PL2914743T3 - Przechwytywanie, wykrywanie i oznaczanie ilościowe małego RNA - Google Patents

Przechwytywanie, wykrywanie i oznaczanie ilościowe małego RNA

Info

Publication number
PL2914743T3
PL2914743T3 PL13795633T PL13795633T PL2914743T3 PL 2914743 T3 PL2914743 T3 PL 2914743T3 PL 13795633 T PL13795633 T PL 13795633T PL 13795633 T PL13795633 T PL 13795633T PL 2914743 T3 PL2914743 T3 PL 2914743T3
Authority
PL
Poland
Prior art keywords
quantification
detection
small rna
rna capture
capture
Prior art date
Application number
PL13795633T
Other languages
English (en)
Inventor
Linda Wong
Caifu Chen
Yalei WU
Shoulian Dong
Chunmei Liu
Original Assignee
Life Technologies Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corporation filed Critical Life Technologies Corporation
Publication of PL2914743T3 publication Critical patent/PL2914743T3/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PL13795633T 2012-11-02 2013-11-04 Przechwytywanie, wykrywanie i oznaczanie ilościowe małego RNA PL2914743T3 (pl)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201261721968P 2012-11-02 2012-11-02
US201261740242P 2012-12-20 2012-12-20
PCT/US2013/068350 WO2014071322A1 (en) 2012-11-02 2013-11-04 Small RNA Capture, Detection and Quantification
EP13795633.0A EP2914743B1 (en) 2012-11-02 2013-11-04 Small rna capture, detection and quantification

Publications (1)

Publication Number Publication Date
PL2914743T3 true PL2914743T3 (pl) 2020-05-18

Family

ID=49585636

Family Applications (2)

Application Number Title Priority Date Filing Date
PL13795633T PL2914743T3 (pl) 2012-11-02 2013-11-04 Przechwytywanie, wykrywanie i oznaczanie ilościowe małego RNA
PL17186097T PL3260558T3 (pl) 2012-11-02 2013-11-04 Nowe kompozycje i sposoby poprawy specyficzności pcr

Family Applications After (1)

Application Number Title Priority Date Filing Date
PL17186097T PL3260558T3 (pl) 2012-11-02 2013-11-04 Nowe kompozycje i sposoby poprawy specyficzności pcr

Country Status (7)

