WO2001021780A3 - Targeted gene removal - Google Patents

Targeted gene removal Download PDF

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Publication number
WO2001021780A3
WO2001021780A3 PCT/GB2000/003543 GB0003543W WO0121780A3 WO 2001021780 A3 WO2001021780 A3 WO 2001021780A3 GB 0003543 W GB0003543 W GB 0003543W WO 0121780 A3 WO0121780 A3 WO 0121780A3
Authority
WO
WIPO (PCT)
Prior art keywords
targeted gene
gene removal
transgene
attp
flanking
Prior art date
Application number
PCT/GB2000/003543
Other languages
French (fr)
Other versions
WO2001021780A2 (en
Inventor
Peter Meyer
Elena Zubko
Original Assignee
Univ Leeds
Peter Meyer
Elena Zubko
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leeds, Peter Meyer, Elena Zubko filed Critical Univ Leeds
Priority to AU75325/00A priority Critical patent/AU7532500A/en
Priority to BR0014521-1A priority patent/BR0014521A/en
Priority to HU0202805A priority patent/HUP0202805A2/en
Priority to JP2001525339A priority patent/JP2003510043A/en
Priority to EP00964384A priority patent/EP1212443A2/en
Priority to CA002384932A priority patent/CA2384932A1/en
Publication of WO2001021780A2 publication Critical patent/WO2001021780A2/en
Publication of WO2001021780A3 publication Critical patent/WO2001021780A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method of removing a part of a transgene after its integration into a genome comprising flanking the part of the transgene on each side with an attachment P region (attP) of bacteriophage μ and inducing intrachromasomal homologous recombination between the flanking attP regions so that the part of the transgene sandwiched between the attP regions is removed.
PCT/GB2000/003543 1999-09-17 2000-09-15 Targeted gene removal WO2001021780A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU75325/00A AU7532500A (en) 1999-09-17 2000-09-15 Targeted gene removal
BR0014521-1A BR0014521A (en) 1999-09-17 2000-09-15 Removal of targeted gene
HU0202805A HUP0202805A2 (en) 1999-09-17 2000-09-15 Targeted gene removal
JP2001525339A JP2003510043A (en) 1999-09-17 2000-09-15 Target gene removal
EP00964384A EP1212443A2 (en) 1999-09-17 2000-09-15 Targeted gene removal
CA002384932A CA2384932A1 (en) 1999-09-17 2000-09-15 Targeted gene removal

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9921937.0 1999-09-17
GBGB9921937.0A GB9921937D0 (en) 1999-09-17 1999-09-17 Targeted gens removal

Publications (2)

Publication Number Publication Date
WO2001021780A2 WO2001021780A2 (en) 2001-03-29
WO2001021780A3 true WO2001021780A3 (en) 2001-10-18

Family

ID=10861053

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/003543 WO2001021780A2 (en) 1999-09-17 2000-09-15 Targeted gene removal

Country Status (10)

Country Link
EP (1) EP1212443A2 (en)
JP (1) JP2003510043A (en)
CN (1) CN1375010A (en)
AU (1) AU7532500A (en)
BR (1) BR0014521A (en)
CA (1) CA2384932A1 (en)
GB (1) GB9921937D0 (en)
HU (1) HUP0202805A2 (en)
RU (1) RU2002108173A (en)
WO (1) WO2001021780A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6750379B2 (en) 2000-03-09 2004-06-15 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
US6580019B1 (en) 2000-03-09 2003-06-17 Dekalb Genetics Corporation Non-reciprocal recombination-mediated transgene deletion in transgenic plants
JP2003530888A (en) * 2000-04-20 2003-10-21 ビーティージー・インターナショナル・リミテッド Transgenic plants
US8202727B2 (en) * 2005-05-17 2012-06-19 Ozgene Pty Ltd. Sequential cloning system

