NO307341B1 - Nye T-celle lymfom cDNA kloner og fremstilling av T- lymfomcelleprotein - Google Patents
Nye T-celle lymfom cDNA kloner og fremstilling av T- lymfomcelleprotein Download PDFInfo
- Publication number
- NO307341B1 NO307341B1 NO923918A NO923918A NO307341B1 NO 307341 B1 NO307341 B1 NO 307341B1 NO 923918 A NO923918 A NO 923918A NO 923918 A NO923918 A NO 923918A NO 307341 B1 NO307341 B1 NO 307341B1
- Authority
- NO
- Norway
- Prior art keywords
- cells
- cell
- cdna
- protein
- gene
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 title claims abstract description 246
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 232
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 131
- 206010025323 Lymphomas Diseases 0.000 title claims abstract description 35
- 239000002299 complementary DNA Substances 0.000 title claims description 152
- 238000004519 manufacturing process Methods 0.000 title claims description 10
- 206010042971 T-cell lymphoma Diseases 0.000 title 1
- 208000027585 T-cell non-Hodgkin lymphoma Diseases 0.000 title 1
- 210000001744 T-lymphocyte Anatomy 0.000 claims abstract description 72
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 38
- 238000000034 method Methods 0.000 claims abstract description 22
- 108020004511 Recombinant DNA Proteins 0.000 claims abstract description 11
- 230000008569 process Effects 0.000 claims abstract description 4
- 230000014509 gene expression Effects 0.000 claims description 104
- 239000013598 vector Substances 0.000 claims description 16
- 230000027455 binding Effects 0.000 claims description 9
- 239000013612 plasmid Substances 0.000 claims description 9
- 230000010076 replication Effects 0.000 claims description 6
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 3
- 102000053602 DNA Human genes 0.000 claims description 3
- 241000588724 Escherichia coli Species 0.000 claims description 2
- 230000001131 transforming effect Effects 0.000 claims description 2
- 210000001519 tissue Anatomy 0.000 abstract description 35
- 238000011161 development Methods 0.000 abstract description 17
- 230000018109 developmental process Effects 0.000 abstract description 17
- 210000004837 gut-associated lymphoid tissue Anatomy 0.000 abstract description 17
- 210000001541 thymus gland Anatomy 0.000 abstract description 16
- 239000012528 membrane Substances 0.000 abstract description 13
- 210000004185 liver Anatomy 0.000 abstract description 12
- 230000002611 ovarian Effects 0.000 abstract description 7
- 210000004989 spleen cell Anatomy 0.000 abstract description 6
- 108091005703 transmembrane proteins Proteins 0.000 abstract description 6
- 102000035160 transmembrane proteins Human genes 0.000 abstract description 6
- 230000013020 embryo development Effects 0.000 abstract description 2
- 101150060800 lov gene Proteins 0.000 description 81
- 108020004414 DNA Proteins 0.000 description 66
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 44
- 108020004999 messenger RNA Proteins 0.000 description 41
- 150000001413 amino acids Chemical group 0.000 description 36
- 210000000349 chromosome Anatomy 0.000 description 35
- 239000000523 sample Substances 0.000 description 33
- 238000004458 analytical method Methods 0.000 description 31
- 101150096021 tea gene Proteins 0.000 description 30
- 210000004754 hybrid cell Anatomy 0.000 description 29
- 241000699666 Mus <mouse, genus> Species 0.000 description 27
- 238000009396 hybridization Methods 0.000 description 26
- 241001529936 Murinae Species 0.000 description 23
- 238000000636 Northern blotting Methods 0.000 description 21
- 230000002992 thymic effect Effects 0.000 description 20
- 206010028980 Neoplasm Diseases 0.000 description 19
- 239000000427 antigen Substances 0.000 description 17
- 108091007433 antigens Proteins 0.000 description 17
- 102000036639 antigens Human genes 0.000 description 17
- 239000012634 fragment Substances 0.000 description 17
- 210000001672 ovary Anatomy 0.000 description 17
- 241000282414 Homo sapiens Species 0.000 description 16
- 241000699670 Mus sp. Species 0.000 description 14
- 230000006698 induction Effects 0.000 description 14
- 241001465754 Metazoa Species 0.000 description 13
- 102000005962 receptors Human genes 0.000 description 13
- 108020003175 receptors Proteins 0.000 description 13
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 12
