WO2005103228B1 - L-amino acid-producing bacterium and a method for producing l-amino acid - Google Patents
L-amino acid-producing bacterium and a method for producing l-amino acidInfo
- Publication number
- WO2005103228B1 WO2005103228B1 PCT/JP2005/008402 JP2005008402W WO2005103228B1 WO 2005103228 B1 WO2005103228 B1 WO 2005103228B1 JP 2005008402 W JP2005008402 W JP 2005008402W WO 2005103228 B1 WO2005103228 B1 WO 2005103228B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- strain
- amino acid
- producing
- valine
- tryptophan
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title claims 2
- 150000008575 L-amino acids Chemical class 0.000 title abstract 3
- 241000894006 Bacteria Species 0.000 title 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims abstract 8
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims abstract 8
- 229960004799 Tryptophan Drugs 0.000 claims abstract 4
- 229960004295 valine Drugs 0.000 claims abstract 4
- 241000588724 Escherichia coli Species 0.000 claims abstract 2
- 241001646716 Escherichia coli K-12 Species 0.000 claims abstract 2
- 101700047309 ilvB2 Proteins 0.000 claims 2
- 108010000700 EC 2.2.1.6 Proteins 0.000 claims 1
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 1
- 230000001580 bacterial Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000002708 enhancing Effects 0.000 claims 1
- 230000003834 intracellular Effects 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 abstract 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 abstract 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 abstract 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 abstract 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 abstract 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 abstract 2
- 229960002433 Cysteine Drugs 0.000 abstract 1
- 229960002989 Glutamic Acid Drugs 0.000 abstract 1
- 229960002885 Histidine Drugs 0.000 abstract 1
- 235000019766 L-Lysine Nutrition 0.000 abstract 1
- 235000013878 L-cysteine Nutrition 0.000 abstract 1
- 239000004201 L-cysteine Substances 0.000 abstract 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 abstract 1
- 239000004472 Lysine Substances 0.000 abstract 1
- 229960005190 Phenylalanine Drugs 0.000 abstract 1
- 229960002429 Proline Drugs 0.000 abstract 1
- 229960004441 Tyrosine Drugs 0.000 abstract 1
- 229960003646 lysine Drugs 0.000 abstract 1
Abstract
An L-amino acid-producing strain of Escherichia coli is bred by modifying an Escherichia coli K12 strain or a derivative thereof so as to become resistant to L-valine and have an ability to produce one or more L-amino acids selected from the group consisting of L-tryptophan, L-phenylalanine, L-lysine, L-tyrosine, L-glutamic acid, L-histidine, L-cysteine, and L-proline.
Claims
1. An isolated strain of Escherichia coli obtainable by modifying an Escherichia coli K12 strain or a derivative thereof, wherein said isolated strain is resistant to L-valine and has an ability to produce L-tryptophan.
2. The strain according to claim 1, wherein said strain has an ability to grow in a medium containing 20 mg/L of L-valine.
3. The strain according to claim 1, wherein said isolated strain is modified to be L-valine-resistant by enhancing an intracellular acetohydroxy acid synthase activity so that it is higher than that of a non-modified Kl 2 strain.
4. The isolated strain according to claim 3, wherein said isolated strain is modified so as to produce active acetohydroxy acid synthase II.
5. The isolated strain according to claim 4, wherein said active acetohydroxy acid synthase II is a protein encoded by a gene comprising a nucleotide sequence of SEQ ID NO: 3 or 5.