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0160571A2 (en) * 1984-04-30 1985-11-06 Biotechnica International, Inc. Cloning vehicle
EP0299552A1 (en) * 1987-06-22 1989-01-18 Solvay A process for transforming cells
WO1991019796A1 (en) * 1990-06-12 1991-12-26 Baylor College Of Medicine Method for homologous recombination in animal and plant cells
WO1994017176A1 (en) * 1993-01-29 1994-08-04 Purdue Research Foundation Controlled modification of eukaryotic genomes
WO1996033269A1 (en) * 1995-04-20 1996-10-24 Medimmune, Inc. Vectors and prokaryotes which autocatalytically delete antibiotic resistance
EP0911412A1 (en) * 1996-05-09 1999-04-28 Nippon Paper Industries Co., Ltd. Vector for gene transfer into plant allowing optional deletion of marker gene
WO1999021977A1 (en) * 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
WO2001007572A2 (en) * 1999-07-23 2001-02-01 The Regents Of The University Of California Dna recombination in eukaryotic cells by the bacteriophage phic31 recombination system

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0160571A2 (en) * 1984-04-30 1985-11-06 Biotechnica International, Inc. Cloning vehicle
EP0299552A1 (en) * 1987-06-22 1989-01-18 Solvay A process for transforming cells
WO1991019796A1 (en) * 1990-06-12 1991-12-26 Baylor College Of Medicine Method for homologous recombination in animal and plant cells
WO1994017176A1 (en) * 1993-01-29 1994-08-04 Purdue Research Foundation Controlled modification of eukaryotic genomes
WO1996033269A1 (en) * 1995-04-20 1996-10-24 Medimmune, Inc. Vectors and prokaryotes which autocatalytically delete antibiotic resistance
EP0911412A1 (en) * 1996-05-09 1999-04-28 Nippon Paper Industries Co., Ltd. Vector for gene transfer into plant allowing optional deletion of marker gene
WO1999021977A1 (en) * 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
WO2001007572A2 (en) * 1999-07-23 2001-02-01 The Regents Of The University Of California Dna recombination in eukaryotic cells by the bacteriophage phic31 recombination system

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
D'ENFERT C: "Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5'-decarboxylase gene, pyrG, as a unique transformation marker", CURRENT GENETICS,US,NEW YORK, NY, vol. 30, no. 1, June 1996 (1996-06-01), pages 76 - 82, XP002107254, ISSN: 0172-8083 *
HORIE K ET AL: "A replacement vector used to introduce subtle mutations into mouse genes", GENE,NL,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, vol. 166, no. 2, 1995, pages 197 - 204, XP004043073, ISSN: 0378-1119 *
OW D W ET AL: "GENOME MANIPULATION THROUGH SITE-SPECIFIC RECOMBINATION", CRITICAL REVIEWS IN PLANT SCIENCES,US,CRC PRESS, BOCA RATON, FL, vol. 14, no. 3, 1995, pages 239 - 261, XP000614883, ISSN: 0735-2689 *
PEREDELCHUK M Y ET AL: "A method for construction of E. coli strains with multiple DNA insertions in the chromosome", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES,GB,ELSEVIER SCIENCE PUBLISHERS, BARKING, vol. 187, no. 2, 18 March 1997 (1997-03-18), pages 231 - 238, XP004093285, ISSN: 0378-1119 *
YODER J I ET AL: "TRANSFORMATION SYSTEMS FOR GENERATING MARKER-FREE TRANSGENIC PLANTS", BIO/TECHNOLOGY,US,NATURE PUBLISHING CO. NEW YORK, vol. 12, no. 3, 1 March 1994 (1994-03-01), pages 263 - 267, XP002043802, ISSN: 0733-222X *
ZUBKO ELENA ET AL: "Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes.", NATURE BIOTECHNOLOGY, vol. 18, no. 4, April 2000 (2000-04-01), pages 442 - 445, XP002165150, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
GB9921937D0 (en) 1999-11-17
AU7532500A (en) 2001-04-24
CA2384932A1 (en) 2001-03-29
EP1212443A2 (en) 2002-06-12
HUP0202805A2 (en) 2002-12-28
WO2001021780A2 (en) 2001-03-29
RU2002108173A (en) 2004-03-10
CN1375010A (en) 2002-10-16
BR0014521A (en) 2002-06-11
JP2003510043A (en) 2003-03-18

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