PL2828218T3 - Sposoby obniżania współczynnika błędów masywnie równoległej sekwencji dna z wykorzystaniem duplex consensus sequencing - Google Patents

Sposoby obniżania współczynnika błędów masywnie równoległej sekwencji dna z wykorzystaniem duplex consensus sequencing

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Publication number
PL2828218T3
PL2828218T3 PL13764186T PL13764186T PL2828218T3 PL 2828218 T3 PL2828218 T3 PL 2828218T3 PL 13764186 T PL13764186 T PL 13764186T PL 13764186 T PL13764186 T PL 13764186T PL 2828218 T3 PL2828218 T3 PL 2828218T3
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Poland
Prior art keywords
sequencing
lowering
methods
error rate
massively parallel
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PL13764186T
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English (en)
Inventor
Michael Schmitt
Jesse Salk
Lawrence A. Loeb
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University Of Washington Through Its Center For Commercialization
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49223282&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL2828218(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University Of Washington Through Its Center For Commercialization filed Critical University Of Washington Through Its Center For Commercialization
Publication of PL2828218T3 publication Critical patent/PL2828218T3/pl

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
PL13764186T 2012-03-20 2013-03-15 Sposoby obniżania współczynnika błędów masywnie równoległej sekwencji dna z wykorzystaniem duplex consensus sequencing PL2828218T3 (pl)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261613413P 2012-03-20 2012-03-20
US201261625319P 2012-04-17 2012-04-17
US201261625623P 2012-04-17 2012-04-17
EP13764186.6A EP2828218B9 (en) 2012-03-20 2013-03-15 Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
PCT/US2013/032665 WO2013142389A1 (en) 2012-03-20 2013-03-15 Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing

Publications (1)

Publication Number Publication Date
PL2828218T3 true PL2828218T3 (pl) 2021-01-11

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PL13764186T PL2828218T3 (pl) 2012-03-20 2013-03-15 Sposoby obniżania współczynnika błędów masywnie równoległej sekwencji dna z wykorzystaniem duplex consensus sequencing

Country Status (8)

Country Link
US (32) US9752188B2 (pl)
EP (3) EP3744857A1 (pl)
DK (1) DK2828218T3 (pl)
ES (1) ES2828661T3 (pl)
HU (1) HUE051845T2 (pl)
PL (1) PL2828218T3 (pl)
PT (1) PT2828218T (pl)
WO (1) WO2013142389A1 (pl)

