NO891366L - Fremgangsmaate og innretning for assaybestemmelse av oenskelig ("target") nukleotidsekvens. - Google Patents
Fremgangsmaate og innretning for assaybestemmelse av oenskelig ("target") nukleotidsekvens.Info
- Publication number
- NO891366L NO891366L NO89891366A NO891366A NO891366L NO 891366 L NO891366 L NO 891366L NO 89891366 A NO89891366 A NO 89891366A NO 891366 A NO891366 A NO 891366A NO 891366 L NO891366 L NO 891366L
- Authority
- NO
- Norway
- Prior art keywords
- target
- probe
- stated
- hybridization
- multimer
- Prior art date
Links
- 238000003556 assay Methods 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims description 35
- 239000000523 sample Substances 0.000 claims abstract description 250
- 239000002773 nucleotide Substances 0.000 claims abstract description 95
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 91
- 238000009396 hybridization Methods 0.000 claims abstract description 61
- 238000002844 melting Methods 0.000 claims abstract description 15
- 230000008018 melting Effects 0.000 claims abstract description 15
- 238000001816 cooling Methods 0.000 claims abstract description 9
- 239000002157 polynucleotide Substances 0.000 claims description 30
- 108091033319 polynucleotide Proteins 0.000 claims description 28
- 102000040430 polynucleotide Human genes 0.000 claims description 28
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 15
- 230000000295 complement effect Effects 0.000 claims description 12
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 11
- 238000000926 separation method Methods 0.000 claims description 10
- 108091034117 Oligonucleotide Proteins 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- 238000012360 testing method Methods 0.000 claims description 8
- 108020004414 DNA Proteins 0.000 claims description 6
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 6
- 239000007790 solid phase Substances 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 238000002372 labelling Methods 0.000 claims 2
- 230000003292 diminished effect Effects 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 description 16
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 7
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 6
- 239000008363 phosphate buffer Substances 0.000 description 6
- 230000037029 cross reaction Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 4
- 239000000020 Nitrocellulose Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 229920001220 nitrocellulos Polymers 0.000 description 3
- 238000010583 slow cooling Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 2
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 239000011535 reaction buffer Substances 0.000 description 2
- 208000007056 sickle cell anemia Diseases 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical group OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 241000588652 Neisseria gonorrhoeae Species 0.000 description 1
- 241000588650 Neisseria meningitidis Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 108091027568 Single-stranded nucleotide Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000002820 assay format Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 125000000548 ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8033187A | 1987-07-31 | 1987-07-31 | |
| PCT/US1988/002528 WO1989001049A1 (en) | 1987-07-31 | 1988-07-29 | Assay for polynucleotides employing oligonucleotides to eliminate undesirable cross reactions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NO891366D0 NO891366D0 (no) | 1989-03-31 |
| NO891366L true NO891366L (no) | 1989-05-23 |
Family
ID=22156716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO89891366A NO891366L (no) | 1987-07-31 | 1989-03-31 | Fremgangsmaate og innretning for assaybestemmelse av oenskelig ("target") nukleotidsekvens. |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US5434047A (ja) |
| EP (1) | EP0304184B1 (ja) |
| JP (1) | JP2783568B2 (ja) |
| KR (1) | KR960002234B1 (ja) |
| AT (1) | ATE112804T1 (ja) |