Country Link
US (7) US9416405B2 (pl)
EP (5) EP3628746B1 (pl)
CN (2) CN111793621B (pl)
DK (2) DK2914743T3 (pl)
ES (3) ES2749463T3 (pl)
PL (2) PL2914743T3 (pl)
WO (2) WO2014071315A1 (pl)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG185544A1 (en) * 2010-05-14 2012-12-28 Fluidigm Corp Nucleic acid isolation methods
US9528107B2 (en) * 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
WO2014071315A1 (en) 2012-11-02 2014-05-08 Life Technologies Corporation Novel compositions, methods and kits for enhancing pcr specificity
EP2971033B8 (en) 2013-03-15 2019-07-10 ModernaTX, Inc. Manufacturing methods for production of rna transcripts
EP4279610A3 (en) 2013-03-15 2024-01-03 ModernaTX, Inc. Ribonucleic acid purification
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
SG10201708498VA (en) 2013-04-17 2017-11-29 Agency Science Tech & Res Method for generating extended sequence reads
JP7058502B2 (ja) * 2014-04-11 2022-04-22 レッドボールト バイオサイエンシズ エルピー ゲノム適用および治療適用のための、核酸分子のクローン複製および増幅のためのシステムおよび方法
CN114250278B (zh) * 2015-03-13 2024-08-06 生命技术公司 捕获、检测和定量小rna的方法、组合物与试剂盒
WO2016164866A1 (en) 2015-04-10 2016-10-13 Hudsonalpha Institute For Biotechnology METHOD FOR BLOCKING miRNA
US10844427B2 (en) 2015-04-30 2020-11-24 Thomas Jefferson University Four-leaf clover qRT-PCR: an efficient and convenient method for selective quantification of mature tRNA
WO2017035090A1 (en) * 2015-08-21 2017-03-02 Thomas Jefferson University Dumbbell-pcr: a method to quantify specific small rna variants with a single nucleotide resolution at terminal sequences
US10287625B2 (en) * 2016-03-18 2019-05-14 Norgen Biotek Corp. Methods and kits for separating nucleic acids by size
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
CN110139931B (zh) * 2016-08-30 2024-06-11 元素生物科学公司 用于定相测序的方法和组合物
CN107151695B (zh) * 2016-12-08 2021-11-09 青岛大学 用于检测急性心肌缺血疾病的piRNA组合及其检测方法和应用
JP7048609B2 (ja) 2016-12-09 2022-04-05 ボリアル ジェノミクス， インコーポレイテッド 連結型ライゲーション
WO2019236726A1 (en) 2018-06-06 2019-12-12 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
US12098419B2 (en) 2018-08-23 2024-09-24 Ncan Genomics, Inc. Linked target capture and ligation
EP4538389A3 (en) 2019-01-03 2025-07-30 Ncan Genomics, Inc. Linked target capture
US11390915B2 (en) * 2019-04-26 2022-07-19 New England Biolabs, Inc. Polynucleotide adapter design for reduced bias
GB201911421D0 (en) * 2019-08-09 2019-09-25 Dnae Diagnostics Ltd Methods of multiplexed isothermal amplification of nucleic acid sequences
US11515117B2 (en) 2020-08-28 2022-11-29 GE Precision Healthcare LLC Biased cathode assembly of an X-ray tube with improved thermal management and a method of manufacturing same
US20240344056A1 (en) * 2021-06-04 2024-10-17 Somagenics, Inc. Improved method of sequencing library preparation for short dna
US12601008B2 (en) 2021-11-04 2026-04-14 Florida State University Research Foundation, Inc. Using hairpin formation to identify DNA and RNA sequences having a target nucleic acid sequence
CN114295594B (zh) * 2021-12-06 2023-09-19 贵州理工学院 一种基于分子信标筛选三螺旋DNA嵌入剂的“turn on”型荧光传感器
CN114252602B (zh) * 2021-12-22 2023-09-12 清华大学深圳国际研究生院 微流控芯片、基于微流控芯片的检测系统及细菌的检测方法
EP4457356A4 (en) * 2021-12-24 2025-10-29 Pacbridge Partners Ii Invest Co Ltd COMPOSITIONS AND METHODS FOR IDENTIFYING GENE FUSIONS
US20250092448A1 (en) * 2022-01-12 2025-03-20 Shoulian Dong Methods and compositions for nucleic acid amplifications
EP4549583A4 (en) * 2022-06-29 2026-06-17 Xenohelix Co Ltd METHOD FOR DETECTING TARGET RNA, COMPRISING TREATMENT WITH A BLOCKING NUCLEIC ACID