Families Citing this family (168)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
ES2555106T3 (es) 2010-04-05 2015-12-29 Prognosys Biosciences, Inc. Ensayos biológicos codificados espacialmente
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP3907297A1 (en) 2011-04-15 2021-11-10 The Johns Hopkins University Safe sequencing system
PL2814959T3 (pl) * 2012-02-17 2018-07-31 Fred Hutchinson Cancer Research Center Kompozycje i sposoby do dokładnej identyfikacji mutacji
EP2825675B1 (en) 2012-03-13 2017-12-27 Patel, Abhijit Ajit Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
HUE051845T2 (hu) * 2012-03-20 2021-03-29 Univ Washington Through Its Center For Commercialization Módszerek a tömegesen párhuzamos DNS-szekvenálás hibaarányának csökkentésére duplex konszenzus szekvenálással
WO2013181170A1 (en) 2012-05-31 2013-12-05 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP2893040B1 (en) 2012-09-04 2019-01-02 Guardant Health, Inc. Methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
JP6454281B2 (ja) 2012-11-05 2019-01-16 タカラ バイオ ユーエスエー, インコーポレイテッド バーコード化する核酸
US9128861B2 (en) 2013-01-17 2015-09-08 Personalis, Inc. Methods and systems for genetic analysis
CN105408496A (zh) 2013-03-15 2016-03-16 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
WO2014210225A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
EP3965111A1 (en) 2013-08-30 2022-03-09 Personalis, Inc. Methods and systems for genomic analysis
GB2535066A (en) 2013-10-03 2016-08-10 Personalis Inc Methods for analyzing genotypes
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
KR102379877B1 (ko) 2013-12-11 2022-03-30 아큐라젠 홀딩스 리미티드 희귀 서열 변이를 검출하기 위한 조성물 및 방법
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CN111534580A (zh) * 2013-12-28 2020-08-14 夸登特健康公司 用于检测遗传变异的方法和系统
MX2016010100A (es) 2014-02-05 2016-10-07 Fraunhofer Ges Forschung Secuenciacion libre de error de acido desoxirribonucleico (adn).
US20150298091A1 (en) * 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
WO2015175705A1 (en) 2014-05-13 2015-11-19 Board Of Regents, The University Of Texas System Gene mutations and copy number alterations of egfr, kras and met
GB201409282D0 (en) 2014-05-23 2014-07-09 Univ Sydney Tech Sequencing process
EP3169799B1 (en) * 2014-07-15 2019-09-04 Qiagen Sciences, LLC Semi-random barcodes for nucleic acid analysis
CN107075730A (zh) * 2014-09-12 2017-08-18 利兰·斯坦福青年大学托管委员会 循环核酸的鉴定及用途
EP4026913A1 (en) 2014-10-30 2022-07-13 Personalis, Inc. Methods for using mosaicism in nucleic acids sampled distal to their origin
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
ES2923602T3 (es) 2014-12-31 2022-09-28 Guardant Health Inc Detección y tratamiento de enfermedades que muestran heterogeneidad celular de enfermedad y sistemas y métodos para comunicar los resultados de las pruebas
US10421993B2 (en) 2015-02-11 2019-09-24 Paragon Genomics, Inc. Methods and compositions for reducing non-specific amplification products
EP3901281B1 (en) 2015-04-10 2022-11-23 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10844428B2 (en) * 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US10988802B2 (en) 2015-05-22 2021-04-27 Sigma-Aldrich Co. Llc Methods for next generation genome walking and related compositions and kits
JP6698708B2 (ja) * 2015-06-09 2020-05-27 ライフ テクノロジーズ コーポレーション 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体
GB201515557D0 (en) 2015-09-02 2015-10-14 14M Genomics Ltd Method of sequencing
WO2017044886A1 (en) * 2015-09-09 2017-03-16 uBiome, Inc. Method and system for microbiome-derived diagnostics and therapeutics for bacterial vaginosis
EP3359693A4 (en) 2015-10-09 2019-03-06 Accuragen Holdings Limited METHODS AND COMPOSITIONS FOR ENRICHMENT OF AMPLIFICATION PRODUCTS
