PT116915A - Método para a extração de colagénio purificado de matrizes biológicas - Google Patents
Método para a extração de colagénio purificado de matrizes biológicasInfo
- Publication number
- PT116915A PT116915A PT116915A PT11691520A PT116915A PT 116915 A PT116915 A PT 116915A PT 116915 A PT116915 A PT 116915A PT 11691520 A PT11691520 A PT 11691520A PT 116915 A PT116915 A PT 116915A
- Authority
- PT
- Portugal
- Prior art keywords
- collagen
- removal
- extraction
- biomass
- preparation
- Prior art date
Links
- 102000008186 Collagen Human genes 0.000 title abstract 6
- 108010035532 Collagen Proteins 0.000 title abstract 6
- 229920001436 collagen Polymers 0.000 title abstract 6
- 238000000605 extraction Methods 0.000 title abstract 2
- 239000002028 Biomass Substances 0.000 abstract 2
- 102000012422 Collagen Type I Human genes 0.000 abstract 2
- 108010022452 Collagen Type I Proteins 0.000 abstract 2
- 239000011159 matrix material Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000002360 preparation method Methods 0.000 abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 239000012670 alkaline solution Substances 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 229940096422 collagen type i Drugs 0.000 abstract 1
- 239000000356 contaminant Substances 0.000 abstract 1
- 239000002537 cosmetic Substances 0.000 abstract 1
- 238000000502 dialysis Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000000227 grinding Methods 0.000 abstract 1
- 238000010297 mechanical methods and process Methods 0.000 abstract 1
- 230000005226 mechanical processes and functions Effects 0.000 abstract 1
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 238000000956 solid--liquid extraction Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Peptides Or Proteins (AREA)
Abstract
A PRESENTE INVENÇÃO DESCREVE UM MÉTODO PARA A OBTENÇÃO DE COLAGÉNIO TIPO I DE MATRIZES BIOLÓGICAS. ESTE MÉTODO COMPREENDE ESSENCIALMENTE TRÊS ETAPAS PRINCIPAIS: PREPARAÇÃO E REMOÇÃO DE CONTAMINANTES DA BIOMASSA; EXTRAÇÃO DO COLAGÉNIO; E RECUPERAÇÃO DO COLAGÉNIO PURIFICADO NA SUA FORMA SÓLIDA. A PRIMEIRA ETAPA DESCREVE A PREPARAÇÃO DE BIOMASSA COM A SUA MOAGEM POR PROCESSO MECÂNICO, REMOÇÃO DAS PROTEÍNAS NÃO-COLAGÉNICAS E REMOÇÃO DE GORDURAS RECORRENDO AO USO DE UMA SOLUÇÃO ALCALINA E UMA SOLUÇÃO ALCOÓLICA, RESPETIVAMENTE, PARA OBTENÇÃO DE UMA MATRIZ MAIS PURA EM COLAGÉNIO TIPO I. A SEGUNDA ETAPA COMPREENDE A EXTRAÇÃO SÓLIDO-LÍQUIDO DO TEOR DO COLAGÉNIO DA MATRIZ COM O USO DE UMA SOLUÇÃO AQUOSA DE UM SOLVENTE EUTÉTICO PROFUNDO DE PH ÁCIDO. POR FIM, O TEOR DE COLAGÉNIO É RECUPERADO COM A AÇÃO DE UM AGENTE PRECIPITANTE, DIÁLISE E SECAGEM. O COLAGÉNIO OBTIDO COM APLICAÇÃO NAS ÁREAS ALIMENTAR, FARMACÊUTICA, MÉDICA, COSMÉTICA, BIOMÉDICAE NA ENGENHARIA DE TECIDOS.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PT116915A PT116915B (pt) | 2020-12-03 | 2020-12-03 | Método para a extração de colagénio purificado de matrizes biológicas |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PT116915A PT116915B (pt) | 2020-12-03 | 2020-12-03 | Método para a extração de colagénio purificado de matrizes biológicas |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PT116915A true PT116915A (pt) | 2022-06-03 |
| PT116915B PT116915B (pt) | 2023-05-05 |
Family
ID=82399692
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT116915A PT116915B (pt) | 2020-12-03 | 2020-12-03 | Método para a extração de colagénio purificado de matrizes biológicas |
Country Status (1)
| Country | Link |
|---|---|
| PT (1) | PT116915B (pt) |
-
2020
- 2020-12-03 PT PT116915A patent/PT116915B/pt active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| PT116915B (pt) | 2023-05-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| BB1A | Laying open of patent application |
Effective date: 20220421 |
|
| FG3A | Patent granted, date of granting |
Effective date: 20230502 |