- 238000002105 Southern blotting Methods 0.000 description 12
- 230000006870 function Effects 0.000 description 12
- 241000894007 species Species 0.000 description 12
- 238000003501 co-culture Methods 0.000 description 11
- 102100036011 T-cell surface glycoprotein CD4 Human genes 0.000 description 10
- 201000011510 cancer Diseases 0.000 description 10
- 108090000765 processed proteins & peptides Proteins 0.000 description 10
- 210000004988 splenocyte Anatomy 0.000 description 10
- 108010038807 Oligopeptides Proteins 0.000 description 9
- 102000015636 Oligopeptides Human genes 0.000 description 9
- 230000000704 physical effect Effects 0.000 description 9
- 230000001177 retroviral effect Effects 0.000 description 9
- 102000007469 Actins Human genes 0.000 description 8
- 108010085238 Actins Proteins 0.000 description 8
- 108091026890 Coding region Proteins 0.000 description 8
- 206010033128 Ovarian cancer Diseases 0.000 description 8
- 230000004913 activation Effects 0.000 description 8
- 238000001994 activation Methods 0.000 description 8
- 230000000295 complement effect Effects 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- 241000699800 Cricetinae Species 0.000 description 7
- 238000002965 ELISA Methods 0.000 description 7
- 108010052285 Membrane Proteins Proteins 0.000 description 7
- 241000283973 Oryctolagus cuniculus Species 0.000 description 7
- 230000006044 T cell activation Effects 0.000 description 7
- 239000011543 agarose gel Substances 0.000 description 7
- 210000004698 lymphocyte Anatomy 0.000 description 7
- 230000035800 maturation Effects 0.000 description 7
- 238000011160 research Methods 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- 238000010186 staining Methods 0.000 description 7
- 108020004635 Complementary DNA Proteins 0.000 description 6
- 101100356020 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) recA gene Proteins 0.000 description 6
- 102000018697 Membrane Proteins Human genes 0.000 description 6
- 101100042680 Mus musculus Slc7a1 gene Proteins 0.000 description 6
- 108700026244 Open Reading Frames Proteins 0.000 description 6
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 6
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 6
- 102100034922 T-cell surface glycoprotein CD8 alpha chain Human genes 0.000 description 6
- 238000012512 characterization method Methods 0.000 description 6
- 230000001086 cytosolic effect Effects 0.000 description 6
- 238000002955 isolation Methods 0.000 description 6
- 239000002773 nucleotide Substances 0.000 description 6
- 125000003729 nucleotide group Chemical group 0.000 description 6
- 108091008146 restriction endonucleases Proteins 0.000 description 6
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 6
- 101150062364 19.5 gene Proteins 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 5
- 238000000211 autoradiogram Methods 0.000 description 5
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 5
- 230000033228 biological regulation Effects 0.000 description 5
- 210000000170 cell membrane Anatomy 0.000 description 5
- 108091092328 cellular RNA Proteins 0.000 description 5
- 230000001413 cellular effect Effects 0.000 description 5
- 230000000875 corresponding effect Effects 0.000 description 5
- 230000004069 differentiation Effects 0.000 description 5
- 210000003386 epithelial cell of thymus gland Anatomy 0.000 description 5
- 230000004927 fusion Effects 0.000 description 5
- 210000003563 lymphoid tissue Anatomy 0.000 description 5
- 239000006166 lysate Substances 0.000 description 5
- 238000013507 mapping Methods 0.000 description 5
- 239000003226 mitogen Substances 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 5
- 210000004881 tumor cell Anatomy 0.000 description 5
- 101150096316 5 gene Proteins 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 108010062580 Concanavalin A Proteins 0.000 description 4
- 241000699660 Mus musculus Species 0.000 description 4
- 239000000020 Nitrocellulose Substances 0.000 description 4
- 238000005520 cutting process Methods 0.000 description 4
- 210000000805 cytoplasm Anatomy 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 4
- 210000002216 heart Anatomy 0.000 description 4
- 210000004201 immune sera Anatomy 0.000 description 4
- 229940042743 immune sera Drugs 0.000 description 4
- 238000001114 immunoprecipitation Methods 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 238000010369 molecular cloning Methods 0.000 description 4
