NO874360L - Hybridplasmid til replikasjon i metylotrofe mikroorganismer og framgangsmaate til deres framstilling. - Google Patents
Hybridplasmid til replikasjon i metylotrofe mikroorganismer og framgangsmaate til deres framstilling.Info
- Publication number
- NO874360L NO874360L NO874360A NO874360A NO874360L NO 874360 L NO874360 L NO 874360L NO 874360 A NO874360 A NO 874360A NO 874360 A NO874360 A NO 874360A NO 874360 L NO874360 L NO 874360L
- Authority
- NO
- Norway
- Prior art keywords
- plasmid
- fragment
- pbe
- hybrid
- replication
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title claims description 105
- 230000010076 replication Effects 0.000 title claims description 17
- 238000000034 method Methods 0.000 title claims description 9
- 244000005700 microbiome Species 0.000 title description 2
- 238000002360 preparation method Methods 0.000 title description 2
- 239000012634 fragment Substances 0.000 claims description 51
- 241000589341 Methylomonas clara Species 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 11
- 230000029087 digestion Effects 0.000 claims description 9
- 238000010276 construction Methods 0.000 claims description 7
- 102000003960 Ligases Human genes 0.000 claims description 5
- 108090000364 Ligases Proteins 0.000 claims description 5
- 239000013613 expression plasmid Substances 0.000 claims description 4
- 102000003929 Transaminases Human genes 0.000 claims description 3
- 108090000340 Transaminases Proteins 0.000 claims description 3
- 125000003118 aryl group Chemical group 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 21
- 102000004190 Enzymes Human genes 0.000 description 15
- 108090000790 Enzymes Proteins 0.000 description 15
- 108091008146 restriction endonucleases Proteins 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 239000000872 buffer Substances 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 239000004098 Tetracycline Substances 0.000 description 7
- 229960002180 tetracycline Drugs 0.000 description 7
- 229930101283 tetracycline Natural products 0.000 description 7
- 235000019364 tetracycline Nutrition 0.000 description 7
- 150000003522 tetracyclines Chemical class 0.000 description 7
- 239000013598 vector Substances 0.000 description 7
- 108091028043 Nucleic acid sequence Proteins 0.000 description 6
- 229960000723 ampicillin Drugs 0.000 description 6
- 230000021615 conjugation Effects 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 5
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 5
- 102000004594 DNA Polymerase I Human genes 0.000 description 4
- 108010017826 DNA Polymerase I Proteins 0.000 description 4
- 238000000246 agarose gel electrophoresis Methods 0.000 description 4
- 238000000502 dialysis Methods 0.000 description 4
- 102000012410 DNA Ligases Human genes 0.000 description 3
- 108010061982 DNA Ligases Proteins 0.000 description 3
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 3
- 239000007984 Tris EDTA buffer Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 101150028338 tyrB gene Proteins 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 241001131785 Escherichia coli HB101 Species 0.000 description 2
- 241001646716 Escherichia coli K-12 Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19863635583 DE3635583A1 (de) | 1986-10-20 | 1986-10-20 | Hybridplasmid zur replikation in methylotrophen mikroorganismen und verfahren zu dessen herstellung |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NO874360D0 NO874360D0 (no) | 1987-10-19 |
| NO874360L true NO874360L (no) | 1988-04-21 |
Family
ID=6312037
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO874360A NO874360L (no) | 1986-10-20 | 1987-10-19 | Hybridplasmid til replikasjon i metylotrofe mikroorganismer og framgangsmaate til deres framstilling. |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP0264805A2 (xx) |
| JP (1) | JPS63185388A (xx) |
| KR (1) | KR880005266A (xx) |
| CN (1) | CN87106979A (xx) |
| AU (1) | AU7989987A (xx) |
| DE (1) | DE3635583A1 (xx) |
| DK (1) | DK545287A (xx) |
| FI (1) | FI874565A (xx) |
| IE (1) | IE872805L (xx) |
| IL (1) | IL84192A0 (xx) |
| NO (1) | NO874360L (xx) |
| PT (1) | PT85940A (xx) |