6. A method for producing L-tryptophan, comprising culturing the strain as defined in any one of claims 1 to 5 in a medium, and collecting L-tryptophan from the medium or bacterial cells.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT05737046T ATE557096T1 (en) | 2004-04-26 | 2005-04-26 | L-TRYPTOPHAN PRODUCING BACTERIA AND METHOD FOR PRODUCING L-TRYPTOPHAN |
| DK05737046.2T DK1740694T3 (en) | 2004-04-26 | 2005-04-26 | L-tryptophan-producing bacterium and method for producing L-tryptophan |
| CN2005800200952A CN1969038B (en) | 2004-04-26 | 2005-04-26 | L-amino acid-producing bacterium and method for producing L-amino acid |
| EP05737046A EP1740694B1 (en) | 2004-04-26 | 2005-04-26 | L-tryptophan-producing bacterium and a method for producing l-tryptophan |
| BRPI0509982A BRPI0509982B1 (en) | 2004-04-26 | 2005-04-26 | method for producing l-tryptophan |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004-130088 | 2004-04-26 | ||
| JP2004130088 | 2004-04-26 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO2005103228A2 WO2005103228A2 (en) | 2005-11-03 |
| WO2005103228A3 WO2005103228A3 (en) | 2006-03-02 |
| WO2005103228B1 true WO2005103228B1 (en) | 2006-04-20 |
Family
ID=34966388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP2005/008402 WO2005103228A2 (en) | 2004-04-26 | 2005-04-26 | L-amino acid-producing bacterium and a method for producing l-amino acid |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7300776B2 (en) |
| EP (1) | EP1740694B1 (en) |
| CN (1) | CN1969038B (en) |
| AT (1) | ATE557096T1 (en) |
| BR (1) | BRPI0509982B1 (en) |
| DK (1) | DK1740694T3 (en) |
| WO (1) | WO2005103228A2 (en) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4380305B2 (en) * | 2003-11-21 | 2009-12-09 | 味の素株式会社 | Method for producing L-amino acid by fermentation |
| PL1664318T3 (en) * | 2004-01-30 | 2010-03-31 | Ajinomoto Kk | L-amino acid-producing microorganism and method for producing l-amino acid |
| US7344874B2 (en) * | 2004-03-04 | 2008-03-18 | Ajinomoto Co., Inc. | L-glutamic acid-producing microorganism and a method for producing L-glutamic acid |
| US7482140B2 (en) * | 2004-06-15 | 2009-01-27 | Ajinomoto Co., Inc. | L-tyrosine-producing bacterium and a method for producing L-tyrosine |
| US7205132B2 (en) * | 2004-09-10 | 2007-04-17 | Ajinomoto Co., Inc. | L-glutamic acid-producing microorganism and a method for producing L-glutamic acid |
| US7794989B2 (en) | 2004-12-28 | 2010-09-14 | Ajinomoto Co., Inc. | L-glutamic acid-producing microorganism and a method for producing L-glutamic acid |
| US7547531B2 (en) | 2005-01-18 | 2009-06-16 | Ajinomoto Co., Inc. | L-amino acid producing microorganism which has been modified to inactive the fimH gene, and a method for producing I-amino acid |
| JP2008283863A (en) | 2005-08-26 | 2008-11-27 | Ajinomoto Co Inc | L-amino acid-producing bacterium and method for producing l-amino acid |
| JP2009060791A (en) * | 2006-03-30 | 2009-03-26 | Ajinomoto Co Inc | L-amino acid-producing bacterium and method for producing l-amino acid |
| RU2006129690A (en) | 2006-08-16 | 2008-02-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | METHOD FOR PRODUCING L-AMINO ACID USING BACTERIA OF THE Enterobacteriaceae FAMILY IN WHICH EXPRESSION OF THE ydiN GENE, ydiB GENE OR THEIR COMBINATION IS DECREASED |
| JP2010017081A (en) * | 2006-10-10 | 2010-01-28 | Ajinomoto Co Inc | Method for producing l-amino acid |
| JP2010017082A (en) * | 2006-10-10 | 2010-01-28 | Ajinomoto Co Inc | Method for producing l-amino acid |
| JP5217780B2 (en) * | 2008-02-08 | 2013-06-19 | 味の素株式会社 | Microorganism producing L-amino acid and method for producing L-amino acid |
| ES2510865T3 (en) | 2008-03-03 | 2014-10-21 | Global Bio-Chem Technology Group Company Limited | Recombinant microorganism and procedure to produce L-lysine |