- 229920001220 nitrocellulos Polymers 0.000 description 4
- 230000008488 polyadenylation Effects 0.000 description 4
- 239000002243 precursor Substances 0.000 description 4
- 230000008707 rearrangement Effects 0.000 description 4
- 230000003393 splenic effect Effects 0.000 description 4
- 230000000381 tumorigenic effect Effects 0.000 description 4
- 241001430294 unidentified retrovirus Species 0.000 description 4
- 108020005544 Antisense RNA Proteins 0.000 description 3
- 241000699802 Cricetulus griseus Species 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 241000701959 Escherichia virus Lambda Species 0.000 description 3
- 241000206602 Eukaryota Species 0.000 description 3
- 241000287828 Gallus gallus Species 0.000 description 3
- 102100029217 High affinity cationic amino acid transporter 1 Human genes 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 101000633751 Homo sapiens High affinity cationic amino acid transporter 1 Proteins 0.000 description 3
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
- 108020003217 Nuclear RNA Proteins 0.000 description 3
- 102000043141 Nuclear RNA Human genes 0.000 description 3
- 229930182555 Penicillin Natural products 0.000 description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 3
- 108010076504 Protein Sorting Signals Proteins 0.000 description 3
- 108091034057 RNA (poly(A)) Proteins 0.000 description 3
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- 108091008874 T cell receptors Proteins 0.000 description 3
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 3
- 235000013330 chicken meat Nutrition 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 239000003184 complementary RNA Substances 0.000 description 3
- 108091092330 cytoplasmic RNA Proteins 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 210000000981 epithelium Anatomy 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 239000012894 fetal calf serum Substances 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 239000003446 ligand Substances 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 229930182817 methionine Natural products 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 229940049954 penicillin Drugs 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 238000011451 sequencing strategy Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 239000002356 single layer Substances 0.000 description 3
- 239000000344 soap Substances 0.000 description 3
- 210000000952 spleen Anatomy 0.000 description 3
- 229960005322 streptomycin Drugs 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 231100000588 tumorigenic Toxicity 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 238000011725 BALB/c mouse Methods 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 108010078791 Carrier Proteins Proteins 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 108010038453 Interleukin-2 Receptors Proteins 0.000 description 2
- 102000010789 Interleukin-2 Receptors Human genes 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 241000283203 Otariidae Species 0.000 description 2
- 101150005926 Pc gene Proteins 0.000 description 2
- 241000276498 Pollachius virens Species 0.000 description 2
- 241000283984 Rodentia Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 210000000173 T-lymphoid precursor cell Anatomy 0.000 description 2
- 101150052863 THY1 gene Proteins 0.000 description 2
- 208000035199 Tetraploidy Diseases 0.000 description 2
- 108070000030 Viral receptors Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- RJURFGZVJUQBHK-UHFFFAOYSA-N actinomycin D Natural products CC1OC(=O)C(C(C)C)N(C)C(=O)CN(C)C(=O)C2CCCN2C(=O)C(C(C)C)NC(=O)C1NC(=O)C1=C(N)C(=O)C(C)=C2OC(C(C)=CC=C3C(=O)NC4C(=O)NC(C(N5CCCC5C(=O)N(C)CC(=O)N(C)C(C(C)C)C(=O)OC4C)=O)C(C)C)=C3N=C21 RJURFGZVJUQBHK-UHFFFAOYSA-N 0.000 description 2
- 239000012190 activator Substances 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 230000003915 cell function Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 108091092356 cellular DNA Proteins 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 230000014107 chromosome localization Effects 0.000 description 2
- 238000012411 cloning technique Methods 0.000 description 2
- 239000013599 cloning vector Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000000326 densiometry Methods 0.000 description 2
