NZ729729A - Production of fully processed and functional factor x in a furin-secreting mammalian expression system - Google Patents

Production of fully processed and functional factor x in a furin-secreting mammalian expression system

Info

Publication number
NZ729729A
NZ729729A NZ729729A NZ72972915A NZ729729A NZ 729729 A NZ729729 A NZ 729729A NZ 729729 A NZ729729 A NZ 729729A NZ 72972915 A NZ72972915 A NZ 72972915A NZ 729729 A NZ729729 A NZ 729729A
Authority
NZ
New Zealand
Prior art keywords
furin
production
expression system
mammalian expression
fully processed
Prior art date
Application number
NZ729729A
Other languages
English (en)
Inventor
Michael Dockal
Ernst Böhm
Franziska Horling
Jadranka Koehn
Original Assignee
Takeda Pharmaceuticals Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Pharmaceuticals Co filed Critical Takeda Pharmaceuticals Co
Publication of NZ729729A publication Critical patent/NZ729729A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A61K38/4846—Factor VII (3.4.21.21); Factor IX (3.4.21.22); Factor Xa (3.4.21.6); Factor XI (3.4.21.27); Factor XII (3.4.21.38)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6454—Dibasic site splicing serine proteases, e.g. kexin (3.4.21.61); furin (3.4.21.75) and other proprotein convertases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21075—Furin (3.4.21.75)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
NZ729729A 2014-08-12 2015-08-12 Production of fully processed and functional factor x in a furin-secreting mammalian expression system NZ729729A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462036438P 2014-08-12 2014-08-12
PCT/US2015/044883 WO2016025615A1 (en) 2014-08-12 2015-08-12 Production of fully processed and functional factor x in a furin-secreting mammalian expression system

Publications (1)

Publication Number Publication Date
NZ729729A true NZ729729A (en) 2023-07-28

Family

ID=54035298

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ729729A NZ729729A (en) 2014-08-12 2015-08-12 Production of fully processed and functional factor x in a furin-secreting mammalian expression system

Country Status (14)

Country Link
US (1) US9873892B2 (cg-RX-API-DMAC7.html)
EP (1) EP3180427B1 (cg-RX-API-DMAC7.html)
JP (1) JP6793114B2 (cg-RX-API-DMAC7.html)
CN (1) CN107002041B (cg-RX-API-DMAC7.html)
AR (1) AR102048A1 (cg-RX-API-DMAC7.html)
AU (1) AU2015301726B2 (cg-RX-API-DMAC7.html)
CA (1) CA2958056C (cg-RX-API-DMAC7.html)
EA (1) EA035444B1 (cg-RX-API-DMAC7.html)
ES (1) ES2742724T3 (cg-RX-API-DMAC7.html)
IL (1) IL250530B (cg-RX-API-DMAC7.html)
MX (1) MX377236B (cg-RX-API-DMAC7.html)
NZ (1) NZ729729A (cg-RX-API-DMAC7.html)
TW (1) TWI666319B (cg-RX-API-DMAC7.html)
WO (1) WO2016025615A1 (cg-RX-API-DMAC7.html)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8241623B1 (en) 2009-02-09 2012-08-14 David Bermudes Protease sensitivity expression system
US11299533B2 (en) 2017-06-23 2022-04-12 Takeda Pharmaceutical Company Limited Purification of factor VIII subspecies
WO2020257942A1 (en) * 2019-06-28 2020-12-30 Peel Sean A F A method for increasing yields and the specific activity of certain recombinant proteins in mammalian cells by co-expressing full-length furin
GB202018320D0 (en) * 2020-11-20 2021-01-06 Univ Newcastle Methods of producing recombinant complement proteins
WO2023141754A1 (zh) * 2022-01-25 2023-08-03 青岛万明赛伯药业有限公司 proNGF突变体及其用途

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0574402B2 (en) 1990-11-26 2012-08-01 Genetics Institute, LLC Expression of pace in host cells and methods of use thereof
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
JPH09295945A (ja) * 1996-04-30 1997-11-18 Hoechst Yakuhin Kogyo Kk 成熟型骨誘導因子の製造方法
US5876923A (en) 1996-07-26 1999-03-02 Arch Development Corporation Herpes simplex virus ICP4 as an inhibitor of apoptosis
AU784195B2 (en) 1999-11-12 2006-02-16 Baxter Biotech Technology S.A.R.L. Reduced side-effect hemoglobin compositions
US6596526B1 (en) * 2000-06-09 2003-07-22 Baxter Aktiengesellschaft Furin polypeptides with improved characteristics
US7223390B2 (en) * 2003-05-09 2007-05-29 Research Development Foundation Insertion of furin protease cleavage sites in membrane proteins and uses thereof
EP1969127B8 (en) * 2005-12-21 2017-12-13 Aptevo BioTherapeutics LLC Method of producing biologically active vitamin k dependent proteins by recombinant methods
KR101156263B1 (ko) 2007-05-18 2012-06-13 박스터 헬쓰케어 에스.에이. 폰 빌리브란트 인자 프로펩티드로부터 성숙한 폰 빌리브란트 인자를 제조하는 방법
WO2009088713A1 (en) * 2007-12-31 2009-07-16 Baxter International Inc. Substantially animal protein-free recombinant furin and methods for producing same
JP5843357B2 (ja) 2009-02-03 2016-01-13 アムニクス オペレーティング インコーポレイテッド 延長組換えポリペプチドおよび延長組換えポリペプチドを含む組成物
CA2837948C (en) 2011-06-02 2018-08-21 Baxter International Inc. Formulations of recombinant furin

