PL442104A1 - Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley genotypes with increased tolerance to cadmium - Google Patents

Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley genotypes with increased tolerance to cadmium

Info

Publication number
PL442104A1
PL442104A1 PL442104A PL44210422A PL442104A1 PL 442104 A1 PL442104 A1 PL 442104A1 PL 442104 A PL442104 A PL 442104A PL 44210422 A PL44210422 A PL 44210422A PL 442104 A1 PL442104 A1 PL 442104A1
Authority
PL
Poland
Prior art keywords
barley
horvu
morex
cadmium
gene expression
Prior art date
Application number
PL442104A
Other languages
Polish (pl)
Other versions
PL245380B1 (en
Inventor
Marek Marzec
Original Assignee
Uniwersytet Śląski W Katowicach
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Śląski W Katowicach filed Critical Uniwersytet Śląski W Katowicach
Priority to PL442104A priority Critical patent/PL245380B1/en
Publication of PL442104A1 publication Critical patent/PL442104A1/en
Publication of PL245380B1 publication Critical patent/PL245380B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia są sekwencje starterów pozwalające na analizę poziomu ekspresji genów w korzeniach jęczmienia oraz sposób identyfikacji genotypów jęczmienia o podwyższonej tolerancji na kadm. Bardziej szczegółowo zgłoszenie dotyczy starterów do identyfikacji genów o identyfikatorach HORVU.MOREX.r2.7HG0595000, HORVU.MOREX.r2.2HG0141160, HORVU.MOREX.r2.6HG0487860, HORVU.MOREX.r2.5HG0366120, HORVU.MOREX.r2.7HG0607750, kodujących odpowiednio czynnik transkrypcyjny szoku cieplnego, białko szoku cieplnego, białko oporności na choroby, białko lektyny podobne do konkanawaliny A oraz izomerazę glukozo-6-fosforanową. Dla każdego z genów zaprojektowano startery pozwalające na określenie ich poziomu ekspresji w korzeniach jęczmienia z użyciem zaproponowanego systemu badania poziomu ekspresji genów. Prezentowane zgłoszenie pozwoli na selekcję odmian roślin pod kątem tolerancji na stres kadmu, przez co możliwe będzie wykorzystanie w hodowli takich form, które nie będą akumulowały w swoich tkankach metali ciężkich.The subject of the application are primer sequences enabling the analysis of gene expression levels in barley roots and a method of identifying barley genotypes with increased tolerance to cadmium. More specifically, the application concerns primers for identifying genes with the identifiers HORVU.MOREX.r2.7HG0595000, HORVU.MOREX.r2.2HG0141160, HORVU.MOREX.r2.6HG0487860, HORVU.MOREX.r2.5HG0366120, HORVU.MOREX.r2.7HG06077 50, encoding heat shock transcription factor, heat shock protein, disease resistance protein, concanavalin A-like lectin protein, and glucose-6-phosphate isomerase, respectively. Primers were designed for each gene to determine their expression level in barley roots using the proposed gene expression level testing system. The presented application will allow the selection of plant varieties in terms of tolerance to cadmium stress, which will make it possible to use in breeding forms that will not accumulate heavy metals in their tissues.

PL442104A 2022-08-27 2022-08-27 Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley genotypes with increased tolerance to cadmium PL245380B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL442104A PL245380B1 (en) 2022-08-27 2022-08-27 Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley genotypes with increased tolerance to cadmium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL442104A PL245380B1 (en) 2022-08-27 2022-08-27 Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley genotypes with increased tolerance to cadmium

Publications (2)

Publication Number Publication Date
PL442104A1 true PL442104A1 (en) 2024-03-04
PL245380B1 PL245380B1 (en) 2024-07-15

Family

ID=90106967

Family Applications (1)

Application Number Title Priority Date Filing Date
PL442104A PL245380B1 (en) 2022-08-27 2022-08-27 Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley genotypes with increased tolerance to cadmium

Country Status (1)

Country Link
PL (1) PL245380B1 (en)

Also Published As

Publication number Publication date
PL245380B1 (en) 2024-07-15

Similar Documents

Publication Publication Date Title
Huyen et al. Introgression the salinity tolerance QTLs Saltol into AS996, the elite rice variety of Vietnam
WO2006076423A3 (en) Genes and uses for plant improvement
CN105838785B (en) SSR molecular marker and application with sesame black seed coat gene close linkage
Dimitrova-Doneva Correlation and Path-coefficient analysis of yield components in barley.
PL442106A1 (en) Primer sequences enabling analysis of gene expression levels in barley leaves and roots and method for identifying barley genotypes with increased tolerance to cadmium
PL442112A1 (en) Primer sequences enabling analysis of gene expression levels in barley leaves and roots and method for identifying barley genotypes with increased zinc tolerance
PL442118A1 (en) Primer sequences enabling analysis of gene expression levels in barley leaves and roots and method for identifying barley genotypes with increased tolerance to zinc and cadmium
PL442110A1 (en) Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley genotypes with increased tolerance to zinc
PL442103A1 (en) Primer sequences enabling analysis of gene expression levels in barley roots and method of identifying barley genotypes with reduced tolerance to cadmium
PL442108A1 (en) Primer sequences enabling analysis of the level of gene expression in barley leaves and method of identifying barley genotypes with increased tolerance to zinc
PL442102A1 (en) Primer sequences enabling analysis of gene expression levels in barley leaves and method of identifying barley genotypes with reduced tolerance to cadmium
PL442117A1 (en) Primer sequences enabling analysis of gene expression levels in barley leaves and roots and method for identifying barley genotypes with reduced tolerance to zinc and cadmium
PL442105A1 (en) Primer sequences enabling analysis of gene expression levels in barley leaves and roots and method for identifying barley genotypes with reduced tolerance to cadmium
PL442114A1 (en) Primer sequences enabling analysis of gene expression levels in barley leaves and method of identifying barley genotypes with increased tolerance to zinc and cadmium
PL442115A1 (en) Primer sequences enabling analysis of gene expression levels in barley roots and method of identifying barley genotypes with reduced tolerance to zinc and cadmium
PL442111A1 (en) Primer sequences enabling analysis of gene expression levels in barley leaves and roots and method for identifying barley genotypes with reduced zinc tolerance
PL442116A1 (en) Primer sequences enabling analysis of gene expression levels in barley roots and method of identifying barley genotypes with increased tolerance to zinc and cadmium
PL442109A1 (en) Primer sequences enabling analysis of gene expression levels in barley roots and method of identifying barley genotypes with reduced zinc tolerance
Adeboye et al. SSR analysis of genetic changes during artificial ageing of rice seeds stored under gene bank management
PL442113A1 (en) Primer sequences enabling analysis of gene expression levels in barley leaves and method of identifying barley genotypes with reduced tolerance to zinc and cadmium
ABD EL-GHANY et al. ASSESSMENT OF GENETIC DIVERSITY AMONG SOME EGYTIAN BARLEY CULTIVARS BASED ON SCoT AND ISSR MARKERS
CN110846421A (en) Application of gene and its detection kit
Johnston et al. Identifying Phytophthora sojae pathotypes through baiting and optimizing a new molecular method
PL442123A1 (en) Primer sequences enabling analysis of the level of gene expression in barley roots and method of identifying barley that was in contact with zinc
CN115873982A (en) Reference gene for gene expression in peony stalk development and primer and application thereof