KR20180113973A (ko) 2015-11-11 2018-10-17 레졸루션 바이오사이언스, 인크. Dna 라이브러리의 고효율 작제
AU2016366231B2 (en) * 2015-12-08 2022-12-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
WO2017127741A1 (en) * 2016-01-22 2017-07-27 Grail, Inc. Methods and systems for high fidelity sequencing
WO2017132438A1 (en) * 2016-01-29 2017-08-03 The Johns Hopkins University Bottleneck sequencing
EP3411505A4 (en) 2016-02-02 2020-01-15 Guardant Health, Inc. DETECTION AND DIAGNOSIS OF CANCER EVOLUTION
EP4282974A3 (en) 2016-03-28 2024-03-13 Ncan Genomics, Inc. Linked duplex target capture
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
SG11201808775PA (en) 2016-04-07 2018-11-29 Univ Leland Stanford Junior Noninvasive diagnostics by sequencing 5-hydroxymethylated cell-free dna
US11384382B2 (en) 2016-04-14 2022-07-12 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
WO2017181146A1 (en) 2016-04-14 2017-10-19 Guardant Health, Inc. Methods for early detection of cancer
CA3022863A1 (en) 2016-05-02 2017-11-09 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding of molecular recognition events
CN109511265B (zh) * 2016-05-16 2023-07-14 安可济控股有限公司 通过链鉴定改进测序的方法
US11299783B2 (en) 2016-05-27 2022-04-12 Personalis, Inc. Methods and systems for genetic analysis
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
CA3024984C (en) 2016-06-30 2021-12-07 Grail, Inc. Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
EP3485033B1 (en) 2016-07-12 2022-09-28 Qiagen Sciences, LLC Single end duplex dna sequencing
CN109804086B (zh) * 2016-08-10 2023-06-13 格里尔公司 制备双标签dna库用于亚硫酸盐转化定序的方法
SG11201901296TA (en) 2016-08-15 2019-03-28 Accuragen Holdings Ltd Compositions and methods for detecting rare sequence variants
JP7217224B2 (ja) 2016-08-25 2023-02-02 レゾリューション バイオサイエンス, インコーポレイテッド Dna試料中のゲノムコピー変化の検出方法
US11959125B2 (en) * 2016-09-15 2024-04-16 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
CA3037366A1 (en) 2016-09-29 2018-04-05 Myriad Women's Health, Inc. Noninvasive prenatal screening using dynamic iterative depth optimization
JP7048609B2 (ja) 2016-12-09 2022-04-05 ボリアル ジェノミクス, インコーポレイテッド 連結型ライゲーション
WO2018114706A1 (en) 2016-12-20 2018-06-28 F. Hoffmann-La Roche Ag Single stranded circular dna libraries for circular consensus sequencing
JP7300989B2 (ja) 2016-12-22 2023-06-30 ガーダント ヘルス, インコーポレイテッド 核酸分子を解析するための方法およびシステム
US10982351B2 (en) 2016-12-23 2021-04-20 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
EP3562961A4 (en) * 2016-12-28 2021-01-06 Quest Diagnostics Investments LLC COMPOSITIONS AND METHODS OF DETECTION OF CIRCULATING TUMOR DNA
US10100358B2 (en) 2017-01-10 2018-10-16 Paragon Genomics, Inc. Methods and compositions for reducing redundant molecular barcodes created in primer extension reactions
SG11201906428SA (en) 2017-01-18 2019-08-27 Illumina Inc Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
US11352662B2 (en) * 2017-01-20 2022-06-07 Sequenom, Inc. Sequence adapter manufacture and use
WO2018144217A1 (en) * 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
WO2018144216A1 (en) 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
WO2018144159A1 (en) * 2017-01-31 2018-08-09 Counsyl, Inc. Capture probes using positive and negative strands for duplex sequencing
WO2018175997A1 (en) * 2017-03-23 2018-09-27 University Of Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
EP3602359A4 (en) 2017-03-24 2021-01-06 Myriad Women's Health, Inc. NUMBER OF COPIES VARIANT CALLER
WO2018183918A1 (en) * 2017-03-30 2018-10-04 Grail, Inc. Enhanced ligation in sequencing library preparation
WO2018183897A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Higher target capture efficiency using probe extension
US11584958B2 (en) 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