Family Cites Families (109)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
CA1190838A (en) 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US6127155A (en) 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5244797B1 (en) 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5270179A (en) 1989-08-10 1993-12-14 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'- to-5' exonuclease activity
US5047342A (en) 1989-08-10 1991-09-10 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase
EP0540693B1 (en) 1990-07-24 1999-01-20 F. Hoffmann-La Roche Ag THE REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING $i(IN VITRO) NUCLEIC ACID AMPLIFICATION USING MODIFIED NUCLEIC ACID BASES
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JP2709311B2 (ja) 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
WO1992006188A2 (en) 1990-10-05 1992-04-16 Barnes Wayne M Thermostable dna polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE69430665T2 (de) 1993-01-15 2002-11-21 The Public Health Research Institute Of The City Of New York, Inc. Empfindlicher nukleinsäure-sandwichhybridisierungs-assay und kits
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5767259A (en) 1994-12-27 1998-06-16 Naxcor Oligonucleotides containing base-free linking groups with photoactivatable side chains
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6821727B1 (en) 1993-11-15 2004-11-23 Applera Corporation Hybridization assay using self-quenching fluorescence probe
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
CA2185239C (en) 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US6153425A (en) 1995-07-13 2000-11-28 Xtrana, Inc. Self-contained device integrating nucleic acid extraction, amplification and detection
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5770365A (en) * 1995-08-25 1998-06-23 Tm Technologies, Inc. Nucleic acid capture moieties
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
AU730633B2 (en) 1996-05-29 2001-03-08 Phillip Belgrader Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
EP0977779A1 (en) 1997-04-21 2000-02-09 Schering Corporation Mammalian cytokines; related reagents and methods
CA2287542A1 (en) 1997-04-22 1998-10-29 Invitrogen Corporation Methods for the production of aslv reverse transcriptases composed of multiple subunits
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
AU1366299A (en) 1997-10-27 1999-05-17 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6238927B1 (en) 1998-10-05 2001-05-29 Mosaic Technologies, Incorporated Reverse displacement assay for detection of nucleic acid sequences
US6383752B1 (en) 1999-03-31 2002-05-07 Hybridon, Inc. Pseudo-cyclic oligonucleobases
US20070059752A1 (en) * 1999-06-09 2007-03-15 Biosearch Technologies, Inc. Fluorescence energy transfer probes with stabilized conformations
US6528254B1 (en) 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US6706476B1 (en) * 2000-08-22 2004-03-16 Azign Bioscience A/S Process for amplifying and labeling single stranded cDNA by 5′ ligated adaptor mediated amplification
US6569927B1 (en) 2000-10-06 2003-05-27 Uniroyal Chemical Company, Inc. Thermoplastic resins stabilized by blends of sterically hindered phenols, secondary amines, and lactones
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
WO2003025132A2 (en) 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
US6593091B2 (en) 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
US6589250B2 (en) 2001-11-20 2003-07-08 Stephen A. Schendel Maxillary distraction device
WO2003048309A2 (en) 2001-11-30 2003-06-12 Applera Corporation Thermus thermophilus nucleic acid polymerases
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
GB0218215D0 (en) * 2002-08-06 2002-09-11 Tepnel Medical Ltd Amplification of nucleic acids
EP1426448A1 (en) * 2002-12-03 2004-06-09 bioMérieux BV Method for lowering the effects of sequence variations in a diagnostic hybridization assay, probe for use in the assay and assay
JP2006508662A (ja) 2002-12-04 2006-03-16 アプレラ コーポレイション ポリヌクレオチドの多重増幅
US7282333B2 (en) * 2003-10-20 2007-10-16 Promega Corporation Methods and compositions for nucleic acid analysis
US8192937B2 (en) * 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
DK1735459T3 (da) 2004-04-07 2012-05-29 Exiqon As Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er
US7387887B2 (en) 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
US7361465B2 (en) 2004-09-07 2008-04-22 Applera Corporation Methods and compositions for tailing and amplifying RNA
US7579153B2 (en) 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
