RU2004116319A - Система регулируемой экспрессии нуклеиновых кислот - Google Patents
Система регулируемой экспрессии нуклеиновых кислот Download PDFInfo
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
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- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
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Claims (20)
1. Упаковывающая клетка, содержащая первую, вторую и третью конструкции нуклеиновых кислот, которые регулируют экспрессию одного или нескольких вирусных генных продуктов, необходимых для упаковки вирусного вектора, где
указанная первая конструкция нуклеиновой кислоты содержит регулируемый тетрациклином промотор/оператор, который регулирует экспрессию кодируемого первого продукта;
указанный первый продукт способен регулировать экспрессию второго продукта, кодируемого указанной второй конструкцией нуклеиновой кислоты; и
указанный второй продукт способен регулировать экспрессию указанного вирусного генного продукта, кодируемого последовательностью, присутствующей на указанной третьей конструкции нуклеиновой кислоты.
2. Клетка по п.1, где указанным первым продуктом является трансактиватор регулируемого тетрациклином промотора/оператора или гибридный белок, включающий указанный трансактиватор.
3. Клетка по п.1 или 2, где указанная вторая конструкция нуклеиновой кислоты содержит регулируемый тетрациклином промотор/оператор.
4. Клетка по п.1 или 2, где указанным вторым продуктом является белок rev.
5. Клетка по п.1 или 2, где указанная третья конструкция содержит промотор, происходящий от ретровирусного 5’LTR.
6. Клетка по п.1 или 2, где указанным первым продуктом является белок tat или химерный белок, включающий белок tat.
7. Клетка по п.1 или 2, которая является стабильно трансфицированной указанными конструкциями нуклеиновых кислот.
8. Клетка по п.1 или 2, которая, кроме того, содержит вирусный вектор, реплицирующийся в зависимости от условий и которая упаковывает указанный вектор.
9. Клетка по п.8, где указанный вектор происходит от ВИЧ-1.
10. Клетка по п.1, где указанным вирусным генным продуктом является белок вирусной оболочки или G-белок.
11. Клетка по п.10, которая, кроме того, содержит дополнительную конструкцию нуклеиновой кислоты, кодирующую ретровирусные белки gag и pol.
12. Клетка по п.10, где указанным вторым продуктом является белок rev.
13. Клетка по п.10, где указанным вирусным генным продуктом является G-белок.
14. Клетка по п.10, которая, кроме того, содержит условно реплицирующийся вирусный вектор и которая упаковывает указанный вектор.
15. Упаковывающая клетка, содержащая первую, вторую и третью конструкции нуклеиновых кислот, которые регулируют экспрессию одного или нескольких белков вирусной оболочки или G-белков, где
указанная первая конструкция нуклеиновой кислоты способна экспрессировать кодируемый первый продукт;
указанный первый продукт способен регулировать экспрессию второго продукта, кодируемого указанной второй конструкцией нуклеиновой кислоты; и
указанный второй продукт способен регулировать экспрессию указанного белка вирусной оболочки или G-белка, кодируемого последовательностью, присутствующей на указанной третьей конструкции нуклеиновой кислоты.
16. Клетка по п.15, которая, кроме того, содержит дополнительную конструкцию нуклеиновой кислоты, кодирующую ретровирусные белки gag и pol.
17. Клетка по п.15, где указанным вирусным генным продуктом является G-белок.
18. Клетка по п.17, где указанным G-белком является G-белок вируса VSV или Мокола.
19. Способ упаковки вирусного вектора, предусматривающий культивирование клетки по п.9 в условиях, при которых указанная первая конструкция нуклеиновой кислоты экспрессирует указанный первый продукт.
20. Способ упаковки вирусного вектора, предусматривающий культивирование клетки по п.14 в условиях, при которых указанная первая конструкция нуклеиновой кислоты экспрессирует указанный первый продукт.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/020,472 | 2001-10-30 | ||
US10/020,472 US6835568B2 (en) | 2001-10-30 | 2001-10-30 | Regulated nucleic acid expression system |
Publications (1)
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RU2004116319A true RU2004116319A (ru) | 2005-03-27 |
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RU2004116319/13A RU2004116319A (ru) | 2001-10-30 | 2002-10-30 | Система регулируемой экспрессии нуклеиновых кислот |
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Country | Link |
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US (2) | US6835568B2 (ru) |
EP (1) | EP1446488A4 (ru) |
JP (1) | JP2005512573A (ru) |
KR (1) | KR100984268B1 (ru) |
CN (1) | CN100591769C (ru) |
AU (1) | AU2002365090B2 (ru) |
BR (1) | BR0213964A (ru) |
CA (1) | CA2464268C (ru) |
CZ (1) | CZ2004546A3 (ru) |
IL (2) | IL161495A0 (ru) |
MX (1) | MXPA04003969A (ru) |
NO (1) | NO20041763L (ru) |
NZ (1) | NZ533132A (ru) |
RU (1) | RU2004116319A (ru) |
WO (1) | WO2003054210A2 (ru) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7297536B2 (en) * | 2003-01-23 | 2007-11-20 | Wisconsin Alumni Research Foundation | Inducible protein expression system |
