NO841810L - Fremgangsmaate ved fremstilling av dextran-sakkarase - Google Patents
Fremgangsmaate ved fremstilling av dextran-sakkaraseInfo
- Publication number
- NO841810L NO841810L NO841810A NO841810A NO841810L NO 841810 L NO841810 L NO 841810L NO 841810 A NO841810 A NO 841810A NO 841810 A NO841810 A NO 841810A NO 841810 L NO841810 L NO 841810L
- Authority
- NO
- Norway
- Prior art keywords
- dextran
- medium
- phase
- sucrose
- enzyme
- Prior art date
Links
- 229920002307 Dextran Polymers 0.000 title claims description 42
- 238000000034 method Methods 0.000 title claims description 30
- 238000002360 preparation method Methods 0.000 title claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 28
- 102000004190 Enzymes Human genes 0.000 claims description 28
- 239000002609 medium Substances 0.000 claims description 22
- 238000000746 purification Methods 0.000 claims description 21
- 239000005720 sucrose Substances 0.000 claims description 21
- 229930006000 Sucrose Natural products 0.000 claims description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 13
- 239000000243 solution Substances 0.000 claims description 11
- 229920000570 polyether Polymers 0.000 claims description 10
- 239000004721 Polyphenylene oxide Substances 0.000 claims description 9
- 241000192130 Leuconostoc mesenteroides Species 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 229920000642 polymer Polymers 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 238000000622 liquid--liquid extraction Methods 0.000 claims description 4
- 238000000638 solvent extraction Methods 0.000 claims description 4
- 230000007774 longterm Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims 1
- 229920000151 polyglycol Polymers 0.000 claims 1
- 239000010695 polyglycol Substances 0.000 claims 1
- 239000012071 phase Substances 0.000 description 49
- 230000000694 effects Effects 0.000 description 23
- 239000002202 Polyethylene glycol Substances 0.000 description 17
- 229920001223 polyethylene glycol Polymers 0.000 description 17
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 15
- 239000008103 glucose Substances 0.000 description 15
- 238000009826 distribution Methods 0.000 description 14
- 235000011073 invertase Nutrition 0.000 description 14
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 13
- 229930091371 Fructose Natural products 0.000 description 11
- 239000005715 Fructose Substances 0.000 description 11
- 239000001573 invertase Substances 0.000 description 11
- 239000006228 supernatant Substances 0.000 description 11
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 10
- 230000002255 enzymatic effect Effects 0.000 description 9
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000001523 saccharolytic effect Effects 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 4
- 235000000346 sugar Nutrition 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000000356 contaminant Substances 0.000 description 3
- 108010042194 dextransucrase Proteins 0.000 description 3
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 241000192132 Leuconostoc Species 0.000 description 2
- 229920002535 Polyethylene Glycol 1500 Polymers 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 239000007974 sodium acetate buffer Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- VUYXVWGKCKTUMF-UHFFFAOYSA-N tetratriacontaethylene glycol monomethyl ether Chemical compound COCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO VUYXVWGKCKTUMF-UHFFFAOYSA-N 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- WDMUXYQIMRDWRC-UHFFFAOYSA-N 2-hydroxy-3,4-dinitrobenzoic acid Chemical compound OC(=O)C1=CC=C([N+]([O-])=O)C([N+]([O-])=O)=C1O WDMUXYQIMRDWRC-UHFFFAOYSA-N 0.000 description 1
- 102100031126 6-phosphogluconolactonase Human genes 0.000 description 1
- 108010029731 6-phosphogluconolactonase Proteins 0.000 description 1
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 1
- 102000005548 Hexokinase Human genes 0.000 description 1
- 108700040460 Hexokinases Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 108010036940 Levansucrase Proteins 0.000 description 1
- XJLXINKUBYWONI-NNYOXOHSSA-O NADP(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-O 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 101710184309 Probable sucrose-6-phosphate hydrolase Proteins 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 102400000472 Sucrase Human genes 0.000 description 1