- 229960000633 dextran sulfate Drugs 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 229960002743 glutamine Drugs 0.000 description 2
- 230000013595 glycosylation Effects 0.000 description 2
- 238000006206 glycosylation reaction Methods 0.000 description 2
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 230000002998 immunogenetic effect Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 210000002536 stromal cell Anatomy 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 210000001550 testis Anatomy 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 230000001052 transient effect Effects 0.000 description 2
- 230000005740 tumor formation Effects 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 108020004463 18S ribosomal RNA Proteins 0.000 description 1
- 108020005096 28S Ribosomal RNA Proteins 0.000 description 1
- 101150033839 4 gene Proteins 0.000 description 1
- 238000011640 AKR mouse Methods 0.000 description 1
- 241000881711 Acipenser sturio Species 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 108050001427 Avidin/streptavidin Proteins 0.000 description 1
- 208000012526 B-cell neoplasm Diseases 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 102100032912 CD44 antigen Human genes 0.000 description 1
- 108010032795 CD8 receptor Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 102000000844 Cell Surface Receptors Human genes 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 102000001493 Cyclophilins Human genes 0.000 description 1
- 108010068682 Cyclophilins Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 108010092160 Dactinomycin Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 201000009051 Embryonal Carcinoma Diseases 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 101800001467 Envelope glycoprotein E2 Proteins 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000009465 Growth Factor Receptors Human genes 0.000 description 1
- 108010009202 Growth Factor Receptors Proteins 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 101710088172 HTH-type transcriptional regulator RipA Proteins 0.000 description 1
- 102000006947 Histones Human genes 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 description 1
- 101001057504 Homo sapiens Interferon-stimulated gene 20 kDa protein Proteins 0.000 description 1
- 101001055144 Homo sapiens Interleukin-2 receptor subunit alpha Proteins 0.000 description 1
- 102100034353 Integrase Human genes 0.000 description 1
- 102100027268 Interferon-stimulated gene 20 kDa protein Human genes 0.000 description 1
- 102000000588 Interleukin-2 Human genes 0.000 description 1
- 108010002350 Interleukin-2 Proteins 0.000 description 1
- 108090000862 Ion Channels Proteins 0.000 description 1
- 102000004310 Ion Channels Human genes 0.000 description 1
- 102000006391 Ion Pumps Human genes 0.000 description 1
- 108010083687 Ion Pumps Proteins 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 241000288904 Lemur Species 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 108091027974 Mature messenger RNA Proteins 0.000 description 1
- 206010027541 Microgenia Diseases 0.000 description 1
- 241000713333 Mouse mammary tumor virus Species 0.000 description 1
- 101100441383 Mus musculus Slc7a2 gene Proteins 0.000 description 1
- 241001531264 Mus musculus musculus Species 0.000 description 1
- 230000004988 N-glycosylation Effects 0.000 description 1
- 229930193140 Neomycin Natural products 0.000 description 1
- 102000019315 Nicotinic acetylcholine receptors Human genes 0.000 description 1
- 108050006807 Nicotinic acetylcholine receptors Proteins 0.000 description 1
- 208000015914 Non-Hodgkin lymphomas Diseases 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 108020005497 Nuclear hormone receptor Proteins 0.000 description 1
- 102000007399 Nuclear hormone receptor Human genes 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 206010061535 Ovarian neoplasm Diseases 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 208000007913 Pituitary Neoplasms Diseases 0.000 description 1
- 241000282405 Pongo abelii Species 0.000 description 1
- 101000902592 Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) DNA polymerase Proteins 0.000 description 1
- 239000012979 RPMI medium Substances 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 102000001218 Rec A Recombinases Human genes 0.000 description 1