US20090123494A1 (en) * | 2007-07-31 | 2009-05-14 | William Staplin | Momlv-based pseudovirion packaging cell line |
JP2011517409A (ja) * | 2008-03-28 | 2011-06-09 | バイレクシス コーポレイション | レンチウイルスベースの免疫原性ベクター |
EP2497830A1 (en) * | 2009-11-05 | 2012-09-12 | Proyecto de Biomedicina Cima, S.L. | Regulated expression systems |
US20130189754A1 (en) | 2011-09-12 | 2013-07-25 | International Aids Vaccine Initiative | Immunoselection of recombinant vesicular stomatitis virus expressing hiv-1 proteins by broadly neutralizing antibodies |
US9402894B2 (en) | 2011-10-27 | 2016-08-02 | International Aids Vaccine Initiative | Viral particles derived from an enveloped virus |
US9347065B2 (en) | 2012-03-29 | 2016-05-24 | International Aids Vaccine Initiative | Methods to improve vector expression and genetic stability |
US10450574B2 (en) * | 2015-11-24 | 2019-10-22 | Glaxosmithkline Intellectual Property Development Limited | Transient transfection method for retroviral production |
CA3006288A1 (en) * | 2015-11-24 | 2017-06-01 | Glaxosmithkline Intellectual Property Development Limited | Stable cell lines for retroviral production |
AU2018356538B2 (en) * | 2017-10-25 | 2023-09-28 | Nouscom Ag | Eukaryotic cell line |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
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EP0449116B2 (en) | 1990-03-21 | 2004-08-25 | Geneart GmbH | DNA sequences encoding modified retroviral gag polypeptides and vaccines containing them or aggregates thereof |
US6174666B1 (en) | 1992-03-27 | 2001-01-16 | The United States Of America As Represented By The Department Of Health And Human Services | Method of eliminating inhibitory/instability regions from mRNA |
US5750396A (en) | 1995-05-08 | 1998-05-12 | St. Judes Children's Research Hospital | Stable virus packaging cell lines |
US5888767A (en) | 1996-11-27 | 1999-03-30 | The Johns Hopkins University School Of Medicine | Method of using a conditionally replicating viral vector to express a gene |
US5885806A (en) | 1995-11-28 | 1999-03-23 | The Johns Hopkins University School Of Medicine | Methods to prepare conditionally replicating viral vectors |
US6133027A (en) * | 1996-08-07 | 2000-10-17 | City Of Hope | Inducible expression system |
GB9803351D0 (en) | 1998-02-17 | 1998-04-15 | Oxford Biomedica Ltd | Anti-viral vectors |
AU778106B2 (en) | 1999-04-26 | 2004-11-18 | K.U. Leuven Research & Development | Synthetic gene for expressing active retroviral protein in eukaryotes |
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2001
- 2001-10-30 US US10/020,472 patent/US6835568B2/en not_active Expired - Fee Related
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2002
- 2002-10-30 NZ NZ533132A patent/NZ533132A/en not_active IP Right Cessation
- 2002-10-30 WO PCT/US2002/034932 patent/WO2003054210A2/en not_active Application Discontinuation
- 2002-10-30 MX MXPA04003969A patent/MXPA04003969A/es active IP Right Grant
- 2002-10-30 CA CA2464268A patent/CA2464268C/en not_active Expired - Fee Related
- 2002-10-30 AU AU2002365090A patent/AU2002365090B2/en not_active Ceased
- 2002-10-30 EP EP20020802938 patent/EP1446488A4/en not_active Withdrawn
- 2002-10-30 JP JP2003554911A patent/JP2005512573A/ja active Pending
- 2002-10-30 CZ CZ2004546A patent/CZ2004546A3/cs unknown
- 2002-10-30 CN CN02821442A patent/CN100591769C/zh not_active Expired - Fee Related
- 2002-10-30 RU RU2004116319/13A patent/RU2004116319A/ru not_active Application Discontinuation
- 2002-10-30 KR KR1020047006464A patent/KR100984268B1/ko not_active IP Right Cessation
- 2002-10-30 BR BRPI0213964-2A patent/BR0213964A/pt not_active Application Discontinuation
- 2002-10-30 IL IL16149502A patent/IL161495A0/xx unknown
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2004
- 2004-04-19 IL IL161495A patent/IL161495A/en not_active IP Right Cessation
- 2004-04-29 NO NO20041763A patent/NO20041763L/no not_active Application Discontinuation
- 2004-12-13 US US11/011,264 patent/US8043611B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
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EP1446488A2 (en) | 2004-08-18 |
NZ533132A (en) | 2008-04-30 |
US20030087419A1 (en) | 2003-05-08 |
EP1446488A4 (en) | 2004-12-22 |
US20060121579A1 (en) | 2006-06-08 |
AU2002365090B2 (en) | 2007-12-20 |
NO20041763L (no) | 2004-04-29 |
WO2003054210A3 (en) | 2003-12-24 |
CN100591769C (zh) | 2010-02-24 |
US6835568B2 (en) | 2004-12-28 |
IL161495A0 (en) | 2004-09-27 |
AU2002365090A1 (en) | 2003-07-09 |
KR100984268B1 (ko) | 2010-09-30 |
IL161495A (en) | 2009-06-15 |
US8043611B2 (en) | 2011-10-25 |
CN1578837A (zh) | 2005-02-09 |
JP2005512573A (ja) | 2005-05-12 |
BR0213964A (pt) | 2006-05-30 |
KR20040066799A (ko) | 2004-07-27 |
CZ2004546A3 (cs) | 2004-10-13 |
MXPA04003969A (es) | 2004-07-08 |
WO2003054210B1 (en) | 2004-02-26 |
CA2464268A1 (en) | 2003-07-03 |
CA2464268C (en) | 2012-03-20 |
WO2003054210A2 (en) | 2003-07-03 |
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