MX377236B - Produccion de un factor x totalmente procesado y funcional en un sistema de expresion en mamifero para secrecion de furina. - Google Patents

Produccion de un factor x totalmente procesado y funcional en un sistema de expresion en mamifero para secrecion de furina.

Info

Publication number
MX377236B
MX377236B MX2017001931A MX2017001931A MX377236B MX 377236 B MX377236 B MX 377236B MX 2017001931 A MX2017001931 A MX 2017001931A MX 2017001931 A MX2017001931 A MX 2017001931A MX 377236 B MX377236 B MX 377236B
Authority
MX
Mexico
Prior art keywords
furin
factor
production
secretion
expression system
Prior art date
Application number
MX2017001931A
Other languages
English (en)
Other versions
MX2017001931A (es
Inventor
Ernst Böhm
Franziska Horling
Jadranka Koehn
Michael Dockal
Original Assignee
Takeda Pharmaceuticals Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Pharmaceuticals Co filed Critical Takeda Pharmaceuticals Co
Publication of MX2017001931A publication Critical patent/MX2017001931A/es
Publication of MX377236B publication Critical patent/MX377236B/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A61K38/4846—Factor VII (3.4.21.21); Factor IX (3.4.21.22); Factor Xa (3.4.21.6); Factor XI (3.4.21.27); Factor XII (3.4.21.38)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6454—Dibasic site splicing serine proteases, e.g. kexin (3.4.21.61); furin (3.4.21.75) and other proprotein convertases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21075—Furin (3.4.21.75)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invención se refiere a métodos para la producción de un Factor X maduro totalmente procesado en un sistema de expresión que produce una cantidad controlada de furina entre 50 U/mL y 300 U/mL de un sobrenadante de cultivo. También se describen células transformadas, sistemas de expresión, y vectores de expresión para la expresión de furina y el Factor X.
MX2017001931A 2014-08-12 2015-08-12 Produccion de un factor x totalmente procesado y funcional en un sistema de expresion en mamifero para secrecion de furina. MX377236B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462036438P 2014-08-12 2014-08-12
PCT/US2015/044883 WO2016025615A1 (en) 2014-08-12 2015-08-12 Production of fully processed and functional factor x in a furin-secreting mammalian expression system

Publications (2)

Publication Number Publication Date
MX2017001931A MX2017001931A (es) 2017-06-30
MX377236B true MX377236B (es) 2025-03-07

Family

ID=54035298

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017001931A MX377236B (es) 2014-08-12 2015-08-12 Produccion de un factor x totalmente procesado y funcional en un sistema de expresion en mamifero para secrecion de furina.

Country Status (14)

Country Link
US (1) US9873892B2 (es)
EP (1) EP3180427B1 (es)
JP (1) JP6793114B2 (es)
CN (1) CN107002041B (es)
AR (1) AR102048A1 (es)
AU (1) AU2015301726B2 (es)
CA (1) CA2958056C (es)
EA (1) EA035444B1 (es)
ES (1) ES2742724T3 (es)
IL (1) IL250530B (es)
MX (1) MX377236B (es)
NZ (1) NZ729729A (es)
TW (1) TWI666319B (es)
WO (1) WO2016025615A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8241623B1 (en) 2009-02-09 2012-08-14 David Bermudes Protease sensitivity expression system
CN111183151B (zh) 2017-06-23 2024-06-07 武田药品工业株式会社 因子viii亚种的纯化
WO2020257942A1 (en) * 2019-06-28 2020-12-30 Peel Sean A F A method for increasing yields and the specific activity of certain recombinant proteins in mammalian cells by co-expressing full-length furin
GB202018320D0 (en) * 2020-11-20 2021-01-06 Univ Newcastle Methods of producing recombinant complement proteins
CN115298204B (zh) * 2022-01-25 2023-04-25 青岛万明赛伯药业有限公司 proNGF突变体及其用途