- 108010055016 Rec A Recombinases Proteins 0.000 description 1
- 101710138747 Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 102100038803 Somatotropin Human genes 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 101800001271 Surface protein Proteins 0.000 description 1
- 108020005038 Terminator Codon Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 102000001999 Transcription Factor Pit-1 Human genes 0.000 description 1
- 108010040742 Transcription Factor Pit-1 Proteins 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 108700005077 Viral Genes Proteins 0.000 description 1
- 108010066342 Virus Receptors Proteins 0.000 description 1
- 102000018265 Virus Receptors Human genes 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000001594 aberrant effect Effects 0.000 description 1
- 230000035508 accumulation Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- RJURFGZVJUQBHK-IIXSONLDSA-N actinomycin D Chemical compound C[C@H]1OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]2CCCN2C(=O)[C@@H](C(C)C)NC(=O)[C@H]1NC(=O)C1=C(N)C(=O)C(C)=C2OC(C(C)=CC=C3C(=O)N[C@@H]4C(=O)N[C@@H](C(N5CCC[C@H]5C(=O)N(C)CC(=O)N(C)[C@@H](C(C)C)C(=O)O[C@@H]4C)=O)C(C)C)=C3N=C21 RJURFGZVJUQBHK-IIXSONLDSA-N 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000001261 affinity purification Methods 0.000 description 1
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000030741 antigen processing and presentation Effects 0.000 description 1
- 210000000612 antigen-presenting cell Anatomy 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 108010058966 bacteriophage T7 induced DNA polymerase Proteins 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 150000001615 biotins Chemical class 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 239000003710 calcium ionophore Substances 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 239000002771 cell marker Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000001054 cortical effect Effects 0.000 description 1
- -1 cyclophyllin Proteins 0.000 description 1
- 102000003675 cytokine receptors Human genes 0.000 description 1
- 108010057085 cytokine receptors Proteins 0.000 description 1
- 238000004163 cytometry Methods 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- 229960000640 dactinomycin Drugs 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000010217 densitometric analysis Methods 0.000 description 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 1
- 229960003957 dexamethasone Drugs 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 108010078428 env Gene Products Proteins 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000004545 gene duplication Effects 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 208000019691 hematopoietic and lymphoid cell neoplasm Diseases 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 208000026278 immune system disease Diseases 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000004068 intracellular signaling Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 210000004324 lymphatic system Anatomy 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 230000001320 lysogenic effect Effects 0.000 description 1
- 230000036210 malignancy Effects 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000001394 metastastic effect Effects 0.000 description 1
- 206010061289 metastatic neoplasm Diseases 0.000 description 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 108091027963 non-coding RNA Proteins 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 230000009701 normal cell proliferation Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000005868 ontogenesis Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 210000001986 peyer's patch Anatomy 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 208000010916 pituitary tumor Diseases 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 1
- 238000010814 radioimmunoprecipitation assay Methods 0.000 description 1
- 230000013183 regulation of T cell differentiation Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 108020004418 ribosomal RNA Proteins 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 230000005100 tissue tropism Effects 0.000 description 1
- 230000002463 transducing effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Plant Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Compounds Of Unknown Constitution (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50968490A | 1990-04-13 | 1990-04-13 | |
