PL2855676T3 - Kompozycje i sposoby ekspresji i oczyszczania peptydów z wykorzystaniem systemu sekrecji typu iii - Google Patents

Kompozycje i sposoby ekspresji i oczyszczania peptydów z wykorzystaniem systemu sekrecji typu iii

Info

Publication number
PL2855676T3
PL2855676T3 PL13798228T PL13798228T PL2855676T3 PL 2855676 T3 PL2855676 T3 PL 2855676T3 PL 13798228 T PL13798228 T PL 13798228T PL 13798228 T PL13798228 T PL 13798228T PL 2855676 T3 PL2855676 T3 PL 2855676T3
Authority
PL
Poland
Prior art keywords
purification
compositions
methods
secretion system
peptide expression
Prior art date
Application number
PL13798228T
Other languages
English (en)
Polish (pl)
Inventor
Kelly T. Hughes
Baldomero M. Olivera
Original Assignee
University Of Utah Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Utah Research Foundation filed Critical University Of Utah Research Foundation
Publication of PL2855676T3 publication Critical patent/PL2855676T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/255—Salmonella (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43522—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from scorpions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Insects & Arthropods (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PL13798228T 2012-05-30 2013-05-30 Kompozycje i sposoby ekspresji i oczyszczania peptydów z wykorzystaniem systemu sekrecji typu iii PL2855676T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261689284P 2012-05-30 2012-05-30
EP13798228.6A EP2855676B1 (en) 2012-05-30 2013-05-30 COMPOSITIONS AND METHODS FOR PEPTIDE EXPRESSION AND PURIFICATION USING A TYPE lll SECRETION SYSTEM
PCT/US2013/043384 WO2013181404A2 (en) 2012-05-30 2013-05-30 COMPOSITIONS AND METHODS FOR PEPTIDE EXPRESSION AND PURIFICATION USING A TYPE lll SECRETION SYSTEM

Publications (1)

Publication Number Publication Date
PL2855676T3 true PL2855676T3 (pl) 2021-07-05

Family

ID=49674058

Family Applications (1)

Application Number Title Priority Date Filing Date
PL13798228T PL2855676T3 (pl) 2012-05-30 2013-05-30 Kompozycje i sposoby ekspresji i oczyszczania peptydów z wykorzystaniem systemu sekrecji typu iii

Country Status (7)

Country Link
US (2) US9630997B2 (cg-RX-API-DMAC10.html)
EP (1) EP2855676B1 (cg-RX-API-DMAC10.html)
JP (1) JP6496243B2 (cg-RX-API-DMAC10.html)
DK (1) DK2855676T3 (cg-RX-API-DMAC10.html)
ES (1) ES2861275T3 (cg-RX-API-DMAC10.html)
PL (1) PL2855676T3 (cg-RX-API-DMAC10.html)
WO (1) WO2013181404A2 (cg-RX-API-DMAC10.html)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6496243B2 (ja) * 2012-05-30 2019-04-03 ユニヴァーシティー オブ ユタ リサーチ ファウンデーション Ｉｉｉ型分泌系を使用したペプチド発現および精製のための組成物および方法
JP6938482B2 (ja) * 2015-10-02 2021-09-22 ユニバーシティー オブ ユタ リサーチ ファンデーション 調節可能なリボソーム翻訳速度のための組成物および使用法
US10849938B2 (en) 2017-09-13 2020-12-01 ZBiotics Company Gene expression system for probiotic microorganisms
CN108837152B (zh) * 2018-05-23 2021-06-22 浙江大学 鞭毛主调控基因flhDC或其表达产物作为靶点在抗菌药物开发中的应用
WO2020180800A1 (en) * 2019-03-04 2020-09-10 The Board Of Trustees Of The Leland Stanford Junior University Engineered cd47 extracellular domain for bioconjugation
JP2023546022A (ja) 2020-10-08 2023-11-01 ジーバイオティクス カンパニー 腸内での繊維合成酵素の発現のための操作されたプロバイオティクス
KR102933213B1 (ko) * 2022-07-21 2026-03-03 충남대학교산학협력단 코난토킨-g의 분비를 위한 재조합 발현 벡터 및 이로 형질전환된 약독화 살모넬라 균주
KR20240013955A (ko) * 2022-07-21 2024-01-31 충남대학교산학협력단 멜리틴 분비를 위한 재조합 발현 벡터 및 이로 형질전환된 약독화 살모넬라 균주
KR20240015034A (ko) * 2022-07-25 2024-02-02 충남대학교산학협력단 Stichodactyla 독소 분비를 위한 재조합 발현 벡터및 이로 형질전환된 약독화 살모넬라 균주
KR20240015029A (ko) * 2022-07-25 2024-02-02 충남대학교산학협력단 카탈레이스 분비를 위한 재조합 발현 벡터 및 이로형질전환된 대장균 균주