- 101710112652 Sucrose-6-phosphate hydrolase Proteins 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 241000385732 bacterium L Species 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000009229 glucose formation Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000012070 reactive reagent Substances 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/816—Enzyme separation or purification by solubility
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8307650A FR2545501B1 (fr) | 1983-05-06 | 1983-05-06 | Procede de purification de la dextrane-saccharase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO841810L true NO841810L (no) | 1984-11-07 |
Family
ID=9288678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO841810A NO841810L (no) | 1983-05-06 | 1984-05-04 | Fremgangsmaate ved fremstilling av dextran-sakkarase |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US4591563A (da) |
| EP (1) | EP0125981B1 (da) |
| JP (1) | JPS6091982A (da) |
| BR (1) | BR8402101A (da) |
| CA (1) | CA1213232A (da) |
| DE (1) | DE3462609D1 (da) |
| DK (1) | DK222984A (da) |
| FR (1) | FR2545501B1 (da) |
| IL (1) | IL71754A (da) |
| NO (1) | NO841810L (da) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4728613A (en) * | 1985-09-04 | 1988-03-01 | Miles Laboratories, Inc. | Method for the recovery of extracellular enzymes from whole fermentation beer |
| US5139943A (en) * | 1989-06-13 | 1992-08-18 | Genencor International, Inc. | Processes for the recovery of microbially produced chymosin |
| US5151358A (en) * | 1989-06-13 | 1992-09-29 | Genencor International, Inc. | Processes for the recovery of naturally produced chymosin |
| US5290474A (en) * | 1990-10-05 | 1994-03-01 | Genencor International, Inc. | Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp |
| US5328841A (en) * | 1990-10-05 | 1994-07-12 | Genencor International, Inc. | Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol |
| CA2093422C (en) * | 1990-10-05 | 2001-04-03 | DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS | |
| GB2256869B (en) * | 1991-06-14 | 1995-07-19 | Mitsubishi Chem Ind | Process for preparing sucrose fatty acid esters |
| JPH0668501U (ja) * | 1993-03-11 | 1994-09-27 | 聚上企業股▲ひん▼有限公司 | 滑り止めおよび爪先ガード機能付きスリッパ |
| WO2003096821A2 (en) * | 2002-05-21 | 2003-11-27 | Unilever Plc | Frozen aerated product in an aerosol container |
| ATE410069T1 (de) * | 2003-11-25 | 2008-10-15 | Unilever Nv | Verfahren zur abgabe eines lebensmittels |
| EP1761552B1 (en) * | 2004-06-29 | 2008-09-17 | Ares Trading S.A. | Process for the purification of il-18 binding protein |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1589581A (en) * | 1976-04-14 | 1981-05-13 | Biotechnolog Forschung Gmbh | Process for the separation of enzymes |
| GB2079290B (en) * | 1980-07-05 | 1984-05-16 | Fisons Ltd | Preparation and purification process |
| US4508825A (en) * | 1983-03-30 | 1985-04-02 | Miles Laboratories, Inc. | Method for the separation of extracellular amylase and protease produced during the fermentation of enzyme producing microorganisms |
-
1983
- 1983-05-06 FR FR8307650A patent/FR2545501B1/fr not_active Expired
-
1984
- 1984-05-01 CA CA000453262A patent/CA1213232A/fr not_active Expired
- 1984-05-03 DE DE8484400909T patent/DE3462609D1/de not_active Expired
- 1984-05-03 US US06/606,642 patent/US4591563A/en not_active Expired - Fee Related
- 1984-05-03 EP EP84400909A patent/EP0125981B1/fr not_active Expired
- 1984-05-04 JP JP59088553A patent/JPS6091982A/ja active Pending
- 1984-05-04 NO NO841810A patent/NO841810L/no unknown
- 1984-05-04 DK DK222984A patent/DK222984A/da not_active Application Discontinuation
- 1984-05-04 IL IL71754A patent/IL71754A/xx unknown
- 1984-05-04 BR BR8402101A patent/BR8402101A/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US4591563A (en) | 1986-05-27 |
| DE3462609D1 (en) | 1987-04-16 |
| EP0125981A1 (fr) | 1984-11-21 |
| BR8402101A (pt) | 1984-12-11 |
| DK222984D0 (da) | 1984-05-04 |
| FR2545501B1 (fr) | 1985-08-02 |
| EP0125981B1 (fr) | 1987-03-11 |
| CA1213232A (fr) | 1986-10-28 |
| DK222984A (da) | 1984-11-07 |
| IL71754A0 (en) | 1984-09-30 |
| IL71754A (en) | 1987-10-20 |
| JPS6091982A (ja) | 1985-05-23 |
| FR2545501A1 (fr) | 1984-11-09 |
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