SG11201909697TA (en) 2017-05-01 2019-11-28 Illumina Inc Optimal index sequences for multiplex massively parallel sequencing
SG10202113017YA (en) 2017-05-08 2021-12-30 Illumina Inc Universal short adapters for indexing of polynucleotide samples
ES2864101T3 (es) 2017-06-01 2021-10-13 Siemens Healthcare Gmbh Método y sistema para la secuenciación de ácidos nucleicos
GB2578038B (en) 2017-06-16 2022-11-23 Life Technologies Corp Control nucleic acids, and compositions, kits, and uses thereof
US11505826B2 (en) 2017-07-12 2022-11-22 Agilent Technologies, Inc. Sequencing method for genomic rearrangement detection
JP6998404B2 (ja) * 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 標的ヌクレオチド配列の富化及び決定方法
CN111315884B (zh) * 2017-09-08 2024-03-29 普梭梅根公司 测序文库的归一化
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
WO2019071219A1 (en) 2017-10-06 2019-04-11 Grail, Inc. SPECIFIC SITE NOISE MODEL FOR TARGETED SEQUENCING
AU2018358057B2 (en) 2017-10-31 2023-03-02 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
EP3704265A4 (en) 2017-11-03 2021-09-29 Guardant Health, Inc. CORRECTION OF SEQUENCE ERRORS INDUCED BY DESAMINATION
CN107604046B (zh) * 2017-11-03 2021-08-24 上海交通大学 用于微量dna超低频突变检测的双分子自校验文库制备及杂交捕获的二代测序方法
KR20230028569A (ko) 2017-11-06 2023-02-28 일루미나, 인코포레이티드 핵산 색인 기술
SG11202003885UA (en) 2017-11-08 2020-05-28 Twinstrand Biosciences Inc Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
AU2018375302A1 (en) 2017-11-28 2020-06-11 Grail, Llc Models for targeted sequencing
JP7013490B2 (ja) * 2017-11-30 2022-02-15 イルミナ インコーポレイテッド 配列バリアントコールのためのバリデーションの方法及びシステム
US20190218606A1 (en) * 2018-01-18 2019-07-18 Omigen (hangzhou) Inc. Methods of reducing errors in deep sequencing
WO2019160998A1 (en) * 2018-02-13 2019-08-22 Twinstrand Biosciences, Inc. Methods and reagents for detecting and assessing genotoxicity
CN108359723B (zh) * 2018-02-23 2021-06-08 奥明(杭州)基因科技有限公司 一种降低深度测序错误的方法
EP3765063A4 (en) * 2018-03-15 2021-12-15 Twinstrand Biosciences, Inc. METHODS AND REAGENTS FOR ENRICHMENT NUCLEIC ACID MATERIAL FOR SEQUENCING APPLICATIONS AND OTHER NUCLEIC ACID MATERIAL INTERROGATIONS
GB201804642D0 (en) 2018-03-22 2018-05-09 Inivata Ltd Methods of labelling nucleic acids
GB201804641D0 (en) * 2018-03-22 2018-05-09 Inivata Ltd Methods of sequencing nucleic acids and error correction of sequence reads
EP3768853A4 (en) 2018-03-23 2021-04-28 Board Of Regents The University Of Texas System EFFICIENT SEQUENCING OF DSDNA WITH EXTREMELY LOW ERROR RATE
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
EP4353836A2 (en) 2018-04-27 2024-04-17 Kao Corporation Highly accurate sequencing method
JP2021524736A (ja) * 2018-05-16 2021-09-16 ツインストランド・バイオサイエンシズ・インコーポレイテッドTwinstrand Biosciences, Inc. 核酸混合物および混合細胞集団を解析するための方法および試薬ならびに関連用途
US10801064B2 (en) 2018-05-31 2020-10-13 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
US11814750B2 (en) 2018-05-31 2023-11-14 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
JP2021527436A (ja) 2018-06-22 2021-10-14 ブルースター ジェノミクス, インコーポレイテッド 起源組織を割り当てるための無細胞核酸サンプルのヒドロキシメチル化分析および関連する使用方法
SG11202100141SA (en) 2018-07-12 2021-02-25 Twinstrand Biosciences Inc Methods and reagents for characterizing genomic editing, clonal expansion, and associated applications
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020061380A1 (en) 2018-09-19 2020-03-26 Bluestar Genomics, Inc. Cell-free dna hydroxymethylation profiles in the evaluation of pancreatic lesions
CA3111887A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
US10696994B2 (en) 2018-09-28 2020-06-30 Bioo Scientific Corporation Size selection of RNA using poly(A) polymerase
US11104941B2 (en) 2018-09-28 2021-08-31 Bioo Scientific Corporation 5′ adapter comprising an internal 5′-5′ linkage