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7659102B2 (en) * 2001-02-21 2010-02-09 Verenium Corporation Amylases, nucleic acids encoding them and methods for making and using them
US20040033608A1 (en) * 2001-10-10 2004-02-19 Purdue Research Foundation Plasmids, strains, and methods of use
WO2003033536A2 (en) * 2001-10-19 2003-04-24 Paradigm Therapeutics Limited G-protein coupled receptor
US20120027786A1 (en) * 2010-02-23 2012-02-02 Massachusetts Institute Of Technology Genetically programmable pathogen sense and destroy
JP6496243B2 (ja) * 2012-05-30 2019-04-03 ユニヴァーシティー オブ ユタ リサーチ ファウンデーション Ｉｉｉ型分泌系を使用したペプチド発現および精製のための組成物および方法

Also Published As

Publication number Publication date
EP2855676A4 (en) 2016-04-06
US20150225466A1 (en) 2015-08-13
ES2861275T3 (es) 2021-10-06
JP2015523066A (ja) 2015-08-13
JP6496243B2 (ja) 2019-04-03
DK2855676T3 (da) 2021-04-12
US20180009851A1 (en) 2018-01-11
US9630997B2 (en) 2017-04-25
HK1209158A1 (en) 2016-03-24
WO2013181404A3 (en) 2015-06-04
WO2013181404A2 (en) 2013-12-05
EP2855676B1 (en) 2021-01-13
EP2855676A2 (en) 2015-04-08
US10800818B2 (en) 2020-10-13

Similar Documents

Publication Publication Date Title
AP3864A (en) Pharmaceutical composition
EP2928562A4 (en) PEPTIDE THERAPEUTIC PRODUCTS AND METHODS OF USE
SG11201400432VA (en) Polypeptide vaccine
IL231604B (en) Therapeutic peptides
EP2857033A4 (en) PEPTIDE WITH SKIN PERMEATION
ZA201306118B (en) Lyophilized formulations
PL2718498T3 (pl) System mocowania szyn
GB201111183D0 (en) Peptide
SG11201405578TA (en) Pharmaceutical composition containing peptide
SG10201606218WA (en) Purified Proteins
GB201115910D0 (en) Peptides
ZA201308157B (en) Pharmaceutical composition
PT2922530T (pt) Formulações de acetato de caspofungina
EP2709593A4 (en) PHARMACEUTICAL COMPOSITION OF MULTIPLE PARTICLES
GB201218278D0 (en) Translocating peptide
EP2727593A4 (en) PHARMACEUTICAL COMPOSITION CONTAINING NICORANDIL
GB201200555D0 (en) Peptide
EP2711010A4 (en) PHARMACEUTICAL COMPOSITION
GB201104058D0 (en) Oral irrigation system toothspa
HUP1100444A2 (en) Pharmaceutical composition
GB201223114D0 (en) Novel peptide
PL2495467T3 (pl) System uszczelniający
HUP1100445A2 (en) Pharmaceutical composition
EP2891663A4 (en) PSF1-DERIVED PEPTIDE
GB201221414D0 (en) Trans-locating peptide