| AU (1) | AU623090B2 (ja) |
| CA (1) | CA1323552C (ja) |
| DE (1) | DE3851810T2 (ja) |
| DK (1) | DK153589A (ja) |
| ES (1) | ES2063038T3 (ja) |
| FI (1) | FI891524A0 (ja) |
| NO (1) | NO891366L (ja) |
| PT (1) | PT88144A (ja) |
| WO (1) | WO1989001049A1 (ja) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0502271A1 (en) * | 1989-04-17 | 1992-09-09 | The Standard Oil Company | 16s rRNA oligonucleotide probes for the identification of sulfate-reducing bacteria |
| JPH0380100A (ja) * | 1989-08-22 | 1991-04-04 | S R L:Kk | 核酸の塩基配列変異検出方法及び試薬 |
| EP0529070A1 (en) * | 1991-02-27 | 1993-03-03 | Amoco Corporation | Methods for improving the sensitivity of hybridization assays |
| US6200753B1 (en) * | 1993-06-03 | 2001-03-13 | Intelligene Ltd. | Detection of nucleic acid sequences |
| US5871914A (en) * | 1993-06-03 | 1999-02-16 | Intelligene Ltd. | Method for detecting a nucleic acid involving the production of a triggering RNA and transcription amplification |
| PL314522A1 (en) * | 1993-11-23 | 1996-09-16 | Ciba Corning Diagnostics Corp | Application of antisense oligomers in a contamination controlling process during nucleic acids amplification reactions |
| DE69516325T2 (de) * | 1994-02-22 | 2001-01-18 | Mitsubishi Chem Corp | Oligonukleotid und Verfahren zur Analyse der Basensequenz der Nukleinsäure |
| CA2163393C (en) * | 1994-11-30 | 2003-04-22 | Colleen Marie Nycz | Amplification and detection of mycobacteria nucleic acids |
| US6110676A (en) * | 1996-12-04 | 2000-08-29 | Boston Probes, Inc. | Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays |
| US6489455B2 (en) | 1997-05-21 | 2002-12-03 | Clontech Laboratories, Inc. | Methods of assaying differential expression |
| US5994076A (en) * | 1997-05-21 | 1999-11-30 | Clontech Laboratories, Inc. | Methods of assaying differential expression |
| US6962778B1 (en) | 1997-09-25 | 2005-11-08 | Boston Probes, Inc. | Methods, kits and compositions for suppressing the binding of detectable probes to non-target sequences in hybridization assays |
| US7981599B1 (en) | 1998-07-31 | 2011-07-19 | Boston Probes, Inc. | Non-nucleic acid probes, probe sets, methods and kits pertaining to the detection of individual human chromosomes X, Y, 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 16, 17, 18 and 20 as 13/21 as a pair |
| US6461816B1 (en) * | 1999-07-09 | 2002-10-08 | Agilent Technologies, Inc. | Methods for controlling cross-hybridization in analysis of nucleic acid sequences |
| AU2001273057A1 (en) | 2000-06-27 | 2002-01-08 | Fluidigm Corporation | A microfluidic design automation method and system |
| EP1336097A4 (en) | 2000-10-13 | 2006-02-01 | Fluidigm Corp | SAMPLE INJECTION SYSTEM USING A MICROFLUIDIC DEVICE, FOR ANALYSIS DEVICES |
| US8440093B1 (en) | 2001-10-26 | 2013-05-14 | Fuidigm Corporation | Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels |
| US7691333B2 (en) | 2001-11-30 | 2010-04-06 | Fluidigm Corporation | Microfluidic device and methods of using same |
| WO2003048295A1 (en) | 2001-11-30 | 2003-06-12 | Fluidigm Corporation | Microfluidic device and methods of using same |
| US20050145496A1 (en) | 2003-04-03 | 2005-07-07 | Federico Goodsaid | Thermal reaction device and method for using the same |
| US7476363B2 (en) | 2003-04-03 | 2009-01-13 | Fluidigm Corporation | Microfluidic devices and methods of using same |
| EP2340890B1 (en) | 2003-04-03 | 2016-10-19 | Fluidigm Corporation | Method of performimg digital PCR |
| US8828663B2 (en) | 2005-03-18 | 2014-09-09 | Fluidigm Corporation | Thermal reaction device and method for using the same |
| WO2006102264A1 (en) * | 2005-03-18 | 2006-09-28 | Fluidigm Corporation | Thermal reaction device and method for using the same |
| US7604965B2 (en) | 2003-04-03 | 2009-10-20 | Fluidigm Corporation | Thermal reaction device and method for using the same |
| US7413712B2 (en) | 2003-08-11 | 2008-08-19 | California Institute Of Technology | Microfluidic rotary flow reactor matrix |
| KR101171635B1 (ko) * | 2006-08-08 | 2012-08-09 | 아크레이 가부시키가이샤 | 변이의 검출 방법 및 그것에 이용하는 키트 |