WO2020081743A1 (en) * 2018-10-16 2020-04-23 Twinstrand Biosciences, Inc. Methods and reagents for efficient genotyping of large numbers of samples via pooling
WO2020123319A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
EP3884071A4 (en) 2019-01-03 2022-04-06 Boreal Genomics, Inc. LINKED TARGET CAPTURE
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
KR20210148122A (ko) * 2019-02-25 2021-12-07 트위스트 바이오사이언스 코포레이션 차세대 시퀀싱을 위한 조성물 및 방법
US11952613B2 (en) * 2019-03-11 2024-04-09 Phillip N. Gray Methods and reagents for enhanced next generation sequencing library conversion and incorporation of molecular barcodes into targeted and random nucleic acid sequences
JP2022530966A (ja) 2019-04-30 2022-07-05 エンコディア, インコーポレイテッド 分析物を調製するための方法および関連キット
EP3983558A4 (en) * 2019-06-12 2023-06-28 Ultima Genomics, Inc. Methods for accurate base calling using molecular barcodes
US20220301654A1 (en) 2019-08-28 2022-09-22 Grail, Llc Systems and methods for predicting and monitoring treatment response from cell-free nucleic acids
WO2021055431A1 (en) * 2019-09-16 2021-03-25 Cornell University Human mitochondrial dna sequencing by targeted amplification of multiplex probes (mtdna-stamp)
CA3151538A1 (en) 2019-09-30 2021-04-08 Stefanie Ann Ward Mortimer Compositions and methods for analyzing cell-free dna in methylation partitioning assays
GB202318607D0 (en) 2019-11-06 2024-01-17 Univ Leland Stanford Junior Methods and systems for analyzing nucleic acid molecules
EP4083278A4 (en) 2019-11-15 2024-02-28 Kao Corp METHOD FOR CREATING A SEQUENCING LIBRARY
SG11202106899SA (en) 2019-12-23 2021-09-29 10X Genomics Inc Methods for spatial analysis using rna-templated ligation
AU2021208466A1 (en) 2020-01-13 2022-08-11 Fluent Biosciences Inc. Single cell sequencing
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4114966A1 (en) 2020-03-03 2023-01-11 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
US11359238B2 (en) 2020-03-06 2022-06-14 Singular Genomics Systems, Inc. Linked paired strand sequencing
CN111254198A (zh) * 2020-03-09 2020-06-09 苏州宏元生物科技有限公司 一种尿路上皮癌诊断和预后评估试剂盒
CA3175931A1 (en) 2020-03-16 2021-09-23 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US10941453B1 (en) 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (en) 2020-06-10 2023-04-19 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
CA3200517A1 (en) * 2020-11-03 2022-05-12 Fluent Biosciences Inc. Systems and methods for making sequencing libraries
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022109389A1 (en) * 2020-11-20 2022-05-27 Camena Bioscience Limited Geometric synthesis methods and compositions for double-stranded nucleic acid sequencing
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20220336052A1 (en) * 2021-04-19 2022-10-20 University Of Utah Research Foundation Systems and methods for facilitating rapid genome sequence analysis
US11783912B2 (en) 2021-05-05 2023-10-10 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for analyzing nucleic acid molecules
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11427855B1 (en) 2021-06-17 2022-08-30 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
US11873533B2 (en) * 2021-09-06 2024-01-16 Lucence Life Sciences Pte. Ltd. Method of detecting and quantifying geonomic and gene expression alterations using RNA
WO2023141347A2 (en) * 2022-01-24 2023-07-27 Gusto Global, Llc Single-loci and multi-loci targeted single point amplicon fragment sequencing
WO2023175021A1 (en) * 2022-03-15 2023-09-21 Illumina, Inc. Methods of preparing loop fork libraries
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
GB202209189D0 (en) 2022-06-22 2022-08-10 Broken String Biosciences Ltd Methods and compositions for nucleic acid sequencing

