RU2002108173A - Targeted Gene Removal - Google Patents

Targeted Gene Removal

Info

Publication number
RU2002108173A
RU2002108173A RU2002108173/13A RU2002108173A RU2002108173A RU 2002108173 A RU2002108173 A RU 2002108173A RU 2002108173/13 A RU2002108173/13 A RU 2002108173/13A RU 2002108173 A RU2002108173 A RU 2002108173A RU 2002108173 A RU2002108173 A RU 2002108173A
Authority
RU
Russia
Prior art keywords
attp
plant
region
transgene
seq
Prior art date
Application number
RU2002108173/13A
Other languages
Russian (ru)
Inventor
Петер МЕЙЕР (GB)
Петер МЕЙЕР
Елена ЗУБКО (GB)
Елена ЗУБКО
Original Assignee
Юниверсити Оф Лидс (Gb)
Юниверсити Оф Лидс
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Юниверсити Оф Лидс (Gb), Юниверсити Оф Лидс filed Critical Юниверсити Оф Лидс (Gb)
Publication of RU2002108173A publication Critical patent/RU2002108173A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (21)

1. Способ удаления части трансгена после его интеграции в геном, заключающийся во фланкировании указанной части трансгена с каждой ее стороны областью присоединения Р (attP) бактериофага λ, причем область attP охватывает нуклеотидную последовательность, представленную SEQ ID NO:1, или ее фрагмент, который сохраняет ту же функцию, или нуклеиновые кислоты, которые гибридизуются в жестких условиях с ДНК SEQ ID NO:1 и функционируют как attP-область, или нуклеиновые кислоты, которые отличаются от ДНК SEQ ID NO:1 вследствие вырожденности генетического кода и которые функционируют как attP-область, и в индуцировании высокой частоты внутрихромосомной гомологичной рекомбинации между фланкирующими attP-областями, посредством чего удаляется указанная часть трансгена, помещенная между ними.1. The method of removing part of the transgene after its integration into the genome, which consists in flanking the specified part of the transgene on each side with the P (attP) attachment region of the bacteriophage λ, wherein the attP region covers the nucleotide sequence represented by SEQ ID NO: 1, or a fragment thereof retains the same function, either nucleic acids that hybridize under stringent conditions with SEQ ID NO: 1 DNA and function as an attP region, or nucleic acids that differ from SEQ ID NO: 1 DNA due to the degeneracy of the genetic code and which e function as attP-region, and a high frequency induction intrachromosomal homologous recombination between attP-flanking regions, whereby said removed portion transgene placed therebetween. 2. Способ по п.1, отличающийся тем, что указанный трансген включает маркерный ген, и/или векторную последовательность, и/или другую чужеродную вспомогательную нуклеиновую кислоту.2. The method according to claim 1, characterized in that said transgene comprises a marker gene and / or a vector sequence and / or other foreign auxiliary nucleic acid. 3. Способ по п.1 или 2, отличающийся тем, что маркерный ген сообщает устойчивость к антибиотикам и/или гербицидную устойчивость.3. The method according to claim 1 or 2, characterized in that the marker gene reports antibiotic resistance and / or herbicidal resistance. 4. Способ по любому из пп.1-3, отличающийся тем, что маркерный ген вызывает конкретные биосинтетические каскады реакций и/или устойчивость к воздействию окружающей среды.4. The method according to any one of claims 1 to 3, characterized in that the marker gene causes specific biosynthetic cascades of reactions and / or environmental resistance. 