NO975748L - Kromosomal ekspresjon av heterologe gener i bakterielle celler - Google Patents

Kromosomal ekspresjon av heterologe gener i bakterielle celler

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Publication number
NO975748L
NO975748L NO975748A NO975748A NO975748L NO 975748 L NO975748 L NO 975748L NO 975748 A NO975748 A NO 975748A NO 975748 A NO975748 A NO 975748A NO 975748 L NO975748 L NO 975748L
Authority
NO
Norway
Prior art keywords
interest
chromosomal
dna
host cell
gene encoding
Prior art date
Application number
NO975748A
Other languages
English (en)
Other versions
NO975748D0 (no
Inventor
Desmond Mascarenhas
Pamela Olson
Original Assignee
Celtrix Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celtrix Pharma filed Critical Celtrix Pharma
Publication of NO975748D0 publication Critical patent/NO975748D0/no
Publication of NO975748L publication Critical patent/NO975748L/no

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4743—Insulin-like growth factor binding protein
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/495—Transforming growth factor [TGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Det er beskrevet sammensetninger og fremgangsmåter for fremstilling av et heterologt protein av interesse ved innskudd av en kopi av et gen kodende for det heterologe proteinet av interesse inn i kromosomet til en vertcelle, så som E. coli. Et kromosomalt transfer DNA (et sirkulært, ikke-selv-replikerende DNA) blir anvendt for å integrere genet kodende for det heterologe proteinet av interesse inn i vertcellekromosomet. Kromosomal transfer DNA omfatter minst en selekterbar markør og kan eventuelt innbefatte gjentatte DNA sekvenser som flankerer den selekterbare markøren, som letter den kromosomale amplifikasjonen av integrert DNA. Genet kodende for proteinet av interesse kan bli uttrykt etter integrering inn i kromosomet til vertcellen og seleksjon for kromosomal amplifikasjon kan bli utført før ekspresjon av genet.
NO975748A 1995-06-07 1997-12-05 Kromosomal ekspresjon av heterologe gener i bakterielle celler NO975748L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/482,182 US5861273A (en) 1993-12-21 1995-06-07 Chromosomal expression of heterologous genes in bacterial cells
PCT/US1996/009006 WO1996040722A1 (en) 1995-06-07 1996-06-05 Chromosomal expression of heterologous genes in bacterial cells

Publications (2)

Publication Number Publication Date
NO975748D0 NO975748D0 (no) 1997-12-05
NO975748L true NO975748L (no) 1998-01-19

Family

ID=23915047

Family Applications (1)

Application Number Title Priority Date Filing Date
NO975748A NO975748L (no) 1995-06-07 1997-12-05 Kromosomal ekspresjon av heterologe gener i bakterielle celler

Country Status (13)

Country Link
US (1) US5861273A (no)
EP (1) EP0859782A4 (no)
JP (1) JP3358818B2 (no)
KR (1) KR19990022717A (no)
CN (1) CN1190403A (no)
AU (1) AU697444B2 (no)
CA (1) CA2223494A1 (no)
CZ (1) CZ394497A3 (no)
HU (1) HUP9900868A3 (no)
NO (1) NO975748L (no)
NZ (1) NZ309645A (no)
PL (1) PL323746A1 (no)
WO (1) WO1996040722A1 (no)

