HUP0202805A2 - Targeted gene removal - Google Patents
Targeted gene removalInfo
- Publication number
- HUP0202805A2 HUP0202805A2 HU0202805A HUP0202805A HUP0202805A2 HU P0202805 A2 HUP0202805 A2 HU P0202805A2 HU 0202805 A HU0202805 A HU 0202805A HU P0202805 A HUP0202805 A HU P0202805A HU P0202805 A2 HUP0202805 A2 HU P0202805A2
- Authority
- HU
- Hungary
- Prior art keywords
- plant
- targeted gene
- gene removal
- removal
- genes
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 238000000034 method Methods 0.000 abstract 2
- 239000003550 marker Substances 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 238000003976 plant breeding Methods 0.000 abstract 1
- 238000010188 recombinant method Methods 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A találmány tárgyát egy, szelektálható marker gén vagy gének vagyidegen kisegítő nukleinsavak növényből, közelebbről transzgénikusnövényből történő eltávolítására szolgáló rekombináns eljárás képezi,továbbá a találmány szerinti eljárás megvalósításához szükségesvektorok és a találmány szerinti eljárás termékei. A találmányszerinti megoldás a növénytermesztésben és növénynemesítésbenalkalmazható. ÓThe subject of the invention is a recombinant method for the removal of selectable marker gene or genes or foreign auxiliary nucleic acids from a plant, more precisely from a transgenic plant, as well as the vectors necessary for the implementation of the method according to the invention and the products of the method according to the invention. The solution according to the invention can be used in plant cultivation and plant breeding. HE
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9921937.0A GB9921937D0 (en) | 1999-09-17 | 1999-09-17 | Targeted gens removal |
PCT/GB2000/003543 WO2001021780A2 (en) | 1999-09-17 | 2000-09-15 | Targeted gene removal |
Publications (1)
Publication Number | Publication Date |
---|---|
HUP0202805A2 true HUP0202805A2 (en) | 2002-12-28 |
Family
ID=10861053
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HU0202805A HUP0202805A2 (en) | 1999-09-17 | 2000-09-15 | Targeted gene removal |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP1212443A2 (en) |
JP (1) | JP2003510043A (en) |
CN (1) | CN1375010A (en) |
AU (1) | AU7532500A (en) |
BR (1) | BR0014521A (en) |
CA (1) | CA2384932A1 (en) |
GB (1) | GB9921937D0 (en) |
HU (1) | HUP0202805A2 (en) |
RU (1) | RU2002108173A (en) |
WO (1) | WO2001021780A2 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6750379B2 (en) | 2000-03-09 | 2004-06-15 | Dekalb Genetics Corporation | Homologous recombination-mediated transgene alterations in plants |
US6580019B1 (en) | 2000-03-09 | 2003-06-17 | Dekalb Genetics Corporation | Non-reciprocal recombination-mediated transgene deletion in transgenic plants |
JP2003530888A (en) * | 2000-04-20 | 2003-10-21 | ビーティージー・インターナショナル・リミテッド | Transgenic plants |
US8202727B2 (en) * | 2005-05-17 | 2012-06-19 | Ozgene Pty Ltd. | Sequential cloning system |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4673640A (en) * | 1984-04-30 | 1987-06-16 | Biotechnica International, Inc. | Regulated protein production using site-specific recombination |
NL8701450A (en) * | 1987-06-22 | 1989-01-16 | Solvay | METHOD FOR TRANSFORMING CELLS. |
EP0535144A4 (en) * | 1990-06-12 | 1993-08-11 | Baylor College Of Medicine | Method for homologous recombination in animal and plant cells |
US5736367A (en) * | 1992-03-31 | 1998-04-07 | Medimmune, Inc. | Vectors and prokaryotes which autocatalytically delete antibiotic resistance |
US5527695A (en) * | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
NZ333259A (en) * | 1996-05-09 | 2001-02-23 | Nippon Paper Ind Co | Vector for introducing desired gene with selectable marker which allows the desired gene to be removed by removal of the selectable marker (anchor selectable marker gene) |
