PL442126A1 - Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley that was in contact with zinc and cadmium - Google Patents

Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley that was in contact with zinc and cadmium

Info

Publication number
PL442126A1
PL442126A1 PL442126A PL44212622A PL442126A1 PL 442126 A1 PL442126 A1 PL 442126A1 PL 442126 A PL442126 A PL 442126A PL 44212622 A PL44212622 A PL 44212622A PL 442126 A1 PL442126 A1 PL 442126A1
Authority
PL
Poland
Prior art keywords
barley
level
gene expression
identifying
cadmium
Prior art date
Application number
PL442126A
Other languages
Polish (pl)
Inventor
Marek Marzec
Original Assignee
Uniwersytet Śląski W Katowicach
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Śląski W Katowicach filed Critical Uniwersytet Śląski W Katowicach
Priority to PL442126A priority Critical patent/PL442126A1/en
Publication of PL442126A1 publication Critical patent/PL442126A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia są sekwencje starterów pozwalające na analizę poziomu ekspresji genów w korzeniach jęczmienia oraz sposób identyfikacji jęczmienia, który miał kontakt z cynkiem i kadmem, w oparciu o analizę poziomów ekspresji tych genów. Bardziej szczegółowo zgłoszenie dotyczy starterów do identyfikacji genów o identyfikatorach HORVU.MOREX.r2.6HG05214100, HORVU.MOREX.r2.5HG0406420, kodujących odpowiednio reduktazę koenzymu A oraz białko wiążące wapń. Dla każdego z genów zaprojektowano startery pozwalające na określenie ich poziomu ekspresji w korzeniach jęczmienia z użyciem zaproponowanego systemu badania poziomu ekspresji genów.The subject of the application are primer sequences enabling the analysis of the level of gene expression in barley roots and a method of identifying barley that has been in contact with zinc and cadmium, based on the analysis of the expression levels of these genes. In more detail, the application concerns primers for identifying genes with the identifiers HORVU.MOREX.r2.6HG05214100, HORVU.MOREX.r2.5HG0406420, encoding coenzyme A reductase and calcium-binding protein, respectively. Primers were designed for each gene to determine their expression level in barley roots using the proposed gene expression level testing system.

PL442126A 2022-08-27 2022-08-27 Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley that was in contact with zinc and cadmium PL442126A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL442126A PL442126A1 (en) 2022-08-27 2022-08-27 Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley that was in contact with zinc and cadmium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL442126A PL442126A1 (en) 2022-08-27 2022-08-27 Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley that was in contact with zinc and cadmium

Publications (1)

Publication Number Publication Date
PL442126A1 true PL442126A1 (en) 2024-03-04

Family

ID=90106974

Family Applications (1)

Application Number Title Priority Date Filing Date
PL442126A PL442126A1 (en) 2022-08-27 2022-08-27 Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley that was in contact with zinc and cadmium

Country Status (1)

Country Link
PL (1) PL442126A1 (en)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DERAKHSHANI B., JAFARY H., MALEKI ZANJANI B., HASANPUR K., MISHINA K., TANAKA T. I IN..: "PLoS ONE 15(4): e0230820", COMBINED QTL MAPPING AND RNA-SEQ PROFILING REVEALS CANDIDATE GENES ASSOCIATED WITH CADMIUM TOLERANCE IN BARLEY, DOI: doi.org/10.1371/journal.pone.0230820 *
JINGWEN TIONG, GLENN MCDONALD, YUSUF GENC, NEIL SHIRLEY, PETER LANGRIDGE, CHUN Y. HUANG,: "New Phytologist (2015) 207:1097–1109", INCREASED EXPRESSION OF SIX ZIP FAMILY GENES BY ZINC (ZN)DEFICIENCY IS ASSOCIATED WITH ENHANCED UPTAKE AND ROOT-TO-SHOOT TRANSLOCATION OF ZN IN BARLEY (HORDEUM VULGARE), DOI: 10.1111/nph.13413 *
KINTLOVÁ M., VRÁNA J., HOBZA R., BLAVET N., HUDZIECZEK V.: "Front. Plant Sci. 12: 629089.", TRANSCRIPTOME RESPONSE TO CADMIUM EXPOSURE IN BARLEY (HORDEUM VULGARE L.), DOI: 10.3389/fpls.2021.629089 *

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