NO314091B1 - Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet - Google Patents
Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet Download PDFInfo
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- NO314091B1 NO314091B1 NO20005428A NO20005428A NO314091B1 NO 314091 B1 NO314091 B1 NO 314091B1 NO 20005428 A NO20005428 A NO 20005428A NO 20005428 A NO20005428 A NO 20005428A NO 314091 B1 NO314091 B1 NO 314091B1
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- dna
- uracil
- enzyme
- ung
- activity
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- 238000001914 filtration Methods 0.000 description 1
- IVSXFFJGASXYCL-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=NC=N[C]21 IVSXFFJGASXYCL-UHFFFAOYSA-N 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
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- GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 1
- 108010052968 leupeptin Proteins 0.000 description 1
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- YFCUZWYIPBUQBD-ZOWNYOTGSA-N n-[(3s)-7-amino-1-chloro-2-oxoheptan-3-yl]-4-methylbenzenesulfonamide;hydron;chloride Chemical compound Cl.CC1=CC=C(S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl)C=C1 YFCUZWYIPBUQBD-ZOWNYOTGSA-N 0.000 description 1
- 239000006199 nebulizer Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 239000006174 pH buffer Substances 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229950000964 pepstatin Drugs 0.000 description 1
- 108010091212 pepstatin Proteins 0.000 description 1
- FAXGPCHRFPCXOO-LXTPJMTPSA-N pepstatin A Chemical compound OC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C FAXGPCHRFPCXOO-LXTPJMTPSA-N 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
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- 239000011546 protein dye Substances 0.000 description 1
- 229940121649 protein inhibitor Drugs 0.000 description 1
- 239000012268 protein inhibitor Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000010850 salt effect Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- PGAVKCOVUIYSFO-UHFFFAOYSA-N uridine-triphosphate Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)OC1N1C(=O)NC(=O)C=C1 PGAVKCOVUIYSFO-UHFFFAOYSA-N 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
- C12Y302/02027—Uracil-DNA glycosylase (3.2.2.27)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NO20005428A NO314091B1 (no) | 2000-01-12 | 2000-10-27 | Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet |
| JP2001551197A JP4808352B2 (ja) | 2000-01-12 | 2001-01-10 | タラウラシル−ｄｎａグリコシラーゼ、これをコードする遺伝子、該遺伝子またはその機能性部分を含有する組換えｄｎａ、該タンパク質の調製法と、ｐｃｒの追跡もしくは調節における該タンパク質またはその機能性部分の使用 |
| DE60110893T DE60110893T2 (de) | 2000-01-12 | 2001-01-10 | Kabeljau-spezifische urazil-dna glykosilase, dafür kodierendes gen, selbiges gen beinhaltende, rekombinante dna oder operative teile davon, ein verfahren zur herstellung des proteins und die anwendung des proteins oder der operativen teile zur überwachung und kontrolle der pcr |
| PCT/NO2001/000008 WO2001051623A1 (fr) | 2000-01-12 | 2001-01-10 | Glycosylase d'adn d'uracil de morue, gene codant ce dernier, adn contenant ce gene ou des parties operationnelles de ce dernier, procede de preparation de ladite proteine et utilisation de celle-ci ou des parties operationnelles pour surveiller ou controler la reaction pcr |
| AT01900799T ATE295878T1 (de) | 2000-01-12 | 2001-01-10 | Kabeljau-spezifische urazil-dna glykosilase, dafür kodierendes gen, selbiges gen beinhaltende, rekombinante dna oder operative teile davon, ein verfahren zur herstellung des proteins und die anwendung des protein oder der operativen teile zur überwachung und kontrolle der pcr |
| US09/758,017 US7037703B2 (en) | 2000-01-12 | 2001-01-10 | Cod uracil-dna glycosylase, gene coding therefore, recombinant dna containing said gene or operative parts thereof, a method for preparing said protein and the use of said protein or said operative parts thereof in monitoring or controlling pcr |
| EP01900799A EP1246908B1 (fr) | 2000-01-12 | 2001-01-10 | Glycosylase d'adn d'uracil de morue, gene codant ce dernier, adn contenant ce gene ou des parties operationnelles de ce dernier, procede de preparation de ladite proteine et utilisation de celle-ci ou des parties operationnelles pour surveiller ou controler la reaction pcr |
| AU25596/01A AU784783B2 (en) | 2000-01-12 | 2001-01-10 | Cod uracil-DNA glycosylase, gene coding therefore, recombinant DNA containing said gene or operative parts thereof, a method for preparing said protein and the use of said protein or said operative parts thereof in monitoring or controlling PCR |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NO20000163A NO20000163L (no) | 2000-01-12 | 2000-01-12 | Varmelabil uracil-DNA-glykosylase samt anvendelse derav |