5. Способ по любому из пп.1-4, отличающийся тем, что маркерный ген выбирают из группы, состоящей из nptII, Ble, dhfr, cat, aphIV, SPT, аасС 3, аас С4, bar, EPSP, bxn, psbA, tfdA, DHPS, AK, sul, crsl-1 и tdc.5. The method according to any one of claims 1 to 4, characterized in that the marker gene is selected from the group consisting of nptII, Ble, dhfr, cat, aphIV, SPT, aacC 3, aac C4, bar, EPSP, bxn, psbA, tfdA, DHPS, AK, sul, crsl-1 and tdc. 6. Способ по любому из пп.1-5, отличающийся тем, что более чем одна часть, охватывающая маркерный ген, и/или векторную последовательность, и/или чужеродную нуклеиновую кислоту, удаляется из трансгена, и каждая такая часть, подлежащая удалению, фланкируется attP-областью.6. The method according to any one of claims 1 to 5, characterized in that more than one part encompassing the marker gene and / or vector sequence and / or foreign nucleic acid is removed from the transgene, and each such part to be removed, flanked by the attP region. 7. Способ по любому из пп.1-6, отличающийся тем, что область attP охватывает 352 пары оснований или ее функционально эквивалентный фрагмент, расположенный между положениями 27492 и 27844 бактериофага λ.7. The method according to any one of claims 1 to 6, characterized in that the attP region covers 352 base pairs or a functionally equivalent fragment thereof located between positions 27492 and 27844 of the bacteriophage λ. 8. Способ по любому из пп.1-7, отличающийся тем, что области attP находятся в кассете.8. The method according to any one of claims 1 to 7, characterized in that the attP regions are in the cassette. 9. Способ по п.8, отличающийся тем, что кассета дополнительно включает бустерную последовательность трансформации или ее фрагмент для усиления гомологичной и "незаконной" рекомбинации.9. The method of claim 8, wherein the cassette further includes a booster transformation sequence or fragment thereof to enhance homologous and “illegal” recombination. 10. Способ по п.8 или 9, отличающийся тем, что кассета включает эффекторный ген, такой как оризацистатин-I или его функциональный эквивалент.10. The method according to claim 8 or 9, characterized in that the cassette includes an effector gene, such as oryzacystatin-I or its functional equivalent. 11. Способ по любому из пп.1-10, отличающийся тем, что геном представляет собой растительный геном.11. The method according to any one of claims 1 to 10, characterized in that the genome is a plant genome. 12. Растение, или растительная клетка, или растительная ткань, полученные способом согласно любому из пп.1-11.12. A plant or plant cell or plant tissue obtained by the method according to any one of claims 1 to 11. 13. Способ, который представляет собой осуществление способа по п.11 для получения растения или создания растения, или растительной клетки, или растительной ткани по п.12 и, в любом случае выращивание растения и/или сбор его продуктов.13. The method, which is the implementation of the method according to claim 11 for producing a plant or creating a plant, or a plant cell, or plant tissue according to claim 12 and, in any case, growing a plant and / or collecting its products. 14. Растение, или растительная клетка, или растительная ткань, содержащие рекомбинантные attP-области.14. A plant, or plant cell, or plant tissue containing recombinant attP regions. 15. attP-кассета рекомбинации, содержащая маркерный ген и/или векторную последовательность и/или чужеродную вспомогательную нуклеиновую кислоту, фланкированные с каждой стороны attP-областью, представляющей собой нуклеотидную последовательность, изображенную SEQ ID NO:1, или ее фрагмент, который сохраняет ту же функцию, или нуклеиновые кислоты, которые гибридизуются в жестких условиях с ДНК SEQ ID NO:1 и функционируют как attP-область, или нуклеиновые кислоты, которые отличаются от ДНК SEQ ID NO:1 вследствие вырожденности генетического кода и которые функционируют как attP-область.15. attP-recombination cassette containing the marker gene and / or vector sequence and / or foreign auxiliary nucleic acid flanked on each side by the attP region, which is the nucleotide sequence depicted by SEQ ID NO: 1, or a fragment thereof which retains that the same function, or nucleic acids that hybridize under stringent conditions with the DNA of SEQ ID NO: 1 and function as an attP region, or nucleic acids that differ from the DNA of SEQ ID NO: 1 due to the degeneracy of the genetic code and which function BID as the attP-region. 