Family Cites Families (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5308751A (en) * 1992-03-23 1994-05-03 General Atomics Method for sequencing double-stranded DNA
ES2563643T3 (es) 1997-04-01 2016-03-15 Illumina Cambridge Limited Método de secuenciación de ácido nucleico
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
EP1124990B1 (en) 1998-10-27 2006-01-18 Affymetrix, Inc. Complexity management and analysis of genomic dna
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
CA2360342A1 (en) 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
US7300751B2 (en) 2000-06-30 2007-11-27 Syngenta Participations Ag Method for identification of genetic markers
US6632611B2 (en) 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
CN100494395C (zh) * 2001-10-15 2009-06-03 生物芯片技术有限公司 通过同时探查和酶介导检测的多态性基因座多重分析
US7406385B2 (en) 2001-10-25 2008-07-29 Applera Corporation System and method for consensus-calling with per-base quality values for sample assemblies
US7459273B2 (en) 2002-10-04 2008-12-02 Affymetrix, Inc. Methods for genotyping selected polymorphism
US7452699B2 (en) 2003-01-15 2008-11-18 Dana-Farber Cancer Institute, Inc. Amplification of DNA in a hairpin structure, and applications
WO2004081183A2 (en) 2003-03-07 2004-09-23 Rubicon Genomics, Inc. In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna
EP1709203A2 (en) 2004-01-23 2006-10-11 Lingvitae AS Improving polynucleotide ligation reactions
CA2579150C (en) 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US8796441B2 (en) * 2005-04-13 2014-08-05 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Human sweet and umami taste receptor variants
EP1910562B1 (en) 2005-06-23 2010-12-08 Keygene N.V. Strategies for high throughput identification and detection of polymorphisms
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
CA2910861C (en) 2005-09-29 2018-08-07 Michael Josephus Theresia Van Eijk High throughput screening of mutagenized populations
GB0522310D0 (en) * 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US20070172839A1 (en) 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
EP2002017B1 (en) 2006-04-04 2015-06-10 Keygene N.V. High throughput detection of molecular markers based on restriction fragments
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2009036525A2 (en) 2007-09-21 2009-03-26 Katholieke Universiteit Leuven Tools and methods for genetic tests using next generation sequencing
WO2009082488A2 (en) * 2007-12-24 2009-07-02 Bergenbio As Methods for creating and identifying functional rna interference elements
CN104862383B (zh) * 2008-03-28 2019-05-28 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
US8029993B2 (en) 2008-04-30 2011-10-04 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2010056728A1 (en) * 2008-11-11 2010-05-20 Helicos Biosciences Corporation Nucleic acid encoding for multiplex analysis
AU2010209528B2 (en) 2009-01-30 2015-10-01 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US20120165202A1 (en) 2009-04-30 2012-06-28 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
EP2248914A1 (en) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The use of class IIB restriction endonucleases in 2nd generation sequencing applications
EP2467479B1 (en) 2009-08-20 2016-01-06 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP3425062B1 (en) 2010-06-09 2023-08-02 Keygene N.V. Combinatorial sequence barcodes for high throughput screening
US9080169B2 (en) * 2010-06-11 2015-07-14 Brandeis University Methods for the development of vaccines based on oligosaccharide-oligonucleotide conjugates
WO2012012037A1 (en) 2010-07-19 2012-01-26 New England Biolabs, Inc. Oligonucleotide adaptors: compositions and methods of use
US20120244525A1 (en) 2010-07-19 2012-09-27 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
ES2523140T3 (es) 2010-09-21 2014-11-21 Population Genetics Technologies Ltd. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
WO2012042374A2 (en) 2010-10-01 2012-04-05 Anssi Jussi Nikolai Taipale Method of determining number or concentration of molecules
EP2635679B1 (en) * 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
DK2652155T3 (en) 2010-12-16 2017-02-13 Gigagen Inc Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
WO2012129242A2 (en) 2011-03-23 2012-09-27 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
EP3907297A1 (en) 2011-04-15 2021-11-10 The Johns Hopkins University Safe sequencing system
EP2980226A1 (en) 2011-07-08 2016-02-03 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
EP4219740A3 (en) * 2011-09-06 2023-08-16 Gen-Probe Incorporated Closed nucleic acid structures
EP2753715A4 (en) 2011-09-09 2015-05-20 Univ Leland Stanford Junior METHODS FOR OBTAINING A SEQUENCE
US9528107B2 (en) 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
PL2814959T3 (pl) 2012-02-17 2018-07-31 Fred Hutchinson Cancer Research Center Kompozycje i sposoby do dokładnej identyfikacji mutacji
ES2776673T3 (es) 2012-02-27 2020-07-31 Univ North Carolina Chapel Hill Métodos y usos para etiquetas moleculares
EP2825675B1 (en) 2012-03-13 2017-12-27 Patel, Abhijit Ajit Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
HUE051845T2 (hu) * 2012-03-20 2021-03-29 Univ Washington Through Its Center For Commercialization Módszerek a tömegesen párhuzamos DNS-szekvenálás hibaarányának csökkentésére duplex konszenzus szekvenálással
WO2013181170A1 (en) * 2012-05-31 2013-12-05 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
EP2893040B1 (en) 2012-09-04 2019-01-02 Guardant Health, Inc. Methods to detect rare mutations and copy number variation
CN111534580A (zh) 2013-12-28 2020-08-14 夸登特健康公司 用于检测遗传变异的方法和系统
CN107075730A (zh) 2014-09-12 2017-08-18 利兰·斯坦福青年大学托管委员会 循环核酸的鉴定及用途
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US11584958B2 (en) 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification

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