PL442115A1 - Primer sequences enabling analysis of gene expression levels in barley roots and method of identifying barley genotypes with reduced tolerance to zinc and cadmium - Google Patents
Primer sequences enabling analysis of gene expression levels in barley roots and method of identifying barley genotypes with reduced tolerance to zinc and cadmiumInfo
- Publication number
- PL442115A1 PL442115A1 PL442115A PL44211522A PL442115A1 PL 442115 A1 PL442115 A1 PL 442115A1 PL 442115 A PL442115 A PL 442115A PL 44211522 A PL44211522 A PL 44211522A PL 442115 A1 PL442115 A1 PL 442115A1
- Authority
- PL
- Poland
- Prior art keywords
- barley
- zinc
- cadmium
- gene expression
- identifying
- Prior art date
Links
- 241000209219 Hordeum Species 0.000 title abstract 5
- 235000007340 Hordeum vulgare Nutrition 0.000 title abstract 5
- 230000014509 gene expression Effects 0.000 title abstract 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 title abstract 3
- 229910052793 cadmium Inorganic materials 0.000 title abstract 3
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 title abstract 3
- 229910052725 zinc Inorganic materials 0.000 title abstract 3
- 239000011701 zinc Substances 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 241000196324 Embryophyta Species 0.000 abstract 1
- 102000003992 Peroxidases Human genes 0.000 abstract 1
- 102000019229 U-box domains Human genes 0.000 abstract 1
- 108050006631 U-box domains Proteins 0.000 abstract 1
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 229910001385 heavy metal Inorganic materials 0.000 abstract 1
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Przedmiotem zgłoszenia są sekwencje starterów pozwalające na analizę poziomu ekspresji genów w korzeniach jęczmienia oraz sposób identyfikacji genotypów jęczmienia o obniżonej tolerancji na cynk i kadm. Bardziej szczegółowo zgłoszenie dotyczy starterów do identyfikacji genów o identyfikatorach HORVU.MOREX.r2.5HG0395660, HORVU.MOREX.r2.1HG0017110, kodujących odpowiednio białko zawierające domenę U-box oraz peroksydazę. Dla każdego z genów zaprojektowano startery pozwalające na określenie ich poziomu ekspresji w korzeniach jęczmienia z użyciem zaproponowanego systemu badania poziomu ekspresji genów. Prezentowane zgłoszenie pozwoli na selekcję odmian roślin pod kątem tolerancji na stres cynku i kadmu, przez co możliwe będzie wykorzystanie w hodowli takich form, które nie będą akumulowały w swoich tkankach metali ciężkich.The subject of the application are primer sequences enabling the analysis of gene expression levels in barley roots and a method of identifying barley genotypes with reduced tolerance to zinc and cadmium. In more detail, the application concerns primers for identifying genes with the identifiers HORVU.MOREX.r2.5HG0395660, HORVU.MOREX.r2.1HG0017110, encoding a protein containing a U-box domain and a peroxidase, respectively. Primers were designed for each gene to determine their expression level in barley roots using the proposed gene expression level testing system. The presented application will allow the selection of plant varieties in terms of tolerance to zinc and cadmium stress, which will make it possible to use forms in breeding that will not accumulate heavy metals in their tissues.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL442115A PL442115A1 (en) | 2022-08-27 | 2022-08-27 | Primer sequences enabling analysis of gene expression levels in barley roots and method of identifying barley genotypes with reduced tolerance to zinc and cadmium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL442115A PL442115A1 (en) | 2022-08-27 | 2022-08-27 | Primer sequences enabling analysis of gene expression levels in barley roots and method of identifying barley genotypes with reduced tolerance to zinc and cadmium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL442115A1 true PL442115A1 (en) | 2024-03-04 |
Family
ID=90106999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL442115A PL442115A1 (en) | 2022-08-27 | 2022-08-27 | Primer sequences enabling analysis of gene expression levels in barley roots and method of identifying barley genotypes with reduced tolerance to zinc and cadmium |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL442115A1 (en) |
-
2022
- 2022-08-27 PL PL442115A patent/PL442115A1/en unknown
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