RU2003108441A - METHOD FOR PRODUCING L-AMINO ACID - Google Patents

METHOD FOR PRODUCING L-AMINO ACID

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Publication number
RU2003108441A
RU2003108441A RU2003108441/13A RU2003108441A RU2003108441A RU 2003108441 A RU2003108441 A RU 2003108441A RU 2003108441/13 A RU2003108441/13 A RU 2003108441/13A RU 2003108441 A RU2003108441 A RU 2003108441A RU 2003108441 A RU2003108441 A RU 2003108441A
Authority
RU
Russia
Prior art keywords
amino acid
activity
acid
phosphogluconataldolase
deoxy
Prior art date
Application number
RU2003108441/13A
Other languages
Russian (ru)
Other versions
RU2307165C2 (en
Inventor
Ёсихико ХАРА
Хироси ИЗУИ
Такахиро АСАНО
Ясуюки ВАТАНАБЕ
Цуёси НАКАМАЦУ
Original Assignee
Адзиномото Ко., Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2002088668A external-priority patent/JP3932945B2/en
Application filed by Адзиномото Ко., Инк. filed Critical Адзиномото Ко., Инк.
Publication of RU2003108441A publication Critical patent/RU2003108441A/en
Application granted granted Critical
Publication of RU2307165C2 publication Critical patent/RU2307165C2/en

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Claims (7)

1. Способ получения L-аминокислоты, включающий культивирование микроорганизма, обладающего способностью продуцировать L-аминокислоту, в среде, чтобы продуцировать и накопить L-аминокислоту в среде, и сбор L-аминокислоты из среды, где микроорганизм является грамотрицательной бактерией, имеющей путь Энтнера-Дудорова, которая была модифицирована так, что активность 6-фосфоглюконатдегидратазы или активность 2-кето-3-дезокси-6-фосфоглюконатальдолазы, или активности обоих ферментов усилены, и L-аминокислота выбрана из L-аминокислот, получаемых путем биосинтеза с использованием в качестве промежуточного соединения пировиноградной кислоты.1. A method for producing an L-amino acid, comprising cultivating a microorganism having the ability to produce an L-amino acid in a medium to produce and accumulate an L-amino acid in a medium, and collecting the L-amino acid from a medium where the microorganism is a gram-negative bacterium having an Entner-pathway Dudorov, which has been modified so that the activity of 6-phosphogluconate dehydratase or the activity of 2-keto-3-deoxy-6-phosphogluconataldolase, or the activity of both enzymes is enhanced, and the L-amino acid is selected from L-amino acids obtained by the biosynthesis using as intermediate pyruvic acid. 2. Способ по п.1, где бактерия является энтеробактерией.2. The method according to claim 1, where the bacterium is an enterobacterium. 3. Способ по п.2, где бактерия относится к роду Enterobacter.3. The method according to claim 2, where the bacterium belongs to the genus Enterobacter. 4. Способ по любому из пп.1-3, где активность 6-фосфоглюконатдегидратазы или активность 2-кето-3-дезокси-6-фосфоглюконатальдолазы усилена посредством увеличения количества копий гена, кодирующего 6-фосфоглюконатдегидратазу или 2-кето-3-дезокси-6-фосфоглюконатальдолазу, или посредством модифицирования последовательности, регулирующей экспрессию гена, так что экспрессия гена в клетке бактерии усилена.4. The method according to any one of claims 1 to 3, wherein the activity of 6-phosphogluconate dehydratase or the activity of 2-keto-3-deoxy-6-phosphogluconataldolase is enhanced by increasing the number of copies of the gene encoding 6-phosphogluconate dehydratase or 2-keto-3-deoxy- 6-phosphogluconataldolase, or by modifying the sequence that regulates gene expression, so that gene expression in the bacterial cell is enhanced. 5. Способ по п.1, где L-аминокислотой является L-глутаминовая кислота или L-аминокислота образуется путем биосинтеза с использованием L-глутаминовой кислоты в качестве промежуточного соединения или донора аминогруппы.5. The method according to claim 1, where the L-amino acid is L-glutamic acid or an L-amino acid is formed by biosynthesis using L-glutamic acid as an intermediate or an amino group donor. 6. Способ по любому из пп.1-5, где L-аминокислота выбрана из L-глутаминовой кислоты, L-аргинина, L-глутамина, L-пролина, L-лейцина, L-изолейцина, L-валина и L-аланина.6. The method according to any one of claims 1 to 5, where the L-amino acid is selected from L-glutamic acid, L-arginine, L-glutamine, L-proline, L-leucine, L-isoleucine, L-valine and L-alanine . 7. Способ по п.6, где L-аминокислотой является L-глутаминовая кислота.7. The method according to claim 6, where the L-amino acid is L-glutamic acid.
RU2003108441/13A 2002-03-27 2003-03-26 Method for production of l-amino acid RU2307165C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002-88668 2002-03-27
JP2002088668A JP3932945B2 (en) 2002-03-27 2002-03-27 Method for producing L-amino acid

Publications (2)

Publication Number Publication Date
RU2003108441A true RU2003108441A (en) 2004-10-27
RU2307165C2 RU2307165C2 (en) 2007-09-27

Family

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Family Applications (1)

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RU2003108441/13A RU2307165C2 (en) 2002-03-27 2003-03-26 Method for production of l-amino acid

Country Status (16)

Country Link
US (2) US7037690B2 (en)
EP (1) EP1352966B1 (en)
JP (1) JP3932945B2 (en)
KR (1) KR100949048B1 (en)
CN (1) CN100529094C (en)
AT (1) ATE299945T1 (en)
AU (1) AU2003202509B2 (en)
BR (1) BR0300978B1 (en)
DE (1) DE60301031T2 (en)
DK (1) DK1352966T3 (en)
ES (1) ES2243826T3 (en)
MY (1) MY130526A (en)
PE (1) PE20031014A1 (en)
PL (1) PL359383A1 (en)
RU (1) RU2307165C2 (en)
TW (1) TWI308594B (en)

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