| US7820389B2 (en) | 2007-01-10 | 2010-10-26 | Geneohm Sciences, Inc. | Inhibition of mismatch hybridization by a universal competitor DNA |
| US9260476B2 (en) | 2007-02-23 | 2016-02-16 | The Research Foundation For The State University Of New York | RNA targeting compounds and methods for making and using same |
| AU2008218939B2 (en) * | 2007-02-23 | 2014-05-08 | The Research Foundation Of State University Of New York | RNA targeting compounds and methods for making and using same |
| EP2270203A1 (en) * | 2009-06-29 | 2011-01-05 | AIT Austrian Institute of Technology GmbH | Oligonucleotide hybridization method |
| CA2811333C (en) | 2010-09-16 | 2020-05-12 | Gen-Probe Incorporated | Capture probes immobilizable via l-nucleotide tail |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI63596C (fi) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
| CA1223831A (en) * | 1982-06-23 | 1987-07-07 | Dean Engelhardt | Modified nucleotides, methods of preparing and utilizing and compositions containing the same |
| US4766062A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
| EP0172153B1 (en) * | 1984-05-15 | 1989-08-09 | Smithkline Beckman Corporation | Polynucleotide hybridization probes |
| US4683194A (en) * | 1984-05-29 | 1987-07-28 | Cetus Corporation | Method for detection of polymorphic restriction sites and nucleic acid sequences |
| US4755458A (en) * | 1984-08-30 | 1988-07-05 | Enzo Biochem, Inc. | Composition and method for the detection of the presence of a polynucleotide sequence of interest |
| FI72146C (fi) * | 1985-01-02 | 1987-04-13 | Orion Yhtymae Oy | Foerfarande foer identifiering av nukleinsyror. |
| EP0219842A1 (en) * | 1985-10-23 | 1987-04-29 | Allied Corporation | Nucleic acid assay employing pair segment inhibition or competition |
| DE3538749A1 (de) * | 1985-10-31 | 1987-05-07 | Merck Patent Gmbh | Peptide |
| WO1987003911A1 (en) * | 1985-12-17 | 1987-07-02 | Genetics Institute, Inc. | Displacement polynucleotide method and reagent complex |
| EP0232967B1 (en) * | 1986-01-10 | 1993-04-28 | Amoco Corporation | Competitive homogeneous assay |
-
1988
- 1988-07-29 PT PT88144A patent/PT88144A/pt not_active Application Discontinuation
- 1988-07-29 EP EP88307048A patent/EP0304184B1/en not_active Expired - Lifetime
- 1988-07-29 JP JP63506944A patent/JP2783568B2/ja not_active Expired - Fee Related
- 1988-07-29 WO PCT/US1988/002528 patent/WO1989001049A1/en active Application Filing
- 1988-07-29 ES ES88307048T patent/ES2063038T3/es not_active Expired - Lifetime
- 1988-07-29 CA CA000573409A patent/CA1323552C/en not_active Expired - Lifetime
- 1988-07-29 AU AU22665/88A patent/AU623090B2/en not_active Expired
- 1988-07-29 AT AT88307048T patent/ATE112804T1/de not_active IP Right Cessation
- 1988-07-29 DE DE3851810T patent/DE3851810T2/de not_active Expired - Lifetime
- 1988-07-29 KR KR1019890700566A patent/KR960002234B1/ko not_active IP Right Cessation
-
1989
- 1989-03-30 FI FI891524A patent/FI891524A0/fi not_active Application Discontinuation
- 1989-03-30 DK DK153589A patent/DK153589A/da not_active IP Right Cessation
- 1989-03-31 NO NO89891366A patent/NO891366L/no unknown
-
1993
- 1993-03-31 US US08/042,855 patent/US5434047A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU623090B2 (en) | 1992-05-07 |
| DK153589D0 (da) | 1989-03-30 |
| FI891524A (fi) | 1989-03-30 |
| PT88144A (pt) | 1989-06-30 |
| US5434047A (en) | 1995-07-18 |
| DE3851810T2 (de) | 1995-02-09 |
| AU2266588A (en) | 1989-03-01 |
| ES2063038T3 (es) | 1995-01-01 |
| WO1989001049A1 (en) | 1989-02-09 |
| ATE112804T1 (de) | 1994-10-15 |
| KR890701765A (ko) | 1989-12-21 |
| FI891524A0 (fi) | 1989-03-30 |
| DK153589A (da) | 1989-05-30 |
| EP0304184A1 (en) | 1989-02-22 |
| DE3851810D1 (de) | 1994-11-17 |
| NO891366D0 (no) | 1989-03-31 |
| JP2783568B2 (ja) | 1998-08-06 |
| KR960002234B1 (ko) | 1996-02-13 |
| JPH02500804A (ja) | 1990-03-22 |
| EP0304184B1 (en) | 1994-10-12 |
| CA1323552C (en) | 1993-10-26 |
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