| PCT/US1991/002518 WO1991016430A1 (en) | 1990-04-13 | 1991-04-12 | NOVEL T-CELL LYMPHOMA cDNA CLONES |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO923918D0 NO923918D0 (no) | 1992-10-08 |
| NO923918L NO923918L (no) | 1992-12-01 |
| NO307341B1 true NO307341B1 (no) | 2000-03-20 |
Family
ID=24027673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO923918A NO307341B1 (no) | 1990-04-13 | 1992-10-08 | Nye T-celle lymfom cDNA kloner og fremstilling av T- lymfomcelleprotein |
Country Status (22)
| Country | Link |
|---|---|
| US (2) | US5312733A (ja) |
| EP (1) | EP0527813B1 (ja) |
| JP (1) | JPH05506989A (ja) |
| KR (1) | KR100212371B1 (ja) |
| AT (1) | ATE141105T1 (ja) |
| AU (1) | AU664535B2 (ja) |
| CA (1) | CA2080018C (ja) |
| DE (1) | DE69121240T2 (ja) |
| DK (1) | DK0527813T3 (ja) |
| ES (1) | ES2093100T3 (ja) |
| FI (1) | FI108037B (ja) |
| GR (1) | GR3021478T3 (ja) |
| HK (1) | HK1006726A1 (ja) |
| IE (1) | IE75903B1 (ja) |
| IL (1) | IL97775A (ja) |
| NO (1) | NO307341B1 (ja) |
| NZ (1) | NZ237669A (ja) |
| PT (1) | PT97355B (ja) |
| RU (1) | RU2116346C1 (ja) |
| SA (1) | SA92120348B1 (ja) |
| WO (1) | WO1991016430A1 (ja) |
| ZA (1) | ZA912489B (ja) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6784163B1 (en) * | 1990-04-13 | 2004-08-31 | Macleod Carol L. | Inhibition of cationic amino acid transporter protein and uses thereof |
| WO1994004567A1 (en) * | 1992-08-21 | 1994-03-03 | La Jolla Cancer Research Foundation | A matrix-associating dna-protein, nucleic acids encoding the same and methods for detecting the nucleic acids |
| US7138500B1 (en) * | 1993-05-07 | 2006-11-21 | Immunex Corporation | Antibodies to human 4-1BB |
| WO1994026290A1 (en) * | 1993-05-07 | 1994-11-24 | Immunex Corporation | Cytokine designated 4-1bb ligand and human receptor that binds thereto |
| US7211259B1 (en) | 1993-05-07 | 2007-05-01 | Immunex Corporation | 4-1BB polypeptides and DNA encoding 4-1BB polypeptides |
| US5866329A (en) * | 1995-09-27 | 1999-02-02 | Demetriou; Achilles A. | Method and probe for detection of gene associated with liver neoplastic disease |
| US5861248A (en) * | 1996-03-29 | 1999-01-19 | Urocor, Inc. | Biomarkers for detection of prostate cancer |
| US20030096355A1 (en) * | 1999-07-09 | 2003-05-22 | Ke Zhang | Isolation, identification and characterization of ymkz5, a novel member of the TNF-receptor supergene family |
| US6627199B1 (en) * | 1999-07-09 | 2003-09-30 | Amgen Inc | Isolation, identification and characterization of tmst2, a novel member of the TNF-receptor supergene family |
| AU783682B2 (en) * | 1999-08-04 | 2005-11-24 | Amgen, Inc. | Fhm, a novel member of the TNF ligand supergene family |
| WO2001010908A1 (en) * | 1999-08-04 | 2001-02-15 | Amgen Inc. | Ntr3, a member of the tnf-receptor supergene family |
| WO2003027276A2 (en) * | 2001-09-24 | 2003-04-03 | University Of Aarhus | Novel compositions and methods for diagnosis and treatment of lymphoma and leukemia |
| US20100021953A1 (en) * | 2005-08-03 | 2010-01-28 | Astrazeneca Ab | Method for Identifying an Agent that Modulates Arginine Transport in a Chondrocyte |
| CN101313061B (zh) * | 2005-11-18 | 2013-05-15 | 大学健康网络 | 扩增双阴性t细胞的方法 |
| AU2010258094B2 (en) | 2009-06-10 | 2013-09-05 | Stephen Sanig Research Institute Ltd. | Methods of generating cells exhibiting phenotypic plasticity |
-
1991
- 1991-04-03 NZ NZ237669A patent/NZ237669A/xx unknown
- 1991-04-04 ZA ZA912489A patent/ZA912489B/xx unknown
- 1991-04-04 IL IL97775A patent/IL97775A/en not_active IP Right Cessation
- 1991-04-11 US US07/686,322 patent/US5312733A/en not_active Expired - Lifetime
- 1991-04-12 PT PT97355A patent/PT97355B/pt not_active IP Right Cessation
- 1991-04-12 DK DK91908417.8T patent/DK0527813T3/da active
- 1991-04-12 ES ES91908417T patent/ES2093100T3/es not_active Expired - Lifetime
- 1991-04-12 KR KR1019920702504A patent/KR100212371B1/ko not_active IP Right Cessation
- 1991-04-12 AT AT91908417T patent/ATE141105T1/de not_active IP Right Cessation
- 1991-04-12 EP EP91908417A patent/EP0527813B1/en not_active Expired - Lifetime
- 1991-04-12 AU AU77457/91A patent/AU664535B2/en not_active Ceased
- 1991-04-12 JP JP91508037A patent/JPH05506989A/ja active Pending
