделения VLP,
отличающийся тем, что способ не включает стадий лизиса или разрушения клеток и стадия c) включает способ по любому из п.п. 1-9 для выделения VLP.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13155370.3 | 2013-02-15 | ||
| EP13155370 | 2013-02-15 | ||
| PCT/EP2014/052870 WO2014125053A1 (en) | 2013-02-15 | 2014-02-14 | Methods for the release of virus-like particles |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2015139154A true RU2015139154A (ru) | 2017-03-21 |
| RU2648143C2 RU2648143C2 (ru) | 2018-03-22 |
Family
ID=47749649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2015139154A RU2648143C2 (ru) | 2013-02-15 | 2014-02-14 | Способы выделения вирусоподобных частиц |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US9193759B2 (ru) |
| EP (1) | EP2956167B1 (ru) |
| JP (1) | JP6475641B2 (ru) |
| KR (1) | KR102071751B1 (ru) |
| CN (1) | CN105073131B (ru) |
| BR (1) | BR112015019619A2 (ru) |
| CA (1) | CA2899682C (ru) |
| ES (1) | ES2606040T3 (ru) |
| HU (1) | HUE031502T2 (ru) |
| MX (1) | MX354758B (ru) |
| RU (1) | RU2648143C2 (ru) |
| WO (1) | WO2014125053A1 (ru) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114250259A (zh) * | 2020-09-21 | 2022-03-29 | 成都天邦生物制品有限公司 | 一种猪圆环2型病毒Cap蛋白病毒样颗粒的发酵培养基 |
| WO2023127887A1 (ja) * | 2021-12-27 | 2023-07-06 | デンカ株式会社 | バキュロウイルス発現系におけるタンパク質の製造法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003213065A1 (en) | 2002-02-14 | 2003-09-04 | Novavax, Inc. | Novel insect cell line |
| EP2362901A1 (en) * | 2008-11-03 | 2011-09-07 | Ligocyte Pharmaceuticals, Inc. | Improved methods for isolating enveloped virus-based vlps free of infectious agents |
| US9580474B2 (en) | 2010-09-08 | 2017-02-28 | The Johns Hopkins University | Polyionic papilloma virus-like particle (VLP) vaccines |
-
2014
- 2014-02-10 US US14/176,249 patent/US9193759B2/en active Active
- 2014-02-14 CN CN201480008914.0A patent/CN105073131B/zh active Active
- 2014-02-14 EP EP14706516.3A patent/EP2956167B1/en not_active Revoked
- 2014-02-14 ES ES14706516.3T patent/ES2606040T3/es active Active
- 2014-02-14 HU HUE14706516A patent/HUE031502T2/en unknown
- 2014-02-14 KR KR1020157021812A patent/KR102071751B1/ko active IP Right Grant
- 2014-02-14 RU RU2015139154A patent/RU2648143C2/ru active
- 2014-02-14 JP JP2015557435A patent/JP6475641B2/ja active Active
- 2014-02-14 MX MX2015010566A patent/MX354758B/es active IP Right Grant
- 2014-02-14 WO PCT/EP2014/052870 patent/WO2014125053A1/en active Application Filing
- 2014-02-14 US US14/767,214 patent/US20150376577A1/en not_active Abandoned
- 2014-02-14 BR BR112015019619A patent/BR112015019619A2/pt not_active Application Discontinuation
- 2014-02-14 CA CA2899682A patent/CA2899682C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2016508720A (ja) | 2016-03-24 |
| CN105073131B (zh) | 2017-06-27 |
| MX2015010566A (es) | 2015-11-16 |
| WO2014125053A1 (en) | 2014-08-21 |
| BR112015019619A2 (pt) | 2017-07-18 |
| CA2899682C (en) | 2022-06-21 |
| EP2956167B1 (en) | 2016-10-19 |
| CA2899682A1 (en) | 2014-08-21 |
| KR102071751B1 (ko) | 2020-01-30 |
| US20150376577A1 (en) | 2015-12-31 |
| HUE031502T2 (en) | 2017-07-28 |
| CN105073131A (zh) | 2015-11-18 |
| KR20150116854A (ko) | 2015-10-16 |
| US9193759B2 (en) | 2015-11-24 |
| RU2648143C2 (ru) | 2018-03-22 |
| ES2606040T3 (es) | 2017-03-17 |
| US20140234900A1 (en) | 2014-08-21 |
| EP2956167A1 (en) | 2015-12-23 |
| MX354758B (es) | 2018-03-20 |
| JP6475641B2 (ja) | 2019-02-27 |
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