PL426258A1 - Pair of oligonucleotide primers for detecting the ct2 dwarf gene and method for detecting the recessive ct2 dwarf gene in rye (Secale cereale L.) plants - Google Patents

Pair of oligonucleotide primers for detecting the ct2 dwarf gene and method for detecting the recessive ct2 dwarf gene in rye (Secale cereale L.) plants

Info

Publication number
PL426258A1
PL426258A1 PL426258A PL42625818A PL426258A1 PL 426258 A1 PL426258 A1 PL 426258A1 PL 426258 A PL426258 A PL 426258A PL 42625818 A PL42625818 A PL 42625818A PL 426258 A1 PL426258 A1 PL 426258A1
Authority
PL
Poland
Prior art keywords
detecting
pair
dwarf gene
rye
oligonucleotide primers
Prior art date
Application number
PL426258A
Other languages
Polish (pl)
Other versions
PL235221B1 (en
Inventor
Agnieszka Grądzielewska
Paweł Milczarski
Katarzyna Molik
Original Assignee
Uniwersytet Przyrodniczy W Lublinie
Zachodniopomorski Uniwersytet Technologiczny W Szczecinie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Przyrodniczy W Lublinie, Zachodniopomorski Uniwersytet Technologiczny W Szczecinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL426258A priority Critical patent/PL235221B1/en
Publication of PL426258A1 publication Critical patent/PL426258A1/en
Publication of PL235221B1 publication Critical patent/PL235221B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

Przedmiotem zgłoszenia jest para oligonukleotydowych starterów do wykrywania genu karłowatości ct2 w roślinach żyta o sekwencjach: MK61b_F1 TGTCTAAGTTGTCTTTCAGTGA MK64b_R4 GTTGCAAGTTCCCAAAGCCC. Zgłoszenie obejmuje także sposób wykrywania obecności genu karłowatości ct2 w roślinach żyta, w którym to sposobie polimorficzne fragmenty DNA sprzężone z badanym genem amplifikowane są w reakcji PCR z zastosowaniem pary starterów, po czym dokonuje się detekcji produktu amplifikacji, charakteryzujący się tym, że parę starterów stanowi para oligonukleotydowych starterów o sekwencjach MK61b_F1 TGTCTAAGTTGTCTTTCAGTGA MK64b_R4 GTTGCAAGTTCCCAAAGCCC przy czym w przypadku pary starterów MK61b_F1 i MK64b_R4 stosuje się marker d508041.The subject of the application is a pair of oligonucleotide primers for detecting the ct2 dwarf gene in rye with the sequences: MK61b_F1 TGTCTAAGTTGTCTTTCAGTGA MK64b_R4 GTTGCAAGTTCCCAAAGCCC. The application also covers a method for detecting the presence of the ct2 dwarf gene in rye plants, wherein the polymorphic DNA fragments linked to the test gene are amplified by PCR using a pair of primers, followed by detection of the amplification product, characterized in that the primer pair is a pair of oligonucleotide primers with the sequences MK61b_F1 TGTCTAAGTTGTCTTTCAGTGA MK64b_R4 GTTGCAAGTTCCCAAAGCCC where the marker d508041 is used for the primer pair MK61b_F1 and MK64b_R4.

PL426258A 2018-07-09 2018-07-09 Pair of oligonucleotide primers for detecting the ct2 dwarf gene and method for detecting the recessive ct2 dwarf gene in rye (Secale cereale L.) plants PL235221B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL426258A PL235221B1 (en) 2018-07-09 2018-07-09 Pair of oligonucleotide primers for detecting the ct2 dwarf gene and method for detecting the recessive ct2 dwarf gene in rye (Secale cereale L.) plants

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL426258A PL235221B1 (en) 2018-07-09 2018-07-09 Pair of oligonucleotide primers for detecting the ct2 dwarf gene and method for detecting the recessive ct2 dwarf gene in rye (Secale cereale L.) plants

Publications (2)

Publication Number Publication Date
PL426258A1 true PL426258A1 (en) 2020-01-13
PL235221B1 PL235221B1 (en) 2020-06-15

Family

ID=69161618

Family Applications (1)

Application Number Title Priority Date Filing Date
PL426258A PL235221B1 (en) 2018-07-09 2018-07-09 Pair of oligonucleotide primers for detecting the ct2 dwarf gene and method for detecting the recessive ct2 dwarf gene in rye (Secale cereale L.) plants

Country Status (1)

Country Link
PL (1) PL235221B1 (en)

Also Published As

Publication number Publication date
PL235221B1 (en) 2020-06-15

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