RU2008129928A - METHOD FOR PREPARING BACTERIAL FERTILIZER "STIM" BASED ON BACTERIA OF THE GENUS AZOTOBACTER - Google Patents
METHOD FOR PREPARING BACTERIAL FERTILIZER "STIM" BASED ON BACTERIA OF THE GENUS AZOTOBACTER Download PDFInfo
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- RU2008129928A RU2008129928A RU2008129928/13A RU2008129928A RU2008129928A RU 2008129928 A RU2008129928 A RU 2008129928A RU 2008129928/13 A RU2008129928/13 A RU 2008129928/13A RU 2008129928 A RU2008129928 A RU 2008129928A RU 2008129928 A RU2008129928 A RU 2008129928A
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- azotobacter
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1. Способ приготовления бактериального удобрения «Стим» на основе бактерии рода Azotobacter, включающий культивирование бактерий на плотной среде Эшби, отличающийся тем, что предварительно из жидких элективных культур выделяют свободные аэробные азотофиксаторы с последующим их культивированием в чистые культуры на плотной питательной среде, из чистых культур отбирают наиболее активно развивающиеся штаммы и переносят их в жидкую питательную среду, не содержащую азот, в полученную устойчивую бактериальную суспензию, стимулирующую рост сельскохозяйственных растений, добавляют микробиологический препарат на основе бактерий рода Псеводомонад и почвенный фильтрат, все тщательно перемешивают, затем добавляют профильтрованную среду Эшби для азотобактера и олигонитрофилов с раствором микроэлементов по Федорову и осуществляют культивирование в ферментере. ! 2. Способ по п.1, отличающийся тем, что почвенный фильтрат представляет собой жидкость, полученную после фильтрации надосадочной части раствора черноземной почвы в воде, через бумажный фильтр и фильтр Беркефельда. ! 3. Способ по п.1, отличающийся тем, что культивирование в ферментере осуществляют при температуре 22-28°С при скорости вращения мешалки 180 об/мин в течение 10 суток, 4 раза в сутки по 2 ч.1. The method of preparation of the bacterial fertilizer "Steam" based on bacteria of the genus Azotobacter, including the cultivation of bacteria on a solid Ashby medium, characterized in that previously free aerobic nitrogen fixers are isolated from liquid elective cultures, followed by their cultivation in pure cultures on a dense nutrient medium, from pure cultures, the most actively developing strains are selected and transferred to a liquid nutrient-free medium containing no nitrogen, to the resulting stable bacterial suspension, which stimulates the growth of organic plants, add a microbiological preparation based on bacteria of the genus Pseudodomonas and soil filtrate, mix everything thoroughly, then add Ashby filtered medium for azotobacter and oligonitrophils with a solution of trace elements according to Fedorov and carry out cultivation in a fermenter. ! 2. The method according to claim 1, characterized in that the soil filtrate is a liquid obtained after filtering the supernatant of a solution of chernozem soil in water through a paper filter and a Berkefeld filter. ! 3. The method according to claim 1, characterized in that the cultivation in the fermenter is carried out at a temperature of 22-28 ° C at a speed of rotation of the stirrer 180 rpm for 10 days, 4 times a day for 2 hours
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008129928/13A RU2390517C2 (en) | 2008-07-23 | 2008-07-23 | Method of making bacterial fertiliser based on azotobacter bacteria genus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008129928/13A RU2390517C2 (en) | 2008-07-23 | 2008-07-23 | Method of making bacterial fertiliser based on azotobacter bacteria genus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2008129928A true RU2008129928A (en) | 2010-01-27 |
| RU2390517C2 RU2390517C2 (en) | 2010-05-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2008129928/13A RU2390517C2 (en) | 2008-07-23 | 2008-07-23 | Method of making bacterial fertiliser based on azotobacter bacteria genus |
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| Country | Link |
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| RU (1) | RU2390517C2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3892716A1 (en) | 2020-04-07 | 2021-10-13 | Biofor System d.o.o. | Novel plant growth promoting bacterial strains, an improved method for encapsulation of bacteria and a biofertilizer comprising encapsulated bacteria prepared by the said method |
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2008
- 2008-07-23 RU RU2008129928/13A patent/RU2390517C2/en not_active IP Right Cessation
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3892716A1 (en) | 2020-04-07 | 2021-10-13 | Biofor System d.o.o. | Novel plant growth promoting bacterial strains, an improved method for encapsulation of bacteria and a biofertilizer comprising encapsulated bacteria prepared by the said method |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2390517C2 (en) | 2010-05-27 |
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| Date | Code | Title | Description |
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| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20110724 |