RU2012108029A - METHOD FOR DIAGNOSTIC OF INFECTIOUS NECROSIS VIRUS OF Pancreas by Salmon Method by Polymerase Chain Reaction - Google Patents
METHOD FOR DIAGNOSTIC OF INFECTIOUS NECROSIS VIRUS OF Pancreas by Salmon Method by Polymerase Chain Reaction Download PDFInfo
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- RU2012108029A RU2012108029A RU2012108029/15A RU2012108029A RU2012108029A RU 2012108029 A RU2012108029 A RU 2012108029A RU 2012108029/15 A RU2012108029/15 A RU 2012108029/15A RU 2012108029 A RU2012108029 A RU 2012108029A RU 2012108029 A RU2012108029 A RU 2012108029A
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- necrosis virus
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- chain reaction
- polymerase chain
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Abstract
Способ диагностики вируса инфекционного некроза поджелудочной железы лососевых методом полимеразной цепной реакции, включающий два прямых и два обратных олигонуклеотидных праймера для выявления фрагмента сегмента В, кодирующего РНК-зависимую РНК-полимеразу вируса инфекционного некроза поджелудочной железы с электрофоретическим определением размера амплифицируемого фрагмента нуклеотидной последовательности, отличающийся тем, что используют в качестве праймеров олигонуклеотиды следующей структуры - В37: ACGTTGGTGGCACCCGACATAC, В123: TGTCGGACATCTTCAACTCACC, B320r: CAGTCGAGGCAGAGCGGCATC, B853r: GTGTCTCCTTTGGTTTTGCCTATG, имеющие следующие характеристики: отсутствие самокомплементарных участков внутри каждого праймера и между прямыми и обратными праймерами, фланкируют фрагменты размером 860 и 240 пар нуклеотидов гена, кодирующего РНК-зависимую РНК-полимеразу вируса инфекционного некроза поджелудочной железы лососевых.A method for the diagnosis of salmon infectious pancreatic necrosis virus by polymerase chain reaction, including two direct and two reverse oligonucleotide primers for detecting a segment B fragment encoding RNA-dependent RNA polymerase of the pancreatic necrosis virus with electrophoretic determination of the size of the amplified fragment of the nucleotide sequence that oligonucleotides of the following structure are used as primers - B37: ACGTTGGTGGCACCCGACATAC, B123: TGTCGGACATCTTCAACT CACC, B320r: CAGTCGAGGCAGAGCGGCATC, B853r: GTGTCTCCTTTGGTTTTGCCTATG, having the following characteristics: the absence of self-complementary regions within each primer and between the forward and reverse primers, flank fragments of 860 and 240 pairs of the nucleic acid RNA gene .
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RU2012108029/15A RU2508547C2 (en) | 2012-03-05 | 2012-03-05 | Diagnostic technique for pactreatic infectious necrosis virus in salmon by polymerase chain reaction |
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RU2012108029/15A RU2508547C2 (en) | 2012-03-05 | 2012-03-05 | Diagnostic technique for pactreatic infectious necrosis virus in salmon by polymerase chain reaction |
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RU2012108029A true RU2012108029A (en) | 2013-09-10 |
RU2508547C2 RU2508547C2 (en) | 2014-02-27 |
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RU2012108029/15A RU2508547C2 (en) | 2012-03-05 | 2012-03-05 | Diagnostic technique for pactreatic infectious necrosis virus in salmon by polymerase chain reaction |
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US20090181363A1 (en) * | 2005-12-21 | 2009-07-16 | Dhar Arun K | Non-invasive detection of fish viruses by real-time pcr |
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