RU2012125624A - Варианты бета-глюкозидазы 1 с улучшенными свойствами - Google Patents
Варианты бета-глюкозидазы 1 с улучшенными свойствами Download PDFInfo
- Publication number
- RU2012125624A RU2012125624A RU2012125624/10A RU2012125624A RU2012125624A RU 2012125624 A RU2012125624 A RU 2012125624A RU 2012125624/10 A RU2012125624/10 A RU 2012125624/10A RU 2012125624 A RU2012125624 A RU 2012125624A RU 2012125624 A RU2012125624 A RU 2012125624A
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- Prior art keywords
- bgl1
- variant
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Links
- 101710099628 Beta-glucosidase 1 Proteins 0.000 title claims abstract 319
- 230000000694 effects Effects 0.000 claims abstract 120
- 230000007062 hydrolysis Effects 0.000 claims abstract 9
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract 9
- 102000006995 beta-Glucosidase Human genes 0.000 claims abstract 8
- 108010047754 beta-Glucosidase Proteins 0.000 claims abstract 8
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims abstract 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract 4
- 239000008103 glucose Substances 0.000 claims abstract 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract 3
- 229910021529 ammonia Inorganic materials 0.000 claims abstract 3
- 239000001913 cellulose Substances 0.000 claims abstract 3
- 229920002678 cellulose Polymers 0.000 claims abstract 3
- 235000005822 corn Nutrition 0.000 claims abstract 3
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract 3
- 102000004169 proteins and genes Human genes 0.000 claims abstract 3
- 108090000623 proteins and genes Proteins 0.000 claims abstract 3
- 239000010902 straw Substances 0.000 claims abstract 3
- 238000006467 substitution reaction Methods 0.000 claims 26
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 239000002028 Biomass Substances 0.000 claims 2
- 101100373011 Drosophila melanogaster wapl gene Proteins 0.000 claims 2
- 241000209149 Zea Species 0.000 claims 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 208000012396 long COVID-19 Diseases 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 210000004483 pasc Anatomy 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 235000000346 sugar Nutrition 0.000 claims 1
- 150000008163 sugars Chemical class 0.000 claims 1
- 240000008042 Zea mays Species 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26324009P | 2009-11-20 | 2009-11-20 | |
| US61/263,240 | 2009-11-20 | ||
| PCT/US2010/057531 WO2011063308A2 (en) | 2009-11-20 | 2010-11-19 | Beta-glucosidase i variants with improved properties |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2012125624A true RU2012125624A (ru) | 2013-12-27 |
Family
ID=43608030
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012125624/10A RU2012125624A (ru) | 2009-11-20 | 2010-11-19 | Варианты бета-глюкозидазы 1 с улучшенными свойствами |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US9447400B2 (cg-RX-API-DMAC7.html) |
| EP (6) | EP2602317B1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP2013511280A (cg-RX-API-DMAC7.html) |
| CN (2) | CN102844429B (cg-RX-API-DMAC7.html) |
| BR (1) | BR112012011711A2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2780767A1 (cg-RX-API-DMAC7.html) |
| DK (6) | DK2599863T3 (cg-RX-API-DMAC7.html) |
| IN (1) | IN2012DN03825A (cg-RX-API-DMAC7.html) |
| RU (1) | RU2012125624A (cg-RX-API-DMAC7.html) |
| WO (1) | WO2011063308A2 (cg-RX-API-DMAC7.html) |
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| JP7051491B2 (ja) * | 2018-02-26 | 2022-04-11 | 花王株式会社 | 変異β－グルコシダーゼ |