| KR101261147B1 (en) * | 2011-01-18 | 2013-05-06 | 씨제이제일제당 (주) | A microorganism having enhanced l-amino acids productivity and process for producing l-amino acids using the same |
| JP6545676B2 (en) | 2013-11-14 | 2019-07-17 | スカラブ ゲノミクス， エルエルシー | Bacteria with improved metabolic capacity |
| JP6473460B2 (en) * | 2014-03-03 | 2019-02-20 | スカラブ ゲノミクス， エルエルシー | Enhanced production of recombinant CRM197 in E. coli |
| CN109868254B (en) * | 2019-03-14 | 2021-02-19 | 浙江工业大学 | Genetically engineered bacterium for high yield of pantothenic acid, construction method and application |
| CN112779203B (en) * | 2021-01-19 | 2022-12-30 | 浙江工业大学 | Genetically engineered bacterium for high yield of L-cysteine and construction and application thereof |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4946917B1 (en) * | 1970-12-22 | 1974-12-12 | ||
| JPS61264238A (en) | 1985-05-17 | 1986-11-22 | Asahi Eng Kk | Light-transmissive type lubricant abnormality detector |
| US5976843A (en) * | 1992-04-22 | 1999-11-02 | Ajinomoto Co., Inc. | Bacterial strain of Escherichia coli BKIIM B-3996 as the producer of L-threonine |
| DE69124939T2 (en) | 1990-11-30 | 1997-10-02 | Ajinomoto Kk | Recombinant DNA sequences coding for enzymes free from feedback inhibition, plasmids containing these sequences, transformed microorganisms useful for the production of aromatic amino acids, and their processes for the production by fermentation |
| DE4232468A1 (en) | 1992-09-28 | 1994-03-31 | Consortium Elektrochem Ind | Microorganisms for the production of tryptophan and process for their production |
| US5998178A (en) * | 1994-05-30 | 1999-12-07 | Ajinomoto Co., Ltd. | L-isoleucine-producing bacterium and method for preparing L-isoleucine through fermentation |
| US5639635A (en) | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
| JP3915275B2 (en) | 1998-10-16 | 2007-05-16 | 味の素株式会社 | Method for producing L-leucine |
| AU2223601A (en) * | 1999-12-24 | 2001-07-09 | Ajinomoto Co., Inc. | Process for producing l-amino acid and novel gene |
| RU2209246C2 (en) * | 2000-01-26 | 2003-07-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" | Small subunit of isozyme iii and isozyme iii of acetohydroxyacid synthetase from escherichia coli, dna fragment (variants), strain of bacterium escherichia coli as producer of l-valine (variants) and method for preparing l-valine |
| IT1320312B1 (en) * | 2000-04-21 | 2003-11-26 | Bitron Spa | DOOR-LOCKING DEVICE FOR HOUSEHOLD APPLIANCES. |
| RU2212447C2 (en) | 2000-04-26 | 2003-09-20 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" | Strain escherichia coli as producer of amino acid (variants) and method for preparing amino acid (variants) |
| JP4380029B2 (en) | 2000-07-05 | 2009-12-09 | 味の素株式会社 | Manufacturing method of substances using microorganisms |
| JP2002209596A (en) | 2001-01-19 | 2002-07-30 | Ajinomoto Co Inc | Method for producing l-amino acid |
| JP2002330763A (en) * | 2001-05-02 | 2002-11-19 | Ajinomoto Co Inc | Method for producing objective substance by fermentation |
| JP3932945B2 (en) | 2002-03-27 | 2007-06-20 | 味の素株式会社 | Method for producing L-amino acid |
-
2005
- 2005-04-25 US US11/113,270 patent/US7300776B2/en active Active
- 2005-04-26 CN CN2005800200952A patent/CN1969038B/en active Active
- 2005-04-26 DK DK05737046.2T patent/DK1740694T3/en active
- 2005-04-26 AT AT05737046T patent/ATE557096T1/en active
- 2005-04-26 BR BRPI0509982A patent/BRPI0509982B1/en active IP Right Grant
- 2005-04-26 EP EP05737046A patent/EP1740694B1/en active Active
- 2005-04-26 WO PCT/JP2005/008402 patent/WO2005103228A2/en active Application Filing
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