Also Published As

Publication number Publication date
CN107002041B (zh) 2021-06-29
AU2015301726A1 (en) 2017-03-16
EA035444B1 (ru) 2020-06-17
US9873892B2 (en) 2018-01-23
JP2017524366A (ja) 2017-08-31
MX377236B (es) 2025-03-07
EP3180427A1 (en) 2017-06-21
CA2958056A1 (en) 2016-02-18
MX2017001931A (es) 2017-06-30
CN107002041A (zh) 2017-08-01
IL250530B (en) 2020-03-31
AR102048A1 (es) 2017-02-01
EP3180427B1 (en) 2019-05-22
IL250530A0 (en) 2017-03-30
WO2016025615A1 (en) 2016-02-18
TW201625791A (zh) 2016-07-16
US20160046957A1 (en) 2016-02-18
TWI666319B (zh) 2019-07-21
CA2958056C (en) 2023-07-04
EA201790360A1 (ru) 2017-06-30
AU2015301726B2 (en) 2021-10-21
ES2742724T3 (es) 2020-02-17
JP6793114B2 (ja) 2020-12-09

Similar Documents

Publication Publication Date Title
ZA202310779B (en) Compositions and methods for enhanced gene expression
IL249828A0 (en) Limbal stem cells of cultured mammals, methods of their creation and uses thereof
MX2025004237A (es) Metodo de diferenciacion mejorada
MX2020000369A (es) Metodos biologicos para preparar terpenos.
MX364690B (es) Proceso de elaboracion escalable para producir vectores lentivirales recombinantes en un sistema de cultivo celular en suspension libre de suero.
WO2019118829A3 (en) Sialyltransferases and uses thereof
MX2015012168A (es) Expansión de celulas madre adultas in vitro.
SG10201809452RA (en) Cell culture compositions with antioxidants and methods for polypeptide production
WO2017083858A3 (en) Microbial production of nicotinamide riboside
NZ729729A (en) Production of fully processed and functional factor x in a furin-secreting mammalian expression system
PH12017502240A1 (en) Recombinant microorganism for improved production of alanine
MX2016003737A (es) Microorganismo recombinante para la mejora de produccion de productos quimicos finos.
PH12016501094A1 (en) Recombinant microorganism for improved production of fine chemicals
MX366112B (es) Medio de cultivo celular y metodos de produccion de anticuerpos.
MY185385A (en) Control systems and methods suitable for use with power production systems and methods
PH12018500150A1 (en) Microorganisms for producing putrescine or ornithine and process for producing putrescine or ornithine using them
MX2022003016A (es) Metodo de produccion con iones de cobre controlados.
PH12019500264A1 (en) Lactobacillus salivarius cjls1511, animal feed additive composition comprising same bacterium or dead cells thereof, and method for producing same dead cells
MX2021001981A (es) Metodos para preparar un poloxamero para usar en medio de cultivo celular.
MY184391A (en) Microorganism having improved intracellular energy level and method for producing l-amino acid using same
PH12018500069A1 (en) Novel lactobacillus sp. microorganisms, and composition for animal feed comprising same
MY174840A (en) Microorganism producing o-acetyl-homoserine, and method for producing o-acetyl-homoserine by using same
ZA202004214B (en) Method for producing 1,3-propylene glycol by means of fermentation of recombinant microorganism
MY177808A (en) A process of production and extra-cellular secretion of lipids
GB2535968A (en) Method

Legal Events

Date Code Title Description
ASS Change of ownership

Owner name: TAKEDA PHARMACEUTICAL COMPANY, JP

Effective date: 20210621

PSEA Patent sealed
RENW Renewal (renewal fees accepted)

Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 12 AUG 2025 BY ANAQUA SERVICES

Effective date: 20240723

RENW Renewal (renewal fees accepted)

Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 12 AUG 2026 BY ANAQUA SERVICES

Effective date: 20250723