US20070009924A1 (en) 2005-01-28 2007-01-11 Lee Jun E Thermococcus zilligii DNA polymerases and variants thereof
WO2006084201A2 (en) 2005-02-04 2006-08-10 Genosensor Corporation Method of isolating, labeling and profiling small rna and whole-genome transcripts
US7824890B2 (en) 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
US20060223066A1 (en) 2005-03-29 2006-10-05 Applera Corporation Methods for normalizing and for identifying small nucleic acids
US7456281B2 (en) * 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
US20070054287A1 (en) * 2005-05-31 2007-03-08 Applera Corporation Method for identifying medically important cell populations using micro rna as tissue specific biomarkers
DK1907571T3 (en) * 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
US20060292578A1 (en) 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
AU2006265896B2 (en) * 2005-06-30 2012-05-31 Archemix Corp. Materials and methods for the generation of fully 2'-modified nucleic acid transcripts
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
WO2007025281A2 (en) * 2005-08-24 2007-03-01 Applera Corporation A method to quantify sirnas, mirnas and polymorphic mirnas
EP2021506B1 (en) * 2006-04-28 2016-03-16 Igor Kutyavin Use of products of pcr amplification carrying elements of secondary structure to improve pcr-based nucleic acid detection
WO2008029295A2 (en) 2006-06-30 2008-03-13 Rosetta Genomics Ltd A method for detecting nucleic acids
DE102006038113A1 (de) 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
US20080248469A1 (en) * 2006-08-16 2008-10-09 Applera Corporation Methods for Identifying Nucleotides of Interest in Target Polynucleotides
EP2079754B1 (en) * 2006-10-04 2017-03-15 Third Wave Technologies, Inc. Snap-back primers and detectable hairpin structures
US8383370B2 (en) 2007-01-30 2013-02-26 The Rockefeller University Modified RNA ligase for efficient 3′ modification of RNA
EP2121983A2 (en) * 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US20080194416A1 (en) * 2007-02-08 2008-08-14 Sigma Aldrich Detection of mature small rna molecules
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
EP1978111B1 (en) * 2007-04-02 2013-03-27 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Pseudomonas aeruginosa
US20090061424A1 (en) 2007-08-30 2009-03-05 Sigma-Aldrich Company Universal ligation array for analyzing gene expression or genomic variations
US20090215057A1 (en) * 2007-11-19 2009-08-27 Reimo Tetzner Analysis molecule and method for the analysis of a nucleotide position in a nucleic acid
US20090197254A1 (en) 2007-12-14 2009-08-06 Ming-Chou Lee Variant scorpion primers for nucleic acid amplification and detection
JP5685085B2 (ja) * 2008-01-14 2015-03-18 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー リボ核酸を検出するための組成物、方法およびキット
US8399221B2 (en) 2008-11-04 2013-03-19 Sabiosciences Corporation Methods for detection and quantitation of small RNAs
SG172965A1 (en) 2009-01-13 2011-08-29 Fluidigm Corp Single-cell nucleic acid analysis
WO2010085966A2 (en) 2009-02-02 2010-08-05 Exiqon A/S Method for quantification of small rna species
EP2534263B1 (en) * 2010-02-09 2020-08-05 Unitaq Bio Methods and compositions for universal detection of nucleic acids
US20130045885A1 (en) * 2010-02-18 2013-02-21 Univeristy Of South Florida Materials and methods for profiling micrornas
US9255291B2 (en) * 2010-05-06 2016-02-09 Bioo Scientific Corporation Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing
WO2011146942A1 (en) 2010-05-21 2011-11-24 The Translational Genomics Research Institute Methods and kits to analyze microrna by nucleic acid sequencing
US8883421B2 (en) 2010-09-10 2014-11-11 New England Biolabs, Inc. Method for reducing adapter-dimer formation
US8575071B2 (en) 2010-11-03 2013-11-05 Illumina, Inc. Reducing adapter dimer formation
CN103492587B (zh) 2010-12-13 2015-02-18 生命技术公司 利用通过多磷酸分解作用(app)反应的活化聚合核酸
CN103261416B (zh) 2010-12-16 2016-08-10 安捷伦科技有限公司 利用真核tRNA连接酶的连接方法
US9677130B2 (en) 2011-02-15 2017-06-13 Institute For Systems Biology Methods to detect and quantify RNA
CN102676637B (zh) 2011-03-08 2016-02-03 生元科技有限公司 一种高专一性的测定小rna的方法
EP3301188B1 (en) * 2012-04-16 2020-10-07 Life Technologies Corporation Methods for the preparation of rna libraries
WO2014071315A1 (en) 2012-11-02 2014-05-08 Life Technologies Corporation Novel compositions, methods and kits for enhancing pcr specificity
CN114250278B (zh) 2015-03-13 2024-08-06 生命技术公司 捕获、检测和定量小rna的方法、组合物与试剂盒

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