PL403939A1 - Aptamery DNA rozpoznające metkę histydynową oraz ich zastosowanie - Google Patents

Aptamery DNA rozpoznające metkę histydynową oraz ich zastosowanie

Info

Publication number
PL403939A1
PL403939A1 PL40393913A PL40393913A PL403939A1 PL 403939 A1 PL403939 A1 PL 403939A1 PL 40393913 A PL40393913 A PL 40393913A PL 40393913 A PL40393913 A PL 40393913A PL 403939 A1 PL403939 A1 PL 403939A1
Authority
PL
Poland
Prior art keywords
histidine tag
dna aptamers
aptamers recognizing
recognizing
seq
Prior art date
Application number
PL40393913A
Other languages
English (en)
Other versions
PL225448B1 (pl
Inventor
Filip Bartnicki
Ewa Kowalska
Katarzyna Pels
Wojciech Strzałka
Original Assignee
Uniwersytet Jagielloński
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Jagielloński filed Critical Uniwersytet Jagielloński
Priority to PL403939A priority Critical patent/PL225448B1/pl
Priority to US14/780,635 priority patent/US10023870B2/en
Priority to PCT/PL2014/050026 priority patent/WO2014185802A1/en
Priority to EP14736043.2A priority patent/EP2997147B1/en
Priority to JP2016513897A priority patent/JP6302542B2/ja
Priority to KR1020157025684A priority patent/KR102133618B1/ko
Priority to CN201480018979.3A priority patent/CN105189753B/zh
Publication of PL403939A1 publication Critical patent/PL403939A1/pl
Publication of PL225448B1 publication Critical patent/PL225448B1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Ujawniono aptamer DNA wykazujący powinowactwo do metki His, zawierający sekwencję nukleotydową wybraną spośród Sekw Id Nr 1 i Sekw Id Nr 2 oraz jego zastosowania.
PL403939A 2013-05-17 2013-05-17 Aptamer DNA rozpoznający metkę histydynową oraz jego zastosowanie PL225448B1 (pl)

Priority Applications (7)

Application Number Priority Date Filing Date Title
PL403939A PL225448B1 (pl) 2013-05-17 2013-05-17 Aptamer DNA rozpoznający metkę histydynową oraz jego zastosowanie
US14/780,635 US10023870B2 (en) 2013-05-17 2014-05-17 DNA aptamers binding the histidine tag and their application
PCT/PL2014/050026 WO2014185802A1 (en) 2013-05-17 2014-05-17 Dna aptamers binding the histidine tag and their application
EP14736043.2A EP2997147B1 (en) 2013-05-17 2014-05-17 Dna aptamers binding the histidine tag and their application
JP2016513897A JP6302542B2 (ja) 2013-05-17 2014-05-17 ヒスチジンタグに結合するｄｎａアプタマーおよびその用途
KR1020157025684A KR102133618B1 (ko) 2013-05-17 2014-05-17 히스티딘 태그에 결합하는 dna 앱타머와 그것의 용도
CN201480018979.3A CN105189753B (zh) 2013-05-17 2014-05-17 Dna适配体结合组氨酸标签及其应用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL403939A PL225448B1 (pl) 2013-05-17 2013-05-17 Aptamer DNA rozpoznający metkę histydynową oraz jego zastosowanie

Publications (2)

Publication Number Publication Date
PL403939A1 true PL403939A1 (pl) 2014-11-24
PL225448B1 PL225448B1 (pl) 2017-04-28

Family

ID=51134183

Family Applications (1)

Application Number Title Priority Date Filing Date
PL403939A PL225448B1 (pl) 2013-05-17 2013-05-17 Aptamer DNA rozpoznający metkę histydynową oraz jego zastosowanie

Country Status (7)

Country Link
US (1) US10023870B2 (pl)
EP (1) EP2997147B1 (pl)
JP (1) JP6302542B2 (pl)
KR (1) KR102133618B1 (pl)
CN (1) CN105189753B (pl)
PL (1) PL225448B1 (pl)
WO (1) WO2014185802A1 (pl)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL226916B1 (pl) * 2014-12-16 2017-09-29 Univ Jagielloński Aptamer DNA rozpoznajacy ludzkie białko PCNA oraz jego zastosowanie
CA3031386A1 (en) * 2016-07-28 2018-02-01 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Method for obtaining aptamers
CN109055382B (zh) * 2018-08-27 2022-04-12 温州医科大学附属第一医院 可特异性结合6x组氨酸标签的核酸适配体及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329742B2 (en) * 2003-09-04 2008-02-12 The Regents Of The University Of California Aptamers and methods for their in vitro selection and uses thereof
KR101178847B1 (ko) * 2008-12-08 2012-08-31 한국전자통신연구원 가상공간과 연계된 다중 로봇 제어 장치 및 방법
CN102229932B (zh) 2011-06-03 2013-02-27 湖南大学 可识别hcv e1e2蛋白的核酸适体、核酸适体的衍生物及其筛选方法和应用
KR101333158B1 (ko) * 2011-10-05 2013-11-27 경희대학교 산학협력단 히스티딘 태그를 가진 단백질의 정제를 위한 고형 지지체 및 이를 이용한 정제 방법
CN102719430B (zh) * 2012-06-13 2013-06-12 湖南大学 一种检测组氨酸标签重组蛋白的核酸适配体分子信标探针及其检测方法

Also Published As

Publication number Publication date
KR102133618B1 (ko) 2020-07-14
CN105189753A (zh) 2015-12-23
US10023870B2 (en) 2018-07-17
JP2016518852A (ja) 2016-06-30
PL225448B1 (pl) 2017-04-28
CN105189753B (zh) 2018-06-12
US20160060631A1 (en) 2016-03-03
EP2997147B1 (en) 2018-03-28
WO2014185802A1 (en) 2014-11-20
KR20160026825A (ko) 2016-03-09
JP6302542B2 (ja) 2018-03-28
EP2997147A1 (en) 2016-03-23

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