PT2964784T - Sistema de deteção de reação em cadeia da polimerase - Google Patents

Sistema de deteção de reação em cadeia da polimerase

Info

Publication number
PT2964784T
PT2964784T PT147171672T PT14717167T PT2964784T PT 2964784 T PT2964784 T PT 2964784T PT 147171672 T PT147171672 T PT 147171672T PT 14717167 T PT14717167 T PT 14717167T PT 2964784 T PT2964784 T PT 2964784T
Authority
PT
Portugal
Prior art keywords
detection system
chain reaction
polymerase chain
reaction detection
polymerase
Prior art date
Application number
PT147171672T
Other languages
English (en)
Original Assignee
Lgc Genomics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lgc Genomics Ltd filed Critical Lgc Genomics Ltd
Publication of PT2964784T publication Critical patent/PT2964784T/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/107Temperature of melting, i.e. Tm
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00Reactions characterised by the features used to influence the efficiency or specificity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10Detection mode being characterised by the assay principle
    • C12Q2565/101Interaction between at least two labels

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
PT147171672T 2013-03-06 2014-03-05 Sistema de deteção de reação em cadeia da polimerase PT2964784T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1304030.8A GB2515990B (en) 2013-03-06 2013-03-06 Polymerase chain reaction detection system

Publications (1)

Publication Number Publication Date
PT2964784T true PT2964784T (pt) 2020-06-17

Family

ID=48142528

Family Applications (1)

Application Number Title Priority Date Filing Date
PT147171672T PT2964784T (pt) 2013-03-06 2014-03-05 Sistema de deteção de reação em cadeia da polimerase

Country Status (25)

Country Link
US (1) US10155975B2 (pt)
EP (1) EP2964784B1 (pt)
JP (1) JP6636335B2 (pt)
CN (2) CN116622816A (pt)
AU (1) AU2014224378B2 (pt)
BR (1) BR112015021615B8 (pt)
CA (1) CA2902920C (pt)
CL (1) CL2015002475A1 (pt)
CY (1) CY1123180T1 (pt)
DK (1) DK2964784T3 (pt)
ES (1) ES2795679T3 (pt)
GB (1) GB2515990B (pt)
HK (1) HK1216110A1 (pt)
HR (1) HRP20201117T1 (pt)
HU (1) HUE051443T2 (pt)
LT (1) LT2964784T (pt)
MY (1) MY182547A (pt)
NZ (1) NZ711109A (pt)
PL (1) PL2964784T3 (pt)
PT (1) PT2964784T (pt)
RS (1) RS60515B1 (pt)
SG (1) SG11201506864XA (pt)
SI (1) SI2964784T1 (pt)
WO (1) WO2014135872A1 (pt)
ZA (1) ZA201505950B (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201511156D0 (en) 2015-06-24 2015-08-05 Glaxosmithkline Australia Pty Ltd And University Of York Production of alkaloids
KR101899371B1 (ko) 2017-07-25 2018-10-29 (주)엔바이오텍 핵산 복합체 페어, 핵산 복합체 페어를 포함하는 pcr용 키트, 및 핵산 복합체 페어를 이용한 타겟 검출 방법
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
CN108715888A (zh) * 2018-06-07 2018-10-30 王卫东 一种基于fret的pcr均相检测系统及其应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
CA2176193A1 (en) * 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
EP1666609B1 (en) 1997-02-28 2012-09-26 Quest Diagnostics Investments Incorporated Fluorescence energy transfer by competitive hybridization
WO1999049293A2 (en) 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US20100105050A1 (en) * 2000-09-07 2010-04-29 Transnetyx, Inc. Real time detection of genetic sequences using a bipartite probe
AU2004275903B2 (en) * 2003-09-29 2012-04-12 Topigen Pharmaceutique Inc. Oligonucleotide compositions and methods for treating disease including inflammatory conditions
CA2607369A1 (en) * 2005-05-02 2006-11-09 Stratagene California Oligonucleotide probe/primer compositions and methods for polynucleotide detection
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
WO2009126678A2 (en) * 2008-04-08 2009-10-15 Cornell University Detection of target nucleic acid sequences using fluorescence resonance energy transfer
EP3269811B1 (en) * 2010-07-28 2020-10-07 Advanced Innovation Development Co. Ltd. Nucleic acid having adjuvant activity and use thereof
CN108342453A (zh) * 2011-05-09 2018-07-31 富鲁达公司 基于探针的核酸检测
PT2828399T (pt) * 2012-03-22 2017-04-04 Lgc Genomics Ltd Sistema de deteção de reação em cadeia de polimerase utilizando oligonucleótidos compreendendo um grupo fosforotioato

Also Published As

Publication number Publication date
AU2014224378B2 (en) 2018-10-25
HRP20201117T1 (hr) 2020-10-30
JP6636335B2 (ja) 2020-01-29
GB2515990B (en) 2016-05-04
ES2795679T3 (es) 2020-11-24
SI2964784T1 (sl) 2020-08-31
HK1216110A1 (zh) 2016-10-14
ZA201505950B (en) 2023-06-28
WO2014135872A1 (en) 2014-09-12
BR112015021615B1 (pt) 2022-08-02
CL2015002475A1 (es) 2016-05-06
GB201304030D0 (en) 2013-04-17
MY182547A (en) 2021-01-25
LT2964784T (lt) 2020-06-10
BR112015021615A2 (pt) 2018-03-06
BR112015021615B8 (pt) 2022-10-25
CY1123180T1 (el) 2021-10-29
AU2014224378A1 (en) 2015-09-03
CA2902920C (en) 2019-07-30
RS60515B1 (sr) 2020-08-31
CA2902920A1 (en) 2014-09-12
US20160002712A1 (en) 2016-01-07
CN105051212A (zh) 2015-11-11
PL2964784T3 (pl) 2020-10-19
HUE051443T2 (hu) 2021-03-01
JP2016513451A (ja) 2016-05-16
US10155975B2 (en) 2018-12-18
EP2964784B1 (en) 2020-04-22
GB2515990A (en) 2015-01-14
DK2964784T3 (da) 2020-06-15
SG11201506864XA (en) 2015-09-29
CN116622816A (zh) 2023-08-22
EP2964784A1 (en) 2016-01-13
NZ711109A (en) 2019-11-29

Similar Documents

Publication Publication Date Title
SG10201710049RA (en) Compositions, methods and systems for polymerase chain reaction assays
HK1216655A1 (zh) 用於 甲基化分析的引物
GB201306444D0 (en) Single nucleotide detection method
GB201416860D0 (en) Low-impact crash detection system
GB201306445D0 (en) Single nucleotide detection method
GB201409951D0 (en) Object detection system
GB2526520B (en) Biochemical reaction system
EP2971147A4 (en) MULTIPLEX ALLELNACHWEIS
EP2997534A4 (en) ROUTINE PROPOSED SYSTEM
HUE042608T2 (hu) Érzékelõ rendszer
GB201409953D0 (en) Object detection system
HK1216110A1 (zh) 聚合酶鏈式反應檢測系統
GB201203938D0 (en) Assay system
GB201307843D0 (en) Haplotype detection
IL245222A0 (en) A method for discovering DNA
ZA201602741B (en) Fire detecting system
SG2013055744A (en) Improved detection system
GB2527216B (en) Temperature detection system
GB201318948D0 (en) Detection system
GB201313507D0 (en) Detection system
GB201311357D0 (en) Detection System
GB201306457D0 (en) Detection system
GB201303069D0 (en) Detection system
GB201307844D0 (en) Detection system
GB202107480D0 (en) Biochemical reaction system