WO1999021977A1 (en) * | 1997-10-24 | 1999-05-06 | Life Technologies, Inc. | Recombinational cloning using nucleic acids having recombination sites |
JP4808880B2 (en) * | 1999-07-23 | 2011-11-02 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | DNA recombination in eukaryotic cells by bacteriophage PHIC31 recombination system |
-
1999
- 1999-09-17 GB GBGB9921937.0A patent/GB9921937D0/en not_active Ceased
-
2000
- 2000-09-15 RU RU2002108173/13A patent/RU2002108173A/en unknown
- 2000-09-15 BR BR0014521-1A patent/BR0014521A/en not_active Application Discontinuation
- 2000-09-15 WO PCT/GB2000/003543 patent/WO2001021780A2/en not_active Application Discontinuation
- 2000-09-15 HU HU0202805A patent/HUP0202805A2/en unknown
- 2000-09-15 JP JP2001525339A patent/JP2003510043A/en active Pending
- 2000-09-15 EP EP00964384A patent/EP1212443A2/en not_active Withdrawn
- 2000-09-15 CN CN00813040.XA patent/CN1375010A/en active Pending
- 2000-09-15 CA CA002384932A patent/CA2384932A1/en not_active Abandoned
- 2000-09-15 AU AU75325/00A patent/AU7532500A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
GB9921937D0 (en) | 1999-11-17 |
AU7532500A (en) | 2001-04-24 |
CA2384932A1 (en) | 2001-03-29 |
EP1212443A2 (en) | 2002-06-12 |
WO2001021780A3 (en) | 2001-10-18 |
WO2001021780A2 (en) | 2001-03-29 |
RU2002108173A (en) | 2004-03-10 |
CN1375010A (en) | 2002-10-16 |
BR0014521A (en) | 2002-06-11 |
JP2003510043A (en) | 2003-03-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE330007T1 (en) | METHOD FOR INCREASE THE EXPRESSION OF ENDOGENE OR EXOGENEOUS GENE PRODUCTS IN PLANTS | |
ATE494370T1 (en) | TRANSPOSON-BASED VECTORS AND METHOD FOR INTEGRATION OF NUCLEIC ACIDS | |
HUP0002578A2 (en) | Male tissue-preferred regulatory region and method of using same | |
DE69939744D1 (en) | PROCESS FOR PRODUCING STRESS-STERLING PLANTS | |
DE69430993T2 (en) | CONTROLLED EXPRESSION OF TRANSGENIC CONSTRUCTS IN PLANT PLASTIDES | |
DE69333529D1 (en) | PLANT VIRUS VECTOR, PLASMID, METHOD OF EXPRESSION OF FOREIGN GENES AND METHOD FOR OBTAINING THE ABOVE GENE PRODUCTS | |
DE60236182D1 (en) | ACCIDENTAL INTEGRATION OF POLYNUCLEOTIDE AFTER IN VIVO LINEARIZATION | |
ATE323163T1 (en) | WHEAT RHT GENE FOR GENETIC CONTROL OF PLANT GROWTH AND DEVELOPMENT | |
Kawakami et al. | Excision of the Tol2 transposable element of the medaka fish Oryzias latipes in Xenopus laevis and Xenopus tropicalis | |
HUP0202608A2 (en) | Seed-preferred promoter from maize | |
WO2000017365A3 (en) | Binary viral expression system in plants | |
HUP0002649A2 (en) | Induction of male sterility in plants by expression of high levels of avidin | |
RU94046200A (en) | Dna sequences, derivatives of dna sequences, plasmids, method of sequences using, method of plasmid using, transgenic plants | |
DE60024971D1 (en) | DNA ENGINEERING FOR VEGETABLE LIPASE, TRANSGENIC PLANTS AND A METHOD FOR CONTROLLING SENESCENCE IN PLANTS | |
DE60332107D1 (en) | SEED-SPECIFIC USP PROMOTERS FOR THE EXPRESSION OF GENES IN PLANTS | |
WO2001070777A3 (en) | Leafy cotyledon2 genes and their uses | |
ATE387497T1 (en) | SEED-SPECIFIC 7S ALPHA PROMOTERS FOR EXPRESSING GENES IN PLANTS | |
HUP0202805A2 (en) | Targeted gene removal | |
ATE338814T1 (en) | METHOD FOR CULTIVATION OF CELLS AND PROPAGATION OF VIRUSES | |
DE60035892D1 (en) | GENES AND METHOD FOR GROWTH MANIPULATION | |
DE60024658D1 (en) | PROMOTER FOR REGULATING FOREIGN GENE EXPRESSION | |
DE60138972D1 (en) | Transgenic plants with the gene of the neoxanthine-cleaving enzyme | |
HUP0202852A2 (en) | Modified ubiquitin regulatory system | |
ITMI20030860A1 (en) | METHOD FOR SELECTIVE INHIBITION OF THE N-MYC GENE | |
ATE445702T1 (en) | BUTINOL I ESTERASE |