- 1991-04-12 IE IE125091A patent/IE75903B1/en not_active IP Right Cessation
- 1991-04-12 RU SU5053186/13A patent/RU2116346C1/ru not_active IP Right Cessation
- 1991-04-12 DE DE69121240T patent/DE69121240T2/de not_active Expired - Fee Related
- 1991-04-12 WO PCT/US1991/002518 patent/WO1991016430A1/en active IP Right Grant
- 1991-04-12 CA CA002080018A patent/CA2080018C/en not_active Expired - Fee Related
-
1992
- 1992-01-22 SA SA92120348A patent/SA92120348B1/ar unknown
- 1992-10-08 NO NO923918A patent/NO307341B1/no not_active IP Right Cessation
- 1992-10-09 FI FI924575A patent/FI108037B/fi not_active IP Right Cessation
-
1993
- 1993-01-11 US US08/002,999 patent/US5440017A/en not_active Expired - Fee Related
-
1996
- 1996-10-24 GR GR960402837T patent/GR3021478T3/el unknown
-
1998
- 1998-06-23 HK HK98106035A patent/HK1006726A1/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| HK1006726A1 (en) | 1999-03-12 |
| EP0527813A4 (en) | 1993-05-05 |
| EP0527813A1 (en) | 1993-02-24 |
| RU2116346C1 (ru) | 1998-07-27 |
| AU7745791A (en) | 1991-11-11 |
| IE911250A1 (en) | 1991-10-23 |
| US5440017A (en) | 1995-08-08 |
| IL97775A0 (en) | 1992-06-21 |
| DE69121240D1 (de) | 1996-09-12 |
| NO923918L (no) | 1992-12-01 |
| US5312733A (en) | 1994-05-17 |
| FI924575A (fi) | 1992-10-09 |
| ES2093100T3 (es) | 1996-12-16 |
| NO923918D0 (no) | 1992-10-08 |
| IE75903B1 (en) | 1997-10-08 |
| ATE141105T1 (de) | 1996-08-15 |
| IL97775A (en) | 1998-04-05 |
| FI108037B (fi) | 2001-11-15 |
| KR100212371B1 (ko) | 1999-08-02 |
| PT97355B (pt) | 1998-08-31 |
| EP0527813B1 (en) | 1996-08-07 |
| DE69121240T2 (de) | 1997-01-09 |
| WO1991016430A1 (en) | 1991-10-31 |
| GR3021478T3 (en) | 1997-01-31 |
| FI924575A0 (fi) | 1992-10-09 |
| CA2080018A1 (en) | 1991-10-14 |
| JPH05506989A (ja) | 1993-10-14 |
| SA92120348B1 (ar) | 2004-01-24 |
| ZA912489B (en) | 1992-01-29 |
| KR930700657A (ko) | 1993-03-15 |
| PT97355A (pt) | 1991-12-31 |
| CA2080018C (en) | 2003-10-14 |
| DK0527813T3 (da) | 1996-12-30 |
| AU664535B2 (en) | 1995-11-23 |
| NZ237669A (en) | 1992-08-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Prasad et al. | Leucine-zipper dimerization motif encoded by the AF17 gene fused to ALL-1 (MLL) in acute leukemia. | |
| US5342761A (en) | Oncofetal gene, gene product and uses therefor | |
| Lux et al. | Cloning and characterization of band 3, the human erythrocyte anion-exchange protein (AE1). | |
| Van den Eynde et al. | The gene coding for a major tumor rejection antigen of tumor P815 is identical to the normal gene of syngeneic DBA/2 mice. | |
| Sap et al. | Cloning and expression of a widely expressed receptor tyrosine phosphatase. | |
| Nagata et al. | Peptides derived from a wild-type murine proto-oncogene c-erbB-2/HER2/neu can induce CTL and tumor suppression in syngeneic hosts. | |
| Yamamura et al. | Functional expression of a microinjected E d α gene in C57BL/6 transgenic mice | |
| Holzbaur et al. | Homology of a 150K cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued | |
| NO307341B1 (no) | Nye T-celle lymfom cDNA kloner og fremstilling av T- lymfomcelleprotein | |
| Jefferies et al. | E3/19K from adenovirus 2 is an immunosubversive protein that binds to a structural motif regulating the intracellular transport of major histocompatibility complex class I proteins. | |
| Jiang et al. | Minimal truncation of the c-myb gene product in rapid-onset B-cell lymphoma | |
| Perelman et al. | Molecular cloning of a novel human gene encoding a 63-kDa protein and its sublocalization within the 11q13 locus | |
| Yoshikawa et al. | Isolation and characterization of mouse CD7 cDNA | |
| US20070141579A1 (en) | MUM-1 protein expressed by multiple myeloma-related gene | |
| EP0435877A1 (en) | Polynucleotides that encode the human proteoglycan peptide core of the effector cells of the immune response | |
| US20030040029A1 (en) | Detection of tumor marker transcript and protein recognized by naive natural killer cells | |
| US20030124603A1 (en) | Exocrine gland tight junction-constituting protein jeap family | |
| Fong | An in vitro model system which mimics the thymic microenvironment induces a novel gene in" maturing" T lymphoma cells | |
| JP2004073142A (ja) | Ｇａｎｐ結合性タンパク質 | |
| WO1997004010A9 (en) | SIGNAL TRANSDUCTION AGENTS OF T-CELL RECEPTOR Vα-1 GENE LOCUS |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM1K | Lapsed by not paying the annual fees |