16. Применение attP-кассеты рекомбинации по п.15 для удаления части, интегрированной в растительный геном.16. The use of the attP recombination cassette of claim 15 for removing a portion integrated into the plant genome. 17. Набор для удаления части трансгена после его интеграции в растительный геном, содержащий attP-кассету рекомбинации по п.15.17. A kit for removing a portion of a transgene after its integration into a plant genome containing the attP recombination cassette according to claim 15. 18. Растение, или растительная клетка, или растительная ткань, содержащие рекомбинантный трансген, интегрированный в их геном, характеризующиеся тем, что трансген ассоциирован с attP-областью бактериофага λ с их соответствующих сторон, причем attP-область представляет собой нуклеотидную последовательность, представленную SEQ ID NO:1, или ее фрагмент, который сохраняет ту же функцию, или нуклеиновые кислоты, которые гибридизуются в жестких условиях с ДНК SEQ ID NO:1 и функционируют как attP-область, или нуклеиновые кислоты, которые отличаются от ДНК SEQ ID NO:1 вследствие вырожденности генетического кода и которые функционируют как attP-область.18. A plant, or plant cell, or plant tissue containing a recombinant transgene integrated into their genome, characterized in that the transgene is associated with the attP region of the bacteriophage λ from their respective sides, wherein the attP region is the nucleotide sequence represented by SEQ ID NO: 1, or a fragment thereof that retains the same function, or nucleic acids that hybridize under stringent conditions with the DNA of SEQ ID NO: 1 and function as an attP region, or nucleic acids that differ from the DNA of SEQ ID NO: 1 due to the degeneracy of the genetic code and which function as an attP region. 19. Растение, или растительная клетка, или растительная ткань по п.18, отличающиеся тем, что они включают одну такую attP-областью бактериофага λ и один эффекторный трансген, интегрированный в их геном.19. The plant, or plant cell, or plant tissue according to claim 18, characterized in that they include one such attP region of the bacteriophage λ and one effector transgene integrated into their genome. 20. Растение, или растительная клетка, или растительная ткань по п.19, отличающиеся тем, что attP-область бактериофага λ и один трансген не ассоциированы с маркерным геном и/или векторной последовательностью и/или другой чужеродной вспомогательной нуклеиновой кислотой.20. The plant, or plant cell, or plant tissue according to claim 19, characterized in that the attP region of the bacteriophage λ and one transgene are not associated with a marker gene and / or vector sequence and / or other foreign auxiliary nucleic acid. 21. Растение, или растительная клетка, или растительная ткань по любому из пп.18-20, отличающиеся тем, что трансген дополнительно ассоциирован с бустерной последовательностью трансформации, или ее фрагментом, который способен усиливать гомологичную и "незаконную" рекомбинацию.21. A plant, or a plant cell, or plant tissue according to any one of claims 18 to 20, characterized in that the transgene is additionally associated with a booster transformation sequence, or a fragment thereof, which is capable of enhancing homologous and “illegal” recombination.
RU2002108173/13A 1999-09-17 2000-09-15 Targeted Gene Removal RU2002108173A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9921937.0A GB9921937D0 (en) 1999-09-17 1999-09-17 Targeted gens removal
GB9921937.0 1999-09-17

