PL4204570T3 - Sposób wytwarzania rekombinownego białka w komórce gospodarza z zablokowanym metabolizmem ramnozy oraz wektory ekspresyjne, komórki gospodarza i ich rekombinowane białka - Google Patents

Sposób wytwarzania rekombinownego białka w komórce gospodarza z zablokowanym metabolizmem ramnozy oraz wektory ekspresyjne, komórki gospodarza i ich rekombinowane białka

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Publication number
PL4204570T3
PL4204570T3 PL21790425.9T PL21790425T PL4204570T3 PL 4204570 T3 PL4204570 T3 PL 4204570T3 PL 21790425 T PL21790425 T PL 21790425T PL 4204570 T3 PL4204570 T3 PL 4204570T3
Authority
PL
Poland
Prior art keywords
disabled
producing
well
expression vectors
host cell
Prior art date
Application number
PL21790425.9T
Other languages
English (en)
Inventor
David VIKSTRÖM
Nurzian ISMAIL
Patrik SAMUELSON
Kiavash MIRZADEH
Santhosh GUDISE
Maria KADOW
Kathleen SZEKÉR
Tagrid SALIH
Mariusz BARASZKIEWICZ
Siavash BASHIRI
Original Assignee
Stada Arzneimittel Ag
Xbrane Biopharma Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stada Arzneimittel Ag, Xbrane Biopharma Ab filed Critical Stada Arzneimittel Ag
Publication of PL4204570T3 publication Critical patent/PL4204570T3/pl

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/20Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55Fab or Fab'

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
PL21790425.9T 2020-10-09 2021-10-08 Sposób wytwarzania rekombinownego białka w komórce gospodarza z zablokowanym metabolizmem ramnozy oraz wektory ekspresyjne, komórki gospodarza i ich rekombinowane białka PL4204570T3 (pl)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20201096.3A EP3981879A1 (en) 2020-10-09 2020-10-09 Method of producing a recombinant protein in a host cell which has a disabled rhamnose metabolism as well as expression vectors, host cells and recombinant proteins thereof
PCT/EP2021/077820 WO2022074182A1 (en) 2020-10-09 2021-10-08 Method of producing a recombinant protein in a host cell which has a disabled rhamnose metabolism as well as expression vectors, host cells and recombinant proteins thereof

Publications (1)

Publication Number Publication Date
PL4204570T3 true PL4204570T3 (pl) 2025-10-06

Family

ID=72826766

Family Applications (1)

Application Number Title Priority Date Filing Date
PL21790425.9T PL4204570T3 (pl) 2020-10-09 2021-10-08 Sposób wytwarzania rekombinownego białka w komórce gospodarza z zablokowanym metabolizmem ramnozy oraz wektory ekspresyjne, komórki gospodarza i ich rekombinowane białka

Country Status (14)

Country Link
US (1) US20230340096A1 (pl)
EP (2) EP3981879A1 (pl)
JP (1) JP2023549432A (pl)
KR (1) KR20230079228A (pl)
CN (1) CN116323950A (pl)
AU (1) AU2021357360A1 (pl)
CA (1) CA3193217A1 (pl)
ES (1) ES3036687T3 (pl)
GE (2) GEP20247669B (pl)
IL (1) IL301642A (pl)
MX (1) MX2023003819A (pl)
PL (1) PL4204570T3 (pl)
WO (1) WO2022074182A1 (pl)
ZA (1) ZA202303775B (pl)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20240099199A (ko) * 2021-09-24 2024-06-28 엑스브레인 바이오파마 에이비 재조합 단백질을 발현시키기 위한 dna 구조체 및 숙주 세포

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
CN101090969B (zh) * 2004-12-07 2013-01-16 隆萨股份公司 鼠李糖启动子表达系统及用途
EP2268818B1 (en) 2008-02-29 2012-02-08 Xbrane Bioscience Aktiebolag Expression system for proteins
EP2362896A2 (en) * 2008-11-11 2011-09-07 Danisco US Inc. Bacillus subtilisin comprising one or more combinable mutations
WO2012041899A1 (en) * 2010-09-28 2012-04-05 Xbrane Bioscience Ab Fusion protein for secretory protein expression
BR112019023795A2 (pt) * 2017-05-19 2020-07-28 Council Of Scientific & Industrial Research método para produzir ranibizumabe humanizado recombinante redobrado

Also Published As

Publication number Publication date
EP4204570C0 (en) 2025-06-04
ZA202303775B (en) 2025-07-30
AU2021357360A1 (en) 2023-05-04
CN116323950A (zh) 2023-06-23
KR20230079228A (ko) 2023-06-05
MX2023003819A (es) 2023-08-16
EP4204570B1 (en) 2025-06-04
JP2023549432A (ja) 2023-11-24
CA3193217A1 (en) 2022-04-14
EP3981879A1 (en) 2022-04-13
GEP20247669B (en) 2024-09-25
IL301642A (en) 2023-05-01
US20230340096A1 (en) 2023-10-26
AU2021357360A9 (en) 2023-07-06
ES3036687T3 (en) 2025-09-23
WO2022074182A9 (en) 2022-06-16
EP4204570A1 (en) 2023-07-05
WO2022074182A1 (en) 2022-04-14
GEAP202416228A (en) 2024-05-13

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