BR112015021615A2 - sistema de detecção de reação em cadeia da polimerase - Google Patents

sistema de detecção de reação em cadeia da polimerase

Info

Publication number
BR112015021615A2
BR112015021615A2 BR112015021615-3A BR112015021615A BR112015021615A2 BR 112015021615 A2 BR112015021615 A2 BR 112015021615A2 BR 112015021615 A BR112015021615 A BR 112015021615A BR 112015021615 A2 BR112015021615 A2 BR 112015021615A2
Authority
BR
Brazil
Prior art keywords
chain reaction
detection system
polymerase chain
reaction detection
detection
Prior art date
Application number
BR112015021615-3A
Other languages
English (en)
Other versions
BR112015021615B1 (pt
BR112015021615B8 (pt
Inventor
Jain Nisha
Edmond Holme John
Original Assignee
Lgc Genomics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lgc Genomics Ltd filed Critical Lgc Genomics Ltd
Publication of BR112015021615A2 publication Critical patent/BR112015021615A2/pt
Publication of BR112015021615B1 publication Critical patent/BR112015021615B1/pt
Publication of BR112015021615B8 publication Critical patent/BR112015021615B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Abstract

resumo da patente de invenã§ã£o para: “sistema de detecã‡ãƒo de reaã‡ãƒo em cadeia da polimerase”. a presente invenã§ã£o se refere a mã©todos e kits para a detecã§ã£o de ã¡cido nucleico em um sistema de ensaio.
BR112015021615A 2013-03-06 2014-03-05 Sistema de detecção de reação em cadeia da polimerase BR112015021615B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1304030.8A GB2515990B (en) 2013-03-06 2013-03-06 Polymerase chain reaction detection system
GB1304030.8 2013-03-06
PCT/GB2014/050650 WO2014135872A1 (en) 2013-03-06 2014-03-05 Polymerase chain reaction detection system

Publications (3)

Publication Number Publication Date
BR112015021615A2 true BR112015021615A2 (pt) 2018-03-06
BR112015021615B1 BR112015021615B1 (pt) 2022-08-02
BR112015021615B8 BR112015021615B8 (pt) 2022-10-25

Family

ID=48142528

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015021615A BR112015021615B8 (pt) 2013-03-06 2014-03-05 Sistema de detecção de reação em cadeia da polimerase

Country Status (25)

Country Link
US (1) US10155975B2 (pt)
EP (1) EP2964784B1 (pt)
JP (1) JP6636335B2 (pt)
CN (2) CN116622816A (pt)
AU (1) AU2014224378B2 (pt)
BR (1) BR112015021615B8 (pt)
CA (1) CA2902920C (pt)
CL (1) CL2015002475A1 (pt)
CY (1) CY1123180T1 (pt)
DK (1) DK2964784T3 (pt)
ES (1) ES2795679T3 (pt)
GB (1) GB2515990B (pt)
HK (1) HK1216110A1 (pt)
HR (1) HRP20201117T1 (pt)
HU (1) HUE051443T2 (pt)
LT (1) LT2964784T (pt)
MY (1) MY182547A (pt)
NZ (1) NZ711109A (pt)
PL (1) PL2964784T3 (pt)
PT (1) PT2964784T (pt)
RS (1) RS60515B1 (pt)
SG (1) SG11201506864XA (pt)
SI (1) SI2964784T1 (pt)
WO (1) WO2014135872A1 (pt)
ZA (1) ZA201505950B (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201511156D0 (en) 2015-06-24 2015-08-05 Glaxosmithkline Australia Pty Ltd And University Of York Production of alkaloids
KR101899371B1 (ko) * 2017-07-25 2018-10-29 (주)엔바이오텍 핵산 복합체 페어, 핵산 복합체 페어를 포함하는 pcr용 키트, 및 핵산 복합체 페어를 이용한 타겟 검출 방법
GB201713251D0 (en) 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
CN108715888A (zh) * 2018-06-07 2018-10-30 王卫东 一种基于fret的pcr均相检测系统及其应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
CA2176193A1 (en) * 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
EP1666609B1 (en) 1997-02-28 2012-09-26 Quest Diagnostics Investments Incorporated Fluorescence energy transfer by competitive hybridization
WO1999049293A2 (en) * 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US20100105050A1 (en) * 2000-09-07 2010-04-29 Transnetyx, Inc. Real time detection of genetic sequences using a bipartite probe
EP2314595A3 (en) * 2003-09-29 2011-07-20 Topigen Pharmaceuticals Inc. Oligonucleotide compositions and methods for treating disease including inflammatory conditions
JP2008539730A (ja) * 2005-05-02 2008-11-20 ストラタジーン カリフォルニア オリゴヌクレオチドのプローブ／プライマー組成物、及びポリヌクレオチドの検出方法
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
US20110136116A1 (en) * 2008-04-08 2011-06-09 Cornell University Detection of target nucleic acid sequences using fluorescence resonance energy transfer
JP5871212B2 (ja) * 2010-07-28 2016-03-01 国立大学法人北海道大学 アジュバント活性を有する新規核酸およびその利用
CN103635594B (zh) * 2011-05-09 2018-02-13 富鲁达公司 基于探针的核酸检测
SG11201502154UA (en) 2012-03-22 2015-05-28 Lgc Genomics Ltd Polymerase chain reaction detection system using oligonucleotides comprising a phosphorothioate group

Also Published As

Publication number Publication date
ZA201505950B (en) 2023-06-28
EP2964784A1 (en) 2016-01-13
AU2014224378A1 (en) 2015-09-03
GB2515990A (en) 2015-01-14
WO2014135872A1 (en) 2014-09-12
SG11201506864XA (en) 2015-09-29
US10155975B2 (en) 2018-12-18
CA2902920A1 (en) 2014-09-12
GB201304030D0 (en) 2013-04-17
EP2964784B1 (en) 2020-04-22
PL2964784T3 (pl) 2020-10-19
CA2902920C (en) 2019-07-30
RS60515B1 (sr) 2020-08-31
PT2964784T (pt) 2020-06-17
MY182547A (en) 2021-01-25
CN116622816A (zh) 2023-08-22
HRP20201117T1 (hr) 2020-10-30
ES2795679T3 (es) 2020-11-24
CL2015002475A1 (es) 2016-05-06
LT2964784T (lt) 2020-06-10
SI2964784T1 (sl) 2020-08-31
GB2515990B (en) 2016-05-04
US20160002712A1 (en) 2016-01-07
JP6636335B2 (ja) 2020-01-29
CY1123180T1 (el) 2021-10-29
NZ711109A (en) 2019-11-29
JP2016513451A (ja) 2016-05-16
HK1216110A1 (zh) 2016-10-14
AU2014224378B2 (en) 2018-10-25
BR112015021615B1 (pt) 2022-08-02
CN105051212A (zh) 2015-11-11
HUE051443T2 (hu) 2021-03-01
DK2964784T3 (da) 2020-06-15
BR112015021615B8 (pt) 2022-10-25

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 05/03/2014, OBSERVADAS AS CONDICOES LEGAIS

B09W Correction of the decision to grant [chapter 9.1.4 patent gazette]

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B16C Correction of notification of the grant [chapter 16.3 patent gazette]

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