| NO20005428A NO314091B1 (no) | 2000-01-12 | 2000-10-27 | Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO20005428D0 NO20005428D0 (no) | 2000-10-27 |
| NO20005428L NO20005428L (no) | 2001-07-13 |
| NO314091B1 true NO314091B1 (no) | 2003-01-27 |
Family
ID=26649192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO20005428A NO314091B1 (no) | 2000-01-12 | 2000-10-27 | Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7037703B2 (fr) |
| EP (1) | EP1246908B1 (fr) |
| JP (1) | JP4808352B2 (fr) |
| AT (1) | ATE295878T1 (fr) |
| AU (1) | AU784783B2 (fr) |
| DE (1) | DE60110893T2 (fr) |
| NO (1) | NO314091B1 (fr) |
| WO (1) | WO2001051623A1 (fr) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1415005B1 (fr) | 2000-12-07 | 2012-11-21 | Novartis Vaccines and Diagnostics, Inc. | Retrovirus endogenes regules positivement dans le cancer de la prostate |
| US7399590B2 (en) * | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| EP2290096B1 (fr) | 2002-02-21 | 2014-11-19 | Alere San Diego, Inc. | Amplification par recombinase et polymérase utilisant un agent de recombinaison sensible à la température |
| US8518694B2 (en) | 2002-06-13 | 2013-08-27 | Novartis Vaccines And Diagnostics, Inc. | Nucleic acid vector comprising a promoter and a sequence encoding a polypeptide from the endogenous retrovirus PCAV |
| CA2616241C (fr) * | 2005-07-25 | 2012-02-07 | Asm Scientific, Inc. | Procedes d'amplification multiple par recombinase-polymerase |
| EP3088533B1 (fr) | 2006-05-04 | 2018-01-17 | Alere San Diego, Inc. | Amplification par recombinase polymérase |
| KR100963014B1 (ko) | 2007-10-18 | 2010-06-09 | 성균관대학교산학협력단 | 바실러스 스피시스 нｊ171 균주 유래의 신규한 저온성우라실-ｄｎａ 글리코실라제의 제조방법 및 중합효소연쇄반응에서의 이용 |
| WO2010135310A1 (fr) | 2009-05-20 | 2010-11-25 | Biosite Incorporated | Glycosylase/lyase d'adn et substrats d'endonucléase ap |
| EP3360974A1 (fr) | 2009-06-05 | 2018-08-15 | Alere San Diego, Inc. | Réactifs d'amplification par recombinase polymérase |
| US8563298B2 (en) | 2010-10-22 | 2013-10-22 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
| CA2815085C (fr) | 2010-10-22 | 2022-06-21 | T2 Biosystems, Inc. | Systemes de rmn et procedes de detection rapide d'analytes |
| EP3202918B1 (fr) | 2011-04-07 | 2021-10-20 | Abbott Diagnostics Scarborough, Inc. | Surveillance de mélanges d'amplification de recombinase-polymérase |
| GB201611469D0 (en) * | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
| CN107828758A (zh) * | 2017-11-13 | 2018-03-23 | 江苏众红生物工程创药研究院有限公司 | 重组尿嘧啶‑dna糖苷酶及其编码基因、制备方法和应用 |
| GB2569965A (en) | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
| CN112342231A (zh) * | 2020-10-30 | 2021-02-09 | 厦门大学 | 一种不耐热ung融合蛋白的重组载体及表达纯化方法 |
| WO2022232087A1 (fr) * | 2021-04-30 | 2022-11-03 | Nutcracker Therapeutics, Inc. | Procédés pour effectuer une transcription in vitro à l'aide de dutp |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19545320A1 (de) * | 1995-12-05 | 1997-06-12 | Boehringer Mannheim Gmbh | Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA |
| GB9600384D0 (en) | 1996-01-09 | 1996-03-13 | Nyfotek As | Dna glycosylases |
-
2000
- 2000-10-27 NO NO20005428A patent/NO314091B1/no not_active IP Right Cessation
-
2001
- 2001-01-10 DE DE60110893T patent/DE60110893T2/de not_active Expired - Lifetime
- 2001-01-10 AT AT01900799T patent/ATE295878T1/de not_active IP Right Cessation
- 2001-01-10 EP EP01900799A patent/EP1246908B1/fr not_active Expired - Lifetime
- 2001-01-10 US US09/758,017 patent/US7037703B2/en not_active Expired - Lifetime
- 2001-01-10 AU AU25596/01A patent/AU784783B2/en not_active Expired
- 2001-01-10 JP JP2001551197A patent/JP4808352B2/ja not_active Expired - Lifetime
- 2001-01-10 WO PCT/NO2001/000008 patent/WO2001051623A1/fr active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| AU784783B2 (en) | 2006-06-15 |
| AU2559601A (en) | 2001-07-24 |
| DE60110893T2 (de) | 2006-04-27 |
| US7037703B2 (en) | 2006-05-02 |
| EP1246908B1 (fr) | 2005-05-18 |
| NO20005428L (no) | 2001-07-13 |
| DE60110893D1 (de) | 2005-06-23 |
| ATE295878T1 (de) | 2005-06-15 |
| NO20005428D0 (no) | 2000-10-27 |
| US20020155573A1 (en) | 2002-10-24 |
| EP1246908A1 (fr) | 2002-10-09 |
| WO2001051623A1 (fr) | 2001-07-19 |
| JP4808352B2 (ja) | 2011-11-02 |
| JP2003519490A (ja) | 2003-06-24 |
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