| JP2021516058A (ja) | 2018-03-09 | 2021-07-01 | ダニスコ・ユーエス・インク | グルコアミラーゼ及びその使用方法 |
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| US12343390B2 (en) | 2018-08-07 | 2025-07-01 | Wisconsin Alumni Research Foundation (Warf) | Recombinant biologically contained filovirus vaccine |
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| JP7569523B2 (ja) | 2018-11-20 | 2024-10-18 | インターナショナル エヌアンドエイチ デンマーク エーピーエス | 改変ロバスト高Ｔｍ－フィターゼ分岐群ポリペプチド及びその断片 |
| JP2022527235A (ja) * | 2019-01-23 | 2022-06-01 | 義裕 河岡 | インフルエンザウイルスにおける付加的遺伝子に遺伝的安定性を付与する突然変異 |
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| FR2935986B1 (fr) * | 2008-09-12 | 2012-11-23 | Inst Francais Du Petrole | Variants de beta-glucosidase a activite amelioree et leurs utilisations. |
| US8486683B2 (en) * | 2009-05-29 | 2013-07-16 | Iogen Energy Corporation | Beta-glucosidase enzymes |
| WO2012125937A2 (en) | 2011-03-17 | 2012-09-20 | Danisco Us Inc. | Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis |
| MX2013010512A (es) | 2011-03-17 | 2013-10-07 | Danisco Inc | Metodo para reducir la viscosidad en un proceso de sacarificacion. |
| US8468683B2 (en) * | 2011-10-31 | 2013-06-25 | HGST Netherlands B.V. | High bevel angle magnetic writer pole fabrication process |
| CA2888757A1 (en) | 2012-10-31 | 2014-05-08 | Danisco Us Inc. | Compositions and methods of use |
| CA2889193A1 (en) | 2012-10-31 | 2014-05-08 | Danisco Us Inc. | Beta-glucosidase from neurospora crassa |
| CN104781399A (zh) | 2012-10-31 | 2015-07-15 | 丹尼斯科美国公司 | 来自稻瘟病菌的β-葡糖苷酶 |
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2010
- 2010-11-19 JP JP2012540120A patent/JP2013511280A/ja active Pending
- 2010-11-19 BR BR112012011711A patent/BR112012011711A2/pt not_active IP Right Cessation
- 2010-11-19 IN IN3825DEN2012 patent/IN2012DN03825A/en unknown
- 2010-11-19 EP EP12199328.1A patent/EP2602317B1/en not_active Not-in-force
- 2010-11-19 CN CN201080061975.5A patent/CN102844429B/zh not_active Expired - Fee Related
- 2010-11-19 CA CA2780767A patent/CA2780767A1/en not_active Abandoned
- 2010-11-19 US US13/510,902 patent/US9447400B2/en not_active Expired - Fee Related
- 2010-11-19 EP EP12199332.3A patent/EP2599864B1/en not_active Not-in-force
- 2010-11-19 CN CN201510672711.8A patent/CN105316303B/zh not_active Expired - Fee Related
- 2010-11-19 EP EP12199330.7A patent/EP2599863B1/en not_active Not-in-force
- 2010-11-19 DK DK12199330.7T patent/DK2599863T3/da active
- 2010-11-19 RU RU2012125624/10A patent/RU2012125624A/ru not_active Application Discontinuation
- 2010-11-19 EP EP12199329.9A patent/EP2599862B1/en not_active Not-in-force
- 2010-11-19 DK DK10782511.9T patent/DK2501806T3/da active
- 2010-11-19 DK DK12199329.9T patent/DK2599862T3/da active
- 2010-11-19 EP EP12199327.3A patent/EP2599861B1/en not_active Not-in-force
- 2010-11-19 DK DK12199328.1T patent/DK2602317T3/da active
- 2010-11-19 DK DK12199327.3T patent/DK2599861T3/da active
- 2010-11-19 EP EP10782511.9A patent/EP2501806B1/en not_active Not-in-force
- 2010-11-19 DK DK12199332.3T patent/DK2599864T3/da active
- 2010-11-19 WO PCT/US2010/057531 patent/WO2011063308A2/en not_active Ceased
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2015
- 2015-06-16 JP JP2015120937A patent/JP2015211678A/ja not_active Withdrawn
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2016
- 2016-08-29 US US15/250,626 patent/US20170145396A1/en not_active Abandoned
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