| ZA (1) | ZA877829B (xx) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3720834A1 (de) * | 1987-06-24 | 1989-01-05 | Hoechst Ag | Promotoren zur expression von fremd-dna in methylotrophen bakterien, deren gewinnung und deren verwendung |
| KR102123512B1 (ko) * | 2019-03-12 | 2020-06-16 | 한국에너지기술연구원 | 메타노트로프(Methanotroph)에서 유래한 신규 메타노트로프-대장균 셔틀 벡터 |
-
1986
- 1986-10-20 DE DE19863635583 patent/DE3635583A1/de not_active Withdrawn
-
1987
- 1987-10-14 EP EP87114995A patent/EP0264805A2/de not_active Withdrawn
- 1987-10-16 FI FI874565A patent/FI874565A/fi not_active Application Discontinuation
- 1987-10-18 IL IL84192A patent/IL84192A0/xx unknown
- 1987-10-19 ZA ZA877829A patent/ZA877829B/xx unknown
- 1987-10-19 DK DK545287A patent/DK545287A/da not_active Application Discontinuation
- 1987-10-19 KR KR870011565A patent/KR880005266A/ko not_active Application Discontinuation
- 1987-10-19 AU AU79899/87A patent/AU7989987A/en not_active Abandoned
- 1987-10-19 PT PT85940A patent/PT85940A/pt unknown
- 1987-10-19 NO NO874360A patent/NO874360L/no unknown
- 1987-10-19 CN CN198787106979A patent/CN87106979A/zh active Pending
- 1987-10-19 IE IE872805A patent/IE872805L/xx unknown
- 1987-10-20 JP JP62265193A patent/JPS63185388A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| IL84192A0 (en) | 1988-03-31 |
| JPS63185388A (ja) | 1988-07-30 |
| KR880005266A (ko) | 1988-06-28 |
| NO874360D0 (no) | 1987-10-19 |
| PT85940A (de) | 1987-11-01 |
| DK545287A (da) | 1988-04-21 |
| ZA877829B (en) | 1988-04-22 |
| AU7989987A (en) | 1988-04-21 |
| EP0264805A2 (de) | 1988-04-27 |
| FI874565A (fi) | 1988-04-21 |
| DE3635583A1 (de) | 1988-04-21 |
| CN87106979A (zh) | 1988-07-06 |
| FI874565A0 (fi) | 1987-10-16 |
| IE872805L (en) | 1988-04-20 |
| DK545287D0 (da) | 1987-10-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0331961B1 (en) | Method for protein synthesis using CKS fusion proteins | |
| Mizuuchi et al. | Efficient Mu transposition requires interaction of transposase with a DNA sequence at the Mu operator: implications for regulation | |
| JPH0543348B2 (xx) | ||
| WO1992014819A1 (en) | A positive selection vector for the bacteriophage p1 cloning system | |
| Timmis et al. | Plasmid incompatibility: cloning analysis of an inc FII determinant of R6-5 | |
| HU205386B (en) | Process for expressing cloned lysostaphin gene and for producing dna fragment containing the gene, expression vector and transformed host cell | |
| Hinchliffe et al. | Expression of the cloned endo-1, 3-1, 4-β-glucanase gene of Bacillus subtilis in Saccharomyces cerevisiae | |
| EP0315637B1 (en) | Polypeptides useful in diagnosis of mycoplasma infections in swine and recombinant-dna methods for manufacturing same | |
| JPH1084978A (ja) | 改良されたリボフラビン生産 | |
| EP0654084A1 (en) | Molecular cloning of the genes reponsible for collagenase production from clostridium histolyticum | |
| US4828988A (en) | Hybrid polypeptides comprising somatocrinine and alpha1 -antitrypsin, method for their production from bacterial clones and use thereof for the production of somatocrinine | |
| WO1984000774A1 (en) | Staphylococcal protein a coding gene (dna) fragment comprising a signal dna sequence, a process for its preparation and a microorganism transformed therewith | |
| EP0133756A2 (en) | Vector for expression of polypeptides | |
| NO175153B (no) | Polypeptider | |
| US5312737A (en) | CKS method of HCV protein synthesis | |
| NO874360L (no) | Hybridplasmid til replikasjon i metylotrofe mikroorganismer og framgangsmaate til deres framstilling. | |
| JPS63157987A (ja) | 遺伝子発現系 | |
| US5312901A (en) | Cloned streptococcal genes encoding protein G and their use to construct recombinant microorganisms to produce protein G | |
| EP0091723A2 (en) | Vector system | |
| DK172458B1 (da) | Signalpeptid, DNA-sekvens, som koder for dette peptid, genstruktur indeholdende DNA-sekvensen, plasmid indeholdende DNA-sek | |
| US4992371A (en) | Hybrid plasmids having a streptomycetes replicon and an escherichia coli replicon | |
| JP2540039B2 (ja) | プラスミドベクタ− | |
| EP0108301A2 (en) | Method of heterologous cloning of gene in bacillus microorganism | |
| US4977089A (en) | Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms | |
| JPS62215393A (ja) | バチルス中でのヒト血清アルブミンの製造 |