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4236698B2 (ja) * 1990-11-26 2009-03-11 ジェネティックス インスティチュート，リミテッド ライアビリティ カンパニー 宿主細胞でのｐａｃｅの発現およびその使用法
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
JPH09295945A (ja) * 1996-04-30 1997-11-18 Hoechst Yakuhin Kogyo Kk 成熟型骨誘導因子の製造方法
US5876923A (en) 1996-07-26 1999-03-02 Arch Development Corporation Herpes simplex virus ICP4 as an inhibitor of apoptosis
EP1233986A4 (en) 1999-11-12 2003-01-02 Baxter Biotech Tech Sarl HEMOGLOBIN COMPOSITIONS WITH REDUCED SIDE EFFECTS
US6596526B1 (en) * 2000-06-09 2003-07-22 Baxter Aktiengesellschaft Furin polypeptides with improved characteristics
WO2004101598A2 (en) * 2003-05-09 2004-11-25 Research Development Foundation Insertion of furin protease cleavage sites in membrane proteins and uses thereof
DK1969127T4 (en) * 2005-12-21 2017-10-16 Cnj Holdings Inc Process for the preparation of biologically active vitamin K-dependent proteins by recombinant methods
ES2539284T3 (es) 2007-05-18 2015-06-29 Baxter International Inc. Procedimiento de producción de VWF maduro a partir del propéptido de VWF
MX2010007344A (es) * 2007-12-31 2010-11-09 Baxter Int Furina recombinante substancialmente libre de proteina animal y metodos para la produccion de la misma.
PL2393828T3 (pl) 2009-02-03 2017-06-30 Amunix Operating Inc. Wydłużone rekombinowane polipeptydy i zawierające je kompozycje
SG10201604488TA (en) * 2011-06-02 2016-07-28 Baxter Int Formulations Of Recombinant Furin

Also Published As

Publication number Publication date
IL250530A0 (en) 2017-03-30
AU2015301726B2 (en) 2021-10-21
JP2017524366A (ja) 2017-08-31
EA035444B1 (ru) 2020-06-17
CA2958056C (en) 2023-07-04
US20160046957A1 (en) 2016-02-18
IL250530B (en) 2020-03-31
US9873892B2 (en) 2018-01-23
AR102048A1 (es) 2017-02-01
TW201625791A (zh) 2016-07-16
CN107002041A (zh) 2017-08-01
WO2016025615A1 (en) 2016-02-18
NZ729729A (en) 2023-07-28
CN107002041B (zh) 2021-06-29
ES2742724T3 (es) 2020-02-17
EP3180427B1 (en) 2019-05-22
MX2017001931A (es) 2017-06-30
CA2958056A1 (en) 2016-02-18
TWI666319B (zh) 2019-07-21
JP6793114B2 (ja) 2020-12-09
EA201790360A1 (ru) 2017-06-30
EP3180427A1 (en) 2017-06-21
AU2015301726A1 (en) 2017-03-16

Similar Documents

Publication Publication Date Title
MX2025004237A (es) Metodo de diferenciacion mejorada
IL249828A0 (en) Limbal stem cells of cultured mammals, methods of their creation and uses thereof
SA518392270B1 (ar) إنتاج ملاط ثلجي طبي وأنظمة توصيل وطرق
MX2021008143A (es) Metodos para cosechar cultivos de celulas de mamifero.
PH12016502162A1 (en) Multiplex gene editing in swine
NZ733055A (en) Anti-c10orf54 antibodies and uses thereof
MX390195B (es) Vectores para uso en un sistema de coexpresion inducible.
MY173953A (en) Recombinant host cells for the production of malonate
MX2021001859A (es) Medio de cultivo celular libre de suero.
MX363690B (es) Expansión de celulas madre adultas in vitro.
CL2017001227A1 (es) Ganado lechero híbrido y sistemas para maximizar la ventaja híbrida.
MX377236B (es) Produccion de un factor x totalmente procesado y funcional en un sistema de expresion en mamifero para secrecion de furina.
EA201791275A1 (ru) Способы получения ctp-модифицированных полипептидов длительного действия
MX2016003737A (es) Microorganismo recombinante para la mejora de produccion de productos quimicos finos.
UA110352C2 (en) Control system of electrostatic separation
PH12016501094A1 (en) Recombinant microorganism for improved production of fine chemicals
MX2015011781A (es) Medio de cultivo celular y metodos de produccion de anticuerpos.
PH12016501689A1 (en) Tatk-cdkl5 fusion proteins, compositions, formulations, and use thereof
MX2018016417A (es) Proteinas de fusion tatk-cdkl5, composiciones, formulaciones y uso de las mismas.
MY184391A (en) Microorganism having improved intracellular energy level and method for producing l-amino acid using same
MX390879B (es) Metodo de produccion con iones de cobre controlados.
MX2021009154A (es) Selectividad eficaz de proteinas recombinantes.
GB2535968A (en) Method
EA201991474A1 (ru) Способы получения фитоина
EP3463394A4 (en) NOVEL CELL CULTURE METHOD, CELL CULTURE SYSTEM AND USES THEREOF

Legal Events

Date Code Title Description
FG Grant or registration
PD Change of proprietorship

Owner name: TAKEDA PHARMACEUTICAL COMPANY LIMITED