Publications (1)

Publication Number Publication Date
RU2002108173A true RU2002108173A (en) 2004-03-10

Family

ID=10861053

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2002108173/13A RU2002108173A (en) 1999-09-17 2000-09-15 Targeted Gene Removal

Country Status (10)

Country Link
EP (1) EP1212443A2 (en)
JP (1) JP2003510043A (en)
CN (1) CN1375010A (en)
AU (1) AU7532500A (en)
BR (1) BR0014521A (en)
CA (1) CA2384932A1 (en)
GB (1) GB9921937D0 (en)
HU (1) HUP0202805A2 (en)
RU (1) RU2002108173A (en)
WO (1) WO2001021780A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6750379B2 (en) 2000-03-09 2004-06-15 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
US6580019B1 (en) 2000-03-09 2003-06-17 Dekalb Genetics Corporation Non-reciprocal recombination-mediated transgene deletion in transgenic plants
WO2001081600A2 (en) * 2000-04-20 2001-11-01 Btg International Limited Transgenic plants
AU2006246975B2 (en) * 2005-05-17 2011-08-11 Ozgene Pty Ltd Sequential cloning system

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4673640A (en) * 1984-04-30 1987-06-16 Biotechnica International, Inc. Regulated protein production using site-specific recombination
NL8701450A (en) * 1987-06-22 1989-01-16 Solvay METHOD FOR TRANSFORMING CELLS.
JPH05507853A (en) * 1990-06-12 1993-11-11 ベイラー・カレッジ・オブ・メディシン Homologous recombination methods in animal and plant cells
US5736367A (en) * 1992-03-31 1998-04-07 Medimmune, Inc. Vectors and prokaryotes which autocatalytically delete antibiotic resistance
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
AU728915B2 (en) * 1996-05-09 2001-01-18 Nippon Paper Industries Co. Ltd. Vector for introducing a gene into a plant from which a selectable marker gene can be optionally removed
CN100342008C (en) * 1997-10-24 2007-10-10 茵维特罗根公司 Recombinational cloning using nucleic acids having recombinatin sites
WO2001007572A2 (en) * 1999-07-23 2001-02-01 The Regents Of The University Of California Dna recombination in eukaryotic cells by the bacteriophage phic31 recombination system

Also Published As

Publication number Publication date
WO2001021780A2 (en) 2001-03-29
JP2003510043A (en) 2003-03-18
EP1212443A2 (en) 2002-06-12
BR0014521A (en) 2002-06-11
CA2384932A1 (en) 2001-03-29
AU7532500A (en) 2001-04-24
GB9921937D0 (en) 1999-11-17
HUP0202805A2 (en) 2002-12-28
WO2001021780A3 (en) 2001-10-18
CN1375010A (en) 2002-10-16

Similar Documents

Publication Publication Date Title
CA2451517A1 (en) Defensin polynucleotides and methods of use
CA2388860A1 (en) Male-sterile brassica plants and methods for producing same
CA2318728A1 (en) Transcriptional regulatory region
IL193758A0 (en) Genetically engineered duckweed
TR200401186T4 (en) Artificial chromosome structures containing nucleic acid sequences capable of directing the formation of a recombinant RNA-virus.
CN106086031B (en) Pig flesh chalone gene editing site and its application
CN106754949B (en) Pig flesh chalone gene editing site 864-883 and its application
RU2002108173A (en) Targeted Gene Removal
RU2221044C2 (en) Dna segment able to confer to plant resistance against pest (variants), vector, method for preparing plants with reduced sensitivity to pest, method for plant protection against infection with pest, method for isolation dna segment, method for selective amplification of restriction fragments, oligonucleotide, diagnostic set, method for detection of dna segment, polypeptide
Chan et al. Mouse α-1-microglobulin/bikunin precursor: cDNA analysis, gene evolution and physical assignment of the gene next to the orosomucoid locus
BURT et al. The chicken transforming growth factor-β3 gene: genomic structure, transcriptional analysis, and chromosomal location
CA2045839A1 (en) Cloned n-methylhydantoinase
DE69836484D1 (en) ENDOGRAPHED RETROVIRAL SEQUENCES, RELATED TO AUTO-IMMUNE DISEASES OR WITH PREGNANCY DISORDERS
RU2005131582A (en) PLANT CELLS AND PLANT ORGANISMS WITH MODIFIED CELL GROWTH, DEVELOPMENT AND DIFFERENTIATION
NZ512865A (en) Nucleotide and polypeptide sequences encoding plant cell signaling proteins from Pinus radiata and Eucalyptus grandis
WO1997031012A1 (en) Gene specific universal mammalian sequence-tagged sites
CA2310726A1 (en) Plant seedling and embryo promoter
Zelnick et al. A composite transposon 3′ to the cow fetal globin gene binds a sequence specific factor
Reibel et al. An insulinoma nuclear factor binding to GGGCCC motifs in human insulin gene
Pata et al. The human ribosomal protein S6 gene: isolation, primary structure and location in chromosome 9
CA2487290A1 (en) Ehd1 gene promoting plant flowering and utilization thereof
CA2394792A1 (en) Promoter and intron from maize actin depolymerizing factor
FR2651007B1 (en) NUCLEIC ACID FRAGMENTS AND DIAGNOSTIC REAGENTS DERIVED FROM THE HHV6 / SIE GENOME AND METHOD FOR DIAGNOSING HHV6 INFECTIONS
CA2392845A1 (en) Maize mip synthase promoter
CN115141831A (en) Method for targeted knockout of bovine ZC3H10 gene based on CRISPR-Cas9 technology and application