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US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US5914390A (en) * 1997-05-12 1999-06-22 Celtrix Pharmaceuticals, Inc. Methods for increasing yields of recombinant proteins
US6436897B2 (en) 1998-06-01 2002-08-20 Celtrix Pharmaceuticals, Inc. Pharmaceutical formulations for IGF/IGFBP
US6417330B1 (en) * 1998-06-01 2002-07-09 Celtrix Pharmaceuticals, Inc. Insulin-like growth factor binding protein variants
DE69931928T2 (de) * 1998-07-31 2007-01-04 Gen-Probe Inc., San Diego Reversibel inhibierende sonden
US7288516B1 (en) 1999-09-20 2007-10-30 Celtrix Pharmaceuticals, Inc. Null IGF for the treatment of cancer
CA2363924A1 (en) 1999-03-02 2000-09-08 Invitrogen Corporation Compositions and methods for use in recombinational cloning of nucleic acids
US6268178B1 (en) 1999-05-25 2001-07-31 Phage Biotechnology Corp. Phage-dependent super-production of biologically active protein and peptides
DE19941186A1 (de) * 1999-08-30 2001-03-01 Peter Droege Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
CA2422625A1 (en) * 2000-09-19 2002-03-28 Bioexpertise, Llc Method for use of igf-binding protein for selective sensitization of target cells in vivo
US20050244422A1 (en) * 2004-04-16 2005-11-03 Desmond Mascarenhas Methods for delivering MBD peptide-linked agent into cells under conditions of cellular stress
US6914049B2 (en) * 2001-09-18 2005-07-05 Bioexpertise, Llc IGF-binding protein-derived peptide or small molecule
US6887851B2 (en) * 2001-09-18 2005-05-03 Bioexpertise, Llc IGF-binding protein-derived peptide
US6861406B2 (en) * 2001-09-18 2005-03-01 Bioexpertise, Llc IGF-binding protein-derived peptide
US7122715B2 (en) * 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
US6982038B2 (en) * 2002-06-14 2006-01-03 Medtronic, Inc. Centrifuge system utilizing disposable components and automated processing of blood to collect platelet rich plasma
JP5041570B2 (ja) * 2002-10-04 2012-10-03 ジェネンコー・インターナショナル・インク 生物資源の生産に対するグルコース輸送変異体
TW200502390A (en) 2002-11-28 2005-01-16 Peter Droege Sequence specific DNA recombination in eukaryotic cells
JP2007530010A (ja) 2003-06-13 2007-11-01 インスメッド，インコーポレイテッド 組換えｉｇｆ発現系
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US8034554B2 (en) 2004-07-01 2011-10-11 Gen-Probe Incorporated Methods and compositions to detect nucleic acids in a biological sample
US20060204979A1 (en) * 2004-12-01 2006-09-14 Invitrogen Corporation Reverse two-hybrid system for identification of interaction domains
AU2006311538A1 (en) * 2005-11-09 2007-05-18 Ontherix, Inc. Metal-binding therapeutic peptides
US20080039393A1 (en) * 2005-11-09 2008-02-14 Desmond Mascarenhas Metal-binding therapeutic peptides
US7611893B2 (en) 2005-11-09 2009-11-03 Ontherix, Inc. Metal-binding therapeutic peptides
US7662624B2 (en) * 2005-11-09 2010-02-16 Ontherix, Inc. Metal-binding therapeutic peptides
EP3399042A1 (en) 2007-05-17 2018-11-07 Boehringer Ingelheim RCV GmbH & Co KG Method for producing a recombinant protein on a manufacturing scale
US8536135B2 (en) * 2008-03-19 2013-09-17 Ontherix, Inc. Adaptive biochemical signatures
US20110236927A1 (en) * 2008-08-27 2011-09-29 Massachusetts Institute Of Technology Genetically stabilized tandem gene duplication
WO2010068473A1 (en) * 2008-11-25 2010-06-17 Gen-Probe Incorporated Compositions and methods for detecting small rnas, and uses thereof
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EP3728596A4 (en) 2017-12-21 2021-08-25 Glycom A/S CONSTRUCTION OF NUCLEIC ACID ALLOWING THE EXPRESSION OF A GENE IN VITRO AND IN VIVO

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US5470727A (en) * 1993-12-21 1995-11-28 Celtrix Pharmaceuticals, Inc. Chromosomal expression of non-bacterial genes in bacterial cells

Also Published As

Publication number Publication date
EP0859782A4 (en) 2002-11-06
CA2223494A1 (en) 1996-12-19
JPH11507521A (ja) 1999-07-06
KR19990022717A (ko) 1999-03-25
HUP9900868A2 (hu) 1999-06-28
AU697444B2 (en) 1998-10-08
WO1996040722A1 (en) 1996-12-19
CZ394497A3 (cs) 1998-06-17
CN1190403A (zh) 1998-08-12
NO975748D0 (no) 1997-12-05
PL323746A1 (en) 1998-04-14
HUP9900868A3 (en) 2000-04-28
JP3358818B2 (ja) 2002-12-24
NZ309645A (en) 1998-12-23
EP0859782A1 (en) 1998-08-26
AU5986496A (en) 1996-12-30
US5861273A (en) 1999-01-19

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